Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/93059
標題: Effects of the ractopamine on the growth, body composition, in tilapia, Oreochromis mossambicus and residues
飼料中添加萊克多巴胺對吳郭魚之成長、體組之影響及殘留
作者: Chi-Chiao Feng
馮經喬
關鍵字: 萊克多巴胺
吳郭魚
ractopamine
tilapia
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摘要: 本研究之目的在於探討乙型受體素萊克多巴胺(RAC)對吳郭魚生長及體組成及殘留之影響,以及萊克多巴胺使用於吳郭魚的可能損害性和藥物動力學。將180隻吳郭魚苗隨機分成三組,分別為高劑量組(飼料中添加60 ppm RAC),低劑量組(飼料中添加30 ppm RAC)及陰性對照組(飼料中添加相同劑量之甲醇),連續餵食十二週。實驗結果顯示,餵食含30及60 ppm RAC飼料12週後,與對照組比對後於心臟並無明顯的病變發生。飼育實驗結果顯示,吳郭魚增重率以30 ppm處理組為最高(1,929 %)顯著高於對照組(1,506 %),而飼料中含30 ppm與60ppm處理組之增重無顯著差異。體組織蛋白以吳郭魚餵食60ppm之處理組為最高(89.05 %),而肌肉脂肪含量在對照組、低劑量組、高劑量組分別為6.53 %、4.99 %及4.79 %,隨著RAC添加量增加而有降低的趨勢。實驗結果顯示本研究所建立的HPLC檢測技術: Cosmosil 5C18-MS-II管柱(內徑 4.6 mm x 25 cm),移動相為7 mM醋酸銨溶液與乙睛以15:85 (v/v) (pH 7)、流速1.1 mL/min,紫外光偵測器之波長設定為194 nm。最低檢測極限可達0.01 ppm。此外藥物殘留試驗結果顯示在低劑量組(30 ppm),吳郭魚肌肉於停藥後1天濃度降至7.4 ppm,停藥後2天低於檢測極限0.01 ppm。肝臟於停藥後第8天濃度降至3.1 ppm,停藥後12天低於檢測極限0.01 ppm。在高劑量組(60 ppm),肌肉於停藥後2天濃度降至0.04 ppm,停藥後4天低於檢測極限0.01 ppm。肝臟於停藥後第8天濃度降至3.8 ppm,停藥後12天低於檢測極限0.01 ppm。RAC用於吳郭魚的T1/2為3.42 hr;Tmax為2.0 hr;Cmax為9.7 ppm;MRT為4.74 hr,Cl為964 Ml/hr/kg;AUC為337.8 hr × μg/Ml。綜合以上結果,萊克多巴胺在吳郭魚體內具有吸收及代謝快速,且不易殘留的特性,且在30及60 ppm濃度下均有促進生長效率及改善體組成的效果。
The aim of this study was to stady on the effects of the ractopamine hydrochloride (RAC) on the toxicity, growth performances, body composition, pharmacokinetics, and residues in tilapia. Total 180 tilapias were randomly separated into 3 groups on the following: high dose group (with 60 mg/ kg RAC), low dose group (with 30 mg/ kg RAC), and negative control group (with methanol as the same volume of RAC added). Both high dose and low dose groups were feed with RAC for 12 weeks. The developed HPLC method set with C18 coloume, acetonitrile : 7mM ammonium acetate solution (15 : 85) as a mobile phase (pH 7), flow rate 1.1 mL/min, and the UV-detector detects the max peak at wavelength 194 nm. The results showed that there was no significant lesions in myocardium and liver found in both high dose and low dose groups. However, both tested groups had significantly higher on weight gain compare with negative control group. Additionally, there was highest parameter of muscle protein in high dose group. But muscle lipid level decreased following with the dietary RAC level increasing were found. After given single dose 60 ppm bw/day of RAC via oral administration, the pharmacokinetic parameters T1/2, Cmax, MRT, Cl and AUC were 5.39 hr, 3 hr, 7.03 ppm, 9.4 hr, 884 ml/hr-kg, and 45 hr × μg/ml, respectively. We suggested that tilapia fed with diets supplemented with 30 and 60 ppm RAC had limited side effect, short withdral time, and positive effect on body weight gain, protein, and lipid levels in muscle tissue.
URI: http://hdl.handle.net/11455/93059
其他識別: U0005-1806201416312800
文章公開時間: 2017-12-16
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