Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/96237
標題: Isolation and characterization of bacteria associated with tomato (Solanum lycopersicum L.) leaves
番茄葉圈與葉內細菌之分離及特性研究
作者: Sheng-Chen Peng
彭聖宸
關鍵字: 番茄
葉圈
拮抗試驗
植物生長促進特性
碳源代謝潛勢
tomato
phyllosphere
antagonistic test
plant growth promoting characteristics
carbon catabolic potential
引用: 吳文希。1990。植物病理學。茂昌圖書有限公司。台北。 周浩平和陳以錚。2016。農政與農情第283期。行政院農業委員會。台北。 柯勇。1998。作物病害與防治。藝軒圖書出版社。台北。 陳正次。 2000。 番茄栽培管理 果菜類蔬菜栽培斑課程講義。行政院農業試驗所。 台中。 許秀惠和安寶貞。2005。台灣農家要覽農作篇(三)。第三版。行政院農業委員會。台北。 陽偉正。1992。台灣地區現有作物栽培品種名錄《茄科篇》。行政院農業試驗所。台中。 費雯綺和王喻其。2007。植物保護手冊:蔬菜篇。農委會藥毒所。台中。 黃郁瑄。2014。兼性少樣分營細菌之分離、營養及植物生長特性研究。國立中興大學土壤環境科學系碩士論文。台中。 楊秋忠。2010。土壤與肥料。第九版。農世股份有限公司。台中。 Abril, A.B., P.A. Torres, and E.H. Bucher. 2005. The importance of phyllosphere microbial populations in nitrogen cycling in the Chaco semi-arid woodland. J. Trop. Eco. 21:103-107. Abril, G., H. Etcheber, and B. Delilleetal. 2003. Carbonate dissolution in the turbid and eutrophic Loire estuary. Mar. Ecol. Prog. Ser. 259:129–138. Alejandro, P.G., D. Romero, and A.D. Vicente. 2011. Plant protection and growth stimulation by microorganisms: biotechnological applications of Bacilli in agriculture. Curr. Opin. Biotechnol. 22: 187-193. Ali, S.S., and N.N. Vidhale. 2013. Bacterial siderophore and their application: A review. Int. J. Curr. Microbiol. Appl. Sci. 2: 303-312. Andrews, J.H., and R. F. Harris. 2000. The ecology and biogeography of microorganisms on plant surface. Annu. Rev. Phytopathol. 38:145-180. Angus, A.A., A. Lee, M.R. Lum, M. Shehayeb, R. Hessabi, N.A. Fujishige, S. Yerrapragada, S. Kano, N. Song, P. Yang, P.E. de los Santos, S.M. de Faria, F.D. Dakora, G. Weinstock, and A.M. Hirsch. 2013. Nodulation and effective nitrogen fixation of Macroptilium atropurpureum (siratro) by Burkholderia tuberum, a nodulating and plant growth promoting beta-proteobacterium, are influenced by environmental factors. Plant Soil. 369: 543–562. Babu-Khan, S., C.T. Yeo, W.L. Martin, M.R. Duron, R.D. Rogers, and A. Goldstein. 1995. Cloning of a mineral phosphate-solubilizing gene from Pseudomonas cepacia. Appl. Environ. Microbiol. 61:972–978. Balinte, P., S.J. Simmons, J.E. Blum, C.L. Ballare, and A.E. Stapleton. 2010. Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection. Mol. Plant Microbe Inter. 23: 473–484. Banerjee, M., and L. Yasmin. 2002. Sulfur oxidizing rhizobacteria: an innovative environment friendly soil biotechnological tool for better canola production. Proceeding Agroenviron: 1–7. Barney, B.M., H.I. Lee, P.C.D. Santos, B.M. Hoffman, D.R. Dean, and L.C. Seefeldt. 2005. Breaking the N2 triple bond: insights into the nitrogenase mechanism. Dalton Trans. 35:2277-2284. Bartel, B., and G.R. Fink. 1994. Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana. Proc. Natl. Acad. Sci. U.S.A. 91: 6649–6653. Bashan, Y., and G. Holgui. 1997. Azospirillum-plant relationships: Environmental and physiological advances (1990–1996). Can. J. Microbiol. 43: 103–121. Beattie, G.A., and S. E. Lindow. 1995. The secret life of foliar bacterial pathogens on leaves. Annu. Rev. Phytopathol. 33:145-172. Berg, G. 2009. Plant-microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Appl. Microbiol. Biotechnol. 84: 11–18. Boelen, P., M.K. de Boer, G.W. Kraay, M.J.W. Veldhuis, and A.G.J. Buma. 2000. UV-BR induced DNA damage in natural marine picoplankton assemblages in the tropical Atlantic Ocean. Mar. Ecol. Prog. Ser. 193:1-9. Bonas, U., G. Van den Ackerveken, D. Büttner, K. Hahn, E. Marois, D. Nennstiel, L. Noel, O. Rossier, and B.Szurek. 2000. How the bacterial plant pathogen Xanthomonas campestris pv. vesicatoria conquers the host. Mol. Plant Pathol. 1: 73-76. Braun, S.D., J. Hofmann, A. Wensing, H. Weingart, M.S. Ullrich, and D. Spiteller. 2010. In vitro antibiosis by Pseudomonas syringae Pss22d, acting against the bacterial blight pathogen of soybean plants, does not influence in planta biocontrol. J. Phytopathol. 158: 288–295. Brom, S., L. Girard, C. Tun-Garrido, A. Garcia-de-los Santos, and P. Bustos. 2004. Transfer of the symbiotic plasmid of Rhizobium etli CFN42 requires cointegration with p42a, which may be mediated by site-specific recombination. J. Bacteriol. 186: 7538–7548. Cabrefiga, J., A. Bonaterra, and E. Montesinos. 2007. Mechanisms of antagonism of Pseudomonas fluorescens EPS62e against Erwinia amylovora, the causal agent of fire blight. Int. Microbiol. 10: 123–132. Camerini, S., B. Senatore, E. Lonardo, E. Imperlini, C. Bianco, G. Moschetti, G.L. Rotino, B. Campion, and R. Defez. 