請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/95709
標題: 耐莖潰瘍病紅龍果品系篩選及耐病育種
Selection and Breeding of Pitaya (Hylocereus spp.) Genotypes Tolerant to Pitaya Stem Canker (Neoscytalidium dimidiatum)
作者: 洪筱雁
Hsiao-Yen Hong
關鍵字: 紅龍果
莖潰瘍病
耐病品系
耐病育種
抗氧化系統
pitaya
pitaya stem canker
resistant line
resistant breeding
antioxidant system
引用: 毛青樺。2008。蟹爪蘭X 病毒與紅龍果X 病毒之分子特性與偵測。國立臺灣大學植物病理與微生物學研究所碩士論文。臺灣臺北。105 pp。 李家輝。2015。紅龍果肉質莖礦物營養調查、花芽誘導與抗莖潰瘍病品系篩選。國立中興大學園藝學系碩士論文。臺灣臺中。113 pp。 李國鏞、李國權、鄧再生。1981。微生物誘發的植物病害與酵素的關條:酵素和植物抗病能力。農林學報 30:33-56。 周佳頤。2016。肉桂醛處理對‘臺農二號’番木瓜果皮轉色、抗氧化物含量及抗氧化酶活性之影響。國立中興大學園藝學系碩士論文。臺灣臺中。53pp。 倪蕙芳、黃巧雯、許淑麗、賴素玉、楊宏仁。2013。紅龍果莖潰瘍病之病原特性及防治藥劑篩選。台灣農業研究 62(3):225–234。 倪蕙芳、黃巧雯、林筑蘋、林靜宜、安寶貞、楊宏仁、蔡志濃。2015。紅龍果莖潰瘍病病原特性及防治研究。台灣紅龍果生產技術改進研討會專刊 81-91。 徐麗霞、裴軼琨、徐韋法。1999。園藝作物鈣素功能。北方園藝 126:15-17。 葉洹瑜。2012。臺灣紅龍果莖部潰瘍病之研究。國立台灣大學植物病理與微生物學研究所碩士論文。臺灣臺北。84 pp。 廖玉琬、徐善德。1999。植物與無機養分。植物生理學。?英文化。臺灣臺北。pp. 79-97。 劉命如、洪建龍、劉瑞芬。2004。引起紅龍果斑駁病徵之Cactus virus X 的鑑 定與免疫檢測。植病會刊 13: 27-34。 蔡志濃。2013。紅龍果的重要病害及其防治。農業試驗所技術服務 95:1-7。 蔡怡芳。2004。紅龍果莖腐病病因之探討。國立屏東科技大學植物保護系碩 士論文。臺灣屏東。121 pp。 顏昌瑞。1985。紅龍果。pp. 173-176。台灣農家要覽(農作篇二)。財團法人豐年社。台北。 Albersheim, P. T., M. Jones, and P. D. English. 1969. Biochemistry of the cell wall in relation to infective processes. Ann. Rev. Phytopathol. 7(1):171-191. Apel, K. and H. Hirt. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal Transduction. Annu. Rev. Plant Biol. 55:373-399. Ashraf, M. and S. Mehmood. 1990. Effects of waterlogging on growth and some physiological parameters of four Brassica species. Plant Soil 121(2):203-209. Barthana, D. and N. Nasir. 2013. Description of symptoms and diseases severity of yellow disease stem rot of red dragin fruit (Hylocereus polyrhizus, L.) in Padang Paraman, West Sumatera. J. Bio. UA. 2:222-228. Bateman, D. F. 1964. An induced mechanism of tissue resistance to polygalacturonase in Rhizoctonia-infected hypocotyls of bean. Phytopathol. 54(3):438-445. Bateman, D. F. and R. D. Lumsden. 1965. Relation of calcium content an nature of the pectic substances in bean hypocotyls of different ages to susceptibility to an isolate of Rhizoctonia solani. Phytopathol. 55:734-738 . Beauchamp, C. and I. Fridovich. 1971. Superoxide dismutase: improved assay and an assay applicable to acrylamide gels. Anal. Biochem. 44(1):276-287. Benzie, I. F. and J. J. Strain. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant Power”: The FRAP assay. Anal. Biochem. 239(1):70-76. Camm, E. L. and G. H. N. Towers. 1973. Review article: Phenylalanine ammonia Iyase. Phytochemistry. 12:961-973. Carvalho, R. L., M. F. Cabral, T. A. Germanoa, W. M. de Carvalhob, I. M. Brasil, M. I. Gallão, C. F. H. Moura, M. M. A. Lopes, and M. R. A. de Miranda. 2016. Chitosan coating with trans-cinnamaldehyde improves structural integrity and antioxidant metabolism of fresh-cut melon. Postharvest Biol. Technol. 113:29-39. Chuang, M. F., H. F. Ni, H. R. Yang, S. L. Shu, S. Y. Lai, and Y. L. Jiang. 