Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/96000
標題: Production of bacterial cellulose by using sorghum distillery residue as major nutrient source and application of a novel bioreactor
以高粱酒糟為基質生產細菌纖維素及改良式靜置培養系統之應用
作者: Ching-Ching Hsiao
蕭景卿
關鍵字: 細菌纖維素
紅茶菇
高粱酒糟萃取液
Komagataeibacter rhaeticus
醋酸
酒精
bacterial cellulose
kombucha
the aqueous extracts of sorghum distillery residue
Komagataeibacter rhaeticus
acetic acid
ethanol
引用: Atalla RH,Vanderhart DL, (1984) Native cellulose: a composite of two distinct crystalline forms. Science 223(4633):283–285 Bae S, Shoda M, (2005) Production of bacterial cellulose by Acetobacter xylinum BPR2001 using molasses medium in a jar fermentor. Appl. Microbiol.Biotechnol. 67, 45–51. Brown RM, Willison J, Richardson CL, (1976) Cellulose biosynthesis in Acetobacter xylinum: visualization of the site of synthesis and direct measurement of the in vivo process. Proc Natl Acad Sci 73(12):4565–4569 Brown RM, Willison J, Richardson CL (1976) Cellulose biosynthesis in Acetobacter xylinum: visualization of the site of synthesis and direct measurement of the in vivo process. Proc Natl Acad Sci 73(12):4565–4569 Carreira P, Mendes JA, Trovatti E, Serafim LS, Freire, CS Silvestre, AJ Neto, CP, (2011) Utilization of residues from agro-forest industries in the production of high value bacterial cellulose. Bioresour. Technol. 102, 7354–7360. Charreau H, Foresti ML, Vazquez A, (2013) Nanocellulose patents trends: a comprehensive review on patents on cellulose nanocrystals, microfibrillated and bacterial cellulose. Recent Pat Nanotechnol 7:56–80 Cheng KC, Catchmark JM, Demirci A, (2011). Effects of CMC addition on bacterial cellulose production in a biofilm reactor and its paper sheets analysis. Biomacromolecules, 12(3), 730-736. Ciechanska D, Struszczyk H, Kazimierczak J, Guzinska K, Pawlak M, Kozlowska E, Matusiak G, Dutkiewicz M (2002) New electro-acoustic transducers based on modified bacterial cellulose. Fibres Text East Eur 10:27–30 Czaja W, Romanovicz D, Malcolm Brown R, (2004) Structural investigations of microbial cellulose produced in stationary and agitated culture. Cellulose 11(3–4):403–411 Dehui Lin , Patricia Lopez Sanchez , Rui Li , Zhixi Li, (2014) Production of bacterial cellulose by Gluconacetobacter hansenii CGMCC 3917 using only waste beer yeast as nutrient source. Bioresource Technology 151,113–119 Fu L, Zhang J, Yang G, (2013) Present status and applications of bacterial cellulose-based materials for skin tissue repair. Carbohydrate Polymers, 92(2),1432-1442 Goelzer F, Faria-Tischer P, Vitorino J, Sierakowski MR, Tischer C, (2009) Production and characterization of nanospheres of bacterial cellulose from Acetobacter xylinum from processed rice bark. Mater. Sci. Eng. C 29, 546–551. Ha JH, Shah N, Ul-Islam M, Khan T, Park JK (2011) Bacterial cellulose production from a single sugar a-linked glucuronic acid-based oligosaccharide. Process Biochem 46(9):1717–1723 Hestrin S, Schramm M, (1954) Synthesis of cellulose by Acetobacter xylinum. 2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochem J 58(2):345 Hornung M, Ludwig M, Schmauder HP, (2006) Optimizing the production of bacterial cellulose in surface culture: a novel aerosol bioreactor working on a fed batch principle (part 3). Engineering in Life Sciences, 7(1), 35-41. Hungund B, Prabhu S, Shetty C, Acharya S, Prabhu V, (2013) Production of bacterial cellulose from Gluconacetobacter persimmonis GH-2 using dual and cheaper carbon sources. J. Microb. Biochem. Technol. 