Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/96036
標題: Anti-enterovirus 71 activity and relevant mechanism of glycyrol in Rhabdomyosarcoma cells
甘草醇對腸病毒71型於人類橫紋肌肉瘤細胞之抑制作用及相關作用機轉
作者: Huai-En Huang
黃懷恩
關鍵字: 甘草醇
腸病毒71型
作用機轉
病毒複製
glycyrol
enterovirus 71
relevant mechanism
viral replication
引用: 楊心宜。(2014)。七葉一枝花抗腸病毒71型活性成分之分離純化及作 用機轉 (碩士論文)。國立中興大學。 Adianti M, Aoki C, Komoto M, Deng L, Shoii I, Wahyuni TS, Lusida MI, Soetjipto, Fuchino H, Kawahara N, and Hotta H. Anti-hepatitis C virus compounds obtained from Glycyrrhiza uralensis and other Glycyrrhiza species. Microbiol. Immunol. (2014), 58: 180-187. Ahmad N and Mukhtar H. Cytochrome p450: a target for drug development for skin diseases. J. Invest. Dermatol. (2004), 123: 417-425. Akira S and Takeda K. Toll-like receptor signaling. Nat. Rev.Immunol. (2004), 4: 499-511. Appleton I, Tomlinson A, and Willoughby DA. Induction of cyclo- oxygenase and nitric oxide synthase in inflammation. Adv. Pharmacol. (1996), 35: 27–78. Arita M, Takebe Y, Wakita T, and Shimizu H. A bifunctional anti-enterovirus compound that inhibits replication and the early stage of enterovirus 71 infection. J. Gen. Virol. (2010), 91: 2734-2744. Arita M, Wakita T, and Shimizu H. Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infectivity. J. Gen. Virol. (2008), 89: 2518-2530. Autret A, Martin-Latil S, Brisac C, Mousson L, Colbere-Garapin F, and Blondel B. Early phosphatidylinositol 3-kinase/Akt pathway activation limitspoliovirus-induced JNK-mediated cell death. J. Virol. (2008), 82: 3796–3802. Barnard DL. Current status of anti-picornavirus therapies. Curr. Pharm. Des. (2006), 12: 1379-1390. Basavappa R, Syed R, Flore O, Icenogle JP, Filman DJ, and Hogle JM. Role and mechanism of the maturation cleavage of VP0 in poliovirus assembly: structure of the empty capsid assembly intermediate at 2.9 A resolution. Protein. Sci. (1994), 3: 1651–1669. Blondel B, Couderc T, and Delpeyroux F. Enterovirus. J. Neurol. Sci. (2014), 65-69. Brown BA and Pallansch MA. Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus. Virus. Res. (1995), 39:195-205. Chang LY. Enterovirus 71 in Taiwan. Pediatr. Neonatol. (2008), 49: 103-112. Chang LY, Huang LM, Gas SS, Wu YY, Hsia SH, Fan TY, Lin KL, Huang YC, Lu CY and Lin TY. Neurodevelopment and cognition in children after enterovirus 71 infection. N. Engl. J. Med. (2007), 356: 1226-1234. Chang SC, Lin JY, Lo LY, Li ML, and Shih SR. Diverse apoptotic pathways in enterovirus 71-infected cells. J. Neurovirol. (2004), 10: 338-349. Chen B, Sumi A, Toyoda S, Hu Q, Zhou D, Mise K, Zhao J, and Kobayashi N. Time series analysis of reported cases of hand, foot, and mouth disease from 2010 to 2013 in Wuhan, China. BMC. Infect. Dis. (2015), 15: 495. Chen HF, Chang MH, Chiang BL, and Jeng ST. Oral immunization of mice using transgenic tomato fruit expressing VP1 protein from enterovirus 71. Vaccine. (2006), 24: 2944-2951.  Chen TC, Lai YK, Yu CK, and Junag JL. Enterovirus 71 triggering of neuronal apoptosis through activation of Abl-Cdk5 signalling. Cell. Microbiol. (2007), 9: 2676-2688. Chen TC, Liu SC, Huang PN, Chang HY, Chern JH, and Shih SR. Antiviral activity of pyridyl imidazolidinones against enterovirus 71 variants. J. Biomed. Sci. (2008), 15: 291-300. Chen TC, Weng KF, Chang SC, Lin JY, Huang PN, and Shih SR. Development of antiviral agents for enterovirus. J. Antimicrob. Chemother. (2008), 62: 1169-1173. Chen YJ, Zeng SJ, Hsu JT, Horng JT, Yang HM, Shih SR, Chu YT, and Wu TY. Amantadine as a regulator of internal ribosome entry site. Acta. Pharmacol. Sin. (2008), 29: 1327-1333. Chen Z, Li R, Xie Z, Huang G, Yuan Q, and Zneg J. IL-6, IL-10, IL-13 are associated with pathogenesis in children with Enterovirus 71 infection. Int. J. Clin. Exp. Med. (2014), 7: 2718-2723. Chiang LC, Ng LT, Cheng PW, Chiang W, and Lin CC. Antiviral activities of extracts and selected pure constituents of Ocimum basilicum. Clin. Exp. Pharmacol. Physiol. (2005), 32: 811-816. Chiu CH, Chu C, He CC, and Lin TY. Protection of neonatal mice from lethal enterovirus 71 infection by maternal immunization with attenuated Salmonella enterica serovar Typhimurium expressing VP1 of enterovirus 71. Microbes. Infect. (2006), 8: 1671-1678.  