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dc.contributor.authorYang, Siwy Lingzh_TW
dc.contributor.authorYu, Pei-Lingzh_TW
dc.contributor.authorChung, Kuang-Renzh_TW
dc.description.abstractThe ability to detoxify reactive oxygen species (ROS) is critical for pathogenicity in the necrotrophic fungus Alternaria alternata. We report a glutathione peroxidase 3 (AaGPx3) involved in the complex signalling network that is essential for the detoxification of cellular stresses induced by ROS and for A. alternata pathogenesis in citrus. AaGPx3 deletion mutants displayed increased sensitivity to H2 O2 and many ROS-generating compounds. AaGPx3 is required for correct fungal development as the AaGPx3 mutant strains showed a severe reduction in conidiation. AaGPx3 mutants accumulated higher chitin content than the wild-type and were less sensitive to the cell wall-targeting compounds calcofluor white and Congo red, as well as the fungicides fludioxonil and vinclozolin, suggesting a role of the glutathione systems in fungal cell wall construction. Virulence assays revealed that AaGPx3 is required for full virulence. The expression of AaGPx3 was downregulated in fungal strains carrying defective NADPH oxidase (Nox) or the oxidative stress responsive regulators YAP1 and HOG1, all implicated in ROS resistance. These results further support the important role of ROS detoxification during A. alternata pathogenesis in citrus. Overall, our study provides genetic evidence to define the central role of AaGPx3 in the biological and pathological functions of A. alternata.zh_TW
dc.relationEnvironmental microbiology, Volume 18, Issue 3, Page(s) 923-35.zh_TW
dc.subjectCell Wallzh_TW
dc.subjectFungal Proteinszh_TW
dc.subjectFungicides, Industrialzh_TW
dc.subjectGlutathione Peroxidasezh_TW
dc.subjectMicrobial Sensitivity Testszh_TW
dc.subjectMolecular Sequence Datazh_TW
dc.subjectNADPH Oxidasezh_TW
dc.subjectOxidative Stresszh_TW
dc.subjectReactive Oxygen Specieszh_TW
dc.titleThe glutathione peroxidase-mediated reactive oxygen species resistance, fungicide sensitivity and cell wall construction in the citrus fungal pathogen Alternaria alternatazh_TW
dc.typeJournal Articlezh_TW
Appears in Collections:植物病理學系


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