Please use this identifier to cite or link to this item:
標題: Development of rapid assays to detect enterotoxin genes of Staphylococcus aureus in foods
作者: Hsin-Yi Yin
關鍵字: 金黃色葡萄球菌
Staphylococcus aureus
immunomagnetic beads
liposomal nanovesicles
multiplex loop-mediated isothermal amplification
lateral flow assay
引用: Ahn-Yoon S, DeCory TR, and Durst RA. (2004) Ganglioside-liposome immunoassay for the detection of botulinum toxin. Anal Bioanal Chem 378, 68-75. Alber G, Hammer DK, and Fleischer B. (1990) Relationship between enterotoxic- and T lymphocytestimulating activity of staphylococcal enterotoxin B. J Immunol 144, 4501-4506. Ang GY, Yu CY, and Yean CY. (2012) Ambient temperature detection of PCR amplicons with a novel sequence-specific nucleic acid lateral flow biosensor. Biosens Bioelectron 38, 151-156. Asao T, Kumeda Y, Kawai T, Shibata T, Oda H, Haruki K, Nakazawa H, and Kozaki S. (2003) An extensive outbreak of staphylococcal food poisoning due to low-fat milk in Japan: estimation of enterotoxin A in the incriminated milk and powdered skim milk. Epidemiol Infect 130, 33-40. Aycicek H, Cakiroglu S, and Stevenson TH. (2005) Incidence of Staphylococcus aureus in ready-to-eat meals from military cafeterias in Ankara, Turkey. Food Control 16, 531-534. Babu D and Muriana PM. (2011) Immunomagnetic bead-based recovery and real time quantitative PCR (RT iq-PCR) for sensitive quantification of aflatoxin B1. J Microbiol Methods 86,188-94. Baird RM and Lee WH. (1995) Media used in the detection and enumeration of Staphylococcus aureus. Int J Food Microbiol 26, 15-24. Balaban N and Rasooly A. (2000) Staphylococcal enterotoxins. Int J Food Microbiol 61, 1-10. Barber MA. (1914) Milk poisoning due to a type of Staphylococcus albus occurring in the udder of a healthy cow. Philipp J Sci 9, 515-519. Bartlett GR. (1959) Phosphorus assay in column chromatography. J Biol Chem 234, 466-468. Baumgartner A, Niederhauser I, Johler S. (2014) Virulence and resistance gene profiles of staphylococcus aureus strains isolated from ready-to-eat foods. J Food Prot 77, 1232-6. Bayer EA, Ben-Hur H, and Wilchek M. (1990) Isolation and properties of streptavidin. Methods Enzymol 184, 80-9. Becker K, Friedrich AW, Lubritz G, Weilert M, Peters G, and Von Eiff C. (2003) Prevalence of genes encoding pyrogenic toxin superantigens and exfoliative toxins among strains of Staphylococcus aureus isolated from blood and nasal specimens. J Clin Microbiol 41, 1434-1439. Bergdoll MS, Surgalla MJ, and Dack GM (1959b) Staphylococcal enterotoxin. Identification of a specific precipitating antibody with enterotoxin-neutralizing property. J Immunol 83, 334-338. Bergdoll MS. (1983) Enterotoxins. In Sataphylococci and staphylococcal infections ed Easton, C.S.F. and Adlam, C. pp. 559-598. London: Academic Press. Betley MJ, Borst DW, and Regassa LB. (1992) Staphylococcal enterotoxins, toxic shock syndrome toxin and streptococcal pyrogenic exotoxins: a comparative study of their molecular biology. Chem Immunol 55, 1-35. Bhunia AK. (2008) Staphylococcus aureus. In Foodborne microbial pathogens. ed Heldman DR. pp. 125-134. Blake RD and Delcourt SG. (1996) Thermodynamic effects of formamide on DNA stability. Nucleic Acids Res 24, 2095-2103. Borsa BA, Tuna BG, Hernandez FJ, Hernandez LI, Bayramoglu G, MY Arica, and Ozalp VC. (2016) Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugates. Biosens Bioelectron 86, 27-32. Bryan FL, Guzewich JJ, and Todd ECD. (1997) Surveillance of foodborne disease II. Summary and presentation of descriptive data and epidemiologic patterns; their value and limitations. J