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標題: Comparative Analysis of Anti-influenza Virus Activity Mediated by Monoacetylcurcumin and Curcumin
作者: 李宜霖
Yi-Lin Li
引用: 歐俊麟. 2013. '比較分析薑黃素及其類似物的抗流感病毒活性.' 碩士, 微生物暨公共衛生學研究所, 中興大學. Aggarwal, B. B., A. Kumar, and A. C. Bharti. 2003. 'Anticancer potential of curcumin: preclinical and clinical studies.' Anticancer Res 23 (1A):363‐98. Baudin, F., I. Petit, W. Weissenhorn, and R. W. Ruigrok. 2001. 'In vitro dissection of the membrane and RNP binding activities of influenza virus M1 protein.' Virology 281 (1):102‐8. doi: 10.1006/viro.2000.0804. Ben‐Yedidia, T., and R. Arnon. 2005. 'Towards an epitope‐based human vaccine for influenza.' Hum Vaccin 1 (3):95‐101. Bouvier, N. M., and P. Palese. 2008. 'The biology of influenza viruses.' Vaccine 26 Suppl 4:D49‐53. Brouet, I., and H. Ohshima. 1995. 'Curcumin, an anti‐tumour promoter and anti‐inflammatory agent, inhibits induction of nitric oxide synthase in activated macrophages.' Biochem Biophys Res Commun 206 (2):533‐40. Bui, M., G. Whittaker, and A. Helenius. 1996. 'Effect of M1 protein and low pH on nuclear transport of influenza virus ribonucleoproteins.' J Virol 70 (12):8391‐401. Caton, A. J., and J. S. Robertson. 1980. 'Structure of the host‐derived sequences present at the 5' ends of influenza virus mRNA.' Nucleic Acids Res 8 (12):2591‐603. Chang, S. L., Y. M. Chiang, C. L. Chang, H. H. Yeh, L. F. Shyur, Y. H. Kuo, T. K. Wu, and W. C. Yang. 2007. 'Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN‐gamma expression.' J Ethnopharmacol 112 (2):232‐6. doi: 10.1016/j.jep.2007.03.001. Chen, Da‐Yuan, Jui‐Hung Shien, Laurence Tiley, Shyan‐Song Chiou, Sheng‐Yang Wang, Tien‐Jye Chang, Ya‐Jane Lee, Kun‐Wei Chan, and Wei‐Li Hsu. 2010. 'Curcumin inhibits influenza virus infection and haemagglutination activity.' Food Chemistry 119 (4):1346‐1351. doi: Chen, T. Y., D. Y. Chen, H. W. Wen, J. L. Ou, S. S. Chiou, J. M. Chen, M. L. Wong, and W. L. Hsu. 2013. 'Inhibition of enveloped viruses infectivity by curcumin.' PLoS One 8 (5):e62482. doi: 10.1371/journal.pone.0062482. De Clercq, E. 2006. 'Antiviral agents active against influenza A viruses.' Nat Rev Drug Discov 5 (12):1015‐25. doi: 10.1038/nrd2175. Deodhar, S. D., R. Sethi, and R. C. Srimal. 1980. 'Preliminary study on antirheumatic activity of curcumin (diferuloyl methane).' Indian J Med Res 71:632‐4. Digard, P., V. C. Blok, and S. C. Inglis. 1989. 'Complex formation between influenza virus polymerase proteins expressed in Xenopus oocytes.' Virology 171 (1):162‐9. Dinkova‐Kostova, A. T., and P. Talalay. 1999. 'Relation of structure of curcumin analogs to their potencies as inducers of Phase 2 detoxification enzymes.' Carcinogenesis 20 (5):911‐4. Drake, J. W. 1993. 'Rates of spontaneous mutation among RNA viruses.' Proc Natl Acad Sci U S A 90 (9):4171‐5. Ehrhardt, C., and S. Ludwig. 2009. 