5, 031–033. Iguchi M, Yamanaka S, Budhiono A, (2000) Bacterial cellulose—a masterpiece of nature's arts. J Mater Sci35:261–270 Ishihara M, MatsunagaM, Hayashi N, Tisˇler V, (2002) Utilization of D-xylose as carbon source for production of bacterial cellulose. Enzyme Microb Technol 31(7):986–991 Jonas R, Farah LF, (1998) Production and application of microbial cellulose. Polym Degrad Stab 59(1):101–106 Kawaguchi I, Nakamura K, (2007) Make-up tissue paper for removing cosmetics, comprises glycerin impregnated into a tissue paper which consists of pulp fiber, and bacterial cellulose entangled in the pulp interfiber forming a network structure. JP2009077752-A; JP4314292-B2 Keshk S, Sameshima K, (2005) Evaluation of different carbon sources for bacterial cellulose production. Afr J Biotechnol4:478–482 Klemm D, Heublein B, Fink HP, Bohn A, (2005) Cellulose: fascinating biopolymer and sustainable raw material. Angew. Chem. Int. Ed. 44, 3358–3393. Klemm D, Schumann D, Udhardt U, Marsch S, (2001) Bacterial synthesized cellulosee artificial blood vessels for microsurgery. Progress in Polymer Science,26(9), 1561-1603 Kralisch D, Hessler N, Klemm D, Erdmann R, Schmidt W, (2009) White biotechnology for cellulose manufacturingdThe HoLiR concept. Biotechnology and Bioengineering, 105(4), 740-747 Krystynowicz A, Czaja W, Wiktorowska-Jezierska A, Gonc﹐alves-Mis'kiewicz M, Turkiewicz M, Bielecki S, (2002) Factors affecting the yield and properties of bacterial cellulose.J Ind Microbiol Biot 29:189–195 Lisdiyanti P, Navarro R.R, Uchimura T, Komagata K, (2006) Reclassification of Gluconacetobacter hansenii strains and proposals of Gluconacetobacter saccharivorans sp. nov. and Gluconacetobacter nataicola sp. nov. Int. J. Syst. Evol. Microbiol. 56, 2101–2111 Liu M, Zhang M, Lin S, Liu J, Yang Y, Jin Y, (2012) Optimization of extraction parameters for protein from beer waste brewing yeast treated by pulsed electric fields (PEF). African J. Microbiol. Res. 6, 4739–4746 Masaoka S, Ohe T, Sakota N, (1993) Production of cellulose from glucose by Acetobacter xylinum. J Ferment Bioeng75(1):18–22 Matsuoka M, Tsuchida T, Matsushita K, Adachi O, Yoshinaga F(1996) A synthetic medium for bacterial cellulose production by Acetobacter xylinum subsp. sucrofermentans. Biosci Biotechnol Biochem 60:575– 579 Miao CW, Hamad WY, (2013) Cellulose reinforced polymer composites and nanocomposites: a critical review. Cellulose 20:2221–2262 Mikkelsen D, Flanagan BM, Dykes GA, Gidley MJ (2009) Influence of different carbon sources on bacterial cellulose production by Gluconacetobacter xylinus strain ATCC 53524. J Appl Microbiol 107(2):576–583 Nishi Y, Uryu M, Yamanaka S, Watanabe K, Kitamura N,Iguchi M, Mitsuhashi S, (1990) The structure and mechanical properties of sheets prepared from bacterial cellulose. 2. Improvement of the mechanical properties of sheets and their applicability to diaphragms of electroacoustic transducers. J Mater Sci 25:2997–3001 Nishiyama Y, Langan P, Chanzy H, (2002) Crystal structure and hydrogen-bonding system in cellulose Ib from synchrotron X-ray and neutron fiber diffraction.J Am Chem Soc124(31):9074–9082 Okiyama A, Motoki M, Yamanaka S, (1992). Bacterial cellulose II. Processing of the gelatinous cellulose for food materials. Food Hydrocolloids, 6(5), 479-487. Okiyama A, Motoki M, Yamanaka S, (1993). Bacterial cellulose IV. Application to processed foods. Food Hydrocolloids, 6(6), 503-511 Ougiya H, Watanabe K, Morinaga Y, Yoshinaga F, (1997). Emulsion-stabilizing effect of bacterial cellulose. Biosciences Biotechnology and Biochemistry, 61(9),1541-1545 Pae N, (2009) Rotary discs reactor for enhanced production of microbial cellulose.Universiti Teknologi Malaysia, Faculty of Chemical and Natural Resource Engineering. 