Chou AH, Liu CC, Chang JY, Jiang R, Hsieh YC, Tsao A, Wu CL, Huang JL, Fung CP, Hsieh SM, Wang YF, Wang JR, Hu MH, Chiang JR, Su IJ, and Chong PC. Formalin-inactivated EV71 vaccine candidate induced cross-neutralizing antibody against subgenotypes B1, B4, B5 and C4A in adult volunteers. PLoS. One. (2013), 8(11): e79783. Cho HK, Lee NY, Lee H, Kim HS, Seo JW, Hong YM, Lee SJ, Lee SW, Cheon DS, Hong JY, Kang BH, Kim JH, and Kim KH. Enterovirus 71-associated hand, foot and mouth diseases with neurologic symptoms, a university hospital experience in Korea, (2009) Korean. J. Pediatr. (2010), 53: 639-643. Cho SY, Lee SH, Choi JY, Myoung SE, Kang SS, Jeong JS, and Jeong CS. Antigastritic effectof carbenoxolone dismdium from Glycyrrhizae Radix. Toxicol. Res. (2007), 23: 165-172. Choi HJ, Lim CH, Song JH, Baek SH, and Kwon DH. Antiviral activity of raoulic acid from Raoulia australis against Picornaviruses. Phytomedicine. (2009), 16: 35-39. Choi HJ, Song JH, Park KS, and Baek SH. In vitro anti-enterovirus 71 activity of gallic acid from Woodfordia fruticose flower. Lee. Appl. Microbiol. (2010), 50: 438-440. Chong P, Liu CC, Chow YH, Chou AH, and Klein M. Review of enterovirus 71 vaccines. Clin. Infect. Dis. (2015), 60: 797-803. Chua KB and Kasri AR. Hand foot and mouth disease due to enterovirus 71 in Malaysia. Virol. Sin. (2011), 26: 221-228. Chung P, Huang Y, Chang L, Lin T and Ning H. Duration of enterovirus shedding in stool. J. Microbiol. Immunol. Infect. (2001), 34: 167-170. Curry S, Fry E, Blakemore W, Abu-Ghazaleh R, Jackson T, King A, Lea S, Newman J and Stuart D. Dissecting the roles of VP0 cleavage and RNA packaging in picornavirus capsid stabilization: the structure of empty capsids of foot-and-mouth disease. J. Virol. (1997), 71: 9743-9752.  Desdouets C, Matesic G, Molina CA, Foulkes NS, Sassone-Corsi P, Brechot C, and Sobczak-Thepot J. Cell cycle regulation of cyclin A gene expression by the cyclic AMP-responsive transcription factors CREB and CREM. Mol. Cell. Biol. (1995), 15: 3301–3309. Diana GD, Otto MJ, and McKinlay MA. Inhibitors of picornavirus uncoating as antiviral agents. Pharmacol. Ther. (1985), 29: 287-297. Donato C, Hoi le t, Hoa NT, Hoa TM, Van Duyet L, Dieu Ngan TT, Van Kinh N, Vu Trung N, and Vijaykrishna D. Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012. Virology. (2016), 495: 1-9. Duff MF. Hand-foot-and-mouth syndrome in humans: coxackie A10 infections in New Zealand. Br. Med. J. (1968), 2: 661-664. Eerdunbayaer, Orabi MA, Aoyama H, Kuroda T, and Hatano T. Structures of new phenolics isolated form licorice, and the effectiveness of licorice phenolics on vancomycin-resistant Enterococci. Molecules. (2014), 19: 13027-13041. Everett H and McFadden G. Apoptosis: and innate immune response to virus infection. Trends. Microbiol. (1999), 7: 160-165. Fang W, Lin X, Zhou X, Wan J, Lu X, Yang B, Ai W, Lim J, Zhang T, Tu Z, and Liu Y. Cytotoxic and antiviral nitrobenzoyl sesquiterpenoids from the marine-derived fungus Aspergillus ochraceus JcmaF17. Med. Chem. Commun. (2014), 5: 701-705. Falah N, Monserret R, Lelogeais V, Schuffenecker I, Lina B, Cortay JC, and Violot S. Blocking human enterovirus71 replication by targeting viral 2A protease. J. Antimicrob. Chemother. (2012), 67: 2865-2869. Fu Y, Zhou H, Wang S, and Wei Q. Glycyrol suppresses collagen-induced arthritis by regulateing autoimmune and inflammatory responses. PLoS. One. (2014), 9: e98137. Han J, Wang Y, Gan X, Song J, Sun P, and Dong XP. Serum cytokine profiles of children with human enterovirus 71-associated hand, foot, and mouth disease. J. Med. Virol. (2014), 86: 1377-1385. Ho HY, Cheng ML, Weng SF, Leu YL, and Chiu DT. Antiviral effect of epigallocatechin gallate on enterovirus 71. J. Agric. Food. Chem. (2009), 57: 6140-6147. Ho M, Chen ER, Hsu KH, Twu SJ, Chen KT, Tsai SF, Tsai SF, Wang JR, and Shih SR. An epidemic of enterovirus 71 infection in Taiwan. N. Engl. J. Med. (1999), 341: 929-935. Huang CC, Liu CC, Chang YC, Chen CY, Wang ST, and Yeh TF. Neurologic complication in children with enterovirus 71 infection. N. Engl. J. Med. (1999), 341: 936-942. Huang SC, Chang CL, Wang PS, Tsai Y, and Liu HS. Enterovirus 71-induced autophagy detected in virto and in vivo promotes viral replication. J. Med. Virol. (2009), 81: 1241-1252. Huang SW, Hsu YW, Smith DJ, Kiang D, Tsai HP, Lin KH, Wang SM, Liu CC, Su IJ, and Wang JR. Reemergence of enterovirus 71 in 2008 in taiwan: dynamics of genetic and antigenic evolution from 1998 to 2008. J. Clin. Microbiol. (2009), 47: 3653-3662.  