Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/93081
標題: Characterization the Infection of Japanese Encephalitis Virus Genotype Ⅰ and Ⅲ
日本腦炎第一及第三基因型病毒感染特性分析
作者: 廖淑吟
Shu-Ying Liao
關鍵字: 日本腦炎
基因型
感染
Japanese Encephalitis Virus
JEV
Genotype
Flavivirus
引用: Abe, M., Kuzuhara, S., and Kino, Y., 2003, Establishment of an analyzing method for a Japanese encephalitis virus neutralization test in Vero cells. Vaccine 21, 1989-1994. Andrade CC, Maharaj PD, Reisen WK, Brault AC., 2011. North American West Nile virus genotype isolates demonstrate differential replicative capacities in response to temperature. J Gen Virol. 92(Pt 11):2523-33. Arai S, Matsunaga Y, Takasaki T, Tanaka-Taya K, Taniguchi K, Okabe N., 2008, Vaccine Preventable Diseases Surveillance Program of Japan. Japanese encephalitis: surveillance and elimination effort in Japan from 1982 to 2004. Jpn J Infect Dis 61:333–8. PMID:18806337 Benenson, M.W., Top, F.H., Gresso, W., Ames, C.W., Altstatt, L.B., 1975. The virulence to man of Japanese encephalitis virus in Thailand. Am. J. Trop. Med. Hyg. 24, 974–980. Boivin G, Hardy I, Tellier G, Maziade J., 2000. Predicting influenza infections during epidemics with use of a clinical case definition. Clin Infect Dis 31:1166-1169. Bressanelli S, Stiasny K, Allison SL, Stura EA, Duquerroy S, Lescar J, Heinz FX, Rey FA., 2004, Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation. EMBO J. 23:1–11. Buescher, E. L., and Scherer, W. F., 1959, Ecologic studies of Japanese encephalitis virus in Japan. IX. Epidemiologic correlations and conclusions. Am J Trop Med Hyg 8, 719-722. Burke, D. S. and Monath, T. P., 2001, Flaviviruses. In Fields Virology, 4 edn, pp. 1043-1125. Edited by D. M. Knipe & P. M. Howley. Philadelphia, Pa., USA:Lippincott-Williams & Wilkins. Burke, D. S., W. Lorsomrudee, C. J. Leake, C. H. Jr. Hoke, A. Nisalak, V. Chongswasdi, and T. Laorakpongse., 1985, Fatal outcome in Japanese encephalitis. Am. J. Trop Med. Hyg. 34: 1203-1210. Burns, K.F., 1950, Congenital Japanese B encephalitis infection of swine. Proc SocExp Biol Med 75, 621-625. Campbell GL, Hills SL, Fischer M, Jacobson JA, Hoke CH, Hombach JM, Marfin AA, Solomon T, Tsai TF, Tsu VD, Ginsburg AS. 2011. Estimated global incidence of Japanese encephalitis: a systematic review. Bull World Health Organ. 1;89(10):766-74. Chakraborty, M.S., Chakravarti, S.K., Mukherjee, K.K., Mitra, A.C., 1980. Inapparent infection by Japanese encephalitis (JE) virus in West Bengal. Indian J. Public Health 24, 121–127. Chan, Y. C., and T. F. Loh., 1966, Isolation of Japanese encephalitis virus from the blood of a child in Singapore. Am. J. Trop. Med. Hyg. 15(4):567-572. Chang, K. J., and T. C. Tseng. 1993. Seroepidemiological investigation on Japanese encephalitis in Taiwan (January 1989-December 1991). Chinese J. Microbiol. Immunol. 26(1): 25-37. Chang, K. J., and T. C. Tseng., 1993, Seroepidemiological investigation on Japanese encephalitis in Taiwan (January 1989-December 1991). Chinese J. Microbiol. Immunol. 26(1): 25-37. Chen WJ, Dong CF, Chiou LY, Chuang WL., 2000, Potential role of Armigeres subalbatus (Diptera: Culicidae) in the transmission of Japanese encephalitis virus in the absence of rice culture on Liu-Chiu islet, Taiwan. J Med Entomol, 37:108–13. Chen, W. J., C. F. Song, L. Y. Chiou, and W. L. Chung., 2000, Potential role of Armigeres subalbatus (Diptera: Culicidae) in the transmission of Japanese encephalitis virus in the absence of rice culture on Liu-Chiu Islet, Taiwan. J. Med. Entomol. 