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標題: Immunomodulatory and anti-inflammatory effects of camellia oil
作者: Chia-Ying Lin
關鍵字: 苦茶油
Camellia oil
primary cell
humoral immunity
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摘要: 苦茶油已被認為具有腸胃保健功效,然而目前苦茶油於免疫調節功效之研究甚少。本實驗利用體外 (in vitro) 及體內 (in vivo) 等實驗模式,評估苦茶油對 RAW 264.7 巨噬細胞株、初代免疫細胞及 BALB/c 小鼠免疫調節功能之影響。實驗結果顯示,苦茶油濃度高於 25 µg/mL 能有效促進巨噬細胞株的吞噬活性、有效抑制 NO 發炎媒介物分泌量及有效抑制IFN-γ 和 IL-6 促發炎激素分泌量之效果。在初代免疫細胞方面,苦茶油濃度高於 25 µg/mL 即可有效促使脾臟細胞增生、有效促進腹腔巨噬細胞之吞噬活性、有效抑制脾臟 IL-1β、IFN-γ (Con A刺激下) 促發炎細胞激素及 NO 發炎媒介物 (LPS刺激下) 分泌量、有效提升脾臟 IL-10及 IL-4 (苦茶油濃度高於 50 µg/mL 及 Con A刺激下) 抗發炎細胞激素分泌量、有效抑制培氏囊細胞 IL-1β (LPS刺激下) 及 IFN-γ 促發炎細胞激素及 NO 發炎媒介物分泌量,以及使脾臟淋巴球傾向CD19+ 之體液免疫分化。動物實驗方面,管餵 2 mL/kg b.w 苦茶油樣品 H 能有效促進脾臟細胞增生活性;管餵 2 mL/kg b.w 苦茶油樣品 H 及 L 均能有效抑制 IFN-γ (LPS刺激下) 及 NO 發炎媒介物 (LPS刺激下) 分泌量、有效促進 IL-10 及 IL-4 (Con A 或 LPS 刺激下) 抗發炎細胞激素分泌量,並同時具有促進體液免疫和細胞免疫 (Con A刺激下) 的效果。總結,苦茶油同時具有促進巨噬細胞吞噬活性、促進體液免疫反應、促進細胞免疫反應、調節 Th1/Th2 細胞激素分泌及抗發炎效果,並且在體外實驗以濃度為 200及 400 µg/mL之苦茶油樣品效果最佳,因此證實苦茶油具免疫調節功效及免疫調節保健品開發之潛力。
The beneficial effects of Camellia oil (Camellia oleifera Abel.) on gastrointestinal tract have been recognized; however, the effect of camellia oil on immunomodulatory has not been studied. Therefore, the aim of this study was to evaluate the immunomodulatory effects of camellia oil using an in vitro and in vivo models. In vitro, the results showed that RAW 264.7 macrophage treated with camellia oil (more than 25 µg/mL) significantly increased the phagocytosis activity, and reduced the productions of inflammatory cytokines, including NO, IFN-γ and IL-6. Treatment with camellia oil H (more than 25 µg/mL) significantly increased the proliferation to splenocytes and the phagocytosis activity of macrophage, and reduced the productions of inflammatory cytokines, including NO (with lipopolysaccharide), IL-1β and IFN-γ (with concanavalin A) from splenocytes, and increased the productions of anti-inflammatory cytokines, including IL-10 and IL-4 (camellia oil more than 50 µg/mL and with Con A stimulated) from splenocytes, and reduced the production of inflammatory cytokines, including NO, IL-1β (with LPS) and IFN-γ from Peyer's Patches. In addition, camellia oil could promote the immune regulation toward CD19+ humoral immune response. In vivo, the results showed that oral gavage of BALB/c mice with camellia oil H and L (2 mL/Kg) for 21 days, not only significantly increased the proliferation of splenocytes and the productions of anti-inflammatory cytokines, including IL-10 and IL-4 (with LPS or ConA), and also reduced the production of inflammatory cytokines, including IFN-γ (with LPS) and NO (with LPS). Moreover, camellia oil induced the splenocytes toward humoral and cellular (with ConA) immunity at the same time. In conclusion, camellia oil has the potential to enhance immune function, including increased the phagocytosis by macrophages, humoral immunity and cellular immunity. Camellia oil also could modulate the Th1/Th2 cytokines production, suggesting that this agent can be used for modulation of the immune system and as anti-inflammatory agent. The best effect of camellia oil on immunomodulatory is camellia oil with the concentration of 200 and 400 μg/mL in vitro. Therefore, our results support the possible use of camellia oil as a dietary supplement toward immunomodulation.
文章公開時間: 2020-08-28
Appears in Collections:食品暨應用生物科技學系



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