'A new player in a deadly game: influenza viruses and the PI3K/Akt signalling pathway.' Cell Microbiol 11 (6):863‐71. doi: 10.1111/j.1462‐5822.2009.01309.x. Ehrhardt, C., H. Marjuki, T. Wolff, B. Nurnberg, O. Planz, S. Pleschka, and S. Ludwig. 2006. 'Bivalent role of the phosphatidylinositol‐3‐kinase (PI3K) during influenza virus infection and host cell defence.' Cell Microbiol 8 (8):1336‐48. doi: 10.1111/j.1462‐5822.2006.00713.x. Flory, E., M. Kunz, C. Scheller, C. Jassoy, R. Stauber, U. R. Rapp, and S. Ludwig. 2000. 'Influenza virus‐induced NF‐kappaB‐dependent gene expression is mediated by overexpression of viral proteins and involves oxidative radicals and activation of IkappaB kinase.' J Biol Chem 275 (12):8307‐14. Fodor, E., D. C. Pritlove, and G. G. Brownlee. 1994. 'The influenza virus panhandle is involved in the initiation of transcription.' J Virol 68 (6):4092‐6. Gao, R., B. Cao, Y. Hu, Z. Feng, D. Wang, W. Hu, J. Chen, Z. Jie, H. Qiu, K. Xu, X. Xu, H. Lu, W. Zhu, Z. Gao, N. Xiang, Y. Shen, Z. He, Y. Gu, Z. Zhang, Y. Yang, X. Zhao, L. Zhou, X. Li, S. Zou, Y. Zhang, X. Li, L. Yang, J. Guo, J. Dong, Q. Li, L. Dong, Y. Zhu, T. Bai, S. Wang, P. Hao, W. Yang, Y. Zhang, J. Han, H. Yu, D. Li, G. F. Gao, G. Wu, Y. Wang, Z. Yuan, and Y. Shu. 2013. 'Human infection with a novel avian‐origin influenza A (H7N9) virus.' N Engl J Med 368 (20):1888‐97. doi: 10.1056/NEJMoa1304459. Garcia‐Sastre, A. 2013. 'Lessons from lipids in the fight against influenza.' Cell 154 (1):22‐3. doi: 10.1016/j.cell.2013.06.024. Hatcher, H., R. Planalp, J. Cho, F. M. Torti, and S. V. Torti. 2008. 'Curcumin: from ancient medicine to current clinical trials.' Cell Mol Life Sci 65 (11):1631‐52. doi:10.1007/s00018‐008‐7452‐4. Hay, A. J., V. Gregory, A. R. Douglas, and Y. P. Lin. 2001. 'The evolution of human influenza viruses.' Philos Trans R Soc Lond B Biol Sci 356 (1416):1861‐70. doi: 10.1098/rstb.2001.0999. Hayden, F. G. 2006. 'Antiviral resistance in influenza viruses‐‐implications for management and pandemic response.' N Engl J Med 354 (8):785‐8. doi: 10.1056/NEJMp068030. Hilleman, M. R. 2002. 'Realities and enigmas of human viral influenza: pathogenesis, epidemiology and control.' Vaccine 20 (25‐26):3068‐87. Hoffmann, H. H., A. Kunz, V. A. Simon, P. Palese, and M. L. Shaw. 2011. 'Broad‐spectrum antiviral that interferes with de novo pyrimidine biosynthesis.' Proc Natl Acad Sci U S A 108 (14):5777‐82. doi: 10.1073/pnas.1101143108. Holmes, E. C., D. J. Lipman, D. Zamarin, and J. W. Yewdell. 2006. 'Comment on 'Large‐scale sequence analysis of avian influenza isolates'.' Science 313 (5793):1573; author reply 1573. doi: 10.1126/science.1131729. Jiang, M. C., H. F. Yang‐Yen, J. J. Yen, and J. K. Lin. 1996. 'Curcumin induces apoptosis in immortalized NIH 3T3 and malignant cancer cell lines.' Nutr Cancer 26 (1):111‐20. doi: 10.1080/01635589609514468. Kido, H., Y. Okumura, H. Yamada, T. Q. Le, and M. Yano. 2007. 