Food Prot 60, 567-578. Casman EP. (1960) Further serological studies of staphylococcal enterotoxin. J Bacteriol 79, 849-856. CDC (Centers for Disease Control and Prevention) (2013) Surveillance for foodborne disease outbreaks - United States, 2009-2010. MMWR Morb Mortal Wkly Rep 62, 1-60. CDC (Centers for Disease Control and Prevention) (2014a) Surveillance for foodborne disease outbreaks - United States, 2011 Annual Report. Atlanta, Georgia: US Department of Health and Human Services, CDC, 1-14. CDC (Centers for Disease Control and Prevention) (2014b) Surveillance for foodborne disease outbreaks - United States, 2012 Annual Report. Atlanta, Georgia: US Department of Health and Human Services, CDC, 1-14. CDC (Centers for Disease Control and Prevention) (2015) Surveillance for foodborne disease outbreaks - United States, 2013 Annual Report. Atlanta, Georgia: US Department of Health and Human Services, CDC, 1-15. Cha JO, Lee JK, Jung YH, Yoo JI, Park YK, Kim BS, and Lee YS. (2006) Molecular analysis of Staphylococcus aureus isolates associated with staphylococcal food poisoning in South Korea. J Appl Microbiol 101, 864-871. Chanteau S, Dartevells S, Mahamane AE, Djibo S, Boisier P, and Nato F. (2006) New rapid diagnostic test for Neisseria meningitidis serogroups A, W135, C, and Y. PLoS Med 3, 1579-1586. Chen X, Gan M, Xu H, Chen F, Ming X, Xu H, Wei H, Xu F, and Liu C. (2014) Development of a rapid and sensitive quantum dot-based immunochromatographic strip by double labeling PCR products for detection of Staphylococcus aureus in food. Food Control 46, 225-232. Chen Y, Cheng N, Xu Y, Huang K, Luo Y, and Xu W. (2016) Point-of-care and visual detection of P. aeruginosa and its toxin genes by multiple LAMP and lateral flow nucleic acid biosensor. Biosens Bioelectron 15, 317-23. Chiang YC, Liao WW, Fan CM, Pai WY, Chiou CS, and Tsen HY. (2008) PCR detection of staphylococcal enterotoxins (SEs) N, O, P, Q, R, U, and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases in Taiwan. Int J Food Microbiol 121, 66-73. Chu PT, and Wen HW. (2013) Sensitive detection and quantification of gliadin contamination in gluten-free food with immunomagnetic beads based liposomal fluorescence immunoassay. Anal Chim Acta 787, 246-53 Chu PT, Hsieh MF, Yin SY, and Wen HW. (2009) Development of a rapid and sensitive immunomagnetic- bead based assay for detection of Bacillus cereus in milk. Eur Food Res Technol 229, 73-81. Collins WS, Johnson AD, Metzger JF, and Bennett RW. (1973) Rapid solid-Phase radioimmunoassay for staphylococcal enterotoxin A. Appl Microbiol. 25, 774-777. Conlan JV, Khounsy S, Blacksell SD, Morrissy CJ, Wilks CR, and Gleeson LJ. (2009) Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen. Trop Anim Health Prod 41, 913-20. Daniel MC and Astruc D. (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104, 293-346. DeCory TR, Durst RA, Zimmerman SJ, Garringer LA, Paluca G, DeCory HH, and Montagna RA. (2005) Development of an immunomagnetic bead-immunoliposome fluorescence assay for rapid detection of Escherichia coli O157:H7 in aqueous samples and comparison of the assay with a standard microbiological method. Appl Environ Microbiol 71, 1856-64. EC (2003) Opinion on Staphylococcal enterotoxins in milk products, particularly cheeses (adopted on 26-27 March 2003). Available at: Accessed 13 May 2016. EC (2005) Commission regulation No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. Official Journal of the European Union L338, 1-26. Edwards KA and Baeumner AJ. (2006) Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization. Anal Bioanal