Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/93085
標題: In Vitro analysis of the anti-influenza virus activity of Peperomia sui and Ixeris chinensis
紅莖椒草與兔兒菜萃取物抗流感病毒功能之分析
作者: 楊志學
Chih-Hsueh Yang
關鍵字: 禽流感
紅莖椒草
兔兒菜
抗病毒活性
血球凝集反應
Influenza virus
Peperomia sui
Ixeris chinensis
Antiviral activity
Hemagglutination
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摘要: Influenza is a respiratory infection. It causes high mortality of annual epidemics and occasional pandemics in the past. Recently, Many new types of mutated influenza viruses have occurred. The outbreak of H5N1 avian flu in 2003 and the new type H1N1 human flu in 2009 have increased public vigilance of the potential for global influenza pandemics. Due to the emergence of neuraminidase inhibitor and M2 ion channel inhibitor resistant viruses, such as H5N1 and swine-origin H1N1 influenza viruses. Hence, new approaches for the control of infection with influenza viruses must be explored. In many reports, traditional Chinese medicinal herbs are considered a potential alternative medicine of choice for treatment of this disease. Peperomia sui Lin & Lu (P. sui), a well-known Taiwanese folk medicine, has a broad range of biological effects, especially in treatment of upper respiratory tract diseases. In addition, Ixeris chinensis (Thunb.) Nakai (I. chinensis), has been used to prepare a traditional Chinese medicine composition for prevention and treatment of influenza A viral infection. Until now the effects of P. sui and I. chinensis on the influenza virus have not been examined. In this study, the in vitro anti-influenza viral effect of P. sui and I. chinensis were evaluated. The 50% ethanol extract from P. sui and I. chinensis significantly increased the viability of in chicken fibroblast DF-1 cells that were infected by the A/Chicken/TW/0518/2011(H6N1) virus. This extract also suppressed the synthesis of viral nucleoprotein (NP), and inhibited the propagation of the virus in DF-1 cells. Further, the 50% ethanol extract of P. sui and I. chinensis effectively inhibited the neuraminidase activity of H6N1 virus. Overall, these findings provide important information for the exploitation and utilization of P.sui and I. chinensis in prevention and treatment of influenza infection.
流感屬於呼吸道感染疾病,過去曾爆發多起世界性的大流行。近年來,也爆發多次對人類威脅性大的流感,如2003年的禽流感H5N1和2009年的新型流感H1N1。因為流感病毒是不斷的突變,隨時會有大流行的可能性,且近來臨床報導指出,抗藥性的流感病毒株已大量出現,如豬流感病毒已被檢測出對M2抑制劑的抗藥性,而香港爆發的禽流感則對克流感具有抗藥性。因此,開發新的安全有效之預防或治療流感或抗流感病毒之藥物,是傳染病醫學界刻不容緩的重要課題。傳統中草藥就常應用於呼吸道疾病或病毒感染傳染病,故本論文主要是以細胞培養模式,由傳統中草藥中找出可抑制流感病毒的藥物,以應對日益嚴重的流感病毒突變與藥物抗藥性問題。紅莖椒草在台灣民間傳統廣泛的使用於多種疾病,尤其是用於治療上呼吸道感染疾病;兔兒菜在傳統中醫也應用於預防與治療流感。但這兩種植物藥抗流感病毒之功效,至今尚無研究證實。因此,本研究為評估紅莖椒草與兔兒菜體外抗流感病毒之效果,將紅莖椒草Peperomia sui Lin & Lu (P. sui)與兔兒菜Ixeris chinensis (Thunb.) Nakai (I. chinensis)之萃取物處理經A/Chicken/TW/0518/2011(H6N1) 病毒感染之雞成纖維細胞株(DF-1)後利用存活分析、血液凝集反應測定、神經胺酸酶活性測定分析、間接免疫螢光測定和定量RT-PCR等分析技術,測定其抗流感病毒H6N1之活性。結果發現紅莖椒草與兔兒菜乙醇萃取物可顯著改善因H6N1病毒感染造成DF-1細胞的死亡現象。紅莖椒草與兔兒菜乙醇萃取物也可抑制病毒核蛋白(Nucleoprotein,NP)的合成,並抑制病毒在DF-1細胞的複製。此外,紅莖椒草與兔兒菜乙醇萃取物也具有抑制H6N1病毒之神經胺酸酶的活性。此研究結果發現,紅莖椒草與兔兒菜具有抗流感病毒之能力及潛力,同時也提供紅莖椒草與兔兒菜日後開發為預防或治療流感病毒感染藥物的重要科學實證參考數據。
URI: http://hdl.handle.net/11455/93085
文章公開時間: 2018-06-29
Appears in Collections:微生物暨公共衛生學研究所

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