請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/94831
標題: Synergism between two amphenicol of antibiotics, florfenicol and thiamphenicol, against Staphylococcus aureus
作者: Wei, C-F
Chang, S-K
Shien, J-H
Kuo, H-C
Chen, W-Y
Chou, C-C
關鍵字: Antibiotic combination
Florfenicol
Staphylococcus aureus
Synergism
Thiamphenicol
Animals
Anti-Bacterial Agents
Cattle
Chickens
Drug Synergism
Female
Mice
Staphylococcal Infections
Staphylococcus aureus
Swine
Thiamphenicol
摘要: Synergistic effects between the same class of antibiotics are rarely reported. In the current study, two amphenicols, namely florfenicol and thiamphenicol, exhibited both in vitro and in vivo synergism against clinical isolates ofStaphylococcus aureusfrom chickens, cattle and pigs. Checkerboard assays on 21S. aureusisolates showed that in 80 per cent of methicillin-susceptibleS. aureus(MSSA) and 82 per cent of methicillin-resistantS. aureus(MRSA) isolates tested, the minimal inhibitory concentration (MIC) of florfenicol could be reduced by 75 per cent (1/4 MIC) or more (up to 1/16 MIC) when combined with 1/2 MIC of thiamphenicol to exhibit antimicrobial activity comparable to the respective drugs at original strength (1×MIC). A synergistic effect (fractional inhibitory concentration index ≤0.5 or ≥2-log10decrease in colony-forming unit/ml in time-kill study) was evident against 30 per cent of MSSA and 45 per cent of MRSA strains tested. A study in mice revealed that the florfenicol/thiamphenicol combination at reduced dosages provided sufficient protection againstS. aureuschallenge. The possible mechanism warrants further study but likely includes the facilitated uptake of thiamphenicol via florfenicol action, and this facilitation was not limited to amphenicol class. The present study may offer new strategy for combination therapy and provide potential alternatives for effective treatment againstS. aureusinfections.
URI: http://hdl.handle.net/11455/94831
顯示於類別:獸醫學系所

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