2008. Introduction of a novel pathway for IAA biosynthesis to rhizobia alters vetch root nodule development. Arch. Microbiol. 190: 67–77. Cassana, F., D. Perriga, V. Sgroya, O. Masciarellia, C. Pennab, and V. Lunaa. 2009. Azospirillum brasilense and Bradyrhizobium japonicum, inoculated singly or in combination, promote seed germination and early seeding growth in corn (Zea mays L) and soybean. Europ. J. Soil Biol. 45:28-35. Castagno, L.N., M.J. Estrella, A.I. Sannazzaro, A.E. Grassano, and O.A. Ruiz. 2011. Phosphate-solubilization mechanism and in vitro plant growth promotion activity mediated by Pantoea eucalypti isolated from Lotus tenuis rhizosphere in the Salado River Basin (Argentina). J. Appl. Microbiol. 110:1151-1165. Cattelan, A.J., P.G. Hartel, and J.J. Fuhrmann. 1999. Screening for plant growth-promoting rhizobacteria to promote early soybean growth. Soil Sci. Soc. Am. J. 63:1670–1680. Chabot, R., H. Antoun, and P.M. Cescas. 1996. Growth promotion of maize and lettuce by phosphate solubilizing Rhizobium leguminosarum biovar. Phaseoli. Plant Soil. 184:311-321. Chandrasekaran, M., and S.C. Chun. 2016. Induction of defence-related enzymes in tomato (Solanum lycopersicum) plants treated with Bacillus subtilis CBR05 against Xanthomonas campestris pv. vesicatoria. Biocontrol Sci. Techn. 26: 1366-1378. Chen, H., L. Wang, C.X. Su, G.H. Gong, P. Wang, and Z.L. Yu. 2008. Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis. Lett. Appl. Microbiol. 47:180-186. Chen, Y.P., P.D. Rekha, A.B. Arun, F.T. Shen, W.A. Lai, and C.C. Young. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl. Soil Ecol. 34: 33–41. Cimmino, A., A. Andolfi, G. Marchi, G. Surico, and A. Evidente. 2006. Phytohormone production by strains of Pantoea agglomerans from knots on olive plants caused by Pseudomonas savastanoi pv. Savastanoi. Phytopathol. Mediterr. 45:247-252. Clark, E., S. Manulis, Y. Ophir, I. Barash, and Y. Gafni. 1993. Cloning and characterization of iaaM and iaaH from Erwinia herbicola pathovar gypsophilae. Phytopathology. 83: 234–240. Classen, A.T., S.I. Boyle, K.E. Haskins, S.T. Overby, and S.C. hart. 2003. Community-level physiological profiles of bacteria and fungi: plate type and incubation temperature influences on contrasting soils. FEMS Microbiol. Ecol. 44: 319-328. Crespol, J.M., J.L. Boiardi1, and M.F. Lunal. 2011. Mineral phosphate solubilization activity of Gluconacetobacter diazotrophicus under P-limitation and plant root environment. Agricultural Science 2:16-22. Compant, S., B. Duffy, J. Nowak, C. Clément, and E. Ait Barka. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action and future prospects. Appl. Environ. Microbiol. 71: 4951–4959. Daound, Z., M. Sura, and R.M. Abdel-Massih. 2013. Pectin shows antibacterial activity against Helicobacter pylori. Adv. Biosci. Biotechnol. 4:273-277. Delmotte, N., C. Knief, S. Chaffron, G. Innerebner, B. Roschitzki, R. Schlapbach, C. Mering, and J.A. Vorholt. 2009. Community proteogenomics reveals insights into the physiology of phyllosphere bacteria. Pro. Natl. Aca. Sci. USA. 106: 16428–16433. Dobranic, J.K., and J.C. Zak. 1999. A microtiter plate procedure for evaluating fungal functional diversity. Mycologia. 91: 756. Duca, D., J. Lorv, C.L. Patten, D. Rose, and B.R. Glick. 2014. Indole-3-acetic acid in plant-microbe interactions. Antonie Van Leeuwenhoek Int. J. Gen. Mol. Microbiol. 106: 85–125. Eckert, B., O.B. Weber, G. Kirchhof, A. Halbritter, M. Stoffels, and A. Hartmann. 2001. Azospirillum doebereinerae sp. nov., a nitrogen-fixing bacterium associated with the C4-grass miscanthus. Int. J. Syst. Evol. Microbiol. 51: 17–26. Edwards, U., T. Rogall, H. Blöcker, M. Emde, and E.C. Böttger. 1989. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res. 17: 7843–7853. Egamberdieva, D. 2008. Plant growth promoting properties of rhizobacteria isolated from wheat and pea grown in loamy sand soil. Turk. J. Biol. 32:9-15. Egamberdieva, D. 2010. Growth response of wheat cultivars to bacterial inoculation in calcareous soil. Plant Soil Environ. 56:570-573. Enya, J., H. Shinohara, S. Yoshida, T. Tsukiboshi, H. Negishi, K. Suyama, and S. Tsushima. 2007. Culturable leaf-associated bacteria on tomato plants and their potential as biological control agents. Microb. Ecol. 53:524-536. Farzana, Y., and O. Radizah. 2005. Influence of rhizobacterial inoculation on growth of the sweet potato cultivar. OnLine J. Biol. Sci. 1:176-179. Finkel, O.M., A.Y. Burch, S.E. Lindow, A.F. Post, and S. Belkin. 