Chem 386, 1335-43. Edwards KA, and Baeumner AJ. (2006) Liposomes in analyses. Talanta 68, 1421-1431. Edwards KA, and Baeumner AJ. (2006) Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization. Anal Bioanal Chem 386, 1335-43. Edwards KA, and Baeumner AJ. (2006) Optimization of DNA-tagged liposomes for use in microtiter plate analyses. Anal Bioanal Chem 386, 1613-23. EFSA (2012) The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2010. EFSA J 10, 1-442. EFSA (2013) The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2011. EFSA J 11, 1-250. EFSA (2014) The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012. EFSA J 12, 1-320. EFSA (2015) The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013. EFSA J 13, 1-165. Fang Z, Wu W, Lu X, and Zeng L. (2014) Lateral flow biosensor for DNA extraction-free detection of Salmonella based on aptamer mediated strand displacement amplification. Biosens Bioelectron 15, 192-7. Foster TJ, Geoghegan JA, Ganesh VK, and Höök M. (2014) Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus. Nat Rev Microbiol 12, 49-62. GB 4789.10 (2010) National food safety standard Food microbiological examination: Staphylococcus aureus. National Standard of the People's Republic of China. Accessed 27 Feb 2017. Gilligan K, Shipley M, Stiles B, Hadfield T, and Ibrahim MS. (2000) Identification of Staphylococcus aureus enterotoxins A and B genes by PCR-ELISA. Mol Cell Probes 14, 71-78. Gomes LI, Dos Santos Marques LH, Enk MJ, de Oliveira MC, Coelho PM, and Rabello A. (2010) Development and evaluation of a sensitive PCR-ELISA system for detection of schistosoma infection in feces. PLoS Negl Trop Dis 4, e664. Goto M, Hayashidani H, Takatori K, and Hara-Kudo Y. (2007) Rapid detection of enterotoxigenic Staphylococcus aureus harbouring genes for four classical enterotoxins, SEA, SEB, SEC and SED, by loop-mediated isothermal amplification assay. Lett Appl Microbiol 45, 100-107. Harris TO, Grossman D, Kappler JW, Marrack P, Rich RR, and Betley MJ. (1993) Lack of complete correlation between emetic and T-cell-stimulatory activities of staphylococcal enterotoxins. Infect Immunol 61, 3175-3183. Hartman PA. (2001). The evolution of food microbiology. In Food microbiology- Fundamentals and Frontiers. Ed. Doyle MP, Beuchat LR, and Montville TJ. pp3-12. Washington D.C: A S M Press. He J, Huang M, Wang D, Zhang Z, Li G. (2014) Magnetic separation techniques in sample preparation for biological analysis: A review. J Pharm Biomed Anal 101, 84-101. He Y, Zeng K, Zhang S, Gurung AS, Baloda M, Zhang X, and Liu G. (2012) Visual detection of gene mutations based on isothermal strand-displacement polymerase reaction and lateral flow strip. Biosens Bioelectron 15, 310-5. Hennekinne JA, De Buyser ML, and Dragacci S. (2012) Staphylococcus aureus and its food poisoning toxins: characterization and outbreak investigation. FEMS Microbiol Rev 36, 815-836. Hennekinne JA, Ostyn A, Guillier F, Herbin S, Prufer AL, and Dragacci S. (2010) How should staphylococcal food poisoning outbreaks be characterized? Toxins 2, 2106-2116. Hiller Y, Gershoni JM, Bayer EA, and Wilchek M. (1987) Biotin binding to avidin. Oligosaccharide side chain not required for ligand association. Biochem J 248, 167-171. Hoffman M, Tremaine M, Mansfield J, and Betley M. (1996) Biochemical and mutational analysis of the histidine residues of staphylococcal enterotoxin A. Infect Immun 64, 885–890. Holecková B, Holoda E, Fotta M, Kalinácova V, Gondol' J, and Grolmus J. (2002) Occurrence of enterotoxigenic Staphylococcus aureus in food. Ann Agric Environ Med 9, 179-182. Hovde CJ, Marr