Hung HC, Chen TC, Fang MY, Yen KJ, Shih SR, Hsu JT, and Tseng CP. Inhibition of enterovirus 71 replication and viral 3D polymerase by aurinticarboxylic acid. J. Antimicrob. Chemother. (2010), 65: 676-683. Hsueh C, Jung SM, Shih SR, Kuo TT, Shieh WJ, Zaki S, Lin TY, Chang LY, Ning HC, and Yen DC. Acute and fatal encephalomyelitis during an outbreak of enterovirus type 71 infection in Taiwan: report of an autopsy case with pathologic, immunofluorescence and molecular studies. Mod. Pathol. (2000), 13: 1200-1205. Johnson GL and Lapadat R. Mitogen-Actívated Protein Kinase Pathways Mediated by ERK, JNK, and p38 Protein Kinases. Science. (2002), 298: 1911-1912. Ishitani T, Takaesu G, Ninomiya-Tsuji J, Shibuya H, Gaynor RB, and Matsumoto K. Role of the TAB2-related protein TAB3 in IL-1 and TNF signaling. EMBO. J. (2003), 22: 6277-6288. Jin G, Klika A, Callahan M, Faga B, Danzig J, Jiang Z, Li X, Stark GR, Harrington J, and Sherf B. Role of the TAB2-related protein TAB3 in IL-1 and TNF signaling. Proc. Natl. Acad. Sci. USA. (2004), 101: 2028-2033. Kim AH, Khursigara G, Sun X, Franke TF, and Chao MV. Akt phosphorylatesand negatively regulates apoptosis signal-regulating kinase 1. Mol. Cell. Biol. (2001), 21: 893–901. Kim SJ, Kim JH, Kang JH, Kim DS, Ki HK, Kim KH, Kim YH, Chung Bin JH, Jung DE, Kim HJ, Kim MH, Cheon DS, Kang BH, and Seo SY. Risk Factors for Neurologic Complications of Hand, Foot and Mouth Disease in the Republic of Korea, 2009. J. Korean. Med. Sci. (2013), 28: 120-127. Klein T, Shephard P, Kleinert H, and Komhoff M. Regulation of cyclooxygenase-2 expression by cyclic AMP. Biochim. Biophys. Acta. (2007), 1773: 1605-1618. Krampe B and Al-Rubeai M. Cell death in mammalian cell culture: molecular mechanisms and cell line engineering strategies. Cytotechnology. (2010), 62: 175-188. Kusano A, Nikaido T, Kuge T, Ohmoto T, Delle Monache G, Botta B, Botta M, and Saitoh T. Inhibition of adenosine 3', 5'-cyclic monophosphate phosphodiesterase by flavonoids from licorice roots and 4-arylcoumarins. Chem. Pharm. Bull. (Tokyo). (1991), 39: 930-933. Lee S, Oh HM, Lim WB, Choi EJ, Park YN, Kim JA, Choi JY, Hong SJ, Oh HK, Son JK, Lee SH, Kim OJ, Choi HR, and Jun CD. Gene induction by glycyrol to apoptosis through endonuclease G in tumor cells and prediction of oncogene function by microarray analysis. Anticancer. Drugs. (2008) 19: 503-515. Lei K, Nimnual A, Zong WX, Kennedy NJ, Flavell RA, Thompson CB, Bar-Sagi D, and Davis RJ. The Bax subfamily of Bcl2-related proteins is essential for apoptotic signal transduction by c-Jun NH2-Terminal kinase. Mol. Cell. Biol. (2002), 22: 4929-4942. Li HI, Zhu H, Xu CJ, and Yuan JY. Cleavage of BID by caspase 8 mediates the mitochonfrial damage in the Fas pathway of apoptosis. Cell. (1998), 94: 491-501. Li J, Tu Y, Tong L, Zhang W, Zheng J, and Wei Q. Immunosupressive activity on the murine immune responses of glycyrol from Glycyrrhiza uralensis via inhibition of calcineurin activity. Pharm. Biol. (2010), 48: 1177-1184. Li T and Peng T. Traditional Chinese herbal medicine as a source of molecules with antiviral activity. Antiviral. Res. (2013), 97: 1-9. Li ZH, Li CM, Ling P, Shen FH, Chen SH, Liu CC, Yu CK, and Chen SH. Ribavirin reduces mortality in enteovirus 71-infected mice by decreasing viral replication. J. Infect. Dis. (2008), 197: 854-857. Liang CC, Sun MJ, Lei HY, Chen SH, Yu CK, Liu CC, Wang JR, and Yeh TM. Human endothelial cell activation and apoptosis induced by enterovirus 71 infection. J. Med. Virol. (2004), 74: 597-603. Lin TY, Chang