'Proteases essential for human influenza virus entry into cells and their inhibitors as potential therapeutic agents.' Curr Pharm Des 13 (4):405‐14. Kim, K. J., E. S. Park, H. J. Chang, M. Suh, and D. W. Rha. 2013. 'Novel Influenza A (H1N1)‐Associated Acute Necrotizing Encephalopathy: A Case Report.' Ann Rehabil Med 37 (2):286‐90. doi: 10.5535/arm.2013.37.2.286. Korutla, L., J. Y. Cheung, J. Mendelsohn, and R. Kumar. 1995. 'Inhibition of ligand‐induced activation of epidermal growth factor receptor tyrosine phosphorylation by curcumin.' Carcinogenesis 16 (8):1741‐5. Kramer, A., I. Liashkovich, H. Oberleithner, S. Ludwig, I. Mazur, and V. Shahin. 2008. 'Apoptosis leads to a degradation of vital components of active nuclear transport and a dissociation of the nuclear lamina.' Proc Natl Acad Sci U S A 105 (32):11236‐41. doi: 10.1073/pnas.0801967105. Krug, R. M., W. Yuan, D. L. Noah, and A. G. Latham. 2003. 'Intracellular warfare between human influenza viruses and human cells: the roles of the viral NS1 protein.' Virology 309 (2):181‐9. Kumar, N., Z. T. Xin, Y. Liang, H. Ly, and Y. Liang. 2008. 'NF‐kappaB signaling differentially regulates influenza virus RNA synthesis.' J Virol 82 (20):9880‐9. doi: 10.1128/JVI.00909‐08. Kutluay, S. B., J. Doroghazi, M. E. Roemer, and S. J. Triezenberg. 2008. 'Curcumin inhibits herpes simplex virus immediate‐early gene expression by a mechanism independent of p300/CBP histone acetyltransferase activity.' Virology 373 (2):239‐47. doi: 10.1016/j.virol.2007.11.028. Lamb, R. A., and M. Takeda. 2001. 'Death by influenza virus protein.' Nat Med 7 (12):1286‐8. doi: 10.1038/nm1201‐1286. Le, Q. M., M. Kiso, K. Someya, Y. T. Sakai, T. H. Nguyen, K. H. Nguyen, N. D. Pham, H. H. Ngyen, S. Yamada, Y. Muramoto, T. Horimoto, A. Takada, H. Goto, T. Suzuki, Y. Suzuki, and Y. Kawaoka. 2005. 'Avian flu: isolation of drug‐resistant H5N1 virus.' Nature 437 (7062):1108. doi: 10.1038/4371108a. Lee, J., Y. H. Im, H. H. Jung, J. H. Kim, J. O. Park, K. Kim, W. S. Kim, J. S. Ahn, C. W. Jung, Y. S. Park, W. K. Kang, and K. Park. 2005. 'Curcumin inhibits interferon‐alpha induced NF‐kappaB and COX‐2 in human A549 non‐small cell lung cancer cells.' Biochem Biophys Res Commun 334 (2):313‐8. doi: 10.1016/j.bbrc.2005.06.093. Li, C. J., L. J. Zhang, B. J. Dezube, C. S. Crumpacker, and A. B. Pardee. 1993. 'Three inhibitors of type 1 human immunodeficiency virus long terminal repeat‐directed gene expression and virus replication.' Proc Natl Acad Sci U S A 90 (5):1839‐42. Lin, J. K., M. H. Pan, and S. Y. Lin‐Shiau. 2000. 'Recent studies on the biofunctions and biotransformations of curcumin.' Biofactors 13 (1‐4):153‐8. Loregian, A., B. Mercorelli, G. Nannetti, C. Compagnin, and G. Palu. 2014. 'Antiviral strategies against influenza virus: towards new therapeutic approaches.' Cell Mol Life Sci. doi: 10.1007/s00018‐014‐1615‐2. Lu, Y., M. Wambach, M. G. Katze, and R. M. Krug. 1995. 