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|標題:||Production of bacterial cellulose by using sorghum distillery residue as major nutrient source and application of a novel bioreactor|
the aqueous extracts of sorghum distillery residue
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|摘要:||細菌纖維素 (bacterial cellulose)為一種由微生物所分泌合成之纖維素，與植物來源之纖維素相比，擁有較高純度、持水力、結晶度及生物相容性等特性，因此可被廣泛地利用在食品、化妝品等工業上。然而細菌纖維素之生產成本高，且產率低，使其應用受到侷限。本研究首先篩選得一株具產高細菌纖維素能力之菌株，並尋找廉價之替代基質來源，其次探討此菌株之最適培養條件，以達到降低生產成本及提高細菌纖維素產量之目的。
結果顯示篩選自紅茶菇中之Komagataeibacter rhaeticus NCHU R-1具有較佳產細菌纖維素之能力，以二次蒸餾後酒糟萃取液做為基本培養基質，額外補充5%葡萄糖可擁有較佳之細菌纖維素產量，其乾重為1.29±0.08 g / 100 mL。額外添加醋酸或酒精皆可明顯提升細菌纖維素之產量；而添加咖啡因或兒茶素則對細菌纖維素產量無明顯提升。因此較適培養條件為以二次蒸餾後高梁酒糟萃取液做為基本培養基質，添加5%葡萄糖、0.5%醋酸、0.5%酒精，不調整培養液之初始pH (3.68)，於30˚C下培養九天可獲得較佳之細菌纖維素產量，乾重為2.86±0.11g/100 mL。以掃描式電子顯微鏡 (scanning electron microscopy)觀察細菌纖維素之結構與其他文獻相比則無明顯不同。在新型培養槽試驗部分，結果雖顯示未能顯著提升細菌纖維素產量，但已完成改良式靜置培養系統之雛型，若能進一步加以改進，於未來開發運用上，具有相當之發展潛力。|
Bacterial cellulose is a biopolymer secreted by bacteria. It exhibits many unusual properties including high purity, high water holding capacity, high crystallinity and biological compatibility so that can be widely used in food, and cosmetics industries. However, the application of bacterial cellulose is limited by its high production costs and low level of productivity. Recently, many agricultural wastes have been investigated to reduce the production cost and improve the production yield. The objective of this research is to find out potential strains for the production of bacterial cellulose. Furthermore, to find a cheap source of alternative substrates and the optimal culture conditions to reduce production costs and improve the yield of bacterial cellulose production. The strains were isolated from fruits and kombucha, and the aqueous extracts of sorghum distillery residue and black tea were used as the basic culture medium. Moreover, the effects of additional nutrient sources (i.e. carbon source and nitrogen source), acetic acid, ethanol and phenolic substances (i.e. caffeine and catechins) on bacterial cellulose production were investigated. In addition, a novel static bioreactor were developed to enhance the production of bacterial cellulose. The results showed that Komagataeibacter rhaeticus NCHU R-1 isolated from kombucha, using the second aqueous extracts of sorghum distillery residue as basic medium, and supplementation of 5% glucose had higher bacterial cellulose production with 1.29 ± 0.08 g / 100 mL on dry basis. In the case of supplementing of acetic acid or ethanol can significantly enhance the production of bacterial cellulose. However, supplementing with the caffeine or catechins showed little effect on bacterial cellulose production Therefore, the optimum culture conditions used in this study was as follow: aqueous extracts of the second sorghum distillery residue as the basic medium, supplementing with 5% glucose, 0.5% acetic acid and 0.5% ethanol, and the initial pH of the culture medium was not adjusted (3.68), it can result in 2.86±0.11 g/100 mL on dry basis when fermentation was performed at 30°C for 9 days. The structure of bacterial cellulose observed by scanning electron microscopy was similar with other literatures. The results showed that the production of bacterial cellulose using the prototype of a novel static bioreactor has not been significantly improved. Further research on improvement of the prototype bioreactor is needed.
|Appears in Collections:||食品暨應用生物科技學系|
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