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標題: 利用無針式注射裝置進行蛋內接種
Study of In Ovo Vaccination by Needle-free Injection Device
作者: 陳韋志
Wei-Chih Chen
關鍵字: 蛋內注射
Injection in ovo
Needle-free injection
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摘要: 台灣目前針對肉雞的防疫主要以人工為主,相關肉雞雞胚蛋內注射疫苗自動化系統技術仍掌握在國外大廠中,人工方式對雞隻免疫與進口自動化蛋內疫苗注射機台相比,成本較低卻耗時又費人力,雛雞因等待接種疫苗時間過長易造成雞隻間疾病交叉感染,在人工施打疫苗過程中,雛雞亦容易緊迫而發生食欲不振等問題。為因應民生需求、農業技術以及生技醫療產業之重要性,蛋內注射之相關技術有本土化的必要,本研究使用自製無針式注射器對孵化中之蛋胚進行蛋內注射,首先進行機台測試,接著找出最合適的注射力道,最後將準備進行接種的胚雞蛋分為兩組,分別注射傳染性支氣管炎(Infectious bronchitis, IB)疫苗與注射用水於發育中18.5日之SPF雞胚蛋,日齡於21日時雛雞順利出雛,實驗結果顯示,實驗組與對照組孵化率並無太大差異,孵化後連續3週進行抽血檢驗抗體,於第3週時大部分雞隻有產生抗體。因此可以確定在合適的注射壓力下,以無針式注射進行蛋內接種,不會影響孵化率,而且能將疫苗注射送入胚雞體內,使雛雞在出雛後自體產生抗體。
Vaccination for broilers mainly bases on workers in Taiwan. There are automated machines for broilers-vaccination, but the cost is too high. Injecting each new born chicken with vaccines spends too much times, it could lead to cross-infection diseases among chicken. Injecting each new born chicken with vaccines also makes chicken stress so that loss appetite. Differ from other technology, this study focused on in ovo neddle-free injection. First, we carried out the machine test and found the most suitable injection force. Then the eggs of embryos were prepared into two groups . One is injecting in ovo hatching day-18.5 eggs with water, and the other is injecting in ovo with IB MA5. Chicken broke shells at day-21. The results show that the hatching rate did not too much of a difference from two groups. After incubation, blood tests were performed once a week for 3 times. At week 3, most chickens can produce antibodies. Therefore, needle-free injection will not affect the hatching rate and chickens can only produce antibodies themselves.
文章公開時間: 2020-07-20
Appears in Collections:生物產業機電工程學系



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