JC, Hoffmann ML, Hackett SP, Chi YI, Crum KK, Stevens DL, Stauffacher CV, and Bohach GA. (1994) Investigation of the role of the disulphide bond in the activity and structure of staphylococcal enterotoxin C1. Mol Microbiol 13, 897-909. Hu DL, and Nakane A. (2014) Mechanisms of staphylococcal enterotoxin-induced emesis. Eur J Pharmacol 5, 95-107. Huang Y, Chen X, Duan N, Wu S, Wang Z, Wei X, and Wang Y. (2015) Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1. Food Chem 1, 623-9. Huang Y, Zhang H, Chen X, Wang X, Duan N, Wu S, Xu B, and Wang Z. (2015) A multicolor time-resolved fluorescence aptasensor for the simultaneous detection of multiplex Staphylococcus aureus enterotoxins in the milk. Biosens Bioelectron 15, 170-6. Jay JM, Loessner MJ, and Golden DA. (2008) Staphylococcal gastroenteritis. In Modern Food Microbiology. Ed. Jay JM, Loessner MJ, and Golden DA. p551. New York: Springer. Jin W, Yamada K, Ikami M, Kaji N, Tokeshi M, Atsumi Y, Mizutani M, Murai A, Okamoto A, Namikawa T, Baba Y, and Ohta M. (2013) Application of IgY to sandwich enzyme-linked immunosorbent assays, lateral flow devices, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products. J Microbiol Methods 92, 323-31. Jung JH, Oh SJ, Kim YT, Kim SY, Kim WJ, Jung J, and Seo TS. (2014) Combination of multiplex reverse-transcription loop-mediated isothermal amplification with an immunochromatographic strip for subtyping influenza A virus. Anal Chim Acta 853, 541-547. Jung Y, Jeong JY, and Chung BH. (2008) Recent advances in immobilization methods of antibodies on solid supports. Analyst 133, 697-701. Jung YK, Kim TW, Jung C, Cho DY, and Park HG. (2008) A polydiacetylene microchip based on a biotin-streptavidin interaction for the diagnosis of pathogen infections. Small 4, 1778-84. Kérouanton A, Hennekinne JA, Letertre C, Petit L, Chesneau O, Brisabois A, and De Buyser ML. (2007) Characterization of Staphylococcus aureus strains associated with food poisoning outbreaks in France. Int J Food Microbiol 115, 369-75. Khan AS and Cao CJ. Thompson RG, and Valdes JJ. (2003) A simple and rapid fluorescence-based immunoassay for the detection of Staphylococcal enterotoxin B. Mol Cell Probes 17, 125-126. Kim GY and Son A. (2010) Development and characterization of a magnetic bead-quantum dot nanoparticles based assay capable of Escherichia coli O157:H7 quantification. Anal Chim Acta 677, 90-96. Korpela J. (1984) Avidin, a high affinity biotin-binding protein, as a tool and subject of biological research. Med Biol 62, 5-26. Langevin PB, Gravenstein N, Doyle TJ, Roberts SA, Skinner S, Langevin SO, and Gulig PA. Growth of Staphylococcus aureus in diprivan and intralipid: implications on the pathogenesis of infections. Anesthesiology 91, 1394-1400. Lasch L, Weissing V, and Brandl M. (2003) Preparation of liposomes. In Liposomes: a practical approach. Ed Torchilin VP, Weissig V. pp 3-29. New York: Oxford University Press. Leserman LD, Barbet J, Kourilsky F, and Weinstein JN. (1980) Targeting to cells of fluorescent liposomes covalently coupled with monoclonal antibody or protein A. Nature 288, 602-4. Letertre C, Perelle S, Dilasser F, and Fach P. (2003) Detection and genotyping by real-time PCR of the Staphylococcal enterotoxin genes SEA to SEJ. Mol Cell Probes 17, 139-147. Levine WC, Bennett RW, Choi Y, Henning KJ, Rager JR, Hendricks KA, Hopkins DP, Gunn RA, and Griffin PM. (1996) Staphylococcal food poisoning caused by imported canned mushrooms. J Infect Dis 173,1263-1267. Lewis K. (2001) Riddle of biofilm resistance. Antimicrob Agents Chemother 45, 999-1007. Li J and Macdonald