2011. Geographical location determines the population structure in phyllosphere microbial communities of a salt-excreting desert tree. Appl. Environ. Microbiol. 77:7647-7655. Fuentes-Ramirez, L.E., T. Jimenez-Salgado, I.R. Abarca-Ocampo, and J. Caballero-Mellado. 1993. Acetobacter diazotrophicus, an indoleacetic acid producing bacterium isolated from sugarcane cultivars of México. Plant Soil 154: 145–150. Glick, B.R. 2003. Phytoremediation: synergistic use of plants and bacteria to clean up the environment. Biotechnol. Adv. 21: 383–393. Glick, B.R., D.M. Karaturovic, and P.C. Newell. 1999. A novel for rapid isolation of plant growth promoting Pseudomonas. Can. J. Microbiol. 41:533-536. Godfray, H.C., R. Beddington, I.R. Crute, L. Haddad, D. Lawrence, J.F. Muir, J. Pretty, S.Robinson, S.M. Thomas, and C. Toulmin. 2010. Food security: The challenge of feeding 9 billion people. Science 327:812-818. Goldstein, A.H. 1995. Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilization by gram negative bacteria. Biol. Agric. Hortic. Int. J. Sustain. Prod. Syst. 12: 185–193. Gulati, A., P. Rahi, and P. Vyas. 2008. Characterization of phosphate-solubilizing fluorescent pseudomonads from the rhizosphere of seabuckthorn growing in the cold deserts of Himalayas. Curr. Microbiol. 56:73-79. Gyaneshwar P., L.J. Parekh, G. Archana, P.S. Poole, M.D. Collins, R.A. Hutson, and G.N. Kumar. 1999. Involvement of a phosphate starvation inducible glucose dehydrogenase in soil phosphate solubilization by Enterobacter asburiae. FEMS Microbiol. Lett. 171:223-229. Halder, A.K., and P.K. Chakrabartty. 1993. Solubilization of inorganic phosphate by Rhizobium. Folia. Microbiol. 38:325-330. Hazra, F., and E. Pratiwi. 2013. Isolation, characterization, and molecular identification of phosphate solubilizing bacteria from several tropical soils. J. Trop. Soils. 18:67-74. Hemambika B., V. Balasubramanian, V.R. Kannan, and R.A. James. 2013. Screening of chromium-resistant bacteria for plant growth-promoting activities. Soil Sediment. Contam. 22:717-736. Hirano, K., M. Hayatsu, I. Nioh, and H. Nakai. 2001. Comparison of nitrogen-fixing bacterial flora of rice rhizosphere in the fields treated long-term with agrochemicals and non-agrochemicals. Microbes Environ. 16:155-160. Hoagland, D.R., and D.I. Arnon. 1950. The water-culture method for growing plants without soil. Circular. California Agricultural Experiment Station 347. Huber, D.M., and N.S. Wilhelm. 1988. The role of manganese in resistance to plant disease.In: Graham RD, Hannam RJ, Uren NC (Eds.) Manganese in Soils and Plants. Springer, Dordrecht. 155-173. Idris, R., R. Trifonova, M. Puschenreiter, W.W. Wenzel, and A. Sessitsch. 2004. Bacterial communities associated with flowering plants of the Ni hyper accumulator Thlaspi goesingense. Appl. Environ. Microbiol .70: 2667–2677. Isla, M.I., R.M. Ordóñez, M.I. Moreno, A.R. Sampietro, and M.A. Vattuone. 2002. Inhibition of hydrolytic enzyme activities and plant pathogen growth by invertase inhibitors. J. Enzyme Med. Chem. 17: 37-43. Jackson, E.F., H.L. Echlin, and C.R. Jackson. 2006. Changes in the phyllosphere community of the resurrection fern, Polypodium polypodioides, associated with rainfall and wetting. FEMS. Microbiol. Ecol. 58: 236–246. Jameson, P.E. 2000.Cytokinins and auxins in plant-pathogen interactions - An overview. Plant Growth Reg. 32: 369-380. Jha, P., and A. Kumar. 2009. Characterization of novel plant growth promoting endophytic bacterium Achromobacter xylosoxidans from wheat plant. Microb. Ecol. 58: 179–188. Jiménez-Zurdo, J. I., P.F. Mateos, F.B. Dazzo, and E. Martinez-Molina. 1996. Cell-bound cellulase and polygalacturonase production by Rhizobium and Bradyrhizobium species. Soil Biol. Biochem. 28:917-921. Joseph S., and M.S. Jisha. 2009. Buffering reduces phosphate solubilizing ability of selected strains of bacteria. World J. Agric. Sci. 5: 135-137. Junaid, J.M., N.A. Dar, T.A. Bhat, A.H. Bhat, and M.A. Bhat. 2013. Commercial biocontrol agents and their mechanism of action in the management of plant pathogens. Int. J. Mod. Plant Anim. Sci. 1:39-57. Kadivar, H., and A.E. Stapleton. 2006. Ultraviolet radiation alters maize phyllosphere bacterial diversity. Microbial. Ecol. 45:353–361. Kang, S.M., R. Radhakrishnan, Y.H. You, G.J. Joo, I.J. Lee, K.E. Lee, and J.H. Kim. 2014. Phosphate solubilizing Bacillus megaterium mj1212 regulates endogenous plant carbohydrates and amino acids contents to promote mustard plant growth. Indian J. Microbiol. 54:427-433. Kenarova, A., G. Radeva, I. Traykov, and S. Boteva. 