Please use this identifier to cite or link to this item:
標題: The Haemagglutinin of Avibacterium paragallinarum is a Trimeric Adhesin that Confers Cell Adherence and Biofilm Formation Activities
作者: 王宜苹
Yi-Ping Wang
引用: 林茂勇、柯冠銘。可利查 (Infectious coryza)。引自:禽病診治,第二版。藝軒圖書出版社,臺北,177-180,2006。 Blackall PJ. Infectious coryza: overview of the disease and new diagnostic options. Clin Microbiol Rev 12: 627-632, 1999. Blackall PJ, Eaves LE, Aus G. Serotyping of Haemophilus paragallinarum by the Page scheme: comparison of the use of agglutination and hemagglutination-inhibition tests. Avian Dis 34: 643-645, 1990. Blackall PJ, Soriano EJ. Infectious coryza and related bacterial infections. In: Saif YM, Fadly AM, Glisson JR, Mcdougald LR, Nolan LK, Swayne DE, ed. Diseases of poultry. 12th ed. Blackwell, Australia, 789-803, 2011. Calderon EN, Thomas K, Morales-Erasto V, Salgado-Miranda C, Soriano-Vargas E. Identification of Avibacterium paragallinarum serovar B-1 from severe infectious coryza outbreaks in Panama. Avian Dis 54: 1095-1097, 2010. Chen X, Miflin JK, Zhang P, Blackall PJ. Development and application of DNA probes and PCR tests for Haemophilus paragallinarum. Avian Dis 40: 398-407, 1996. Chen YC, Tan DH, Shien JH, Hsieh MK, Yen TY, Chang PC. Identification and functional analysis of the cytolethal distending toxin gene from Avibacterium paragallinarum. Avian Pathol 43: 43-50, 2014. Chiang YT, Shien JH, Tan DH, Shieh MK, Liu CC, Chen YS, Chang PC. Identification of the lic1ABCD operon that controls the phase-variable expression of phosphorylcholine on lipopolysaccharide from Avibacterium paragallinarum. Avian Pathol 42: 72-78, 2013. Cotter SE, Surana NK, St Geme JW, 3rd. Trimeric autotransporters: a distinct subfamily of autotransporter proteins. Trends Microbiol 13: 199-205, 2005. El Tahir Y, Skurnik M. YadA, the multifaceted Yersinia adhesin. Int J Med Microbiol 291: 209-218, 2001. Fu XD. RNA Splicing and Processing. In: Krebs JE, Kilpatrick ST, Goldstein ES, ed. Lewin's Genes X. Jones and Bartlett Publishers, Massachusetts, 573-617, 2011. Hobb RI, Tseng HJ, Downes JE, Terry TD, Blackall PJ, Takagi M, Jennings MP. Molecular analysis of a haemagglutinin of Haemophilus paragallinarum. Microbiology 148: 2171-2179, 2002. Hsu YM, Shieh HK, Chen WH, Shiang JH, Chang PC. Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA. Vet Microbiol 122: 280-289, 2007. Jin H, Zhou R, Kang M, Luo R, Cai X, Chen H. Biofilm formation by field isolates and reference strains of Haemophilus parasuis. Vet Microbiol 118: 117-123, 2006. Kung E, Frey J. AvxA, a composite serine-protease-RTX toxin of Avibacterium paragallinarum. Vet Microbiol 163: 290-298, 2013. Lambowitz AM, Zimmerly S. Mobile group II introns. Annu Rev Genet 38: 1-35, 2004. Letourneau J, Levesque C, Berthiaume F, Jacques M, Mourez M. In vitro assay of bacterial adhesion onto mammalian epithelial cells. J Vis Exp 2011. Lyskowski A, Leo JC, Goldman A. Structure and Biology of Trimeric Autotransporter Adhesins. In: Linke D, Goldman A, ed. Bacterial Adhesion: Chemistry, Biology and Physics. Springer Science+Business Media B. V., 143-158, 2011. Noro T, Oishi E, Kaneshige T, Yaguchi K, Amimoto K, Shimizu M. Identification and characterization of haemagglutinin epitopes of Avibacterium paragallinarum serovar C. Vet Microbiol 131: 406-413, 2008. Noro T, Yaguchi K, Amimoto K, Oishi E. Identification and expression of a gene encoding an epitope that induces haemagglutination inhibition antibody to Avibacterium paragallinarum serovar A. Avian Dis 51: 84-89, 2007. Percival SL, Malic S, Cruz H, Williams DW. Introduction to Biofilms. In: Percival SL, Knottenbelt DC, Cochrane CA, ed. Biofilms and Veterinary Medicine. Springer, Berlin, Heidelberg, 41-68, 2011. Pizarro-Cerda J, Cossart P. Bacterial adhesion and entry into host cells. Cell 124: 715- 727, 2006. Quinn PJ, Markey BK, Leonard FC, Fitzpatrick ES, Fanning S, Hartigan PJ. Histophilus somni, Haemophilus parasuis and Avibacterium paragallinarum. In: Veterinary Microbiology and Microbial Disease. 2nd ed. Wiley-Blackwell, Oxford, 314-320.2011. Sakamoto R, Baba S, Ushijima T, Kino Y, Honda T, Mizokami H, Sakaguchi M. Development of a recombinant vaccine against infectious coryza in chickens. Res Vet Sci 94: 504-509, 2013. Sandal I, Corbeil LB, Inzana TJ. Haemophilus. In: Gyles CL, Prescott JF, Songer G, Thoen CO, ed. Pathogenesis of bacterial infections in animal. 