15732-15739. Rani MU, Appaiah A, (2011) Optimization of culture conditions for bacterial cellulose production from Gluconacetobacter hansenii UAC09. Ann Microbiol 61(4):781–787 Ross P, Mayer R, Benziman M, (1991) Cellulose biosynthesis and function in bacteria. Microbiol Rev 55(1):35–58 Sani A, Dahman Y, (2009) Improvements in the production of bacterial synthesized biocellulose nanofibres using different culture methods. Journal of Chemical Technology and Biotechnology, 85(2), 151e164. Sani A, Dahman Y, (2009). Improvements in the production of bacterial synthesized biocellulose nanofibres using different culture methods. Journal of Chemical Technology and Biotechnology, 85(2), 151-164 Santos SM, Carbajo JM, Quintana E, Ibarra D, Gomez N, Ladero M, Eugenio ME, Villar JC, (2015) Characterization of purified bacterial cellulose focused on its use on paper restoration. Carbohyd Polym 116:173–181 Sherif MAS Keshk, (2014) Bacterial Cellulose Production and its Industrial Applications. Bioprocessing & Biotechniques,Keshk, J Bioproces Biotechniq 4:2 Shi ZJ, Zhang Y, Phillips GO, Yang G, (2014) Utilization of bacterial cellulose in food. Food Hydrocolloid 35:539–545 Sugiyama J, Harada H, Fujiyoshi Y, Uyeda N, (1985) Lattice images from ultrathin sections of cellulose microfibrils in the cellwall ofValonia macrophysaKuぴtz. Planta 166(2):161–168 Sun D, Zhou L, Wu Q, Yang S, (2007) Preliminary research on structure and properties of nano-cellulose. J Wuhan Univ Technol Mater Sci Ed 22(4):677–680 Vandamme E , De Baets S, Vanbaelen A, Joris K, De Wulf P , (1998) Improved production of bacterial cellulose and its application potential. Polym. Degrad.Stab. 59, 93–99. White D, Brown Jr R, Schuerch C, (1989) Cellulose and wood chemistry and technology. In: Proceedings of the tenth cellulose conference Czaja W, Romanovicz D, Malcolm Brown R (2004) Structural investigations of microbial cellulose produced in stationary and agitated culture. Cellulose 11(3–4):403–411 Xiao L, Mai Y, He F, Yu L, Zhang L, Tang H, (2012). Bio-based green composites with high performance from poly (lactic acid) and surface modified microcrystalline cellulose. Journal of Materials Chemistry, 22(31), Yang Huang, Chunlin Zhu, Jia zhi, YangYing, Nie Chun, tao Chen, Dongping Sun, (2014) Recent advances in bacterial cellulose. Cellulose Issue 1:1–30 Yang YK, Park SH, Hwang JW, Pyun YR, Kim YS (1998) Cellulose production by Acetobacter xylinum BRC5 under agitated condition.J Ferment Bioeng 85(3):312–317 Zaar K, (1979) Visualization of pores (export sites) correlated with cellulose production in the envelope of the gramnegative bacterium Acetobacter xylinum. J Cell Biol 80(3):773–777 Zeng X, Small DP, Wan W, (2011) Statistical optimization of culture conditions for bacterial cellulose production by Acetobacter xylinum BPR 2001 from maple syrup. Carbohydr. Polym. 85, 506–513. Zhang W, Chen SY, Hu WL, Zhou BH, Yang ZH, Yin N, Wang HP, (2011) Facile fabrication of flexible magnetic nanohybrid membrane with amphiphobic surface based on bacterial cellulose. Carbohyd Polym 86:1760–1767 Zhu H, Jia S, Yang H, Tang W, Jia Y, Tan Z, (2010) Characterization of bacteriostatic sausage casing: a composite of bacterial cellulose embedded with 3-polylysine. Food Science and Biotechnology, 19(6), 1479-1484. Zimmermann T, Bordeanu N, Strub E, (2010) Properties of nanofibrillated cellulose from different raw materials and its reinforcement potential. Carbohyd Polym 79:1086–1093