LY, Huang YC, Hsu KH, Chiu CH, and Yang KD. Different proinflammatory reactions in fatal and nonfatal enterovirus 71 infections: implications for early recognition and therapy. Acta. Paediatr. (2002), 91: 632-635. Lin TY, Chu C, and Chiu CH. Lactoferrin inhibits enterovirus 71 infection of human embryonal rhabdomyosarcoma cells in vitro. J. Infect. Dis. (2002), 186: 1161-1164. Lin TY, Hsia SH, Huang YC, Wu CT, and Chang LY. Proinflammatory cytokine reactions in enterovirus 71 infections of the central nervous system. Clin. Infect. Dis. (2003), 36: 269−74. Lin TY, Liu YC, Jheng JR, Tsai HP, Jan JT, Wong WR, and Horng JT. Anti-enterovirus 71 activity screening of Chinese herbs with anti-infection and inflammation activities. Am. J. Chin. Med. (2009), 37: 143-158. Lin YC, Juan HC, and Cheng YC. Ozone exposure in the culture medium inhibits enterovirus 71 virus replication and modulates cytokine production in rhabdomyosarcoma cells. Antiviral. Res. (2007), 76: 241-251. Lin YJ, Lai CC, Lai CH, Sue SC, Lin CW, Hung CH, Lin TH, Hsu WY, Huang SM, Hung YL, Tien N, Liu X, Chen CL, and Tsai FJ. Inhibition of enterovirus 71 infection s and viral IRES activity by Fructus gardenia and geniposide. Eur. J. Med. Chem. (2013), 62: 206-213. Lin YW, Wang SW, Tung YY and Chen SH. Enterovirus 71 infection of human dendritic cells. Exp. Biol. Med. (Maywood) (2009), 234: 1166-1173. Liu L, Zhang Y, Wang J, Zhao H, Jiang L, Che Y, Shi H, Li R, Mo Z, Huang T, Liang Z, Mao Q, and Wang L. Study of the integrated immune response induced by an inactivated EV71 vaccine. PLOS. One. (2013), 8: e54451. Liu M, Tao L, Chau SL, Wu R, Zhang H, Yang Y, Yang D, Bian Z, Lu A, Han Q, and Xu H. Folding fan mode counter-current chromatography offers fast screening for drug discovery. Case study: finding anti-enterovirus 71 agents form Anermarrhena asphodeloides. J. Chromatogr. A. (2014), 1368: 116124. Lu WW, Hsu YY, Yang JY, and Kung SH. Selective inhibition of enterovirus 71 replication by short hairpin RNAs. Biochem. Biophys. Res. Commun. (2004), 325: 494-499. MA, Martinez-Martinez S, Punzon C, Redondo JM, and Fresno M. J. Biol. Chem. (2000), 275 : 23627–23635. Ma E, Fung C, Yip SH, Wong C, Chuang SK, and Tsang T. Estimation of the basic reproduction number of enterovirus 71 and coxsackievirus A16 in hand, foot, and mouth disease outbreaks. Pediatr. Infect. Dis. J. (2011), 30: 675–679. Maier JA, Hla T, and Maciag T. Cyclooxygenase is an immediate-early gene induced by interleukin-1 in human endothelial cells. J. Biol. Chem. (1990), 265: 10805-10808. McMinn PC. An overview of the evolution of enterovirus 71 and its clinical and public health significance. FEMS. Microbiol. Rev. (2002), 26: 91-107. Meng T and Kwang J. Attenuation of human enterovirus 71 high-replication-fidelity variants in AG129 mice. J. Virol. (2014), 88: 5803-5813. MiZuta K, Aoki Y, Matoba Y, Yahagi K, Itagaki T, Katsushima F, Katsushima Y, Ito S, Hongo S, and Matsuzaki Y. Molecular epidemiology of enterovirus 71 strains isolated from children in Yamagata, Japan, between 1990 and 2013. J. Med. Microbiol. (2014), 63: 1356- 1362.  Nishimura Y, Lee H, Hafenstein S, Kataoka C, Wakita T, Bergelson JM, and Shimizu H. Enterovirus 71 binding to PSGL-1 on leukocytes: VP1-145 acts as a molecular switch to control receptor interaction. PLoS. Pathog. (2013), 9: e1003511. Nishimura Y, Shimojima M, Tano Y, Miyamura T, Wakita T, and Shimizu Human P-selectin glycoprotein ligand-1 is a functional receptor for enterovirus 71. Nat. Med. (2009),15: 794-797. Nomura Taro, Fukai Toshio, and Hano Yoshio. Chemistry and Biological Activities of Isoprenylated Flavonoids from Medicinal Plants Moraceous Plants and Glycyrrhiza Species. J. Nat. Prod. (2003), 28: 199-256. Ong KC, Devi S, Cardosa MJ, and Wong KT. Formaldehyde-inactivated whole-virus vaccine protects a murine model of enterovirus 71 encephalomyelitis against disease. J. Virol. (2010), 84: 661-665.  