'Binding of the influenza virus NS1 protein to double‐stranded RNA inhibits the activation of the protein kinase that phosphorylates the elF‐2 translation initiation factor.' Virology 214 (1):222‐8. Ludwig, S., T. Wolff, C. Ehrhardt, W. J. Wurzer, J. Reinhardt, O. Planz, and S. Pleschka. 2004. 'MEK inhibition impairs influenza B virus propagation without emergence of resistant variants.' FEBS Lett 561 (1‐3):37‐43. doi: 10.1016/S0014‐5793(04)00108‐5. Lundbaek, J. A. 2008. 'Lipid bilayer‐mediated regulation of ion channel function by amphiphilic drugs.' J Gen Physiol 131 (5):421‐9. doi: 10.1085/jgp.200709948. Lyall, J., R. M. Irvine, A. Sherman, T. J. McKinley, A. Nunez, A. Purdie, L. Outtrim, I. H. Brown, G. Rolleston‐Smith, H. Sang, and L. Tiley. 2011. 'Suppression of avian influenza transmission in genetically modified chickens.' Science 331 (6014):223‐6. doi: 10.1126/science.1198020. Mahmoudian, S., S. Auerochs, M. Grone, and M. Marschall. 2009. 'Influenza A virus proteins PB1 and NS1 are subject to functionally important phosphorylation by protein kinase C.' J Gen Virol 90 (Pt 6):1392‐7. doi: 10.1099/vir.0.009050‐0. Mazur, I., W. J. Wurzer, C. Ehrhardt, S. Pleschka, P. Puthavathana, T. Silberzahn, T. Wolff,O. Planz, and S. Ludwig. 2007. 'Acetylsalicylic acid (ASA) blocks influenza virus propagation via its NF‐kappaB‐inhibiting activity.' Cell Microbiol 9 (7):1683‐94. doi: 10.1111/j.1462‐5822.2007.00902.x. Meier, V., E. Burger, S. Mihm, B. Saile, and G. Ramadori. 2003. 'Ribavirin inhibits DNA, RNA, and protein synthesis in PHA‐stimulated human peripheral blood mononuclear cells: possible explanation for therapeutic efficacy in patients with chronic HCV infection.' J Med Virol 69 (1):50‐8. doi: 10.1002/jmv.10264. Mizushina, Y., T. Ishidoh, T. Takeuchi, N. Shimazaki, O. Koiwai, K. Kuramochi, S. Kobayashi, F. Sugawara, K. Sakaguchi, and H. Yoshida. 2005. 'Monoacetylcurcumin: a new inhibitor of eukaryotic DNA polymerase lambda and a new ligand for inhibitor‐affinity chromatography.' Biochem Biophys Res Commun 337 (4):1288‐95. doi: 10.1016/j.bbrc.2005.10.005. Mizushina, Y., S. Kamisuki, N. Kasai, T. Ishidoh, N. Shimazaki, M. Takemura, H. Asahara, S. Linn, S. Yoshida, O. Koiwai, F. Sugawara, H. Yoshida, and K. Sakaguchi. 2002. 'Petasiphenol: a DNA polymerase lambda inhibitor.' Biochemistry 41 (49):14463‐71. Morita, M., K. Kuba, A. Ichikawa, M. Nakayama, J. Katahira, R. Iwamoto, T. Watanebe, S. Sakabe, T. Daidoji, S. Nakamura, A. Kadowaki, T. Ohto, H. Nakanishi, R. Taguchi, T. Nakaya, M. Murakami, Y. Yoneda, H. Arai, Y. Kawaoka, J. M. Penninger, M. Arita, and Y. Imai. 2013. 'The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza.' Cell 153 (1):112‐25. doi: 10.1016/j.cell.2013.02.027. Musiol, A., S. Gran, C. Ehrhardt, S. Ludwig, T. Grewal, V. Gerke, and U. Rescher. 2013. 'Annexin A6‐balanced late endosomal cholesterol controls influenza a replication and propagation.' MBio 4 (6):e00608‐13. doi: 10.1128/mBio.00608‐13. Nagata, K., A. Kawaguchi, and T. Naito. 2008. 