Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/95849
標題: Bovine Lactoferrin Inhibits Dengue Virus Infectivity by Interacting with Heparan Sulfate, Low-Density Lipoprotein Receptor, and DC-SIGN
作者: Chen, Jo-Mei
Fan, Yi-Chin
Lin, Jen-Wei
Chen, Yi-Ying
Hsu, Wei-Li
邱賢松
Chiou, Shyan-Song
關鍵字: DC-SIGN
bovine lactoferrin
dengue virus
heparan sulfate
low-density lipoprotein receptor
Animals
Antiviral Agents
Cattle
Cell Adhesion Molecules
Cell Line
Cercopithecus aethiops
Dengue
Dengue Virus
Female
Heparitin Sulfate
Humans
Inhibitory Concentration 50
Lactoferrin
Lectins, C-Type
Mice
Mice, Inbred BALB C
Pregnancy
Receptors, Cell Surface
Receptors, LDL
Receptors, Virus
THP-1 Cells
Vero Cells
Viral Plaque Assay
Virus Attachment
出版社: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
摘要: Bovine lactoferrin (bLF) presents in milk and has been shown to inhibit several viral infections. Effective drugs are unavailable for the treatment of dengue virus (DENV) infection. In this study, we evaluated the antiviral effect of bLF against DENV infection in vivo and in vitro. Bovine LF significantly inhibited the infection of the four serotypes of DENV in Vero cells. In the time-of-drug addition test, DENV-2 infection was remarkably inhibited when bLF was added during or prior to the occurrence of virus attachment. We also revealed that bovine LF blocks binding between DENV-2 and the cellular membrane by interacting with heparan sulfate (HS), dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN), and low-density lipoprotein receptors (LDLR). In addition, bLF inhibits DENV-2 infection and decreases morbidity in a suckling mouse challenge model. This study supports the finding that bLF may inhibit DENV infection by binding to the potential DENV receptors.
URI: http://hdl.handle.net/11455/95849
Appears in Collections:微生物暨公共衛生學研究所

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