Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/93083
標題: Comparative Analysis of Anti-influenza Virus Activity Mediated by Monoacetylcurcumin and Curcumin
比較分析單乙醯薑黃素以及薑黃素之抗流感病毒機制
作者: 李宜霖
Yi-Lin Li
關鍵字: 
no
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'Inhibition of influenza A virus (H1N1) fusion by benzenesulfonamide derivatives targeting viral hemagglutinin.' PLoS One 6 (12):e29120. doi: 10.1371/journal.pone.0029120.
摘要: Curcumin (Cur) is a commonly used coloring agent and spice in food. Previously, we reported curcumin blocks influenza A virus (IAV) activity via multiple mechanisms. By comparing the effect of curcumin analogues on IAV infection, monoacetylcurcumin (MAC) harbors a similar anti-IAV strength to curcumin. However, unlike curcumin, MAC did not inhibit viral HA activity, indicating that MAC possibly blocks IAV via a different mechanism. Hence, in the study herein, effect of these two compounds on several aspects related to IAV infection was compared. The results showed that as with curcumin, MAC attacked membrane structure in the liposome system. In NA inhibition assay, curcumin and MAC interfered with the activity of NA. Curcumin and MAC have been reported to inhibit Akt and NF-B pathways, two of cellular signaling pathways activated by IAV and are required for IAV replication. Treatment of curcumin analogues after virus entry indicated that curcumin and MAC inhibited phosphorylation (activation) of Akt and NF-B to a similar extent. Results of hemolysis inhibition assay and plaque reduction assay indicated curcumin and MAC inhibited membrane fusion activity. Finally, results of mini-replicon assay showed that treatment of curcumin or MAC inhibited viral RNA translation and transcription. Taken together, as a strong inhibitor of IAV, MAC shares similar effect to curcumin, but it does not inhibit HA activity.
薑黃素(Curcumin)為薑黃(Curcuma longa)之萃取物,可做為食物香料使用,目前研究已知curcumin 具有抗腫瘤、抗發炎、抗氧化、增強免疫力以及預防阿茲海默症的能力。實驗室先前研究發現curcumin 會透過干擾HA 的功能達到抑制流感病毒活性, 比較分析curcumin 結構類似物的抗流感病毒能力顯示:monoacetylcurcumin (MAC)具有與curcumin 不相上下的抗流感病毒能力,但是MAC 並不抑制流感病毒蛋白HA 的功能,而且在病毒感染後給予藥物依舊能強烈地抑制流感病毒增殖,顯現curcumin 除了抑制病毒蛋白HA 外還有其他抗流感機制,本研究透過一系列的實驗分析比較curcumin 與MAC 的抗流感機轉。以微脂體(liposome)模擬病毒實驗得知,curcumin 與MAC 均具有破壞微脂體的能力,此外,NA 封套蛋白的功能也會受curcumin 與MAC 所干擾。已知curcumin 與MAC可以抑制細胞訊息蛋白Akt、NF-b 的活化,而流感病毒感染時會藉由活化這兩條細胞訊息路徑來提高感染效率;本研究透過西方墨點法來觀測curcumin 與MAC抑制流感病毒感染活化Akt 與NF-b 的能力,結果證實這兩者藥物明顯抑制細胞中因為流感病毒感染而磷酸化的Akt 與NF-b 蛋白量。透過溶血抑制分析以及不同感染時間點進行藥物處理的方法來研究curcumin 與MAC 對於病毒與細胞融合的影響,結果證實處理藥物可以降低病毒與細胞融合,而使流感病毒無法進行後續的感染。另藉由mini-replicon assay 得知curcumin 與MAC均會干擾流感病毒RNA在細胞中的轉錄及轉譯。綜上所述,curcumin 與MAC 有相近的抗流感病毒能力,能透過不同的抗流感機制達到抑制流感病毒感染的目的。
URI: http://hdl.handle.net/11455/93083
文章公開時間: 2018-07-16
Appears in Collections:微生物暨公共衛生學研究所

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