'Host factors for replication and transcription of the influenza virus genome.' Rev Med Virol 18 (4):247‐60. doi: 10.1002/rmv.575. Nayak, D. P., E. K. Hui, and S. Barman. 2004. 'Assembly and budding of influenza virus.' Virus Res 106 (2):147‐65. doi: 10.1016/j.virusres.2004.08.012. Nelson, M. I., and E. C. Holmes. 2007. 'The evolution of epidemic influenza.' Nat Rev Genet 8 (3):196‐205. doi: 10.1038/nrg2053. Neumann, G., T. Noda, and Y. Kawaoka. 2009. 'Emergence and pandemic potential of swine‐origin H1N1 influenza virus.' Nature 459 (7249):931‐9. doi: 10.1038/nature08157. Nicholls, H. 2006. 'Pandemic influenza: the inside story.' PLoS Biol 4 (2):e50. doi:10.1371/journal.pbio.0040050. Nichols, T. C. 2004. 'NF‐kappaB and reperfusion injury.' Drug News Perspect 17 (2):99‐104. Nimmerjahn, F., D. Dudziak, U. Dirmeier, G. Hobom, A. Riedel, M. Schlee, L. M. Staudt, A. Rosenwald, U. Behrends, G. W. Bornkamm, and J. Mautner. 2004. 'Active NF‐kappaB signalling is a prerequisite for influenza virus infection.' J Gen Virol 85 (Pt 8):2347‐56. doi: 10.1099/vir.0.79958‐0. Nishida, M., S. Nishiumi, Y. Mizushina, Y. Fujishima, K. Yamamoto, A. Masuda, S. Mizuno, T. Fujita, Y. Morita, H. Kutsumi, H. Yoshida, T. Azuma, and M. Yoshida. 2010. 'Monoacetylcurcumin strongly regulates inflammatory responses through inhibition of NF‐kappaB activation.' Int J Mol Med 25 (5):761‐7. O'Neill, R. E., J. Talon, and P. Palese. 1998. 'The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins.' EMBO J 17 (1):288‐96. doi: 10.1093/emboj/17.1.288. Ohuchi, M., N. Asaoka, T. Sakai, and R. Ohuchi. 2006. 'Roles of neuraminidase in the initial stage of influenza virus infection.' Microbes Infect 8 (5):1287‐93. doi: 10.1016/j.micinf.2005.12.008. Ou, J. L., Y. Mizushina, S. Y. Wang, D. Y. Chuang, M. Nadar, and W. L. Hsu. 2013. 'Structure‐activity relationship analysis of curcumin analogues on anti‐influenza virus activity.' FEBS J. doi: 10.1111/febs.12503. Ozawa, M., K. Fujii, Y. Muramoto, S. Yamada, S. Yamayoshi, A. Takada, H. Goto, T. Horimoto, and Y. Kawaoka. 2007. 'Contributions of two nuclear localization signals of influenza A virus nucleoprotein to viral replication.' J Virol 81 (1):30‐41. doi: 10.1128/JVI.01434‐06. Pahl, H. L. 1999. 'Activators and target genes of Rel/NF‐kappaB transcription factors.' Oncogene 18 (49):6853‐66. doi: 10.1038/sj.onc.1203239. Pauli, E. K., M. Schmolke, T. Wolff, D. Viemann, J. Roth, J. G. Bode, and S. Ludwig. 2008. 'Influenza A virus inhibits type I IFN signaling via NF‐kappaB‐dependent induction of SOCS‐3 expression.' PLoS Pathog 4 (11):e1000196. doi: 10.1371/journal.ppat.1000196. Pinto, L. H., and R. A. Lamb. 2006. 'The M2 proton channels of influenza A and B viruses.' J Biol Chem 281 (14):8997‐9000. doi: 10.1074/jbc.R500020200. Planz, O. 2013. 'Development of cellular signaling pathway inhibitors as new antivirals against influenza.' Antiviral Res 98 (3):457‐68. doi: 10.1016/j.antiviral.2013.04.008. Ravindranath, V., and N. Chandrasekhara. 