2014. Community level physiological profiles of bacterial communities inhabiting uranium mining impacted sites. Ecotoxicol. Environ. Saf. 100: 226-232. Kennedy I.R., A.T.M.A. Choudhury, and M.L. Kecskés. 2004. Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited?. Soil Biol. Biochem. 36:1229–1244. Khan, K.S., and R.G. Joergensen. 2009. Changes in microbial biomass and P fractions in biogenic household waste compost amended with inorganic P fertilizers. Bioresour. Technol. 100: 303–309. Kirkpatrick, F.H. 1990. Overview of agarose gel properties. Cell Mol. Biol. 1: 9–22. Kloepper, J.W., J. Leong, M. Teinize, and M.N. Schroth. 1980. Enhanced plant growth by siderophores produced by plant growth promoting rhizobacteria. Nature 286: 885-886. Knief, C., N. Delmotte, S. Chaffron, M. Stark, G. Innerebner, R. Wassmann, C. Mering, and J.A. Vorholt. 2012. Metaproteogenomic analysis of microbial communities in the phyllosphere and rhizosphere of rice. ISME J. 6: 1378–1390. Knief, C., V. Dengler, P.L.E. Bodelier, and J.A. Vorholt. 2012. Characterization of Methylobacterium strains isolated from the phyllosphere and description of Methylobacterium longum sp. nov. Antonie van Leeuwenhoek. 101:169-183. Kobayashi, M., and S. Shimizu. 1994. Versatile nitrilases: Nitrile-hydrolysing enzymes. FEMS Microbiol. Lett. 120:217–223. Kpomblekou, K., and M.A. Tabatabai. 1994. Effect of organic acids on release of phosphorus from phosphate rocks1. Soil Sci. 158:442-453. Krishnaraj, P.U., and S. Dahale. 2014. Mineral phosphate solubilization: Concepts and properties in sustainable agriculture. Proc. Indian Nath. Sci. Acad. 80: 389-405. Kumar, V., and N. Narula. 1999. Solubilization of inorganic phosphates and growth emergence of wheat as affected by Azotobacter chroococcum mutants. Biol. Fertil. Soils. 28: 301–305. Labarca, C., and K. Paigen. 1980. A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102: 344–352. Lambais, M.R., D.E. Crowley, J.C. Cury, R.C. Bull, and R.R. Rodrigues. 2006. Bacterial diversity in tree canopies of the Atlantic forest. Science 312: 1917. Leinhos, V., and O. Vacek. 1994. Biosynthesis of auxins by phosphate-solubilizing rhizobacteria from wheat (Triticum aestivum) and rye (Secale cereale). Microbiol. Res. 149:31–35. Loper, J., and M. Schroth. 1986. Influence of bacterial sources of indole-3-acetic-acid on root elongation of sugar-beet. Phytopathology. 76:386–389. Loper, J.E., and M.D. Henkels. 1999. Utilization of heterologous siderphores enhances levels of iron available to Pseudomonas putida in the rhizosphere. Appl. Environ. Microbiol. 65: 5357-5363. Madhaiyan, M., S. Poonguzhali, H.S. Lee, K. Hari, S.P. Sundaram, and T.M. Sa. 2005. Pink-pigmented facultative methylotrophic bacteria accelerate germination, growth and yield of sugarcane clone Co86032 (Saccharum officinarum L.). Biol. Fert. Soils 41: 350–358. Madhaiyan, M., S. Poonguzhali, M. Senthikumar, J.S. Lee, and K.C. Lee. 2012. Methylobacterium gossipiicola sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the cotton phyllosphere. Int. J. Syst. Evol. Microbiol. 62:162-167. Madhaiyan, M., S. Poonguzhali, S.W. Kwon, and T.M. Sa. 2009. Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice. Int. J. Syst. Evol. Microbiol. 59:22-27. Malfanova, N.V. 2013. Endophytic bacteria with plant growth promoting and biocontrol abilities. Doctoral Thesis. Leiden University. 166. Malviya, J., and K. Singh. 2012. Characterization of novel plant growth promoting and biocontrol strains of fluorescent Pseudomonas for crop. Int. J. Med. Res. 1:235-244. Mardad, I., A. Serrano, and A. Soukri. 2013. Solubilization of inorganic phosphate and production of organic acids by bacteria isolated from a Moroccan mineral phosphate deposit. Afr. J. Microbiol. Res. 7:626-635. Marlatt, M.L., J.C. Correll, P. Kaufmann and P.E. Cooper. 1996. Two genetically distinct populations of Fusarium oxysporum f. sp. lycopersici race 3 in the Unites States. Plant. Dis. 80:1336-1342. Marlow, J.L., and T. Kosuge. 1972. Tryptophan and indoleacetic acid transport in the olive and oleander knot organism Pseudomonas savastanoi (E. F. Smith) Stevens. J. Gen. Microbiol. 72:211-219. Matsuoka, H., M. Akiyama, K. Kobayashi, and K. Yamaji. 2013. Fe and P solubilization under limiting conditions by bacteria isolated from Carex kobomugi roots at the Hasaki coast. Curr. Microbiol. 66: 314–321. Mc Loughlin, T., J. Quinn, A. Bettermann, and R. Bookland. 1992. Pseudomonas cepacia suppression of sunflower wilt fungus and role of antifungal compounds in controlling the disease. Appl. Environ. Microbio. 