Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/96459
標題: 利用無針式注射裝置進行蛋內接種
Study of In Ovo Vaccination by Needle-free Injection Device
作者: 陳韋志
Wei-Chih Chen
關鍵字: 蛋內注射
無針式注射器
Injection in ovo
Needle-free injection
引用: (1) 林茂勇。2003。胚胎免疫與蛋內注射疫苗。疫苗發展技術與實驗, 動物基因轉殖與疫苗發展技術教學資源中心,教育部顧問室。 (2) 林子元。2014。無針式蛋內疫苗注射之研究。碩士論文。國立中興大學。 (3) 陳鴻毅、黃久珍、徐慶霖。2014。蛋內疫苗注射之最佳位置之研究。國立中興大學。 (4) 王澤瑋。2009。可攜式肌肉注射裝置之可行性分析與設計。國立交通大學。 (5) Burmester, B. R. and J. M. Sharma. 1984. Disease control in avian species by embryonal vaccination. U.S. Patent 4: 458,630. (6) Chen, D., et al. 2000. Epidermal immunization by a needle-free powder delivery technology: immunogenicity of influenza vaccine and protection in mice. Nature medicine 6.10: 1187-1190. (7) Chen, K., et al. 2009. The evaluation of mechanical performance of a jet injection system. Chinese journal of medical instrumentation 33.5: 339-343. (8) Corley, M. M., et al. 2000. Detection of infectious bursal disease vaccine viruses in lymphoid tissues after in ovo vaccination of specific-pathogen-free embryos. Avian Diseases 45.4: 897-905. (9) Corley, M. M. and J. J. Giambrone. 2002. Immunosuppression in specific-pathogen-free broilers administered infectious bursal disease virus vaccines by in ovo route. Avian Diseases 46.4: 810-815. (10) Cox, H. R. 1938. Use of yolk sac of developing chick embryo as medium for growing rickettsiae of Rocky Mountain spotted fever and typhus groups. Public Health Reports: 2241-2247. (11) Dominy, R. G. 1990. The influence of slipstreaming on the performance of a Grand Prix racing car. Journal of Automobile Engineering 204.1: 35-40. (12) DR, K., et al. 2012. Protection from clinical disease against three highly virulent strains of Newcastle disease virus after in ovo application of an antibody-antigen complex vaccine in maternal antibody-positive chickens. Avian Diseases 56.3: 555-560. (13) Giudice, E. L. and J. D. Campbell 2006. Needle-free vaccine delivery. Advanced drug delivery reviews 58: 69-89. (14) H, O., et al. 2009. Application of aluminum hydroxide for an in ovo live Newcastle disease vaccine. Avian Diseases 53.3: 392-395. (15) Hirst, G. K. 1947. Comparisons of influenza virus strains from three epidemics. The Journal of experimental medicine 86.5: 367-381. (16) Huang, Y. P., et al. 2005. Evaluation of an attenuated TW II infectious bronchitis vaccine. Taiwan Vet J 32(2): 123-128. (17) Ju, B. F., et al. 2002. A novel technique for characterizing elastic properties of thin biological membrane. Mechanics of materials 34.11: 749-754. (18) Kendall, M. A. F. 2010. Needle-free vaccine injection. Drug Delivery. M. Schaぴfer-Korting: 193-219. (19) Konashi, S., et al. 2000. Effect of dietary essential amino acid deficiency on immunological variables in broiler chickens. Br. J. Nutr. 83: 449-456. (20) Koprowski H, C. H. 1948. Studies on chick-embryo-adapted rabies virus; culture characteristics and pathogenicity. Journal of immunology 60: 533-544. (21) Levine., M. M. 2003. Can needle-free administration of vaccines become the norm in global immunization. Nat. Med. 9: 99-103. (22) LN, B., et al. 1956. Vaccination against smallpox I. lyophilized vaccinia virus from infected Chorio-Allantoic membranes. The Journal of Immunology 77.5: 332-339. (23) MARIS, E. P., et al. 1949. Vaccination of