1981. 'Metabolism of curcumin‐‐studies with [3H]curcumin.' Toxicology 22 (4):337‐44. Rothberg, M. B., S. D. Haessler, and R. B. Brown. 2008. 'Complications of viral influenza.' Am J Med 121 (4):258‐64. doi: 10.1016/j.amjmed.2007.10.040. Rudneva, I. A., V. P. Kovaleva, N. L. Varich, V. R. Farashyan, L. V. Gubareva, S. S. Yamnikova, I. A. Popova, V. P. Presnova, and N. V. Kaverin. 1993. 'Influenza A virus reassortants with surface glycoprotein genes of the avian parent viruses: effects of HA and NA gene combinations on virus aggregation.' Arch Virol 133 (3‐4):437‐50. Saladino, R., M. Barontini, M. Crucianelli, L. Nencioni, R. Sgarbanti, and A. T. Palamara. 2010. 'Current advances in anti‐influenza therapy.' Curr Med Chem 17 (20):2101‐40. Sandur, S. K., M. K. Pandey, B. Sung, K. S. Ahn, A. Murakami, G. Sethi, P. Limtrakul, V. Badmaev, and B. B. Aggarwal. 2007. 'Curcumin, demethoxycurcumin, bisdemethoxycurcumin, tetrahydrocurcumin and turmerones differentially regulate anti‐inflammatory and anti‐proliferative responses through a ROS‐independent mechanism.' Carcinogenesis 28 (8):1765‐73. doi: 10.1093/carcin/bgm123. Shin, Y. K., Q. Liu, S. K. Tikoo, L. A. Babiuk, and Y. Zhou. 2007. 'Effect of the phosphatidylinositol 3‐kinase/Akt pathway on influenza A virus propagation.' J Gen Virol 88 (Pt 3):942‐50. doi: 10.1099/vir.0.82483‐0. Si, X., Y. Wang, J. Wong, J. Zhang, B. M. McManus, and H. Luo. 2007. 'Dysregulation of the ubiquitin‐proteasome system by curcumin suppresses coxsackievirus B3 replication.' J Virol 81 (7):3142‐50. doi: 10.1128/JVI.02028‐06. Singh, S., and A. Khar. 2006. 'Biological effects of curcumin and its role in cancer chemoprevention and therapy.' Anticancer Agents Med Chem 6 (3):259‐70. Su, C. Y., T. J. Cheng, M. I. Lin, S. Y. Wang, W. I. Huang, S. Y. Lin‐Chu, Y. H. Chen, C. Y. Wu, M. M. Lai, W. C. Cheng, Y. T. Wu, M. D. Tsai, Y. S. Cheng, and C. H. Wong. 2010. 'High‐throughput identification of compounds targeting influenza RNA‐dependent RNA polymerase activity.' Proc Natl Acad Sci U S A 107 (45):19151‐6. doi: 10.1073/pnas.1013592107. Taubenberger, J. K., and D. M. Morens. 2006. '1918 Influenza: the mother of all pandemics.' Emerg Infect Dis 12 (1):15‐22. doi: 10.3201/eid1201.050979. Taubenberger, J. K., A. H. Reid, A. E. Krafft, K. E. Bijwaard, and T. G. Fanning. 1997. 'Initial genetic characterization of the 1918 'Spanish' influenza virus.' Science 275 (5307):1793‐6. Tong, S., X. Zhu, Y. Li, M. Shi, J. Zhang, M. Bourgeois, H. Yang, X. Chen, S. Recuenco, J. Gomez, L. M. Chen, A. Johnson, Y. Tao, C. Dreyfus, W. Yu, R. McBride, P. J. Carney, A. T. Gilbert, J. Chang, Z. Guo, C. T. Davis, J. C. Paulson, J. Stevens, C. E.Rupprecht, E. C. Holmes, I. A. Wilson, and R. O. Donis. 2013. 'New world bats harbor diverse influenza A viruses.' PLoS Pathog 9 (10):e1003657. doi: 10.1371/journal.ppat.1003657. Triana‐Baltzer, G. B., L. V. Gubareva, A. I. Klimov, D. F. Wurtman, R. B. Moss, M. Hedlund, J. L. Larson, R. B. Belshe, and F. Fang. 2009. 