58:1760-1763. Messenger, A.J.M., and C. Ratledge. 1985. Siderophores. In: Young MM (Ed.) Comprehensive Biotechnology. Pergamon press, New York. 275-295. Milagres, A.M.F., A. Machuca, and D. Napoleão. 1999. Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay. J. Microbiol. Methods 37:1-6. Mitchell, D.L., and D. Karentz. 1993. The induction and repair of DNA photo damage in the environment. In: Young AR, Moan J, Björn LO, and Nultsch W (Eds.) Environmental UV-B Photobiology. Springer, Boston, MA. 345-377. Mole, B.M., D.A. Baltrus, J.L. Dangl, and S.R. Grant. 2007. Global virulence regulation networks in phytopathogenic bacteria. Trends Microbiol. 15: 363–371. Monteiro, L., R.L.R. Mariano, and A.M. Souto-Maior. 2005. Antagonism of Bacillus spp. against Xanthomonas campestris pv. campestris. Braz. Arch. Biol. Technol. 48:23-29. Morris, R.O. 1995. Genes specifying auxin and cytokinin biosynthesis in prokaryotes. In: Davies PJ (Ed.) Plant hormones. Kluwer Academic. 318-339. Moulin, L., A. Munive, B. Dreyfus, and C. Boivin-Masson. 2001. Nodulation of legumes by members of the beta-subclass of Proteobacteria. Nature 411: 948–950. Nakamiya, K., T. Nakayama, H. Ito, Y. Shibata, and M. Morita. 2009. Isolation and properties of 2-chlorovinyl arsenic acid degrading microorganism. J. Haz. Mat. 165:388–393. Nautiyal, C.S. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. Fed. Eur. Microbiol. Soc. 170: 265–270. Oberhänsli, T., G. Défago, and D. Haas. 1991. Indole-3-acetic-acid (IAA) synthesis in the biocontrol strain CHA0 of Pseudomonas fluorescens—Role of tryptophan side-chain oxidase. J. Gen. Microbiol. 137: 2273–2279. Obradovic, A., J.B. Jones, M.T. Momol, S.M. Olson, L.E. Jackson, B. Balogh, and F.B. Iriarte. 2005. Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato. Plant Disease 89:712-716. Ogut, M., F. Er, and N. Kandemir. 2010. Phosphate solubilization potentials of Acinetobacter strains and their relations with soil properties. Eurasian Chem.-Technol. J. 12:231-239. Ogut, M., F. Er, and N. Kandemir. 2010. Phosphate solubilization potentials of soil Acinetobacter strains. Biol. Fertil. Soils. 46:707-715. Omer, Z.S., R. Tombolini, A. Broberg, and B. Gerhardson. 2004. Indole-3-acetic acid production by pink-pigmented facultative methylotrophic bacteria. Plant Growth Reg. 43: 93–96. Osborne, W.J., V.S. Saravanan, A. Mukherjee, and N. Chandrasekaran. 2010. Impact of Vetiveria zizanioides rhizosphere bacterial isolates on PGPR traits and cadmium resistance. J. Ecobiotechnol. 2:34-42. Oteino, N., R.D. Lally, S. Kiwanuka, A. Lloyd, D. Ryan, K.J. Germaine, and D.N. Dowling. 2015. Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates. Front. Microbiol. 6:745. Pal, K.K., K. Tilak, A.K. Saxcna, R. Dey, and C.S. Singh. 2001. Suppression of maize root diseases caused by Macrophomina phaseolina, Fusarium moniliforme and Fusarium graminearum by plant growth promoting rhizobacteria. Microbiol. Res. 156: 209–223. Panagopoulos, G.N., P.D. Megaloikonomos, M. Liontos, E. Giannitsioti, M. Drogari-Apiranthitou, A.F. Mavrogenis, and V. Kontogeorgakos. 2016. Pseudomonas oryzihabitans infected total hip arthroplasty. J. Bone Jt. Infect. 1:54-58. Passari, A.K., V.K. Mishra, V.V. Leo, V.K. Gupta, and B.P. Singh. 2016. Phytohormone production endowed with antagonistic potential and plant growth promoting abilities of culturable endophytic bacteria isolated from Clerodendrum colebrookianum Walp. Microbiol. Res. 193: 57–73. Patel, D.K., G. Archana, and G.N. Kumar. 2008. Variation in the nature of organic acid secretion and mineral phosphate solubilization by Citrobacter sp. DHRSS in the presence of different sugars. Curr. Microbiol. 56:168-174. Patten, C.L., and B.R. Glick. 1996. Bacterial biosynthesis on indole-3-acetic acid. Can. J. Microbiol. 42: 207–220. Pérez, E., M. Sulbarán, M.M. Ball, and L.A. Yarzábal. 2007. Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biol. Biochem. 39: 2905–2914. Pérez-Miranda, S., N. Cabirol, R. George-Téllez, L.S. Zamudio-Rivera, and F.J. Fernández. 2007. O-CAS, a fast and universal method for siderophore detection. J. Microbiol. Methods. 70:127-131. Plante, A.F. 2006. Soil biogeochemical cycling of inorganic nutrients and metals. In: Paul EA (Ed.) Soil Microbiology, Ecology, and Biochemistry. Oxford, UK: Elsevier Acad. 389-430. Pliego, C., F. Kamilova, and B. Lugtenberg. 2011. Plant growth-promoting bacteria: Fundamentals and exploitation. In: Maheshwari DK (Ed.) Bacteria in Agrobiology: Crop Ecosystems. Springer Berlin Heidelberg. 