Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/94798
標題: The pathological role of advanced glycation end products-downregulated heat shock protein 60 in islet β-cell hypertrophy and dysfunction
作者: Guan, Siao-Syun
Sheu, Meei-Ling
Yang, Rong-Sen
Chan, Ding-Cheng
Wu, Cheng-Tien
Yang, Ting-Hua
Chiang, Chih-Kang
Liu, Shing-Hwa
關鍵字: Pathology Section
advanced glycation end products
diabetes
heat shock protein 60
β-cell hypertrophy
Aged
Animals
Cells, Cultured
Chaperonin 60
Diabetes Mellitus, Experimental
Down-Regulation
Glycation End Products, Advanced
Humans
Hypertrophy
Insulin-Secreting Cells
Male
Mice
Oxidative Stress
Rats
Signal Transduction
摘要: Heat shock protein 60 (HSP60) is a mitochondrial chaperone. Advanced glycation end products (AGEs) have been shown to interfere with the β-cell function. We hypothesized that AGEs induced β-cell hypertrophy and dysfunction through a HSP60 dysregulation pathway during the stage of islet/β-cell hypertrophy of type-2-diabetes. We investigated the role of HSP60 in AGEs-induced β-cell hypertrophy and dysfunction using the models of diabetic mice and cultured β-cells. Hypertrophy, increased levels of p27Kip1, AGEs, and receptor for AGEs (RAGE), and decreased levels of HSP60, insulin, and ATP content were obviously observed in pancreatic islets of 12-week-old db/db diabetic mice. Low-concentration AGEs significantly induced the cell hypertrophy, increased the p27Kip1 expression, and decreased the HSP60 expression, insulin secretion, and ATP content in cultured β-cells, which could be reversed by RAGE neutralizing antibody. HSP60 overexpression significantly reversed AGEs-induced hypertrophy, dysfunction, and ATP reduction in β-cells. Oxidative stress was also involved in the AGEs-decreased HSP60 expression in β-cells. Pancreatic sections from diabetic patient showed islet hypertrophy, increased AGEs level, and decreased HSP60 level as compared with normal subject. These findings highlight a novel mechanism by which a HSP60-correlated signaling pathway contributes to the AGEs-RAGE axis-induced β-cell hypertrophy and dysfunction under diabetic hyperglycemia.
URI: http://hdl.handle.net/11455/94798
Appears in Collections:生物醫學研究所

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