Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/93096
標題: The Haemagglutinin of Avibacterium paragallinarum is a Trimeric Adhesin that Confers Cell Adherence and Biofilm Formation Activities
雞傳染性鼻炎菌之血球凝集素為一具有吸附細胞與形成生物膜活性之三聚體吸附素
作者: 王宜苹
Yi-Ping Wang
關鍵字: 
no
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摘要: The haemagglutinin (HA) protein plays a key role in the immunogenicity and pathogenicity of Av. paragallinarum. In previous studies, a 210 kDa protein (HMTp210) was reported to be the HA of Av. paragallinarum but the biological function of this HMTp210 is not well-defined. In this study, mutant strains that lacked the HMTp210 protein were constructed by using the Targetron gene knockout system. Haemagglutination and haemagglutination-inhibition (HI) assays showed that the HMTp210-deficient mutants completely lost their haemagglutination activity and failed to elicit HI antibodies in immunized chickens. Additionally, the HMTp210-deficient mutants had a reduced ability to adhere to HeLa cells and form biofilms on abiotic surfaces. These results indicated that the HMTp210 protein is the major haemagglutination antigen of Av. paragallinarum and this protein is a trimeric autotransporter adhesin that confers cell adherence and biofilm formation activities. These results could be valuable for understanding the function of the HA and mechanism of pathogenicity of Av. paragallinarum.
血球凝集蛋白 (haemagglutinin; HA) 在雞傳染性鼻炎菌 (Avibacterium paragallinarum; Av. paragallinarum) 之免疫原性及致病力占有重要地位。過去研究發現 Av. paragallinarum 具有一分子量為 210 kDa 之 HMTp210 蛋白,為 Av. paragallinarum 之主要血球凝集蛋白,然而此 HMTp210 蛋白功能尚未明暸。本研究藉商業化套組 Targetron gene knockout system 構築此 210 kDa HMTp210 蛋白突變株。血球凝集試驗及血球凝集抑制試驗顯示 HMTp210 突變株完全喪失血球凝集能力,亦無法誘使雞隻產生抑制血球凝集抗體。此外,HMTp210 突變株黏附HeLa 細胞及產生生物膜的能力亦下降。上述研究結果顯示此 210 kDa HMTp210蛋白為 Av. paragallinarum 主要血球凝集抗原且屬於三聚體自體運輸黏附蛋白,其功能會影響細菌黏附細胞及產生生物膜之能力。本研究結果可增進對 Av. paragallinarum 致病機轉及其血球凝集蛋白功能之暸解。
URI: http://hdl.handle.net/11455/93096
文章公開時間: 2018-07-16
Appears in Collections:微生物暨公共衛生學研究所

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