Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/93343
標題: 管柱流動層析結合超臨界二氧化碳抗溶沉澱杜莎藻富含類胡蘿蔔素粉粒體
Column Elution Chromatography Coupled with Supercritical Carbon Dioxide Antisolvent Precipitation of Carotenoids-rich Particulates from Dunaliella salina
作者: Yi-Chen Shen
沈宜臻
關鍵字: 杜莎藻
反式-b-胡蘿蔔素
9-順-b -胡蘿蔔素
管柱純化
超臨界二氧化碳抗溶沉澱
應答曲面實驗設計
微米級顆粒
Dunaliella salina
trans-b-carotene
9-cis-b -carotene
Column elution chromatography
Supercritical anti solvent precipitation
Response surface method design
micronized precipitates
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摘要: 本研究以反相樹脂管柱純化配合超臨界抗溶沉澱法自海水養殖杜莎藻粉製備富含類胡蘿蔔素的微米級粉粒體。首先,超音波攪拌乙醚或四氫呋喃萃取杜莎藻粉,可獲得每克杜莎藻藻粉含61.3毫克(乙醚)至72.5毫克(四氫呋喃)的總類胡蘿蔔素(即反式-b-胡蘿蔔素及9-順-b-胡蘿蔔素)。當6公升乙醇以管柱沖提方式純化2克乙醚粗萃物,可得固形物含624.9mg/g反式-b-胡蘿蔔素,回收率為84.6%;10公升乙醇以相同方式沖提同量的四氫呋喃粗萃物,可得固形物含823.6mg/g反式-b-胡蘿蔔素,回收率為86.2%。以超臨界抗溶近飽和管柱純化的乙醚溶液,反式-b-胡蘿蔔素純度無法提高,發現它較葉綠素易溶於高壓二氧化碳與乙醚混和溶液,而被帶出沉澱槽外。但以160bar及318K超臨界抗溶四氫呋喃溶液,可使反式-b-胡蘿蔔素純度提高至90%以上,且獲得粉狀沉澱物。最後,以應答曲面實驗設計的超臨界抗溶5mg/ml的四氫呋喃溶液,在進料流速0.2ml/min、二氧化碳流速15L/min,溫度318K時,改變壓力從140bar至180bar及時間從40分鐘至60分鐘,成功地將反式-b-胡蘿蔔素純度提升至926.8mg/g(92.7%),回收率54%,並獲得塊狀沉澱顆粒,其粒徑介於3.5至19微米之間。
This work investigated column elution chromatography of ultrasonic solvent extracts coupled with supercritical anti-solvent precipitation of carotenoids-containing particulates from microalgal Dunaliella Salina species. The extract contained carotenoids ranged from 61.3mg to 72.5mg using ultrasonic stirred ethyl ether or tetrahydrofuran (THF) extraction. When 6L ethyl alcohol was used in a reverse-phase column to elute 2g of the ethyl ether extract, the purity of trans-b-carotene attained 624.9mg/g, and the recovery was 84.6%. When 10L of ethyl alcohol employed for eluting the THF extract, the purity of trans-b-carotene increased to 823.6 mg/g with a recovery of 86.2%. Supercritical anti-solvent of ethyl ether solution did not enhance the purity of carotenoids, implied that carbon dioxide dissolves more carotenoids than chlorophylls into high-pressure solution. However, supercritical anti-solvent of THF solution at 160bar and 318K produced powdered particulates with purity of carotenoids above 90%. Subsequently, a center composited schemed response surface method designed supercritical anti-solvent precipitation of carotenoids-rich THF solution by changing pressure from 140bar to 160bar and time from 40min to 60min at feed flow rate of 0.2ml/min, CO2 flow rate of 15L/min and temperature of 318K was examined to find effects on the purity and recovery of trans-b-carotene. There experimental indeed produced micronized precipitates with mean particle size range from 3.5 micro meter to 19 micro meter and the purity of trans-b- carotene increased to 926.8mg/g with a recovery of 54%.
URI: http://hdl.handle.net/11455/93343
其他識別: U0005-2407201123485400
文章公開時間: 2016-07-28
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