請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/95718
標題: 非農藥資材對外銷蝴蝶蘭苗控制水苔介質檢疫害蟲之研究
Non-chemical Pesticide Control Pest in Sphagnum Moss Medium of Phalaenopsis Seedlings for Export
作者: 詹凱竣
Kai-Chun Chan
關鍵字: 蝴蝶蘭苗
Phalaenopsis seedlings
cinnamon oil
sucrose fatty acid ester
sphagnum moss
living pest
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摘要: 本研究利用食品級肉桂油及食品添加物脂肪酸蔗糖酯作為非農藥資材,應用於控制輸澳蝴蝶蘭苗株水苔介質活蟲之可行性,期能建立安全、無汙染且能有效殺蟲之非農藥防治方法,提高檢疫合格率。 蝴蝶蘭苗以0.1%肉桂油浸泡處理5分鐘後可完全殺死活蟲,處理後14天藥害指數為4.00;以0.07%肉桂油浸泡3分鐘後,間隔24、48及72小時再以相同條件浸泡第2次可完全殺死活蟲,處理後14天藥害指數在3.25-3.75間。另外,以0.05-0.20%肉桂油噴施蝴蝶蘭苗葉片5次,每次間隔48小時,對於葉片不產生藥害,但亦無法顯著減少介質活蟲數量。而浸泡濃度高達1.00%的脂肪酸蔗糖酯溶液5分鐘,對於減少介質中活蟲沒有顯著效果,根部亦無藥害發生。 為達到殺蟲及減少藥害之目的,本研究測試燻蒸及複合處理之效果。肉桂油燻蒸為全株處理。蝴蝶蘭苗以濃度80 μL·L-1燻蒸8小時無法減少介質中活蟲數量,但也無藥害發生。提高濃度至1000 μL·L-1並延長燻蒸時間至12小時,亦無法減少介質中活蟲數量,但會導致根部發生藥害,處理後10天藥害指數為3.00。 複合處理部分,蝴蝶蘭苗以0.03%、0.02%、0.025%或0.017%肉桂油及1%脂肪酸蔗糖酯複合處理浸泡3分鐘,均能顯著減少介質活蟲數量,處理後14天藥害指數在1.33-1.50間。0.03%肉桂油、1%脂肪酸蔗糖酯及1%或2%煙燻水之複合處理浸泡3分鐘,亦能顯著減少介質活蟲數量,處理後7天藥害指數分別為2.25和2.75。0.10%肉桂油浸泡2分鐘後再浸泡去離子水3分鐘或以2 L去離子水淋洗,均有殺蟲效果且可降低藥害指數,其中0.10%肉桂油浸泡2分鐘後以2 L去離子水淋洗能有效減少介質活蟲數量,且藥害指數能控制在1以下,為最佳複合處理。 0.1%肉桂油浸泡處理2分鐘會誘導蝴蝶蘭苗根部的乙烯釋放率提高,約為對照組的2倍,若以1-MCP預先燻蒸48小時後再浸泡,雖可使乙烯釋放率降低約一半,但外觀上仍有嚴重藥害,顯示肉桂油處理會使根部受傷且誘導乙烯產生,但根部藥害之產生與乙烯並無直接關係。 綜合上述結果顯示,以0.10%肉桂油浸泡2分鐘後,淋洗去離子水2 L之複合處理可顯著減少介質活蟲數量,其結果與商業慣行方法接近,且藥害指數輕微(小於1),分析礦物元素、葉綠素含量及葉綠素螢光顯示,此複合處理並不會顯著影響植株生理狀況。因此,0.1%肉桂油浸泡2分鐘後以2 L去離子水淋洗處理應具有取代目前商業慣行施以化學農藥方法之潛力,可作為未來外銷蝴蝶蘭苗非農藥防治策略之參考,提升檢疫合格率。
In this study, food-grade cinnamon oil and sucrose esters of food additives are used as non-chemical pestcides to control living pests in the sphagnum moss of Phalaenopsis seedlings. We hope to establish a safe, pollution-free and insecticidal method of non-chemical pestcides to improve the pass rate of quarantine. Phalaenopsis seedlings treated with 0.1% cinnamon oil for 5 minutes could completely kill living pests and the phytotoxicity index is 4.00. After treated with 0.07% cinnamon oil for 3 minutes, the living pests were completely killed by treated with the same condition after 24, 48 and 72 hours and the phytotoxicity index was between 3.25-3.75 at 14 days after second treatment. In addition, the leaves of Phalaenopsis seedlings sprayed with 0.05-2.00% cinnamon oil for 5 times at intervals of 48 hours, which didn’t cause phytotoxicity to leaves, but could not reduce the number of living pests. Phalaenopsis seedlings treated with 1.00% sucrose fatty acid ester for 5 minutes had no significant effect on reduction of the number of living pests in sphagnum moss and would not lead to phytotoxicity to roots simultaneously. In order to achieve insecticidal and reduce phytotoxicity, effects of whole plant fumigation and composite treatments were tested in this study. Phalaenopsis seedlings fumigated with 80 μL·L-1 cinnamon oil for 8 hours could not reduce the number of living pests in sphagnum moss and would not contribute to phytotoxicity. Increasing the concentration to 1000 μL·L-1 and prolonging the fumigation time to 12 hours would not reduce the number of living pests in sphagnum moss, but it would bring about phytotoxicity to roots and the index was 3.00 at 10 days after treatment. In composite treatments, Phalaenopsis seedlings treated with 0.03%, 0.025%, 0.02% and 0.017% cinnamon oil with 1% sucrose fatty acid ester for 3 minutes could reduce the number of living pests in sphagnum moss and the phytotoxicity index was between 1.33-1.50 at 14 days after treatment. Phalaenopsis seedlings treated with 0.03% cinnamon oil, 1% sucrose fatty acid ester and 1% or 2% smoke-water composite treatments for 3 minutes also reduced the number of living pests in sphagnum moss and the phytotoxicity index was 2.25 and 2.75 at 7 days after treatments, respectively. After treated with 0.1% cinnamon oil, Phalaenopsis seedlings soaked in deionized water for 3 minutes or treated with 2 L deionized water, the insecticidal effect would be achieved and could reduce the phytotoxicity. Among them, Phalaenopsis seedlings treated with 0.1% cinnamon oil and then watering with 2 L deionized water could reduce the number of living pests significantly and the phytotoxicity index could be controlled to below 1, which was the best one of composite treatments. The ethylene production rate that was 2 times higher than control would be induce after the roots of Phalaenopsis seedlings treated with 0.1% cinnamon oil for 2 minutes. If fumigated with 1-MCP for 48 hours before treatment, the ethylene production rate would decrease by about half. However, there was still a serious phytotoxicity on the appearance. This result indicated that treated with cinnamon oil would casue root injury and induce ethylene production, while there is no direct connection between them. In summary, the number of living pests in sphagnum moss would be reduce significantly by treated with 0.1% cinnamon oil and 2 L deionized water, which insecticidal effect was similar to commercial treatment and phytotoxicity was slight(below 1) . Analysis of mineral elements, Chlorophyll content and Chlorophyll fluorescence showed that this composite treatment would not interfere the physiological status of Phalaenopsis seedlings. Therefore, this composite treatment should have potential to replace the current method of applying chemical pesticides and it can be used as a reference of non-chemical control strategy for Phalaenopsis seedlings for export to improve the pass rate of quarantine in the future.
URI: http://hdl.handle.net/11455/95718
文章公開時間: 2021-07-31

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