2012. First report of stem canker disease of pitaya (Hylocereus undatus, H. polyrhizus) caused by Neoscytalidium dimidiatum in Taiwan. Plant Dis. 96(6):906. Crous, P. W., B. Slippers, M. J. Wingfield, J. Rheeder, W. F. O. Marasas, A. J. L. Philips, A. Alves, T. Burgess, P. Barber, and J. Z. Groenewald. 2006. Phylogenetic lineages in the Botryosphaeriaceae. Studies in Mycology 55:235-253. Dixon, R. A. 2001. Natural products and plant disease resistance. Nature 411(6839): 843–847. Elshafie, A. E. and T. Ba-Omar. 2002. First report of Albiziz lebbeck dieback caused by Scytalidium dimidiatum in Oman. Mycopathologia 154(1):37–40. Georgieva, D. and M. M. Kruleva. 1993. Cytochemical investigation of long-term stored maize pollen. Euphytica 72(1):87–94. Gill, S. S. and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48(12):909-930. Hadwiger, L.A., S .L. Hess, and S. V. Broembsen. 1970. Stimulation of phenylalanine ammonia-Iyase activity and phytoalexin production. Phytopathology 60(2):332-336. Heath, R. L. and L. Packer. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125(1):189-198. Heller, J. and P. Tudzynsk. 2011. Reactive oxygen species in phytopathogenic fungi: signaling, development, and disease. Annu. Rev. Phytopathol. 49:369-390. Hurng, W. P. and C. H. Kao. 1993. Loss of starch and increase of α-amylase activity in leaves of flooded tobacco plants. Plant Cell Physiol. 34(4):531-534. Jaleel, C. A., K. Riadh, R. Gopi, P. Manivannan, J. Ines, H. J. Al-Juburi, Z. Chang-Xing, S. Hong-Bo, and R. Panneerselvam. 2009. Antioxidant defense responses: physiological plasticity in higher plants under abiotic. Acta. Physiol. Plant 31(3):427-436. Jana, S. and M. A. Choudhuri. 1982. Glycolate metabolism of three submersed aquatic angiosperms during ageing. Aquat. Bot. 12:345-354. Johnson, L. B. and B. A. Cunningham. 1972. Peroxidase activity in healthy and leaf-rust-infected leaves. Phytochemistry 11(2):547-551. Kato, M. and S. Shimizu. 1987. Chlorophyll metabolism in higher plants. Ⅶ. Chlorophyll degradation in senescing tobacco leaves: Phenolic-dependent peroxidative degradation. Can. J. Bot. 65(4):729-735. Keith, R. W., D. L. Tourneau, and D. Mahlum. 1958. Quantitative paperchromtographic determination of phenols. J. Chromatogr. 1:534-536. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randal. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193(1):265-275. Metz, C., A. Avinoam, and Y. Mizrahi. 2000. Viability of pollen of two fruit crop cacti of the genus Hylocereus is affected by temperature and duration of storage. Hort. Sci. 35(1): 22-24. Mitchell, H. J., S. A. Hall, R. Stratford, J. L. Hall, and M.S. Barber. 1999. Differential induction of cinnamyl alcohol dehydrogenase during defensive lignification in wheat (Triticum aestivum L.): characterization of the major inducible form. Planta 208(1):31–37. Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7(9):405-410. Mohd, M. S., B. Salleh, and L. Zakaria. 