J. (2016) Multiplexed lateral flow biosensors: Technological advances for radically improving point-of-care diagnoses. Biosens Bioelectron 15, 177-92. Li L, Han Y, and Liu J. (2013) Assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools. Environ Monit Assess 185, 603-613. Lindroth S and Niskanen A. (1977) Double antibody solid-phase radioimmunoassay for staphylococcal enterotoxin A. Eur J Apple Microbiol 4,137-143. Liu CC, Yeung CY, Chen PH, Yeh MK, and Hou SY. (2013) Salmonella detection using 16S ribosomal DNA/RNA probe-gold nanoparticles and lateral flow immunoassay. Food Chem 1, 2526-32. Liu N, Li X, Ma X, Ou G, and Gao Z. (2014) Rapid and multiple detections of staphylococcal enterotoxins by two-dimensional molecularly imprinted film-coated QCM sensor. Sens Actuators B Chem 191, 326-331. Liu P, Han L, Wang F, Petrenko VA, and Liu A. (2016) Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus. Biosens Bioelectron 15, 195-203. Lü JH, Li HK, An HJ, Wang GH, Wang Y, Li MQ, Zhang Y, and Hu J. (2004) Positioning isolation and biochemical analysis of single DNA molecules based on nanomanipulation and single-molecule PCR. J Am Chem Soc 126, 11136-7. Mehrotra M, Wang G, and Johnson WM. (2000) Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol 38, 1032-1035. Morissette C, Goulet J, and Lamoureux G. (1991) Rapid and sensitive sandwich enzyme-linked immunosorbent assay for detection of Staphylococcal enterotoxin B in cheese. Appl Environ Microbiol 57, 836-842. Munson SH, Tremaine MT, Betley MJ, and Welch RA. (1998) Identification and characterization of staphylococcal enterotoxin types G and I from Staphylococcus aureus. Infect Immun 66, 3337-3348. Nagarajappa S, Thakur MS, and Manonmani HK. (2012) Detection of enterotoxigenic Staphylococci by loop-mediated isothermal amplification method. Journal of Food Safety 32, 59-65. Nato F, Boutonnier A, Rajerison M, Grosjean P, Dartevelle S, et al. (2003) One step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples. Clin Diagn Lab Immunol 10, 476-478. Nato F, Phalipon A, Nguyen TL, Diep TT, Sansonetti P, and Germani Y. (2007) Dipstick for rapid diagnosis of Shigella flexneri in stool. PLoS Med 2, 1-7. New RRC. (1990) Preparation of liposomes. In Liposomes: a practical approach. Ed New RRC. pp 33-102. New York: Oxford University Press. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, and Hase T. (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28, E26. Nurul Najian AB, Engku Nur Syafirah EA, Ismail N, Mohamed M, and Yean CY. (2016) Development of multiplex loop mediated isothermal amplification (m-LAMP) label-based gold nanoparticles lateral flow dipstick biosensor for detection of pathogenic Leptospira. Anal Chim Acta 15, 142-8. Omoe K, Ishikawa M, Shimoda Y, Hu DL, Ueda S, and K Shinagawa. (2002) Detection of seg, seh, and sei genes in Staphylococcus aureusisolates and determination of the enterotoxin productivities of S. aureus isolates harboring seg, seh, or sei genes. J Clin Microbiol 40, 857-62. Palmer ED. (1951) The morphologic consequences of acute exogenous (Staphylococci) gastroenteritis on the gastric mucosa. Gastroenterology 19, 462-475. Park CE and Szabo R. (1986) Evaluation of the reversed passive latex agglutination (RPLA) test kits for detection of Staphylococcal enterotoxins A, B, C, and D in foods. Can J Microbiol 32, 723-727. Park CE, Akhtar M, and Rayman MK. (1992) Nonspecific reactions of a commercial enzyme-linked immunosorbent assay kit (TECRA) for detection of Staphylococcal enterotoxins in food. Appl Environ Microbiol 58, 2509-2512. Paul N, and Yee J. (2010) PCR