37(1): 108-113. Chen, W.R., Rico-Hesse, R., Tesh, R.B., 1992, A new genotype of Japanese encephalitis virus from Indonesia. Am J Trop Med Hyg 47, 61-69. Chen, W.R., Tesh, R.B., Rico-Hesse, R., 1990, Genetic variation of Japanese encephalitis virus in nature. J Gen Virol 71 ( Pt 12), 2915-2922. Chen, Y.Y., Fan, Y.C., Tu, W.C., Chang, R.Y., Shih, C.C., Lu, I.H., Chien, M.S., Lee, W.C., Chen, T.H., Chang, G.J. and Chiou, S.S. 2011, Japanese Encephalitis Virus Genotype Replacement, Taiwan, 2009–2010. Emerg Infect Dis 17, 2354-2356. Chiou SS, and Chen WJ. 2001. Mutation in the NS3 gene and 3'-NCR of Japanese encephalitis virus isolated from an unconventional ecosystem and implications for natural attenuation of the virus. Virology 289: 129-136. Clarke, D. H., and Casals, J., 1958, Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg 7, 561. Endy TP, Nisalak A, Chunsuttitwat S, Vaughn DW, Green S, Ennis FA, Rothman AL, Libraty DH. 2004. Relationship of preexisting dengue virus (DV) neutralizing antibody levels to viremia and severity of disease in a prospective cohort study of DV infection in Thailand. J Infect Dis. 15;189(6):990-1000. Endy, T. P., K. B. Anderson, A. Nisalak, I. K. Yoon, S. Green, A. L. Rothman, S. J. Thomas, R. G. Jarman, D. H. Libraty, and R. V. Gibbons. 2011. Determinants of inapparent and symptomatic dengue infection in a prospective study of primary school children in Kamphaeng Phet, Thailand. PLoS neglected tropical diseases 5:e975. Fan YC, Chen JM, Chen YY, Lin JW, Chiou SS. , 2013, Reduced neutralizing antibody titer against genotype I virus in swine immunized with a live-attenuated genotype III Japanese encephalitis virus vaccine. Vet Microbiol. 3;163(3-4):248-56. Fan, Y. C., J. M. Chen, H. C. Chiu, Y. Y. Chen, J. W. Lin, C. C. Shih, C. M. Chen, C. C. Chang, G. J. Chang, and S. S. Chiou. 2012. Partially neutralizing potency against emerging genotype I virus among children received formalin-inactivated Japanese encephalitis virus vaccine. PLoS neglected tropical diseases 6:e1834. Gollins SW, Porterfield JS., 1984, Flavivirus infection enhancement in macrophages: radioactive and biological studies on the effect of antibody on viral fate. J. Gen. Virol., 65:1261–1272. Grayston, J. T., Wang, S. P., and Yen, C. H., 1962, Encephalitis on Taiwan. I. Introduction and epidemiology. Am J Trop Med Hyg 11, 126-130. Grossberg, S. E., and W. F. Scherer. 1966. The effect of host age, virus dose and route of inoculation on inapparent infection in mice with Japanese encephalitis virus. Proc Soc Exp Biol Med 123:118-124. Grossman, R.A., Edelman, R., Willhight, M., Pantuwatana, S., Udomsakdi, S., 1973. Study of Japanese encephalitis virus in Chiangmai Valley, Thailand. 3. Human seroepidemiology and inapparent infections. Am. J. Epidemiol. 