295–343. Perotto, S., R. Peretto, A. Faccio, A. Schubert, A. Varma, and P. Bonfante. 1995. Ericoid mycorrhizal fungi: cellular and molecular bases of their interactions with the host plant. Can. J. Bot. 73:557-568. Popplestone, C., and A. Unran. 1973. Biosynthesis of lycomarasmin. Can. J. Chem. 51:3493-3949. Puente, M.E., Y. Bashan, C.Y. Li, and V.K. Lebsky. 2004. Microbial populations and activities in the rhizoplane of rock-weathering desert plants. Root colonisation and weathering of igneous rocks. Plant Biol. 6:629–642. Rasche, F., E. Marco-Noales, H. Velvis, L.S. van Overbeek, M.M. Lopez, J.D. van Elsas, and A. Sessitsch. 2006a. Structural characteristics and plant-beneficial effects of bacteria colonizing the shoots of field grown conventional and genetically modified T4-lysozyme producing potatoes. Plant Soil. 289:123–140. Rasche, F., R. Trondl, C. Naglreiter, T.G. Reichenauer, and A. Sessitsch. 2006b. Chilling and cultivar type affect the diversity of bacterial endophytes colonizing sweet pepper (Capsicum anuum L.). Can. J. Microbiol. 52:1036–1045. Rastogi, G., A. Sbodio, J.J. Tech, T.V. Suslow, G.L. Coaker, and J.H. Leveau. 2012. Leaf microbiota in an agroecosystem: spatio temporal variation in bacterial community composition on field-grown lettuce. ISME J. 6: 1812–1822. Reiter, B., and A. Sessitsch. 2006. Bacterial endophytes of the wild flower Crocus albiflorus analysed by characterisation of isolates and by a cultivation-independent approach. Can. J. Microbiol. 52:140–149. Roberts, T.L. 2009. The role of fertilizer in growing the world's food. Better Crops Plant Food. 93:12-15. Robertson, G.P., and P.M. Groffman. 2006. Nitrogen transformations. In: Paul EA (Ed.) Soil Microbiology, Ecology, and Biochemistry. Oxford, UK: Elsevier Acad. 341-362. Rodrı́guez, H., and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17: 319–339. Rogel, M.A., I. Hernandez-Lucas, L.D. Kuykendall, D.L. Balkwill, and E. Martinez-Romero. 2001. Nitrogen-fixing nodules with Ensifer adhaerens harboring Rhizobium tropici symbiotic plasmids. Appl. Environ. Microbiol. 67: 3264–3268. Ruggiero, C. E., M.P. Neu, J.H. Matonic, and S.D. Reilly. 2000. Interactions of Pu with desferrioxamine siderophores can affect bioavailability and mobility. Actinide Res. Q. 2000:16-18. Sadikot, R.T., T.S. Blackwell, J.W. Christman, and A.S. Prince. 2005. Pathogen–host interactions in Pseudomonas aeruginosa Pneumonia. Am. J. Crit. Care Med. 171: 1209-1223. Saile, E., J.A. McGarvey, M.A. Schell, and T.P. Denny. 1997. Role of extracellular polysaccharide and endoglucanase in root invasion and colonization of tomato plants by Ralstonia solanacearum. Phytopathology 87: 1264-1271. Sandhu, A., L.J. Halverson, and G.A. Beattie. 2007. Bacterial degradation of air borne phenol in the phyllosphere. Environ. Microbiol. 9: 383–392. Scherwinski, K., R. Grosch, and G. Berg. 2008. Effect of bacterial antagonists on lettuce: active biocontrol of Rhizoctonia solani and negligible, short-term effects on non-target microorganisms. FEMS Microbiol. Ecol. 64: 106-16. Scheublin, T.R., and J.H. Leveau. 2013. Isolation of Arthrobacter species from the phyllosphere and demonstration of their epiphytic fitness. Microbiol. 2: 205–213. Schuch, R., D. Nelson, and V.A. Fichetti. 2002. A bacteriolytic agent that detects and kills Bacillus anthracis. Nature 418: 884-889. Sekine, M., K. Watanabe, and K. Syono. 1989. Molecular cloning of a gene for indole-3-acetamide hydrolase from Bradyrhizobium japonicum. J. Bacteriol. 171: 1718–1724. Shen, F.T., and C.C. Young. 2005. Rapid detection and identification of the metabolically diverse genus Gordonia by 16S rRNA-gene-targeted genus-specific primers. FEMS Microbiol. Lett. 250: 221–227. Silini-Cherif, H., A. Silini, and S. Yadav. 2012. Isolation and characterization of plant growth promoting traits of a rhizobacteria: Pantoea agglomerans lma2. Pak. J. Biol. Sci. 15:267-276. Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 509–517. Spaepen S., J. Vanderleyden, and R. Remans. 2007 Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol. Rev. 31: 425–448. Spaepen, S., and J. Vanderleyden. 2011. Auxin and Plant-Microbe Interactions. Cold Spring Harbor Perspect. Biol. 3:a001438. Sridevi, M., and K.V. Mallaiah. 2009. Phosphate solubilization by Rhizobium strains. Indian J. Microbiol. 49:98-102. Stackebrandt, E. 2002. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int. J. Syst. Evol. Microbiol. 