'Inhibition of neuraminidase inhibitor‐resistant influenza virus by DAS181, a novel sialidase fusion protein.' PLoS One 4 (11):e7838. doi: 10.1371/journal.pone.0007838. Trifonov, V., H. Khiabanian, and R. Rabadan. 2009. 'Geographic dependence, surveillance, and origins of the 2009 influenza A (H1N1) virus.' N Engl J Med 361 (2):115‐9. doi: 10.1056/NEJMp0904572. Vajragupta, O., P. Boonchoong, G. M. Morris, and A. J. Olson. 2005. 'Active site binding modes of curcumin in HIV‐1 protease and integrase.' Bioorg Med Chem Lett 15 (14):3364‐8. doi: 10.1016/j.bmcl.2005.05.032. Vanhaesebroeck, B., K. Ali, A. Bilancio, B. Geering, and L. C. Foukas. 2005. 'Signalling by PI3K isoforms: insights from gene‐targeted mice.' Trends Biochem Sci 30 (4):194‐204. doi: 10.1016/j.tibs.2005.02.008. von Itzstein, M. 2007. 'The war against influenza: discovery and development of sialidase inhibitors.' Nat Rev Drug Discov 6 (12):967‐74. doi: 10.1038/nrd2400. Writing Committee of the, W. H. O. Consultation on Clinical Aspects of Pandemic Influenza, E. Bautista, T. Chotpitayasunondh, Z. Gao, S. A. Harper, M. Shaw, T. M. Uyeki, S. R. Zaki, F. G. Hayden, D. S. Hui, J. D. Kettner, A. Kumar, M. Lim, N. Shindo, C. Penn, and K. G. Nicholson. 2010. 'Clinical aspects of pandemic 2009 influenza A (H1N1) virus infection.' N Engl J Med 362 (18):1708‐19. doi: 10.1056/NEJMra1000449. Wurzer, W. J., C. Ehrhardt, S. Pleschka, F. Berberich‐Siebelt, T. Wolff, H. Walczak, O. Planz, and S. Ludwig. 2004. 'NF‐kappaB‐dependent induction of tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) and Fas/FasL is crucial for efficient influenza virus propagation.' J Biol Chem 279 (30):30931‐7. doi: 10.1074/jbc.M403258200. Xiang, L., Y. Nakamura, Y. M. Lim, Y. Yamasaki, Y. Kurokawa‐Nose, W. Maruyama, T. Osawa, A. Matsuura, N. Motoyama, and L. Tsuda. 2011. 'Tetrahydrocurcumin extends life span and inhibits the oxidative stress response by regulating the FOXO forkhead transcription factor.' Aging (Albany NY) 3 (11):1098‐109. Yamada, H., R. Chounan, Y. Higashi, N. Kurihara, and H. Kido. 2004. 'Mitochondrial targeting sequence of the influenza A virus PB1‐F2 protein and its function in mitochondria.' FEBS Lett 578 (3):331‐6. doi: 10.1016/j.febslet.2004.11.017. Zhang, L., P. Das, M. Schmolke, B. Manicassamy, Y. Wang, X. Deng, L. Cai, B. P. Tu, C. V.Forst, M. G. Roth, D. E. Levy, A. Garcia‐Sastre, J. de Brabander, M. A. Phillips, and B. M. Fontoura. 2012. 'Inhibition of pyrimidine synthesis reverses viral virulence factor‐mediated block of mRNA nuclear export.' J Cell Biol 196 (3):315‐26. doi: 10.1083/jcb.201107058. Zhu, L., Y. Li, S. Li, H. Li, Z. Qiu, C. Lee, H. Lu, X. Lin, R. Zhao, L. Chen, J. Z. Wu, G. Tang, and W. Yang. 2011. 'Inhibition of influenza A virus (H1N1) fusion by benzenesulfonamide derivatives targeting viral hemagglutinin.' PLoS One 6 (12):e29120. doi: 10.1371/journal.pone.0029120.