2013. Identification and molecular characterizations of Neoscytalidium dimidiatum causing stem canker of red-fleshed dragon fruit(Hylocereus polyrhizus) in Malaysia. J. Phytopathol. 161(11-12):841-849. Nerd, A. and Y. Mizrahi. 1997. Reproductive Biology of Cactus Fruit Crops. Hortic. Rev. 18:321-346. Polizzi, G., D. Aiello, A. Vitale, F. Giuffrida, J. Z. Groenewald, and P. W. Crous. 2009. First report of shoot blight, canker, and gummosis caused by Neoscytalidium dimidiatum on citrus in Italy. Plant Dis. 93(11):1215. Poovaiah, B. W. 1988. Molecular and cellular aspects of calcium action in plants. Hort. Sci.e 23(2):267-271. Ray, J. D., T. Burgess, and V. M. Lanoiselet. 2010. First record of Neoscytalidium dimidiatum and N. novaehollandiae on Mangifera indica and N. dimidiatum on Ficus carica in Australia. Australas. Plant Dis. Notes 5(1):48-50. Rosen, H. 1957. A modified ninhydrin colorimetric analysis for amino acids. Arch. Biophys. 67(1):10-15. Scandalios, J. G. 2005. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses. Braz. J. Med. Boil. Res. 38(7):995-1014. Shadlea, G. L., S. Varsha, W. Kenneth, L. Kortha, F. Chena, C. Lambb, and R. A. Dixona. 2003. Phenylpropanoid compounds and disease resistance in transgenic tobacco with altered expression of L-phenylalanine ammonia-lyase. Phytochemistry. 64(1):153-161. Shao, X., H. Wang, F. Xu, and S. Cheng. 2013. Effects and possible mechanisms of tea tree oil vapor treatment on the main disease in postharvest strawberry fruit. Postharvest Biol. Technol. 77:94-101. Talmadge, K. W., K. Keegstra, W. D. Bauer, and P. Albersheim. 1973. The structure of plaht cell walls. Plant Physiol. 51:158-173. Tran, D. H. and C. R. Yen. 2014. Morphological characteristics and pollination requirement in red pitaya (Hylocereus spp.). Int. J. Biol. Biomol. Agric. Food Biotechnol. Eng. 8(3):6-10. Volencia-Botín, A. J., J. S. Sandoval-Islas, E. Cárdenas-Soriano, T. J. Michailides, and G. Rendón-Sánchez. 2003. Botryosphaeria dothidea causing stem spots on Hylocereus undatus in Mexico. New Disease Reports 7:25. Wahid, A., S. Gelani, M. Ashraf, and M. R. Foolad. 2007. Heat tolerance in plants: An overview. Environ. Exp. Bot. 61:199-223. Wang, B., J. Wang, H. Liang, J. Yi, J. Zhang, L. Lin, Y. Wu, X. Feng, J. Cao, and W. Jiang. 2008. Reduced chilling injury in mango fruit by 2, 4- dichlorophenoxyacetic acid and the antioxidant response. Postharv. Biol. Technol. 48(2):172-181. Weiss, J., A. Nerd, and Y. Mizrahi. 1994. Flowering Behavior and Pollination Requirements in Climbing Cacti with Fruit Crop Potential. Hort. Sci. 29(12): 1487-1492. White, P. J. and M. R. Broadley. 2003. Calcium in plants. Ann. Bot. 92(4):487-511. Zeng, K., Y. Deng, J. Ming, and L. Deng. 2010. Induction of disease resistance and ROS metabolism in navel oranges by chitosan. Sci. Hortic. 126(2):223–228. Zhou, Y., J. M. Dahler, S. J. R. Undrhill, and R. B. H. Wills. 2003. Enzymes associated with blackheart development in pineapple fruit. Food Chem. 80(4):565-572.