incorporation of modified dNTPs: the substrate properties of biotinylated dNTPs. Biotechniques 48, 333-334. Presnova GV, Rubtsova MY, Presnov DE, Grigorenko VG, Yaminsky IV, and Egorov AM. (2014) Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface. Biomed Khim 8, 164-167. Read RBJr and Bradshaw JG. (1966a) Thermal inactivation of staphylococcal enterotoxin B in veronal buffer. Appl Microbiol 14, 130-32. Read RBJr and Bradshaw JG. (1966b) Staphylococcal enterotoxin B thermal inactivation in milk. J Dairy Sci 49, 202-203. Reddy P, Ramlal S, Sripathy MH, and Batra HV. (2014) Development and evaluation of IgY ImmunoCapture PCR ELISA for detection of Staphylococcus aureus enterotoxin A devoid of protein A interference. J Immunol Methods 408, 114-22. Reddy PK, Shekar A, Kingston JJ, Sripathy MH, and Batra H. (2013) Evaluation of IgY capture ELISA for sensitive detection of alpha hemolysin of Staphylococcus aureus without staphylococcal protein A interference. J Immunol Methods 391, 31-38. Reiser RF, Robbins RN, Khoe GP, and Bergdoll MS. (1983) Purificaton and some physicochemical properties of toxic-shock toxin. Biochemistry 22, 3907-12. Ren K, Bannan JD, Panchi V, Cheung AL, Robbins JC, Fischetti VA, and Zabrskie JB. (1994) Characterisation and properties of a new staphylococcal exotoxin. J Exp Med 180,1675-1683. Resch A. Rosenstein R, Nerz C, and Gotz F. (2005) Differential gene expression profiling of Staphylococcus aureus cultivated under biofilm and planktonic conditions. Appl Environ Microbiol 71, 2663-2676. Ríos A, and M Zougagh. (2016) Recent advances in magnetic nanomaterials for improving analytical processes. Trends Anal Chem 84, 72-83. Rodríguez A, Gordillo R, Andrade M, Córdoba J, and Rodríguez M. (2016) Development of an efficient real-time PCR assay to quantify enterotoxin-producing Staphylococci in meat products. Food Control 60, 302-308. Rodríguez A, Gordillo R, Andrade MJ, Córdoba JJ, and Rodríguez M. (2016) Development of an efficient real-time PCR assay to quantify enterotoxin-producing staphylococci in meat products. Food control 60, 302-308. Rose SA, Bankes P, and Stringer M. (1989) Detection of Staphylococcal enterotoxins in dairy products by the reversed passive latex agglutination (SET-RPLA) kit. Int J Food Microbiol 8, 65-72. Sharma A, Rao VK, Kamboj DV, Gaur R, Upadhyay S, and Shaik M. (2015) Relative efficiency of zinc sulfide (ZnS) quantum dots (QDs) based electrochemical and fluorescence immunoassay for the detection of Staphylococcal enterotoxin B (SEB). Biotechnol Rep 6, 129-136. Shin J and Kim M. (2008) Development of liposome immunoassay for Salmonella spp. using immunomagnetic separation and immunoliposome. J Microbiol Biotechnol 18, 1689-1694. Shingaki M, Igarashi H, Fujikawa H, Ushioda H, Terayama T, and Sakai S. (1981) Study on reversed passive latex agglutination for the detection of staphylococcal enterotoxins A-C. Annu Rep Tokyo Metr Res Lab Publ Hlth 32, 128-131. Singh PK, Agrawal R, Kamboj DV, Gupta G, Boopathi M, Goel AK, and Singh L. (2010) Construction of a single-chain variable-fragment antibody against the superantigen Staphylococcal enterotoxin B. Appl Environ Microbiol 519, 8184-8191. Stelma GN and Bergdoll MS. (1982) Inactivation of staphylococcal enterotoxin A by chemical modification. Biochem Biophys Res Commun 105, 121–126. Stewart PS and Costerton JW. (2001). Antibiotic resistance of bacteria in biofilms. Lancet 358, 135-138. Tamanaha CR, Mulvaney SP, Rife JC, and Whitman LJ. (2008) Magnetic labeling, detection, and system integration. Biosens Bioelectron 24, 1-13. Tasara T, Angerer