52: 1043–1047. Sulbarán, M., E. Pérez, M.M. Ball, A. Bahsas, and L.A. Yarzábal. 2009. Characterization of the mineral phosphate-solubilizing activity of Pantoea aglomerans MMB051 isolated from an iron-rich soil in southeastern Venezuela (Bolívar State). Curr. Microbiol. 58:378-383. Teixeira, D.A., A.C. Alfenas, R.G. Mafia, E.M. Ferreira, L.D. Siqueira, A. Luiz, L.A. Maffia, and A.H. Mounteer. 2007. Rhizobacterial promotion of eucalypt rooting and growth. Braz. J. Microbiol. 38:118-123. Thakur, B.R., R.K. Singh, and A.K. Handa. 1997. Chemistry and uses of pectin – A review. Crit. Rev. Food Sci. Nutr. 37:47-73. Theunis, M., H. Kobayashi, W.J. Broughton, and E. Prinsen. 2004. Flavonoids, NodD1, NodD2, and nod-box NB15 modulate expression of the y4wEFG locus that is required for indole-3-acetic acid synthesis in Rhizobium sp. strain NGR234. Mol. Plant-Microbe. Interact. 17: 1153–1161. Tilak, K.V.B.R., N. Ranganayaki, K.K. Pal, R. De, A.K. Saxena, C.S. Nautiyal, S. Mittal, A.K. Tripathi, and B.N. Tohri. 2005. Diversity of plant growth and soil health supporting bacteria. Curr. Sci. 89:136–150. Tripura, C., P.S. Reddy, M.K. Reddy, B. Sashidhar, and A.R. Podile. 2007. Glucose dehydrogenase of a rhizobacterial strain of Enterobacter asburiae involved in mineral phosphate solubilization shares properties and sequence homology with other members of enterobacteriaceae. Indian J. Microbiol. 47:126-131. Vance, C.P. 2001. Symbiotic nitrogen fixation and phosphorus acquisition: plant nutrition in a world of declining renewable resources. Plant Physiol. 127: 390–397. Vassilev, N., A. Medina, and M. Vassileva. 2006. Microbial solubilization of rock phosphate on media containing agro-industrial wastes and effect of the resulting products on plant growth and P uptake. Plant Soil. 287:77-84. Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255: 571-586. Viruel, E., M.E. Lucca, and F. Siñeriz. 2011. Plant growth promotion traits of phosphobacteria isolated from Puna, Argentina. Arch. Microbiol. 193: 489–496. Von Gunten, H.R. and P. Benes. 1995. Speciation of radionuclides in the environment. Radiochim. Acta. 69:1-29. Vorholt, J.A. 2012. Microbial life in the phyllosphere. Nat. Rev. Microbiol. 10: 828-840. Walpola, B.C., and M.H. Yoon. 2013. Isolation and characterization of phosphate solubilizing bacteria and their co-inoculation efficiency on tomato plant growth and phosphorous uptake. Afr. J. Microbiol. Res. 7: 266–275. Walpola, B.C., and M.H. Yoon. 2013. Isolation and characterization of phosphate solubilizing bacteria and their co-inoculation efficiency on tomato plant growth and phosphorous uptake. Afr. J. Microbiol. Res. 7:266-275. Whipps, J.M., P. Hand, D. Pink, and G.D. Bending. 2008. Phyllosphere microbiology with special reference to diversity and plant genotype. J. Appl. Microbiol. 105: 1744-1755. Woodward, F.I., and M.R. Lomas. 2004. Vegetation dynamics – simulating responses to climatic change. Biol. Rev. 79: 643–670. Young, C.C., F.T. Shen, and S. Singh. 2012. Strategies for the exploitation and development of biofertilizer. In: Maheshwari DK (Ed.) Bacteria in Agrobiology: Plant Probiotics. Springer-Verlag Berlin Heidelberg.127-139. Yutthammo, C., N. Thongthammachat, P. Pinphanichakarn, and E. Luepromchai. 2010. Diversity and activity of PAH-degrading bacteria in the phyllosphere of ornamental plants. Microb. Eco. 59: 357–368. Zak, J.C., M.R. Willig, D.L. Moorhead, and H.G. Wildman. 1994. Functional diversity of microbial communities: A quantitative approach. Soil Biol. Biochem. 26: 1101-1108. Zhang, A., G. Zhao, T. Gao, W. Wang, J. Li, S. Zhang, and B. Zhu. 2013. Solubilization of insoluble potassium and phosphate by Paenibacillus kribensis CX-7: A soil microorganism with biological control potential. African. J. microbial. Res. 7:41-47. Zhang, B., Z. Bai, D. Hoefel, X. Wang, L. Zhang, and Z. Li. 2010. Microbial diversity within the phyllosphere of different vegetable species. In: Méndez-Vilas A(Ed.) Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Formatex Research Center. 1067-1077. Zhang, L., and R.G. Birch. 1997. Mechanisms of biocontrol by Pantoea dispersa of sugar cane leaf scald disease caused by Xanthomonas albilineans. J. Appl. Microbiol. 82:448-454. Zuniga, A., M.J. Poupin, R. Donoso, T. Ledger, N. Guiliani, R.A. Gutierrez, and B. Gonzalez. 2013. Quorum sensing and indole-3-acetic acid degradation play a role in colonization and plant growth promotion of Arabidopsis thaliana by Burkholderia phytofirmans PsJN. Mol. Plant-Microbe. Interactions. 26: 546-553.