98 (2), 133–149. Halstead, S.B., Grosz, C.R., 1962. Subclinical Japanese encephalitis. Infection of Americans with limited residence in Korea. Am. J. Hyg. 75, 190–201. Hammon, W. M., Tigertt, W. D., Sather, G. E., Berge, T. O., and Meiklejohn, G., 1958, Epidemiologic studies of concurrent 'virgin' epidemics of Japanese B encephalitis and of mumps on Guam, 1947-1948, with subsequent observations including dengue, through 1957. Am J Trop Med Hyg 7, 441. Hasegawa, H., Yoshida, M., Fujita, S., Kobayashi, Y., 1994, Comparison of structural proteins among antigenically different Japanese encephalitis virus strains. Vaccine 12, 841-844. Hoke CH, Nisalak A, Sangawhipa N, Jatanasen S, Laorakapongse T, Innis BL et al. 1988. Protection against Japanese encephalitis by inactivated vaccines. N Engl J Med 319:608–14. Hsieh, W. C., Wang, S. P., and Rasmussen, A. F., 1961, Epidemiology of Japanese encephalitis (JE) on Taiwan in 1960. J Formos Med Assoc 60, 825-830. Hsu, S.H., Huang, W.C., Cross, J.H., 1978, The isolation of Japanese encephalitis virus from Taiwan mosquitoes by mosquito cell cultures and mouse inoculation. J Med Entomol 14, 698-701. Hsu, T. C., L. P. Chow, H. Y. Wei, C. L. Chen, S. T. Hsu, C. T. Huang, M. Kitaoka, and H. Sunaga., 1971, A controlled field trial for an evaluation of effectiveness of mouse-brain Japanese encephalitis vaccine. J. Formosan Med. Assoc. 70: 55-62. Huang, C.H., 1982. Studies of Japanese encephalitis in China. Adv. Virus. Res. 27, 71–101. Huang, J.H., Lin, T.H., Teng, H.J., Su, C.L., Tsai, K.H., Lu, L.C., Lin, C., Yang, C.F., Chang, S.F., Liao, T.L., Yu, S.K., Cheng, C.H., Chang, M.C., Hu, H.C., Shu, P.Y., 2010, Molecular epidemiology of Japanese encephalitis virus, Taiwan. Emerg Infect Dis 16, 876-878. Ishak R, Tovey DG, Howard CR., 1988, Morphogenesis of yellow fever virus 17D in infected cell cultures. J. Gen. Virol. 69:325–335. Kalita, J., U. K. Misra, S. Pandey, and T. N. Dhole., 2003, A comparison of clinical and radiological findings in adults and children with Japanese encephalitis. Arch. Neurol. 60: 1760-1764. Kielian M., 2006, Class II virus membrane fusion proteins. Virology. 344(1):38-47. Kono, R., and K. H. Kim., 1969, Comparative epidemiological features of Japanese encephalitis in the Republic of Korea, China (Taiwan) and Japan. Bull World Health Organ. 40: 263-277. Kuno, G. 2003. Serodiagnosis of flaviviral infections and vaccinations in humans. Advances in virus research 61:3-65. Lanciotti,R.S., Roehrig, J. T.,Deubel, V.,Smith, J., Parker,M., Steele,K.,Crise,B., Volpe, K. E.,Crabtree, M. B. & other authors., 1999. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States. Science 286, 2333–2337. Liou, M. L., and C. Y. Hsu., 1998, Japanese encephalitis virus is transported across the cerebral blood vessels by endocytosis in mouse brain. Cell Tissue Res. 293: 389-394. Mason, P. W., S. Pincus, and M. J. Fournier., 1991, Japanese encephalitis virus-vaccinia recombinants produce particulate forms of the structural proteins and induce high levels of protection against lethal JEV infection. Virology. 180: 294-305. Nabeshima, T., Loan, H.T., Inoue, S., Sumiyoshi, M., Haruta, Y., Nga, P.T., Huoung, V.T., del Carmen Parquet, M., Hasebe, F., Morita, K., 2009, Evidence of frequent introductions of Japanese encephalitis virus from south-east Asia and continental east Asia to Japan. J Gen Virol 90, 827-832. Nga, P.T., del Carmen Parquet, M., Cuong, V.D., Ma, S.P., Hasebe, F., Inoue, S., Makino, Y., Takagi, M., Nam, V.S., Morita, K., 2004, Shift in Japanese encephalitis virus (JEV) genotype circulating in northern Vietnam: implications for frequent introductions of JEV from Southeast Asia to East Asia. J Gen Virol 85, 1625-1631. Ni, H., Barrett, A.D., 1995, Nucleotide and deduced amino acid sequence of the