4th ed. Wiley- Blackwell, Iowa, 2010. Schembri MA, Dalsgaard D, Klemm P. Capsule shields the function of short bacterial adhesins. J Bacteriol 186: 1249-1257, 2004. Serra DO, Conover MS, Arnal L, Sloan GP, Rodriguez ME, Yantorno OM, Deora R. FHA-mediated cell-substrate and cell-cell adhesions are critical for Bordetella pertussis biofilm formation on abiotic surfaces and in the mouse nose and the trachea. PLoS One 6: e28811, 2011. St Geme JW, 3rd, Cutter D. Influence of pili, fibrils, and capsule on in vitro adherence by Haemophilus influenzae type b. Mol Microbiol 21: 21-31, 1996. Thanassi DG. The long and the short of bacterial adhesion regulation. J Bacteriol 193: 327-328, 2011. Tokunaga E, Sakaguchi M, Matsuo K, Hamada F, Tokiyoshi S. Polypeptide for Haemophilus paragallinarum and process for preparing the same. United States Patent No. 6,919,080 B2, 2005. Toro N. Bacteria and Archaea Group II introns: additional mobile genetic elements in the environment. Environ Microbiol 5: 143-151, 2003. Wilson M, Mcnab R, Henderson B. Bacterial adhesion as a virulence mechanism. In: Bacterial disease mechanisms. Cambridge university press, Cambridge, 353-404, 2002. Wu JR, Shieh HK, Shien JH, Gong SR, Chang PC. Molecular characterization of plasmids with antimicrobial resistant genes in avian isolates of Pasteurella multocida. Avian Dis 47: 1384-1392, 2003. Wu JR, Chen PY, Shien JH, Shyu CL, Shieh HK, Chang F, Chang PC. Analysis of the biosynthesis genes and chemical components of the capsule of Avibacterium paragallinarum. Vet Microbiol 145: 90-99, 2010. Wu JR, Wu YR, Shien JH, Hsu YM, Chen CF, Shieh HK, Chang PC. Recombinant proteins containing the hypervariable region of the haemagglutinin protect chickens against challenge with Avibacterium paragallinarum. Vaccine 29: 660- 667, 2011. Yamaguchi T, Iritani Y, Hayashi Y. Hemagglutinating activity and immunological properties of Haemophilus paragallinarum field isolates in Japan. Avian Dis 33: 511-515, 1989. Yamaguchi T, Kobayashi M, Masaki S, Iritani Y. Isolation and characterization of a Haemophilus paragallinarum mutant that lacks a hemagglutinating antigen. Avian Dis 37: 970-976, 1993. Zhou Y, Li L, Chen Z, Yuan H, Chen H, Zhou R. Adhesion protein ApfA of Actinobacillus pleuropneumoniae is required for pathogenesis and is a potential target for vaccine development. Clin Vaccine Immunol 20: 287-294, 2013.
摘要: The haemagglutinin (HA) protein plays a key role in the immunogenicity and pathogenicity of Av. paragallinarum. In previous studies, a 210 kDa protein (HMTp210) was reported to be the HA of Av. paragallinarum but the biological function of this HMTp210 is not well-defined. In this study, mutant strains that lacked the HMTp210 protein were constructed by using the Targetron gene knockout system. Haemagglutination and haemagglutination-inhibition (HI) assays showed that the HMTp210-deficient mutants completely lost their haemagglutination activity and failed to elicit HI antibodies in immunized chickens. Additionally, the HMTp210-deficient mutants had a reduced ability to adhere to HeLa cells and form biofilms on abiotic surfaces. These results indicated that the HMTp210 protein is the major haemagglutination antigen of Av. paragallinarum and this protein is a trimeric autotransporter adhesin that confers cell adherence and biofilm formation activities. These results could be valuable for understanding the function of the HA and mechanism of pathogenicity of Av. paragallinarum.
血球凝集蛋白 (haemagglutinin; HA) 在雞傳染性鼻炎菌 (Avibacterium paragallinarum; Av. paragallinarum) 之免疫原性及致病力占有重要地位。過去研究發現 Av. paragallinarum 具有一分子量為 210 kDa 之 HMTp210 蛋白,為 Av. paragallinarum 之主要血球凝集蛋白,然而此 HMTp210 蛋白功能尚未明暸。本研究藉商業化套組 Targetron gene knockout system 構築此 210 kDa HMTp210 蛋白突變株。血球凝集試驗及血球凝集抑制試驗顯示 HMTp210 突變株完全喪失血球凝集能力,亦無法誘使雞隻產生抑制血球凝集抗體。此外,HMTp210 突變株黏附HeLa 細胞及產生生物膜的能力亦下降。上述研究結果顯示此 210 kDa HMTp210蛋白為 Av. paragallinarum 主要血球凝集抗原且屬於三聚體自體運輸黏附蛋白,其功能會影響細菌黏附細胞及產生生物膜之能力。本研究結果可增進對 Av. paragallinarum 致病機轉及其血球凝集蛋白功能之暸解。
文章公開時間: 2018-07-16
Appears in Collections:微生物暨公共衛生學研究所



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.