摘要: Curcumin (Cur) is a commonly used coloring agent and spice in food. Previously, we reported curcumin blocks influenza A virus (IAV) activity via multiple mechanisms. By comparing the effect of curcumin analogues on IAV infection, monoacetylcurcumin (MAC) harbors a similar anti-IAV strength to curcumin. However, unlike curcumin, MAC did not inhibit viral HA activity, indicating that MAC possibly blocks IAV via a different mechanism. Hence, in the study herein, effect of these two compounds on several aspects related to IAV infection was compared. The results showed that as with curcumin, MAC attacked membrane structure in the liposome system. In NA inhibition assay, curcumin and MAC interfered with the activity of NA. Curcumin and MAC have been reported to inhibit Akt and NF-B pathways, two of cellular signaling pathways activated by IAV and are required for IAV replication. Treatment of curcumin analogues after virus entry indicated that curcumin and MAC inhibited phosphorylation (activation) of Akt and NF-B to a similar extent. Results of hemolysis inhibition assay and plaque reduction assay indicated curcumin and MAC inhibited membrane fusion activity. Finally, results of mini-replicon assay showed that treatment of curcumin or MAC inhibited viral RNA translation and transcription. Taken together, as a strong inhibitor of IAV, MAC shares similar effect to curcumin, but it does not inhibit HA activity.
薑黃素(Curcumin)為薑黃(Curcuma longa)之萃取物,可做為食物香料使用,目前研究已知curcumin 具有抗腫瘤、抗發炎、抗氧化、增強免疫力以及預防阿茲海默症的能力。實驗室先前研究發現curcumin 會透過干擾HA 的功能達到抑制流感病毒活性, 比較分析curcumin 結構類似物的抗流感病毒能力顯示:monoacetylcurcumin (MAC)具有與curcumin 不相上下的抗流感病毒能力,但是MAC 並不抑制流感病毒蛋白HA 的功能,而且在病毒感染後給予藥物依舊能強烈地抑制流感病毒增殖,顯現curcumin 除了抑制病毒蛋白HA 外還有其他抗流感機制,本研究透過一系列的實驗分析比較curcumin 與MAC 的抗流感機轉。以微脂體(liposome)模擬病毒實驗得知,curcumin 與MAC 均具有破壞微脂體的能力,此外,NA 封套蛋白的功能也會受curcumin 與MAC 所干擾。已知curcumin 與MAC可以抑制細胞訊息蛋白Akt、NF-b 的活化,而流感病毒感染時會藉由活化這兩條細胞訊息路徑來提高感染效率;本研究透過西方墨點法來觀測curcumin 與MAC抑制流感病毒感染活化Akt 與NF-b 的能力,結果證實這兩者藥物明顯抑制細胞中因為流感病毒感染而磷酸化的Akt 與NF-b 蛋白量。透過溶血抑制分析以及不同感染時間點進行藥物處理的方法來研究curcumin 與MAC 對於病毒與細胞融合的影響,結果證實處理藥物可以降低病毒與細胞融合,而使流感病毒無法進行後續的感染。另藉由mini-replicon assay 得知curcumin 與MAC均會干擾流感病毒RNA在細胞中的轉錄及轉譯。綜上所述,curcumin 與MAC 有相近的抗流感病毒能力,能透過不同的抗流感機制達到抑制流感病毒感染的目的。
文章公開時間: 2018-07-16
Appears in Collections:微生物暨公共衛生學研究所



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