B, Damond M, Winter H, Dörhöfer S, Hübscher U, and Amacker M. (2003) Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. II. High-density labeling of natural DNA. Nucleic Acids Res 31, 2636-46. Tempelman LA, King KD, Anderson GP, and Ligler FS. (1996) Quantitating Staphylococcal enterotoxin B in diverse media using a portable fiber-optic biosensor. Anal Biochem 233, 50-57. Tsen HY, Yu GK, Wang KC, Wang SJ, Chang MY, and Lin LY. (1998) Comparison of the enterotoxigenic types, toxic shock syndrome toxin I (TSST-1) strains and antibiotic susceptibilities for enterotoxigenic Staphylococcus aureusstrains isolated from food and clinical samples. Food Microbiol 15, 33-41. Vaidya HC, Wolf BA, Garrett N, Catalona WJ, Clayman RV, and Nahm MH. (1988) Extremely high values of prostate-specific antigen in patients with adenocarcinoma of the prostate; demonstration of the 'hook effect'. Clin Chem 34, 2175-2177. Van Roy N, Mangelschots K, and Speleman F. (1993) Improved immunocytochemical detection of biotinylated probes with Neutralite avidin. Trends Genet 9, 71-2. Wang W, Liu L, Xu L, Kuang H, Zhu J, and Xu C. (2016) Gold-nanoparticle-based multiplexed immunochromatographic strip for simultaneous detection of staphylococcal enterotoxin A, B, C, D, and E. Part Part Syst Charact 33, 388-395. Watanabe K, Kuwata N, Sakamoto H, Amano Y, Satomura T, and Suye S. (2015) A smart DNA sensing system for detecting methicillin-resistant Staphylococcus aureus using modified nanoparticle probes. Biosens Bioelectron 15, 419-23. Wellman AD, and Sepaniak MJ. (2006) Magnetically-assisted transport evanescent field fluoroimmunoassay. Anal Chem 78, 4450-4456. Wen HW, Decory TR, Borejsza-Wysocki W, and Durst RA, (2006) Investigation of NeutrAvidin-tagged liposomal nanovesicles as universal detection reagents for bioanalytical assays. Talanta 68, 1264-1272. Wieneke AA, Roberts D, and Gilbert RJ. (1993) Staphylococcal food poisoning in the United Kingdom, 1969-90. Epidemiol Infec 110, 519-531. Wu L, Gao B, Zhang F, Sun X, Zhang Y, and Li Z. (2013) A novel electrochemical immunosensor based on magnetosomes for detection of staphylococcal enterotoxin B in milk. Talanta 15, 360-6. Xu M, Wang R, and Li Y. (2016) Rapid detection of Escherichia coli O157:H7 and Salmonella Typhimurium in foods using an electrochemical immunosensor based on screen-printed interdigitated microelectrode and immunomagnetic separation. Talanta 148, 200-8. Yang S, Ouyang H, Su X, Gao H, Kong W, Wang M, Shu Q, and Fu Z. (2016) Dual-recognition detection of Staphylococcus aureus using vancomycin-functionalized magnetic beads as concentration carriers. Biosens Bioelectron 15, 174-80. Yin HY, Chu PT, Tsai WC, and Wen HW. (2016) Development of a barcode-style lateral flow immunoassay for the rapid semi-quantification of gliadin in foods. Food Chem 192, 934-942. Yin HY, Fang TJ, Wen HW. (2016) Combined multiplex loop-mediated isothermal amplification with lateral flow assay to detect sea and seb genes of enterotoxic Staphylococcus aureus. Lett Appl Microbiol 63(1), 16-24. Yin R, Sun Y , Yu S , Wang Y , Zhang M , Xu Y, Xue J, and Xu N. (2016) A validated strip-based lateral flow assay for the confirmation of sheep-specific PCR products for the authentication of meat. Food Control 60, 146-150. Yu J, Zhang Y, Zhang Y, Li H, Yang H, and Wei H. (2016) Sensitive and rapid detection of Staphylococcus aureus in milk via cell binding domain of lysine. Biosens Bioelectron 15, 366-71. Yue H, Zhou Y, Wang P, Wang X, Wang Z, Wang L, and Fu Z. (2016) A facile label-free electrochemiluminescent biosensor for specific detection of