Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/94800
標題: Human Rad23A plays a regulatory role in autophagy
作者: Tan, Xiaotong
Wang, Hsin-Chiao
Liang, Ruei-Yue
Chuang, Show-Mei
關鍵字: Autophagy
Chemoresistance
LC3
hHR23A
A549 Cells
Adenine
Antineoplastic Agents
Apoptosis
Autophagy
Beclin-1
Cell Proliferation
Cisplatin
DNA Repair Enzymes
DNA-Binding Proteins
Drug Resistance, Neoplasm
Flow Cytometry
Humans
Immunoblotting
Microscopy, Confocal
Microtubule-Associated Proteins
Organoplatinum Compounds
Protein Binding
RNA Interference
摘要: Chemotherapeutic agents can upregulate autophagy which contributes to the acquisition of chemoresistance and the recurrence of cancer. The involvement of hHR23A in chemoresistance is unknown. In this study, we provide evidence suggesting that hHR23A may regulate autophagy. Knockdown of hHR23A decreased cell growth and increased the resistance in A549 cells to the DNA-damaging agents, cisplatin and oxaliplatin. Measurement of EGFP-LC3 puncta (a marker of autophagy) revealed that autophagy was increased in hHR23A-depleted cells. This effect was augmented by exposure to cisplatin or oxaliplatin. In contrast, the overexpression of hHR23A reversed the levels of autophagy-related proteins to control levels in hHR23A-knockdown cells. Moreover, we observed direct interactions among hHR23A, Beclin 1, and LC3. Finally, 3-methyladenine (3-MA)-induced inhibition of autophagy was found to reverse the sensitivity of hHR23A-knockdown cells to the tested DNA-damaging agents. These results collectively indicated that hHR23A-depleted cells exhibit enhanced autophagy when treated with DNA-damaging agents, perhaps suggesting a basis for the involvement of hHR23A in the acquired chemoresistance of cancer cells. Our study thus reveals a previously unrecognized autophagic function for hHR23A and suggests that it could be a potential therapeutic target for chemosensitizing resistant cancer cells.
URI: http://hdl.handle.net/11455/94800
Appears in Collections:生物醫學研究所

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