structural protein genes of Japanese encephalitis viruses from different geographical locations. J Gen Virol 76 ( Pt 2), 401-407. Nitatpattana, N., Dubot-Peres, A., Gouilh, M.A., Souris, M., Barbazan, P., Yoksan, S., de Lamballerie, X., Gonzalez, J.P., 2008, Change in Japanese encephalitis virus distribution, Thailand. Emerg Infect Dis 14, 1762-1765. Okuno Y, Fukunaga T, Tadano M, Okamoto Y, Ohnishi T, Takagi M. 1985. Rapid focus reduction neutralization test of Japanese encephalitis virus in microtiter system. Brief report. Arch Virol. 86(1-2):129-35. Okuno, Y., Igarashi, A., and Fukai, K., 1978, Neutralization tests for dengue and Japanese encephalitis viruses by the focus reduction method using peroxidase-anti-peroxidase staining. Biken J 21, 137-147. Olsen SJ, Supawat K, Campbell AP, Anantapreecha S, Liamsuwan S, Tunlayadechanont S, Visudtibhan A, Lupthikulthum S, Dhiravibulya K, Viriyavejakul A, Vasiknanonte P, Rajborirug K, Watanaveeradej V, Nabangchang C, Laven J, Kosoy O, Panella A, Ellis C, Henchaichon S, Khetsuriani N, Powers AM, Dowell SF, Fischer M. 2010. Japanese encephalitis virus remains an important cause of encephalitis in Thailand. Int J Infect Dis. 14(10):e888-92. Perera R, Khaliq M, Kuhn RJ., 2008, Closing the door on flaviviruses: entry as a target for antiviral drug design. Antiviral Res. 80(1):11-22 Saxena, S.K., Tiwari, S., Saxena, R., Mathur, A. and Nair, M.P.N., 2011, Japanese Encephalitis: an Emerging and Spreading Arbovirosis: In Flavivirus Encephalitis (Book), Daniel Ruzek (Ed.), ISBN 979-953-307-775-7, InTech, Croatia (European Union), 295-316 Simpson, T. W., and G. Meiklejohn., 1947, Sequelae of Japanese B encephalitis. Am. J. Trop. Med. Hyg. 27: 727-730. Snapinn, K.W., Holmes, E.C., Young, D.S., Bernard, K.A., Kramer, L.D., Ebel, G.D., 2007. Declining growth rate of West Nile virus in North America. J Virol 81,2531-2534. Solomon, T., 1997, Viral Encephalitis in Southeast Asia. Neurological Infections and Epidemiology. 2: 191-199. Solomon, T., and D. W. Vaughn., 2002, Pathogenesis and clinical features of Japanese encephalitis and West Nile virus infections. Curr. Top. Microbiol. Immunol. 267: 171-94. Solomon, T., N. M. Dung, R. Kneen, L. T. Thao, M. Gainsborough, A. Nisalak, N. J. Day, F. J. Kirkham, D. W. Vaughn, S. Smith, and N. J. White., 2002, Seizures and raised intracranial pressure in Vietnamese patients with Japanese encephalitis. Brain 125: 1084-1093. Solomon, T., N. M. Dung, R. Kneen, M. Gainsborough, D. W. Vaughn, and V. T. Khanh., 2000, Neurological aspects of tropical diseases:Japanese encephalitis. J. Neurol. Neurosurg. Psychiatry. 68: 405-415. Solomon, T., Ni, H., Beasley, D.W., Ekkelenkamp, M., Cardosa, M.J., Barrett, A.D., 2003, Origin and evolution of Japanese encephalitis virus in southeast Asia. J Virol 77, 3091-3098. Solomon, T., Winter, P.M., 2004. Neurovirulence and host factors in flavivirus encephalitis evidence from clinical epidemiology. Arch. Virol. Suppl. 18,161–170. Tajima S, Nerome R, Nukui Y, Kato F, Takasaki T, Kurane I. 2010. A single mutation in the Japanese encephalitis virus E protein (S123R) increases its growth rate in mouse neuroblastoma cells and its pathogenicity in mice. Virology. 