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標題: The Function of the SAS10/C1D Family Protein, Thallo, in Plants
作者: 陳瀠莙
Ying-Jiun Chen
關鍵字: Thallo
核醣體DNA variant
核醣體RNA 加工
rDNA variant
rRNA processing
引用: Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, et al. APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis. Plant J. 2010; 64: 960–976. Chandrasekhara C, Mohannath G, Blevins T, Pontvianne F, Pikaard CS. Chromosome-specific NOR inactivation explains selective rRNA gene silencing and dosage control in Arabidopsis. Genes Dev. 2016; 30: 177–190. Chung MC, Lee YI, Cheng YY, Chou YJ, Lu CF. Chromosomal polymorphism of ribosomal genes in the genus Oryza. Theor Appl Genet. 2008; 116: 745–753. Copenhaver GP, Pikaard CS. RFLP and physical mapping with an rDNA-specific endonuclease reveals that nucleolus organizer regions of Arabidopsis thaliana adjoin the telomeres on chromosomes 2 and 4. Plant J. 1996; 9: 259–272. Costello JL, Stead JA, Feigenbutz M, Jones RM, Mitchell P. The C-terminal Region of the Exosome-associated Protein Rrp47 is Specifically Required for Box C/D Small Nucleolar RNA 3’-Maturation. J Biol Chem. 2011; 286: 4535–4543. Deleris A, Greenberg MVC, Ausin I, Law RWY, Moissiard G, Schubert D, et al. Involvement of a Jumonji-C domain-containing histone demethylase in DRM2-mediated maintenance of DNA methylation. EMBO reports. 2919; 11(12): 950-955. Dragon F, Gallagher JEG, Compagnone-Post PA, Mitchell BM, Porwancher KA, Wehner KA, et al. A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature. 2002; 417: 967-970. Durut N, Abou-Ellail M, Pontvianne F, Das S, Kojima H, Ukai S, et al. A Duplicated NUCLEOLIN Gene with Antagonistic Activity Is Required for Chromatin Organization of Silent 45S rDNA in Arabidopsis. Plant Cell. 2014; 26: 1330–1344. Gallagher JEG, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer AL, et al. RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev. 2004; 18: 2506–2517. Granneman S, Baserga SJ. Crosstalk in gene expression: coupling and co-regulation of rDNA transcription, pre-ribosome assembly and pre-rRNA processing. Curr Opin Cell Biol. 2005; 17: 281–286. Hang R, Liu C, Ahmad A, Zhang Y, Lu F, Cao X. Arabidopsis protein arginine methyltransferase 3 is required for ribosome biogenesis by affecting precursor ribosomal RNA processing. Proc Natl Acad Sci. 2014; 111: 16190–16195. Jackson RA, Wu JS, Chen ES. C1D family proteins in coordinating RNA processing, chromosome condensation and DNA damage response. Cell Div. 2016; 11:2. Kamakaka RT, Rine J. Sir- and Silencer-Independent Disruption of Silencing in Saccharomyces by Sas10p. Genetics. 1998; 149: 903–914. Lahmy S, Guilleminot J, Cheng CM, Bechtold N, Albert S, Pelletier G, et al. DOMINO1, a member of a small plant-specific gene family, encodes a protein essential for nuclear and nucleolar functions. Plant J. 2004; 39: 809–820. Lange H, Sement FM, Gagliardi D. MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana. Plant J. 2011; 68: 51–63. Lawrence RJ, Earley K, Pontes O, Silva M, Chen ZJ, Neves N, et al. A Concerted DNA Methylation/Histone Methylation Switch Regulates rRNA Gene Dosage Control and Nucleolar Dominance. Mol Cell. 2004; 13: 599–609. Layat E, Saez-Vasquez J, Tourmente S. Regulation of Pol I-Transcribed 45S rDNA and Pol III-Transcribed 5S rDNA in Arabidopsis. Plant Cell Physiol. 2012; 53(2): 267–276. Lim YH, Charette JM, Baserga SJ. Assembling a Protein-Protein Interaction Map of the SSU Processome from Existing Datasets. PLoS One. 2011; 6: e17701. McClintock B. The relation of a particular chromosomal element to the development of the nucleoli in Zea mays. Z Zellforsch Mikr Anat. 1934; 21: 283–327. Mitchell P. Rrp47 and the function of the Sas10/C1D domain. Biochem Soc Trans. 2010; 38: 1088–1092. Mitchell P, Petfalski E, Houalla R, Podtelejnikov A, Mann M, Tollervey D. Rrp47p is an Exosome-associated Protein Required for the 3’ Processing of Stable RNAs. Mol Cell Biol. 2003; 23: 6982–6992. Moss, T., Langlois, F., Gagnon-Kugler, T., Stefanovsky, V. (2007). A housekeeper with power of attorney: the rRNA genes in ribosome biogenesis. Cell. Mol. Life Sci. 64, 29–49. Ohbayashi I, Konishi M, Ebine K, Sugiyama M. Genetic identification of Arabidopsis RID2 as an essential factor involved in pre-rRNA processing. Plant J. 2011; 67: 49–60. Padeken J, Mendiburo MJ, Chlamydas S, Schwarz HJ, Kremmer E, Heun P. The Nucleoplasmin Homolog NLP Mediates Centromere Clustering and Anchoring to the Nucleolus. Mol Cell. 2013; 50: 236–249. Park SK, Lim JH, Kang CJ. Crlz1 activates transcription by mobilizing cytoplasmic CBFβ into the nucleus. BBA-Gene Regul Mech. 2009; 1789: 702–708. Peng JC, Karpen GH. H3K9 methylation and RNA interference regulate nucleolar organization and repeated DNA stability. Nat Cell Biol. 2007; 9: 25–35. Pontvianne F, Abou-Ellail M, Douet J, Comella P, Matia I, Chandrasekhara C, et al. Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana. PLoS Genet. 2010; 6: e1001225. Pontvianne F, Blevins T, Chandrasekhara C, Mozgová I, Hassel C, Pontes OMF, et al. Subnuclear partitioning of rRNA genes between the nucleolus and nucleoplasm reflects alternative epiallelic states. Genes Dev. 2013; 27: 1545–1550. Pontvianne F, Matía I, Douet J, Tourmente S, Medina FJ, Echeverria M, et al. Characterization of AtNUC-L1 Reveals a Central Role of Nucleolin in Nucleolus Organization and Silencing of AtNUC-L2 Gene in Arabidopsis. Mol Biol Cell. 2007; 18: 369–379. Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, et al. AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana. Plant J. 2011; 65: 807–819. Siroky J, Zluvova J, Riha K, Shippen DE, Vyskot B. Rearrangements of ribosomal DNA clusters in late generation telomerase-deficient Arabidopsis. Chromosoma. 2003; 112: 116–123. Sikorski PJ, Zuber H, Philippe L, Sement FM, Canday J, Kufel J, et al. Distinct 18S rRNA precursors are targets of the exosome complex, the exoribonuclease RRP6L2 and the terminal nucleotidyltransferase TRL in Arabidopsis thaliana. Plant J. 2015; 83: 991-1004. Tschochner H, Hurt E. Pre-ribosomes on the road from the nucleolus to the cytoplasm. Trends Cell Biol. 2003; 13: 255–263. Weis BL, Palm D, Missbach S, Bohnsack MT, Schleiff E. atBRX1-1 and atBRX1-2 are involved in an alternative rRNA processing pathway in Arabidopsis thaliana. RNA. 2015; 21: 415–425. Weis BL, Kovacevic J, Missbach S, Schleiff E. Plant-Specific Features of Ribosome Biogenesis. Trends Plant Sci. 2015; 20: 729-740. Yavuzer U, Smith G, Bliss T, Werner D, Jackson S. DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D. Genes Dev. 1998; 12: 2188–2199. Yoo SD, Cho YH, Sheen, J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc. 2007; 2: 1565–1572.
摘要: The nucleolus is the site of ribosomal RNA (rRNA) gene transcription, rRNA processing, and assembly of mature rRNAs with ribosomal proteins. The rRNA regulatory network underlying the structure and function of the plant nucleolus is largely unknown. We performed an in silico screen for essential genes of unknown function in Arabidopsis thaliana and identified Thallo (THAL) encoding a SAS10/C1D family protein. THAL disruption caused enlarged nucleoli in arrested embryos, aberrant processing of precursor rRNAs at the 5’ External Transcribed Spacer, and repression of the major rDNA variant (VAR1). THAL overexpression lines showed de-repression of VAR1 and overall reversed effects on rRNA processing sites. Strikingly, THAL overexpression also induced formation of multiple nucleoli per nucleus phenotypic of mutants of heterochromatin factors. THAL physically associated with histone chaperone Nucleolin 1 (NUC1), histone-binding NUC2, and histone demethylase Jumonji 14 (JMJ14) in bimolecular fluorescence complementation assay, suggesting that it participates in chromatin regulation. Furthermore, investigation of truncated THAL proteins revealed that the SAS10 C-terminal domain is likely important for its function in chromatin configuration. THAL also interacted with putative Small Subunit processome components, including previously unreported Arabidopsis homologue of yeast M Phase Phosphoprotein 10 (MPP10). Our results uncover the dual role of THAL in transcription and processing events critical for proper rRNA biogenesis and nucleolar organization during reproduction. This is the first study to define the function of SAS10/C1D family members in plants.
真核細胞之核醣體RNA被轉錄、加工,並與核醣體蛋白組裝在核仁中。核醣體RNA的基因轉錄與加工的調控機制在植物中尚不清楚,尤其是其與核仁結構的關係。我們在一個篩選當中找到了一個必要的基因並命名為Thallo (THAL),而其合成的蛋白質屬於SAS10/C1D family。THAL突變造成增大的核仁於停止在球型的胚胎中、異常的5端External Transcribed Spacer核醣體RNA加工,以及主要核醣體DNA variant (VAR1)表現抑制。THAL大量表現則造成VAR1活化和對於核醣體RNA加工相反的作用。顯目地是,THAL大量表現也形成了多個核仁,猶如異染色質的突變株顯型。THAL與組蛋白伴侶分子Nucleolin 1 (NUC1)、結合組蛋白之NUC2,及組蛋白甲基轉移酶Jumonji 14 (JMJ14)皆有相互作用,暗示著其可能參與染色質之調控。除此之外,THAL截短蛋白研究顯示SAS10 C-terminal domain對於其在染色質結構的功能可能相當重要。THAL也與Small Subunit processome分子作用,包括未曾被發表的酵母菌M Phase Phosphoprotein 10 (MPP10)之阿拉伯芥同源蛋白。我們的研究成果揭發了THAL於核醣體RNA生合成必要的轉錄與加工之雙重功能,並進而影響核仁結構與植物繁殖。此篇發表是第一個定義植物SAS10/C1D family蛋白功能的研究著作。
文章公開時間: 2016-11-30
Appears in Collections:生物科技學研究所



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