Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/95658
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dc.contributor.authorKan, Shu-Chenzh_TW
dc.contributor.authorLee, Chih-Chenzh_TW
dc.contributor.authorHsu, Yu-Chezh_TW
dc.contributor.authorPeng, Yu-Hsiangzh_TW
dc.contributor.authorChen, Cheng-Chunzh_TW
dc.contributor.authorHuang, Jia-Junzh_TW
dc.contributor.authorHuang, Jenn-Wenzh_TW
dc.contributor.authorShieh, Chwen-Jenzh_TW
dc.contributor.authorJuang, Tzong-Yuanzh_TW
dc.contributor.author劉永銓zh_TW
dc.contributor.authorLiu, Yung-Chuanzh_TW
dc.date2017-07-26-
dc.date.accessioned2018-10-12T03:14:44Z-
dc.date.available2018-10-12T03:14:44Z-
dc.identifier.urihttp://hdl.handle.net/11455/95658-
dc.description.abstractIn this study, Mg–Al layered double hydride (LDH) was prepared and the impact of LDH addition on the biomass and surfactin yield for cultures of Bacillus subtilis CWS1 was examined. Various solid additives, including montmorillonite (MMT), activated carbon (AC) and LDH, were tested. Among these, LDH triggered the highest surfactin yield at 3789 mg/L. The optimal conditions for surfactin production were 2 g/L LDH supplied on the beginning of a 5-day cultivation, resulting in a 3.8-fold increase in yield. To reveal the mechanism of surfactin enhancement, observation was conducted via the optical microscope. The resulted images showed that when LDH was added, the cells became segmented, short and narrow compared to cells without any addition. It is apparent that LDH exhibits a slightly toxic effect, limiting cell growth. The surviving cells were forced to secrete more surfactin to protect themselves from the harsh environment. Since the cells could no longer grow, their only function was to secrete surfactin into the broth, leading to an extraordinarily high surfactin yield with a low biomass production. This suggests that an alternative means for efficient surfactin production is through the addition of LDH.zh_TW
dc.language.isoen_USzh_TW
dc.publisherJOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERSzh_TW
dc.relationJournal of the Taiwan Institute of Chemical Engineers, Volume 80, November 2017, Pages 10-15zh_TW
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S1876107017303127zh_TW
dc.subjectBacillus subtilis CWS1zh_TW
dc.subjectSurfactinzh_TW
dc.subjectSolid additiveszh_TW
dc.subjectLDHzh_TW
dc.subjectFermentationzh_TW
dc.titleEnhanced surfactin production via the addition of layered double hydroxideszh_TW
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.jtice.2017.06.017zh_TW
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