Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/93819
標題: Azospirillum soli sp. nov., a nitrogen-fixing species isolated from agricultural soil
作者: Lin, Shih-Yao
Hameed, Asif
Liu, You-Cheng
Hsu, Yi-Han
Lai, Wei-An
Shen, Fo-Ting
Young, Chiu-Chung
關鍵字: Azospirillum
Bacterial Typing Techniques
Base Composition
DNA, Bacterial
Fatty Acids
Molecular Sequence Data
Nucleic Acid Hybridization
Phospholipids
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Taiwan
Ubiquinone
Nitrogen Fixation
Phylogeny
Soil Microbiology
摘要: An aerobic, Gram-stain-negative, rod or spiral-shaped diazotrophic bacterium (designated strain CC-LY788T), was isolated from agricultural soil in Taiwan. Strain CC-LY788T was able to grow at 25-40 °C, pH 6.0-8.0 and tolerated NaCl to 2.0% (w/v). Positive for nitrogen fixation with the activity recorded as 6.5 nmol ethylene h(-1). Strain CC-LY788T showed highest 16S rRNA gene sequence similarity to Azospirillum picis DSM 19922T (97.2%) and Azospirillum rugosum DSM 19657T (97.1%) and lower sequence similarities (<96.6%) to all other species of the genus Azospirillum. According to the DNA-DNA hybridization, the relatedness values of strain CC-LY788T with A. picis DSM 19922T and A. rugosum DSM 19657T were 51.1±5.5% and 46.8±2.1%, respectively. Strain CC-LY788T was positive for the rapid identification of the genus-specific primer set. The respiratory quinone system was ubiquinone (Q-10) and the DNAG+C content was 69.8 mol%. The major fatty acids found in strain CC-LY788T were C16 : 0, C18 : 1 2-OH, C14 : 0 3-OH/C16 : 1 iso I (summed feature 2), C16 : 1ω7c/C16 : 1ω6c (summed feature 3), C18 : 0 ante/C18 : 2ω6,9c (summed feature 5) and C18 : 1ω7c/C18 : 1ω6c (summed feature 8). Based on the phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY788T represents a novel species of the genus Azospirillum, for which the name Azospirillum soli sp. nov. is proposed. The type strain is CC-LY788T (=BCRC 80569T=JCM 18820T).
URI: http://hdl.handle.net/11455/93819
Appears in Collections:土壤環境科學系

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