摘要: 番茄是全球主要且受歡迎的蔬菜作物之一,其產量在蔬菜類作物中排行第四。但為了因應全球人口不斷提高,農民多使用化學肥料及農藥來維持或提高番茄的的產量,然而長期並過量的施用肥料及農藥讓農地的地力持續退化,對環境造成的問題難以評估。因此藉由農用微生物製劑的開發,減少化學肥料及農藥的使用量是熱門且重要研究方向。有別於過去於過去開發成農用微生物製劑時,菌株篩選來源來自於根圈土壤,本研究旨在從番茄葉圈及葉內中篩選出菌株,讓這些與番茄適應性良好的菌株,能夠實際用於番茄的種植上,並期望能達到減少施用肥料、農藥的目的。 本研究利用肉湯抽出物培養基從番茄葉圈中篩出44個分離株,利用甲醇培養基篩選出68個分離株,全部共112個分離株,經16S rDNA序列鑑定後,分別屬於3種菌門、4種菌綱,共有Acinetobacter, Bacillus, Brachybacterium, Exiguobacterium, Lysinibacillus, Methylobacterium, Microbacterium, Paenibacillus, Pantoea, Paracoccus, Pseudomonas, Rhizobium, Sphingomonas及Xanthomonas等14個菌屬。肉湯抽出物固體培養基篩選到數量最多的菌屬依序是Xanthomonas的 27%、Pseudomonas 的20%及Bacillus 的20%;甲基培養基篩選到數量最多的菌屬依序是Xanthomonas的 44%、Pseudomonas的 22%及Pantoea 的18%。從以上菌株中挑選出23個不同菌屬菌種的分離株,與番茄病原菌Xanthomonas campestri pv. vesicatoriar進行拮抗試驗,共有5個分離株對病原菌表現出拮抗圈。並從5個分離株中挑出2個拮抗病原菌的分離株Pantoea sp. MYEO 12及 Bacillus sp. NYPO 7進行番茄抗病的生物試驗,將番茄植株種植在生長箱中無菌的環境下,接種病原菌及NYPO 7的處理,能有效將番茄的罹病指數從4.3降到2.4,而接種病原菌及MYEO 12的處理,番茄的罹病指數仍維持在3.8,無法有效防治病原菌,但是無論是接種MYEO 12及NYPO 7都能有效提高番茄幼苗的生質量,幫助其生長。接著對葉圈分離株進行植物生長促進能力的分析試驗,在游離固氮活性及溶磷活性試驗中,分別各有5個分離株表現較佳的活性,其固氮活性介於0.136-0.456 ηmol/(tube x hr);溶磷能力介於225-1430 μg/mL;在IAA合成能力的試驗中,23個分離株都表現出IAA的合成能力,且IAA濃度高於50 μg/mL的菌株有6株,可以推測葉圈分離株主要藉由合成IAA來促進植物生長。從以上試驗中,挑出6個具有植物生長促進潛力的分離株Lysinibacillus sp. NYPO 3、Bacillus sp. NYPO 7、Methylobacterium sp. MYET 17、Microbacterium sp. MYEO 1、Pantoea sp. MYEO 12及Rhizobium sp. MYEO 9進行番茄溫室的盆栽試驗。雖然接種NYPO 3的盆栽處理在乾重上能高於對照組,接種MYEO 1及接種MYEO 12的盆栽處理在株高上也高於對照組,但都沒有顯著的提高。另外對於番茄盆栽微生物群落的碳代謝潛勢與群落結構分析,結果顯示接種不同的菌株,對番茄盆栽介質的菌相有明顯的改變,但菌相的改變對於番茄植株而言沒有顯著的影響。
In response to the increasing population around the world, food production largely relies on the application of chemical fertilizers and chemical pesticides. However, the high amount of agrochemical input has led to many environmental problems such as soil degradation and ecological imbalance. Development of microbial agents used to increase nutrient availability or control plant pathogen provides alternatives to sustain plant growth and health. Plant rhizosphere and phyllosphere have been demonstrated to harbor highly diverse microorganisms with various functions. However, our knowledge of the microbiology of phyllospheric bacteria has historically lagged behind our knowledge of the microbiology of rhizospheric bacteria. This motivates us to isolate and characterize bacteria associated with leaves of tomato, the most significant horticultural crop worldwide. In the present study a total of 44 and 68 isolates were obtained from phyllosphere of tomato using nutrient agar and methanol medium agar, respectively. 16S rDNA sequences assigned them to 14 genera namely Acinetobacter, Bacillus, Brachybacterium, Exiguobacterium, Lysinibacillus, Methylobacterium, Microbacterium, Paenibacillus, Pantoea, Paracoccus, Pseudomonas, Rhizobium, Sphingomonas and Xanthomonas. Among them, genera Xanthomonas and Pseudomonas were the dominant bacterial groups obtained from phyllosphere no matter which medium was used for isolation. Twenty-three isolates were selected and used for further studies. The antagonistic test demonstrated that 5 isolates were able to inhibit the growth of tomato pathogen Xanthomonas campestri pv. vesicatoriar. Inoculation of Bacillus sp. NYPO 7 was successfully used to control plant disease, as can be seen from the decrease of disease index from 4.3 to 2.4. Higher biomass of tomato seedlings were also recorded in Bacillus sp. NYPO 7 or Pantoea sp. MYEO 12 inoculating treatment after 6 weeks of cultivation. Considered for plant growth promoting traits, the highest free-living nitrogen fixing activities ranging from 0.136-0.456 ηmol/ (tube x hr) were obtained. Five isolates showed promising tricalcium phosphate solubilizing activities, and the soluble P ranged from 225 to 1430 μg mL-1 after 4 days of cultivation. All 23 isolates possessed IAA producing abilities. Six isolates were used separately as inoculants in pot experiment to evaluate their performance on tomato growth. The results demonstrated that dry weight of stems and leaves was slightly higher in half dose of chemical fertilization along with Lysinibacillus sp. NYPO 3 inoculating treatment than that in half dose of chemical fertilization treatment only. Distinct community level physiological profiles were obtained after bacterial inoculation, demonstrating that soils under different treatments harbored microorganisms with different carbon catabolic potential.
URI: http://hdl.handle.net/11455/96237
文章公開時間: 10000-01-01
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