Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/94131
標題: The citrus postharvest pathogen Penicillium digitatum depends on the PdMpkB kinase for developmental and virulence functions
作者: Ma, Haijie
Sun, Xuepeng
Wang, Mingshuang
Gai, Yunpeng
Chung, Kuang-Ren
Li, Hongye
鍾光仁
關鍵字: Fruit decay
Green mold
Orange
Osmotic
Pathogenicity
Citrus
Fungal Proteins
Gene Expression Profiling
Gene Expression Regulation, Fungal
Mitogen-Activated Protein Kinases
Osmotic Pressure
Penicillium
Plant Diseases
Spores, Fungal
Transcriptome
Virulence
摘要: The postharvest pathogen Penicillium digitatum causes green mold decay on citrus fruit, resulting in severe economic losses. To explore possible factors involved in fungal pathogenesis, phenotypic characterization of the budding yeast Fus3/Kiss1 mitogen-activated protein (MAP) kinase homolog was carried out. The P. digitatum MAP kinase B coding gene, designated PdMpkB, was functionally inactivated via homologous recombination. The fungal strain (∆PdMpkB) carrying a PdMpkBdeletion demonstrated altered gene expression profiles, reduced growth and conidiogenesis, elevated resistance to osmotic stress, and failed to induce green mold decay on citrus fruit. ∆PdMpkB was more resistant to CaCl2, NaCl and sorbitol than its progenitor strain, indicating a negative regulatory function of PdMpkB in osmotic stress adaptation. Fungal infection assays on citrus fruit revealed that ∆PdMpkB proliferated poorly within host tissues, induced water-soaking lesions, failed to break through host cuticle layers and thus, failed to produce aerial hyphae and conidia. Introduction of a functional copy of PdMpkB into a null mutant restored all defective phenotypes. Transcriptome analysis revealed that inactivation of PdMpkB impacted expression of the genes associated with cell wall-degrading enzyme activities, carbohydrate and amino acid metabolisms, conidial formation, and numerous metabolic processes. Our results define pivotal roles of the PdMpkB-mediated signaling pathway in developmental and pathological functions in the citrus postharvest pathogen P. digitatum.
URI: http://hdl.handle.net/11455/94131
Appears in Collections:植物病理學系

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