Ong KC and Wong KT. Understanding Enterovirus 71 Neuropathogenesis and its impact on Other Neurotropic Enterovirus. Brain. Pathol. (2015), 25: 614-624. Ooi MH, Wong SC, Lewthwaite P, Cardosa MJ, and Solomon T. Clinical features, diagnosis, and management of enterovirus 71. Lancet. Neurol. (2010), 9: 1097–1105. Pallansch MA and Ross RP. Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In: Knipe DM, Howley PM, Griffi n DE, et al, eds. Fields. Virology, vol 1. Hagerstown, MD, USA: Lippincott, Williams & Wilkins, (2001), 1: 723–75. Park SA, Kim EH, Na HK, and Surh YJ. KG-135 Inhibits COX-2 Expression by Blocking the Activation of JNK and AP-1 in Phorbol Ester-Stimulated Human Breast Epithelial Cells. Ann. N. Y. Acad. Sci. (2007), 1095: 545-553. Patel KP and Bergelson JM. Receptors identified for hand, foot and mouth virus. Nat. Med. (2009), 15: 728-729. Peng H, Shi M, Zhang L, Li Y, Sun J, Zhang L, Wang X, Xu X, Zhang X, Mao Y, Ji Y, Jiang J, and Shi W. Activation of JNK1/2 and p38 MAPK signaling pathways promotes enterovirus 71 infection in immature dendritic cells. BMC. Microbiol. (2014), 14: 147. Peng L, Qi Y, Wu H, and Wei Q. Interaction of glycyrol with calcineurin A studied by spectroscopic method and docking. IUBMB. Life. (2011), 63: 14-20. Qiao X, Ji S, Yu SW, Lin XH, Jin HW, Duan YK, Zhang LR, Guo DA, and Ye M. Identification of key licorice constituents which interact with cytochrome P450: evaluation by LC/MS/MS cocktail assay and metabolic profiling. AAPS. J. (2014), 16: 101-113. Ren XX, Ma L, Liu QW, Li C, Huang Z, Wu L, Xiong SD, Wang JH, and Wang HB. The molecule of DC-SIGN captures enterovirus 71 and confers dendritic cell-mediated viral trans-infection. Virol. J. (2014), 11: 47. Rokhlin OW, Glover RA, and Cohen MB. Fas-mediated apoptosis in human prostatic carcinoma cell lines occurs via activation of caspase-8 and caspase-7. Cancer. Res. (1998), 58: 5870-5875. Roulston A, Marcellus RC, and Branton PE. Virus and apoptosis. Annu. Rev.(1999), 53: 577-628. Rossmann MG. Viral cell recognition and entry. Prot. Sci. (1994), 3: 1712-1725. Rossmann MG, Arnold E, Erickson JW, Frankenberger EA, Griffith JP, Hecht HJ, Johnson JE, Kamer G, Luo M, and Mosser AG. Structure of a human common cold virus and functional relationship to other picornaviruses. Nature. (1985), 317: 145-153. Rossmann MG, He Y, and Kuhn RJ. Picornavirus-receptor interactions. Trends. Microbiol. (2002), 10: 324-331. Ryu YB, Kim JH, Park SJ, Chang JS, Rho MC, Bae KH, Park KH, and Lee WS. Inhibition of neuraminidase activity by polyphenol compounds isolated from the roots of Glycyrrhiza uralensis. Bioorg. Med. Chem. Lett. (2010), 1: 971-974. Shi W, Li X, Hou X, Peng H, Jiang Q, Shi M, Ji Y, Liu X, and Liu J. Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection. BMC. Infect. Dis. (2012), 12: 327. Shia KS, Li WT, Chang CM, Hsu MC, Chern JH, Leong MK, Tseng SN, Lee CC, Lee YC, Chen SJ, Peng KC, Tseng HY, Chang YL, Tai CL, and Shih SR. Design, synthesis, and structure-activity relationship of pyridyl imidazolidinones: a novel class of potent and selective human enterovirus 71 inhibitors. J. Med. Chem. (2002), 45; 1644-1655. Shih RS, Chen SJ, Hakimelahi GH, Liu HJ, Tseng CT and Shia KS. Selective human enterovirus and rhinovirus inhibitors: An overview of capsid-binding and protease-inhibiting moleclues. Med. Res. Rev. (2004), 24: 449-474. Shih SR, Tsai MC, Tseng SN, Won KF, Shia KS, Li WT, Chern JH, Chen GW, Lee CC, Lee YC, Peng KC, and Chao YS. Mutation in enterovirus 71 capsid protein VP1 confers resistance to the inhibitory effects of pyridyl imidazolidinone. Antimicrob. Agents. Chemother. (2004), 48: 3523-3529. Shin EM, Kim S, MerfortI, and Lim YS. Glycyrol induces apoptosis in human Jurkat T cell lymphocytes via the Fas-FasL/caspase-8 pathway. Planta. Med. (2011), 77: 242-247. Shin EM, Zhou HY, Guo LY, Kim JA, Lee SH, Merfort I, Kang SS, Kim HS, Kim S, and Kim YS. Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages. Int. Immunopharmacol. (2008), 8: 1524-1532. Shore GC, Papa FR and Oakes SA. Signaling cell death from the endoplasmic reticulum stress response. Curr. Opin. Cell. Biol. 23: 143-149. Sim AC, Luhur A, Tan TM, Chow VT, and Poh CL. RNA interference against enterovirus 71 infection. Virology. (2005), 341: 72-79. Simmons DL, Levy DB, Yannoni Y, and Erikson RL. Identification of a phorbol ester-repressible v-src-inducible gene. Proc. Natl. Acad. Sci. USA. (1989), 86: 1178-1182. Smith WL, Meade EA, and DeWitt DL. Interactions of PGH synthase isozymes-1 and -2 with NSAIDs. Ann. NY. Acad. Sci. (1994), 744: 50-57. Smyth MS and Martin JH. Picornavirus uncoating. Mol. Pathol. (2002): 55, 214-219. Solomon T, Lewthwaite P, Perera D, Cardosa MJ, McMinn P, Ooi MH. Virology, epidemiology, pathogenesis, and control of enterovirus 71. Lancet. Infect. Dis. (2010), 10: 778-790. Song J, Yeo SG, Hong EH, Lee BR, Kim JW, Kim J, Jeong H, Kwon Y, Kim H, Lee S, Park JH, and Ko HJ. Antiviral activity of Hederasaponin B from Hedera helix against Enterovirus 71 subgenotypes C3 and C4a. Biomol. Ther. (Seoul). (2014), 22: 41-46. Tan CW, Poh CL, Sam IC, and Chan YF. Enterovirus 71 uses cell surface heparan sulfate glycosaminoglycan as an attachment receptor. J. Virol. (2013), 87: 611-620. Tan EL, Tan TM, Tak Kwong Chow V, and Poh CL. Inhibition of enterovirus 71 in virus-infected mice by RNA interference. Mol. Ther. (2007), 15: 1931-1938. Tanaka Y, Kikuzaki H, Fukuda S, and Nakatani N. Antibacterial compounds of licorice against upper airway respiratory tract pathogens. J. Nutr. Sci. Vitaminol. (Tokyo). (2001), 47: 270-273. Tournier C, Hess P, Yang DD, Xu J, Turner TK, Nimnual A, Bar-Sagi D, Jones SN, Flavell RA, and Davis RJ. Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway. Science. (2000), 288: 870-874. Tung WH, Hsieh HL, Lee IT and Yang CM. Enterovirus 71 modulates a COX2/PGE2/cAMP-dependent viral replication in human neuroblastoma cells: role of the c-Src/EGFR/p42/p44 MAPK/CREB signaling pathway. J. Cell. Biochem. (2011), 112: 559-570. Tung WH, Lee IT, Hsieh HL, and Yang CM. EV71 induces COX-2 expression via c-Src/PDGFR/PI3K/Akt/p42/p44 MAPK/AP-1 and NF-kappaB in rat brain astrocytes. J. Cell. Physilo. (2010), 224: 376-386. Tung WS, Bakar SA, Sekawi Z, and Rosli R. DNA vaccine constructs against enterovirus 71 elicit immune response in mice. Genet. Vaccines. Ther. (2007), 5:6.  Tsai FJ, Lin CW, Lai CC, Lan YC, Lai CH, Hung CH, Hsueh KC, Lin TH, Chang HC, Wan L, Sheu JJ, and YJ. Kaempferol inhibits enterovirus 71 replication and internal ribosome entry site (IRES) activity through FUBP and HNRP proteins. Food. Chem. (2011), 128: 312-322. Wang H, Zhang D, Ge M, Li Z, Jiang J, and Li Y. Formononetin inhibits enterovirus 71 replication by regulating COX-2/PGE2. Virol. J. (2015), 12:35. Wang J, Chen X, Wang W, Zhang Y, Yang Z, Jin Y, Ge HM, Li E, and Yang G. Glycyrrhizic acid as the antivirial component of Glycyrrhiza uralensis Fisch. Against coxsackievirus A17 and enterovirus 71 of food and month disease. J. Ethnopharmacol. (2003), 147: 113-121. Wang J, Zhang T, Du J, Cui S, Yang F, and Jin Q. Anti-enterovirus 71 effects of chrysin and its phosphate ester. PLoS. One. (2014), 9: e89668. Wang M, Dong Q, Wnag H, He Y, Chen Y, Zhang H, Wu R, Chen X, Zhou B, He J, Kung HF, Huang C, Wei Y, Huang JD, XU H, and He ML. Oblongifolin M, an active cimpound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction throung downregulation of ERp57. Onecotarget. (2016), 7: 8797-8808. Wang SM, Ho TS, Lin HC, Lei HY, Wang JR and Liu CC. Reemerging of enterovirus 71 in Taiwan: the age impact on disease severity. Eur J Clin Microbiol. Infect. Dis. (2012), 31:1219-1224. Wang SM, Lei HY, Huang KJ, Wu JM, Wang JR, Yu CK, Su IJ, and Liu CC. Pathogenesis of enterovirus 71 brainstem encephalitis in pediatric patients: roles of cytokines and cellular immune activation in patients with pulmonary edema. J. Infect. Dis. (2003), 118(4): 564-570. Wang SM and Liu CC. Update of enterovirus 71 infection: epidemiology, pathogenesis and vaccine. Expert. Rev. Anti. Infect. Ther. (2014), 12: 447-456. Wu CN, Lin YC, Fann C, Liao NS, Shih SR, and Ho MS. Protection against lethal enterovirus 71 infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virus. Vaccine. (2001), 20: 895-904.  