Staphylococcus aureus utilizing the binding between immunoglobulin G and protein A. Talanta 153, 401-406. Zacco E, Pividori MI, Alegret S, Galve R, and Marco MP. (2006) Electrochemical magnetoimmunosensing strategy for the detection of pesticides residues. Anal Chem 78, 1780-8. Zaytseva NV, Montagna RA, and Baeumner AJ. (2005) Microfluidic biosensor for the serotype-specific detection of dengue virus RNA. Anal Chem 77, 7520-7. Zhang C, Liu Z, Li Y, Li Q, Song C, Xu Z, Zhang Y, Zhang Y, Ma Y, Sun Y, Chen L, Fang L, Yang A, Yang K, and Jin B. (2013) High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin A in multi-matrices. Anal Chim Acta 24, 14-9. Zhang X, Lowe SB, and Gooding JJ. (2014) Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP). Biosens Bioelectron 15, 491-9. Zhong Z, Peng N, Qing Y, Shan J, Li M, Guan W, Dai N, Gu X, and Wang D. (2011) An electrochemical immunosensor for simultaneous multiplexed detection of neuron-specific enolase and pro-gastrin-releasing peptide using liposomes as enhancer. Electrochimica Acta 55, 5624-5629. Zouharova M and Rysanek D. (2008) Multiplex PCR and RPLA identification of Staphylococcus aureus enterotoxigenic strains from bulk tank milk. Zoonoses Public Health 55, 313-9.
摘要: In Taiwan, among various staphylococcus enterotoxins (SEs) produced by Staphylococcus aureus, SEA is the most related to food poisoning outbreaks, followed by SEB. In the United States, annually there are 185,000 cases of foodborne illnesses caused by SEs. Until now, more than 20 kinds of SEs have been reported. SEs have the superantigenic activities and can resist to heat and proteases in human gastrointestinal tract. The syndromes caused by SEs include diarrhea, vomiting, retching, abdominal cramping and prostration, and a toxin dose of less than 1 μg will cause these symptoms. Therefore, the purpose of this study is to develop rapid assays for detecting enterotoxic S. aureus in food. First, we developed an immunomagnetic bead (IMB) based method with the use of NeutrAvidin-tagged liposomal nanovesicles (NA-LNs) which encapsulated fluorescent dyes as the detection reagent to detect S. aureus containing sea gene. Through a PCR reaction, the target DNA was amplified and labeled with digoxigenin (Dig) and biotin. The amplified target DNA was then captured by IMB modified with anti-Dig-antibody and detected by NA-LNs. The developed assay could detect S. aureus and differentiate it from Salmonella enterica and Escherichia coli, with a limit of detection (LOD) of 101 CFU mL-1 without pre-enrichment. With a 2-hour pre-enrichment, this developed assay could detect as little as 1 CFU in 25 mL of milk. Furthermore, we developed a multiplex loop-mediated isothermal amplification (m-LAMP) combined with a lateral flow assay (LFA) for simultaneously detecting the sea and seb genes of enterotoxic S. aureus. The LOD of this assay was 102 CFU mL-1 S. aureus, which was 10-fold lower than that of a multiplex PCR; and this assay did not show any cross-reactivity as detecting other enterotoxic S. aureus strains or other food pathogens. After 4~6-hour enrichment, this developed assay could detect as low as 1 CFU mL-1 of S. aureus in four different food matrixes - milk, apple juice, cheese, and rice. Conclusively, these two developed methods can be completely finished within a workday, which can provide an alternative way to easily and quickly screen the contamination of enterotoxic S. aureus in food products or in food supply chains.
文章公開時間: 10000-01-01
Appears in Collections:食品暨應用生物科技學系



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.