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標題: Nocardioides echinoideorum sp. nov., isolated from sea urchins (Tripneustes gratilla)
作者: Lin, Shih-Yao
Wen, Cheng-Zhe
Hameed, Asif
Liu, You-Cheng
Hsu, Yi-Han
Shen, Fo-Ting
Lai, Wei-An
Young, Chiu-Chung
關鍵字: Actinomycetales
Bacteria, Aerobic
Bacterial Typing Techniques
Base Composition
DNA, Bacterial
Diaminopimelic Acid
Fatty Acids
Molecular Sequence Data
RNA, Ribosomal, 16S
Sea Urchins
Sequence Analysis, DNA
Vitamin K 2
摘要: A Gram-stain-positive, aerobic, non-motile, rod-shaped, yellow-pigment-producing bacterium, (designated strain CC-CZW004T), was isolated from seafood samples (sea urchins) at Penghu Island in Taiwan. Strain CC-CZW004T grew optimally at pH 7.0 and 30 °C in the presence of 3 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CZW004T with respect to other members of the genus Nocardioides. The novel strain shared highest 16S rRNA gene sequence similarities to Nocardioides daejeonensis JCM 16922T (96.4 %), Nocardioides pacificus JCM 19260T (96.3 %), and Marmoricola scoriae JCM 17444T (96.2 %). The major fatty acids of strain CC-CZW004T consisted of C17:0, C16:1ω5c, C17:1ω8c`, iso-C16:0 and C19:1ω11c/C19:1ω9c (summed feature 6). The diagnostic diamino acid in the cell wall was ll-2,6-diaminopimelic acid. The polar lipid profile was composed of major amounts of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 73.3 mol%. The predominant quinone system was menaquinone (MK-8). On the basis of polyphasic taxonomic evidences presented here, strain CC-CZW004T represents a novel species of the genus Nocardioides, for which the name Nocardioides echinoideorum sp. nov. is proposed. The type strain is CC-CZW004T ( = BCRC 16974T = JCM 30276T).
Appears in Collections:土壤環境科學系



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