Wu KX, Ng MM, and Chu JJ. Developments towards antiviral therapies against enterovirus 71. Drug. Discov. Today. (2010), 15: 1041-1051. Wu Y, Yeo A, Phoon MC, Tan EL, Poh CL, Quak SH, and Chow VT. The largest outbreak of hand; foot and mouth disease in Singapore in 2008: the role of enterovirus 71 and coxsackievirus A strains. Int. J. Infect. Dis. (2010), 14: 1076-1081. Wu Z, Yang F, Zhao R, Zhao L, Guo D, and Jin Q. Identification of small interfering RNAs which inhibit the replication of several Enterovirus 71 strains in China. J. Virol. Methods. (2009), 159: 233-238. Xie S, Wang K, Yu W, Lu W, Xu K, Wang J, Ye B, Schwarz W, Jin Q, and Sun B. DIDS blocks a chloride-dependent current that is mediated by the 2B protein of enterovirus 71. Cell. Res. (2011), 21: 1271-1275. Xie WL, Chipman JG, Robertson DL, Erikson RL and Simmons DL. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc. Natl. Acad. Sci. USA. (1991), 88: 2692–2696. Xu L, Su W, Jin J, Chen J, Li X, Zhang X, Sun M, Sun S, Fan P, An D, Zhang H, Zhang X, Kong W, Ma T, and Jiang C. Identification of luteolin as enterovirus 71 and coxsackievirus A16 inhibitors through reporter viruses and cell viability-based screening. Viruses. (2014), 6: 2778-2795. Xu MY and Kim YS. Antitumor activity of glycyrol via induction of cell cycle arrest, apoptosis and defective autophagy. Food. Chem. Toxicol. (2014), 74: 311-319. Xu YR, Ni JM, Meng QG, Zhang CZ, Gao Y, and Wang R.α-glucosidase inhibitors from glycyrrhiza uralensis Fisch. J. Chin. Pharm. Sci. (2006), 15: 24-27. Yamayoshi S, Fujii K, and Koike S. Receptors for enterovirus 71. Emerg. Microbes. Infect. (2014), 3: e53. Yamayoshi S, Yamashita Y, Li J, Hanagata N, Minowa T, Takemura T, and Koike S. Scavenger receptor B2 is a cellular receptor for enterovirus 71. Nat. Med. (2009), 15: 398-401. Yang B, Chuang H, and Yang KD. Sialylated glycans as receptor and inhibitor of enterovirus 71 infection to DLD-1 intestinal cells. Virol. J. (2009), 6: 141. Yang H and Chen C. Cyclooxygenase-2 in synaptic signaling. Curr. Pharm. Des. (2008), 14 : 1443-1451. Yang SL, Chou YT, Wu CN, and Ho MS. Annexin II binds to capsid Protein VP1 of enterovirus 71 and enhances viral infectivity. J. Virol. (2011), 85: 11809-11820. Yang Y, Ma J, Xiu J, Bai L, Guan F, Zhang L, Liu J, and Zhang L. Deferoxamine compensates for decreases in B cell counts and reduces mortality in enterovirus 71-infected mice. Mar. Drugs. (2014), 12: 4086-4095. Yang Y, Xiu J, Liu J, Zhang L, Li X, Xu Y, Qin C, and Zhang L. Chebulagic acid, a hydrolysable tanninm exhibited antiviral activity in vitro and in vivo against human enterovirus 71. Int. J. Mol. Sci. (2013), 14: 9618-9627. Yang Y, Xiu J, Zhang L, Qin C, and Liu J. Antiviral acitivty of punicalagin toward human enterovirus 71 in vitro and in vivo. Phytomedicine. (2012), 20: 67-70. Yang Y, Xiu J, Zhang X, Zhang L, Yan K, Qin C, and Liu J. Antiviral effect of matrine against human enterovirus 71. Molecules. (2012), 17: 10370-10376. Yang Y, Zhang L, Fan X, Qin C, and Liu J. Antiviral effect of geraniin on human enterovirus 71 in virto and in vivo. Bioorg. Med. Chem. Lett. (2012), 22: 2209-2211. Yeo SG, Sing JH, Hong EH, Lee BR, Kwon YS, Chang SY, Kim SH, Lee SW, Park JH, and Ko HJ. Antiviral effects of Phyllanthus urinaria containing corilagin against human enterovirus 71 and Coxsackirvirus A16 in vitro. Arch. Phram. Res. (2015), 38: 193-202. Yi L, Lu J, Kung HF, and He ML. The virology and developments toward control of human enterovirus 71. Crit. Rev. Microbiol. (2011), 73: 313-327. Yip CC, Lau SK, Woo PC, and Yuen KY. Human enterovirus 71 epidemics: what's next? Emerg. Health. Threats. J. (2013), 6: 19780. Yu H, Chen W, Chang H, Tang R, Zhao J, Gan L, Liu B, Chen J, Wnag M. Genetic analysis of the VP1 region of enterovirus 71 reveals the emergence of genotype A in central China in 2008. Virus. Genes. (2010), 41: 1-4. Zeng D, Ma Y, Zhang R, Nei Q, Cui Z, Wang Y, Shang L, and Yin Z. Synthesis and structure-activity relationship of α-keto amides as enterovirus 71 3C protease inhibitors. Bioorg. Med. Chem. Lett. (2016), 26: 1762-1766. Zhang H, Li F, Pan Z, Wu Z, Wang Y, and Cui Y. Activation of PI3K/Akt pathway limits