摘要: 細菌纖維素 (bacterial cellulose)為一種由微生物所分泌合成之纖維素,與植物來源之纖維素相比,擁有較高純度、持水力、結晶度及生物相容性等特性,因此可被廣泛地利用在食品、化妝品等工業上。然而細菌纖維素之生產成本高,且產率低,使其應用受到侷限。本研究首先篩選得一株具產高細菌纖維素能力之菌株,並尋找廉價之替代基質來源,其次探討此菌株之最適培養條件,以達到降低生產成本及提高細菌纖維素產量之目的。 首先由水果及紅茶菇樣品中篩選可產細菌纖維素之菌株,以高粱酒蒸餾後剩餘物(高粱酒糟)之萃取液及紅茶茶湯作為基本培養基質,探討額外補充營養源 (碳源、氮源)、醋酸、酒精、酚類物質(咖啡因、兒茶素)對細菌纖維素產量之影響。另一方面,探討利用新型培養槽來提升細菌纖維素產量之可行性。 結果顯示篩選自紅茶菇中之Komagataeibacter rhaeticus NCHU R-1具有較佳產細菌纖維素之能力,以二次蒸餾後酒糟萃取液做為基本培養基質,額外補充5%葡萄糖可擁有較佳之細菌纖維素產量,其乾重為1.29±0.08 g / 100 mL。額外添加醋酸或酒精皆可明顯提升細菌纖維素之產量;而添加咖啡因或兒茶素則對細菌纖維素產量無明顯提升。因此較適培養條件為以二次蒸餾後高梁酒糟萃取液做為基本培養基質,添加5%葡萄糖、0.5%醋酸、0.5%酒精,不調整培養液之初始pH (3.68),於30˚C下培養九天可獲得較佳之細菌纖維素產量,乾重為2.86±0.11g/100 mL。以掃描式電子顯微鏡 (scanning electron microscopy)觀察細菌纖維素之結構與其他文獻相比則無明顯不同。在新型培養槽試驗部分,結果雖顯示未能顯著提升細菌纖維素產量,但已完成改良式靜置培養系統之雛型,若能進一步加以改進,於未來開發運用上,具有相當之發展潛力。
Bacterial cellulose is a biopolymer secreted by bacteria. It exhibits many unusual properties including high purity, high water holding capacity, high crystallinity and biological compatibility so that can be widely used in food, and cosmetics industries. However, the application of bacterial cellulose is limited by its high production costs and low level of productivity. Recently, many agricultural wastes have been investigated to reduce the production cost and improve the production yield. The objective of this research is to find out potential strains for the production of bacterial cellulose. Furthermore, to find a cheap source of alternative substrates and the optimal culture conditions to reduce production costs and improve the yield of bacterial cellulose production. The strains were isolated from fruits and kombucha, and the aqueous extracts of sorghum distillery residue and black tea were used as the basic culture medium. Moreover, the effects of additional nutrient sources (i.e. carbon source and nitrogen source), acetic acid, ethanol and phenolic substances (i.e. caffeine and catechins) on bacterial cellulose production were investigated. In addition, a novel static bioreactor were developed to enhance the production of bacterial cellulose. The results showed that Komagataeibacter rhaeticus NCHU R-1 isolated from kombucha, using the second aqueous extracts of sorghum distillery residue as basic medium, and supplementation of 5% glucose had higher bacterial cellulose production with 1.29 ± 0.08 g / 100 mL on dry basis. In the case of supplementing of acetic acid or ethanol can significantly enhance the production of bacterial cellulose. However, supplementing with the caffeine or catechins showed little effect on bacterial cellulose production Therefore, the optimum culture conditions used in this study was as follow: aqueous extracts of the second sorghum distillery residue as the basic medium, supplementing with 5% glucose, 0.5% acetic acid and 0.5% ethanol, and the initial pH of the culture medium was not adjusted (3.68), it can result in 2.86±0.11 g/100 mL on dry basis when fermentation was performed at 30°C for 9 days. The structure of bacterial cellulose observed by scanning electron microscopy was similar with other literatures. The results showed that the production of bacterial cellulose using the prototype of a novel static bioreactor has not been significantly improved. Further research on improvement of the prototype bioreactor is needed.
URI: http://hdl.handle.net/11455/96000
文章公開時間: 10000-01-01
Appears in Collections:食品暨應用生物科技學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.