摘要: 近幾年來紅龍果因栽培容易、病蟲害少、適應性極廣,廣受農民喜愛,栽培面積逐年增加。紅龍果多採粗放栽培,對於病蟲害防治甚少,近年來病害對紅龍果的危害逐漸增加,尤其以紅龍果新興病害紅龍果莖潰瘍病(Pitaya stem canker) 危害甚大,栽培管理不當之果園甚至會遭受廢園之命運,目前推薦防治藥劑極少。本試驗篩選抗病品系,以及利用抗病品系作為母本與商業品種進行雜交,期待能選育抗莖潰瘍病之優良品系,並進一步調查耐莖潰瘍病能力相關之植物防禦因子。 本試驗利用病原菌接種於植體,了解紅龍果品系對於病原菌之抗病性,無刺具有較佳之紅龍果莖潰瘍病耐病力,其次為大紅,而以宜蘭紅肉抗病力最差。耐病品系無刺不同成熟度肉質莖皆會遭受病害感染。以耐病品系無刺為母本與現行栽培種越南白肉種與紅肉品系進行雜交,再以無刺×紅肉品系及無刺×越南白肉種雜交後代各240株進行接種試驗,共獲得無刺×紅肉品系雜交後代26株及無刺×越南白肉種23株抗病單株。調查耐病能力相關之植物防禦因子,耐紅龍果莖潰瘍病品系無刺相較於較不耐病品系大紅及宜蘭紅肉,可溶性蛋白質、總游離胺基酸、總酚類化合物、抗壞血酸、抗氧化能力、過氧化物酶具有較高濃度或較高活性,抗壞血酸過氧化物酶活性及比活性則與耐病力相反,過氧化氫濃度、超氧岐化酶活性及比活性、過氧化氫酶活性及比活性、苯丙胺酸裂解酶活性及比活性與元素濃度則與耐病力無明顯相關性。
In recent years, pitaya is widely loved by farmers because of easy cultivation, less pests and diseases, and widely adaptability, and the production area has incresed gradually. Pitaya is extensively cultivated with little effort given to pests and diseases protection. Therefore, the disease problem of to pitaya such as pitaya stem canker has caused great harm to pitaya. Improper cultivation and management often resulted in many pitaya orchards being abandoned. Currently, only few recommended pesticides to control pitaya stem canker are available. In this study, disease-resistant lines with commercial vale, were screened from hybrid offspring of disease-resistant cultivars and commercial varieties, and plant defense factors associated with resistance to pitaya stem canker were investigated. The pathogen of pitaya stem canker was inoculated in the implant to evaluate the disease resistance. Pitaya ‘Wu Ci’ showed a better pitaya stem canker disease resistance, followed by ‘Ta Hong’, and ‘Yi Lan Hong Rou’ demonstrated the worst resistance. Stems of disease-resistant lines ‘Wu Ci’ at different maturity stages all suffered from disease infection. Disease-resistant line ‘Wu Ci’ was then crossbreeded with commercial cultivated varieties ‘Vietnam Bai Rou’ and ‘Hong Rou strain’. Two hundred and forty hybrid offspring each from ‘Wu Ci’ בHong Rou strain’ and ‘Wu Ci’ בVietnam Bai Rou’ were inoculated with Neoscytalidium dimidiatum. Twenty six and 23 disease-resistant lines of ‘Wu Ci’ בHong Rou strain’ and ‘Wu Ci’ בVietnam Bai Rou’ were obtained, respectively. On the other hand, soluble protein (SP), total free amino acid (FAA), total phenolic compound (TPC), ascorbic acid (ASA), antioxidant capacity (FRAP), peroxidase (POD) and peroxidase specific activity of disease-resistant line ‘Wu Ci’ were higher than those of ‘Ta Hong’, and ‘Yi Lan Hong Rou’. In contrast, ascorbate peroxidase activity and ascorbate peroxidase specific activity were inversely related to disease resistance. Hydrogen peroxide content, superoxide dismutase activity, superoxide dismutase specific activity, catalase activity, catalase specific activity, phenylalanine ammonia lyase activity, phenylalanine ammonia lyase specific activity and nutrient element concentration were not correlated with disease resistance.
URI: http://hdl.handle.net/11455/95709
文章公開時間: 2020-08-25
顯示於類別:園藝學系

文件中的檔案:
檔案 大小格式 
nchu-106-7104032103-1.pdf1.25 MBAdobe PDF 請求副本


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。