2010 Jan 20;396(2):298-304. Takashima, I., Watanabe, T., Ouchi, N., Hashimoto, N., 1988, Ecological studies of Japanese encephalitis virus in Hokkaido: interepidemic outbreaks of swine abortion and evidence for the virus to overwinter locally. Am J Trop Med Hyg 38, 420-427. Tseng HF, Tan HF, Chang CK, Huang WL, Ho WC., 2003. Seroepidemiology study of Japanese encephalitis neutralizing antibodies in southern Taiwan: A comparative study between urban city and country townships. Am J Infect Control. 31(7):435-40. Uchil, P.D., Satchidanandam, V., 2001, Phylogenetic analysis of Japanese encephalitis virus: envelope gene based analysis reveals a fifth genotype, geographic clustering, and multiple introductions of the virus into the Indian subcontinent. Am J Trop Med Hyg 65, 242-251. van den Hurk, A.F., Ritchie, S.A., Mackenzie, J.S., 2009, Ecology and geographical expansion of Japanese encephalitis virus. Annu Rev Entomol 54, 17-35. Van Gessel, Y., C. S. Klade, R. Putnak, A. Formica, S. Krasaesub, M. Spruth, B. Cena, A. Tungtaeng, M. Gettayacamin, and S. Dewasthaly. 2011. Correlation of protection against Japanese encephalitis virus and JE vaccine (IXIARO((R))) induced neutralizing antibody titers. Vaccine 29:5925-5931. Vaughn, D.W., Hoke, C.H., Jr., 1992, The epidemiology of Japanese encephalitis: prospects for prevention. Epidemiol Rev 14, 197-221. Venturi, G., Mel, R., Marchi, A., Mancuso, S., Russino, F., Pra, G. D., Papa, N., Bertiato, G., Fiorentini, C., and Ciufolini, M. G., 2006, Humoral immunity and correlation between ELISA, hemagglutination inhibition, and neutralization tests after vaccination against tick-borne encephalitis virus in children. Journal of Virological Methods 134, 136-139. Wang T, Lin C, King C, Lee W., 2010. Estimating pathogen-specific asymptomatic ratios . Epidemiology.21:726 –728. Wang, H.Y., Takasaki, T., Fu, S.H., Sun, X.H., Zhang, H.L., Wang, Z.X., Hao, Z.Y., Zhang, J.K., Tang, Q., Kotaki, A., Tajima, S., Liang, X.F., Yang, W.Z., Kurane, I., Liang, G.D., 2007, Molecular epidemiological analysis of Japanese encephalitis virus in China. J Gen Virol 88, 885-894. Wang, S.P., Grayston, J.T., Hu, S.M., 1962, Encephalitis on Taiwan. III. Virus isolations from mosquitoes. Am J Trop Med Hyg 11, 141-148. Webb, J. K. G., and S. Perriera., 1956, Clinical diagnosis of an arthropod borne type virus encephalitis in children in North Arcot District, Madras State, India. Indian J. Med. Sci. 10: 573-581. Weng, M. H., J. C. Lien, C. C. Lin, and C. W. Yao., 2000, Vector competence of Culex pipiens molestus (Diptera: Culicidae) from Taiwan for a sympatric strain of Japanese encephalitis virus. J. Med. Entomol. 37(5): 780-783. Weng, M. H., J. C. Lien, Y. M. Wang, H. L. Wu, and C. Chin., 1997, Susceptibility of three laboratory strains of Aedes albopictus (Diptera:Culicidae) to Japanese encephalitis virus from Taiwan. J. Med. Entomol. 34(6): 745-747. Yamaguchi Y, Nukui Y, Kotaki A, Sawabe K, Saijo M, Watanabe H, Kurane I, Takasaki T, Tajima S. 2013. Characterization of a serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein. J Gen Virol. 94(Pt 1):90-6. Yu I, Zhang W, Holdaway HA, Li L, Kostyuchenko VA, Chipman PR, Kuhn RJ, Rossmann MG, Chen J., 2008, Structure of the immature dengue virus at low pH primes proteolytic maturation. Science. 319:1834–1837 Yun, S.M., Cho, J.E., Ju, Y.R., Kim, S.Y., Ryou, J., Han, M.G., Choi, W.Y., Jeong, Y.E., 2010, Molecular epidemiology of Japanese encephalitis virus circulating in South Korea, 1983-2005. Virol J 7, 127.