JNK-mediated apoptosis during EV71 infection. Virus. Res. (2014), 192: 74-84. Zhang H, Tao L, Fu WW, Liang S, Yang YF, Yuan QH, Yang DJ, Lu AP, and Xu HX. Prenylated benzoy;phloroglucinols and xanthones from the leaves of Garcinia oblongifolia with antienteroviral activity. J. Net. Prod. (2014), 77: 1037-1046. Zhang L, Li Y, Gu Z,Wang Y, Shi M, Ji Y, Sun J, Xu X, Zhang L, Jiang J, and Shi W. Resveratrol Inhibits Enterovirus 71 Replication and Pro-Inflammatory Cytokine Secretion in Rhabdosarcoma Cells through Blocking IKKs/NF-κB Signaling Pathway. PLoS. One. (2015), 10: e0116879. Zhang W and Liu HT. MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell. Res. (2002), 12: 9-18. Zhang W, Tao J, Yang X, Yang Z, Zhang L, Liu H, Wu K, and Wu J. Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection. Biochem. Biophys. Res. Commun. (2014), 449: 307-312. Zhang W, Qiao H, Lv Y, Wang J, Chen X, Hou Y, Tan R, and Li E. Apigenin inhibits enterovirus-71 infection by disrupting viral RNA association with trans-acting factors. PLoS. One. (2014), 9 : e110429. Zhang X, Song Z, Qin B, Zhang X, Chen L, Hu Y, and Yuan Z. Rupintrivir is a promising candidate for treating severe cases of enterovirus-71 infection: evaluation of antiviral efficacy in a murine infection model. Anticiral. Res. (2013), 97: 264-269. Zhang Y, Liu H, Wang L, Yang F, Hu Y, Ren X, Li G, Yang Y, Sun S, Li Y, Chen X, Li X, and Jin Q. Comparative study of the cytokine/chemokine response in children with differing disease severity in enterovirus 71-induced hand, foot, and mouth disease. PLoS. One. 2013, 8: e67430. Zhao CH, Xu J, Zhang YQ, Zhao LX, and Feng B. Inhibition of human enterovirus 71 replication by pentacyclic triterpenes and their novel synthetic derivatives. Chem. Pharm. Bull. (Tokyo). (2014), 62: 764-771. Zhu F, Xu W, Xia J, Liang Z, Liu Y, Zhang X, Yan X, Wang L, Mao Q, Wu J, Hu Y, Ji T, Song L, Liang Q, Zhang B, Gao Q, Li J, Wang S, Hu Y, Gu S, Zhang J, Yao G, Gu J, Wang X, Zhou Y, Chen C, Zhang M, Cao M, Wang J, Wang H, and Wang N. Efficacy, safety, and immunogenicity of an enterovirus 71 vaccine in China. N. Engl. J. Med. (2014), 370: 818-828.  Zhu FC, Meng FY, Li JX, Li XL, Mao QY, Tao H, Zang YT, Yao X, Chu K, Chen QH, Hu YM, Wu X, Liu P, Zhu LY, Gao F, Jin H, Chen YJ, Dong YY, Liang YC, Shi NM, Ge HM, Liu L, Chen SG, Ai X, Zhang ZY, Ji YG, Luo FJ, Chen XQ, Zhang Y, Zhu LW, Liang ZL, and Shen XL. Efficacy, safety, and immunology of an inactivated alum-adjuvant enterovirus 71 vaccine in children in China: a multicenter, randomized, double-blind, placebo-controlled, phase 3 trial. Lancet. (2013), 381: 2024-2032. Zhu QC, Wang Y, Liu YP, Zhang RQ, Li X, Su WH, Long F, Luo XD, and Peng T. Inhibtion of enterovirus 71 replcation by chrysosplenetin and penduletin. Eur. J. Pharm. Sci. (2011), 44: 392-398.
摘要: 腸病毒71型 (enterovirus, EV71) 為微小核糖核酸病毒科(Picornaviridae) 之腸道病毒。好發於亞洲地區,大多感染學齡前孩童,EV71感染導致手口足病,主要造成中樞神經系統的傷害,嚴重時導致心肺衰竭甚至造成死亡。本實驗室先前由七葉一枝花 (Paris polyphylla Smith) 中分離出高度抑制EV71之活性成分甘草醇 (glycyrol)。本研究接續先前的研究,探討甘草醇抑制EV71之活性及相關作用機轉。 結果顯示,甘草醇對EV71之50%抑制濃度 (50% inhibitory concentration, IC50) 為 1.2 ± 0.01 µg/mL,選擇指數 (selectivity index, SI) 為3.3。在時間點效應中,甘草醇對EV71之抑制作用包含預防病毒感染、直接殺死病毒 (或抑制病毒吸附於宿主細胞) 以及抑制病毒早期之複製。甘草醇具直接致死病毒之活性,2.4 µg/mL處理劑量能使EV71效價有效降低0.33 log PFU/mL (p < 0.05),直接殺死病毒為主要之抑制作用。甘草醇有效抑制子代病毒之複製,於0.6 µg/mL 處理劑量顯著降低0.04 log PFU/mL之子代病毒效價,於2.4 µg/mL 處理劑量能夠有效抑制EV71之VP1和3C蛋白之表現,為EV71感染控制組之34% 及31% (p < 0.01)。甘草醇於2.4 µg/mL 處理劑量對mitogen-activated protein protein kinase (MAPK) (p-p38、p-ERK1/2及p-JNK1/2/3) 具有效抑制效果,分別為EV71感染控制組之55~58% (p < 0.01)。1.2 µg/mL之甘草醇能夠抑制NF-κB進入細胞,為EV71感染控制組之48% (p < 0.05)。因此下游發炎物質COX-2蛋白表現被抑制,interleukin-1β (IL-1β) 及interleukin-6 (IL-6) 之含量亦受到抑制,分別為EV71感染控制組之66、72、89% (p < 0.05、p < 0.01 )。綜合本研究之結果,甘草醇具高度抑制EV71活性,主要作用機轉為直接致死病毒及抑制病毒之複製。於RD宿主細胞中,能夠抑制EV71所誘導NF-κB,能抑制RD宿主細胞內既有MAPK (p-p38、p-ERK1/2、p-JNK1/2/3) 家族之蛋白。因為NF-κB及MAPK受甘草醇之抑制,能有效減少下游發炎物質IL-1β、IL-6及COX-2等發炎物質生成,也因此減少病毒之子代生成及EV71之VP1及3C蛋白之表現,此抑制結果可以以防止發炎風暴的發生病造成中樞神經之傷害,肺水腫甚至造成患者死亡。
URI: http://hdl.handle.net/11455/96036
文章公開時間: 2020-08-24
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