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標題: 玉米稈顆粒大小對纖維酵素水解之研究
Study on the Influence of Particle Size on Enzymatic Hydrolysis of Corn Stalk
作者: 林長彥
Chang-Yan Lin
關鍵字: 玉米稈
Corn stover
Enzymatic hydrolysis transformation
Particle size
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摘要: 本研究以玉米為原料,探討利用酵素水解,於不同平均粒徑大小(0.20824mm、0.12364mm、0.08772mm及0.05232mm)與不同固液比(1:8及1:10)PH值在4.3~4.5、溫度50~52℃及連續攪拌等條件下進行酵素水解醣化。研究結果觀察到粒徑大小大於0.149mm只需觀察6小時,粒徑大小小於0.149mm只需要觀察26小時即可得到最終濃度,可有效利用實驗時間。當平均粒徑大小研磨到0.05232mm其醣化轉糖率高達75%及最終糖濃度皆為最佳。表顆粒小可將纖維素結晶破壞較為明顯,使醣化效果較佳,其中平均粒徑大小在0.05232mm時,醣化1小時以內時糖濃度上升更為明顯, 效率為每5分鐘上升糖濃度約0.11%,在5分鐘內平均每30秒上升糖濃度0.12%。 由不同固液比發現1:8最終濃度皆高於1:10,經計算轉糖率清楚了解糖化固液比1:10比1:8更能將纖維素及半纖維轉換成糖,轉糖率並高出1.8% ~3.1%。此值由統計分析為不顯著影響。由上述實驗固液比會增加糖濃度,但糖化效率比例及轉糖率以1:10為較佳。 本研究建立糖濃度在粒徑大小小於0.149mm之方程式與結果有良好的相關性,其決定係數R2為0.93;0.05232mm平均粒徑大小探討完整的醣化過程與方程式,其決定系數R2為0.896。未來可提供相關實驗進行酵素水解研究之評估。
This study examined the efficiency of enzymatic hydrolysis transformation by different particle sizes and different ratio of the corn stover powder to the citric acid liquid (solid-liquid ratio). There are four particle mean sizes: 0.20824mm, 0.12364mm, 0.08772mm, and 0.05232mm; and two solid-liquid ratio: 1:8 and 1:10. The process of enzymatic hydrolysis and saccharification was processed in the condition of ph4.3-4.5, temperature of 50-52℃ and countinuous stirring. The particle mean size of 0.05232mm had the better result.The saccharification transformation is up to 75% and the final concentration is the highest. Moreover, the sugar concentration during saccharification in first hour increased significantly. In the first hour, the study observed the sugar concentration increasing about 0.12% every 30 seconds in the first 5 minutes, and after 5 minutes, it increased 0.11% every 5 minutes untill the first hour. Furthermore, the minimum experimental time is only 6 hours for the particle mean sizes larger than 0.149mm and 26 hours for the sizes smaller than 0.149mm. For the different solid-liquid ratio, the result showed the sugar conversion rate of the solid-liquid ratio 1:10 is higher than the ratio 1:8, although the final sugar concentration of the solid-liquid ratio 1:8 is higher than the ratio 1:10. In other words, the solid-liquid ratio 1:10 transformed cellulose and hemicellulose to sugar more efficiently. Above all, this study has simulated the enzymatic hydrolysis transformation of corn stover for particle mean size 0.149mm and 0.05232mm, and the R2 are 0.93 and 0.896 respectively. Both of the simulated equations are highly correlation. The simulated mathematics model can be applied to describe enzymatic hydrolysis transformation.
文章公開時間: 2020-08-28
Appears in Collections:生物產業機電工程學系



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