摘要: Japanese encephalitis virus (JEV) is a member of the Flavivirus genus of the family of Flaviviridae. Since 1990s, the JEV genotype III replaced by genotype I had been indicated in several southeast Asia Countries. According to the virological surveillance and molecular epidemiology of JEV among pig farms in Taiwan, the results also shown that the dominant JEV genotype in Taiwan has switched from genotype Ⅲ to Ⅰduring 2008 to 2009. In Japan, after JEV genotype shift, the JE confirmed cases were decreased from 50~100 to less than 10 cases annually, and suggested the genotype I JEVs were less virulence, and then the JEV vaccination program was ceased in 2005. However, the decreasing of confirmed case was not observed after JEV genotype replacement in Taiwan and Thailand. Also, some studies also indicated that there were no significant differences between genotype I and III in mouse neurovirulence. Thus, the possible mechanism(s) of JEV genotype III replaced by genotype I and the pathogenicity of genotype I and III in human are unclear. In this study, we estimate and compare the pathogenicity of genotype Ⅰ and Ⅲ by calculating the asymptomatic ratio and study infectivity of genotype I and III JEVs in different cell lines under different conditions. We collect the serum specimens during genotype Ⅰ(2010-2011) and genotype Ⅲ (1994 and 1999) endemic periods in Taiwan. The status of JEV infection was serologically determined using plaque reduction neutralization assay (PRNT) and IgM antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA). The asymptomatic ratio was estimated using JE confirmed cases as denominator and JEV infected cases as the numerator. The values were adjusted by JE uninfected cases with manifestation of encephalitis, gander and vaccination coverage using the log-linear binomial regression model. The results shown that the asymptomatic ratio of JEV GⅢ and GⅠ virus infection were 1:7,510±1,270 and 1:6,211±722.6, respectively. Based on the similar asymptomatic ratios of JEV GⅢ and GⅠ, we suggested the pathogenicity in human was similar between JEV genotype Ⅰ and Ⅲ virus. The replication of genotype I JEVs were better than genotype III viruses in DF-1, CER, DE and PK-15 cell lines, but not in C6/36 cells. Based on these results, we suggested that the genotype JEVs might have replication advantage in amplification hosts, but in vivo studies were needed.
日本腦炎病毒(Japanese encephalitis virus)屬於黃病毒科(Flaviviridae)、黃病毒屬(Flavivirus)之病毒。自1990 年代以來,許多東南亞國家流行的日本腦炎病毒發生由原本的第三型基因型轉變成以第一型基因型;台灣於2009~2010年間,利用對豬場與病媒蚊監測進行分子流行病學分析後,也發現於台灣亦已發生並完成基因型轉型之現象。日本在發生日本腦炎病毒基因型別轉變後,確定病例數由每年50~100例銳減至10例以下,並由此觀察結果此推論第一型基因型病毒致病能力較第三型基因型為弱,並且於2005年終止日本腦炎國家疫苗接種計畫。然而反觀台灣與泰國等其他國家,並未在發生日本腦炎病毒基因型轉型後觀察到日本腦炎確定病例下降之現象;且在小鼠之毒力試驗的相關研究中,亦無發現日本腦炎病毒不同基因型之毒力有顯著差異。僅就上述觀察及文獻之結果,日本腦炎病毒第一型取代第三型病毒機制,及此二基因型病毒在人類之致病力是否有所不同仍待研究。 本研究利用日本腦炎病毒第一型與第三型之不顯感染比例估算,以評估日本腦炎病毒第一型與第三型在人類之致病力是否有所不同;並利用不同條件感染於不同宿主細胞株之病毒生長曲線之進行比較,以探討在傳播途徑之不同宿主中日本腦炎第一基因型病毒取代第三型基因型病毒之可能機轉及。本研究中利用在第三基因型流行時所收集之血清檢體(1994、1999年)與第一基因型流行時所收集之血清檢體(2010-2011年)利用MAC-ELISA及PRNT50來估算該地區日本腦炎盛行率並計算其不顯感染比例(確定病例數比被感染數),並針對背景症狀、性別、疫苗接種情況利用Log-linear binomial regression model進行校正,得到台灣日本腦炎病毒之不顯感染比例第三型與第一型分別為1:7,510±1,270 與 1:6,211±722.6,發現以此不顯感染比例比較第一基因型與第三型基因型病毒在感染台灣居民後之致病力並無顯著差異。而病毒生長曲線部分,在禽類細胞DF-1、CER、DE細胞株中皆可以觀察到第一基因型日本腦炎病毒複製情形優於部分第三型基因型病毒,在以高溫培養的豬隻PK-15細胞株中亦發現一基因型日本腦炎病毒複製情形優於部分第三型基因型病毒之情形。但是在白線斑蚊C6/36細胞株則發現不論在不同溫度或是感染劑量之條件皆無顯著差異。由此結果推論,在日本腦炎第一基因型病毒取代第三型基因型病毒之可能機轉中,日本腦炎第一型與第三型基因型病毒於增幅宿主(豬及鳥類)中之適應性與複製能力之差異可能為重要之原因之一,但仍須進一步進行動物實驗。
URI: http://hdl.handle.net/11455/93081
文章公開時間: 2018-08-31
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