children with various chorioallantoic passages of measles virus a follow-up study. Pediatrics 4.1: 1-8. (24) Moran, T. and H. P. Hale. 1936. Physics of the Hen's egg I. membranes in the egg. Journal of Experimental Biology 13.1: 35-40. (25) Negash, T., et al. 2011. Comparison of in ovo and post‐hatch vaccination with particular reference to infectious bursal disease. Veterinary Quarterly 26(2): 76-87. (26) North, M. O. and D. D. Bell 1990. Commercial chicken production manual. An avi book. (27) Oshop, G. L., et al. 2003. In ovo delivery of DNA to the avian embryo. Vaccine 21: 1275-1281. (28) Phillips, J. 2007. In the slipstream. Journal of Intellectual Property Law & Practice 2.12: 781. (29) Reddy, S. K., et al. 1996. Protective efficacy of a recombinant herpesvirus of turkeys as an in ovo vaccine against Newcastle and Marek's diseases in specific-pathogen-free chickens. Vaccine 14(6): 469-477. (30) Schramm, J. and S. Mitragotri. 2002. Transdermal drug delivery by jet injectors: energetics of jet formation and penetration. Pharmaceutical research 19.11: 1673-1679. (31) Sharma, J. M. and B. R. Burmester. 1982. Resistance of Marek's disease at hatching in chickens vaccinated as embryos with the turkey herpesvirus. Avian Diseases: 134-149. (32) Sharma, J. M., et al. 1976. Effect of in vitro adaptation of Marek's disease virus on pock induction on the chorioallantoic membrane of embryonated chicken eggs. Infection and Immunity 13(1): 292-295. (33) Shergold, O. A., et al. 2006. The penetration of a soft solid by a liquid jet, with application to the administration of a needle-free injection. Journal of biomechanics 39.14: 2593-2602. (34) Souza, F. M. d. and C. S. Animale. 2008. Basic aspects of in-ovo injection in commercial hatcheries. Ceva animal health asia. (35) Swain, B. K. and T. S. Johri. 2000. Effect of supplemental methionine, choline and their combination on the performance and immune response of broilers. Br. Poult. Sci 41: 83-88. (36) Villalobos., T. 2012. The optimal time for in ovo vaccination., Embrex. (37) Wakenell, P. S., et al. 2002. Effect of in ovo vaccine delivery route on herpesvirus of turkeys/SB-1 efficacy and viremia. Avian Diseases 46: 274-280. (38) Williams, C. J. 2011. In ovo vaccination and chick quality. International Hatchery Practice 19: 7-13. (39) Williams, C. J. and B. A. Hopkins. 2011. Field evaluation of the accuracy of vaccine deposition by two different commercially available in ovo injection systems. Poultry science 90.1: 223-226. (40) X, Z., et al. 2012. Protection conferred by a recombinant Marek's disease virus that expresses the spike protein from infectious bronchitis virus in specific pathogen-free chicken. Virology journal 9.1: 1-10. (41) Ziegler, A. 2007. Needle-free injection--science fiction or comeback of an almost forgotten drug delivery system. Medizinische Monatsschrift fur Pharmazeuten 30.8: 297-303.
摘要: 台灣目前針對肉雞的防疫主要以人工為主,相關肉雞雞胚蛋內注射疫苗自動化系統技術仍掌握在國外大廠中,人工方式對雞隻免疫與進口自動化蛋內疫苗注射機台相比,成本較低卻耗時又費人力,雛雞因等待接種疫苗時間過長易造成雞隻間疾病交叉感染,在人工施打疫苗過程中,雛雞亦容易緊迫而發生食欲不振等問題。為因應民生需求、農業技術以及生技醫療產業之重要性,蛋內注射之相關技術有本土化的必要,本研究使用自製無針式注射器對孵化中之蛋胚進行蛋內注射,首先進行機台測試,接著找出最合適的注射力道,最後將準備進行接種的胚雞蛋分為兩組,分別注射傳染性支氣管炎(Infectious bronchitis, IB)疫苗與注射用水於發育中18.5日之SPF雞胚蛋,日齡於21日時雛雞順利出雛,實驗結果顯示,實驗組與對照組孵化率並無太大差異,孵化後連續3週進行抽血檢驗抗體,於第3週時大部分雞隻有產生抗體。因此可以確定在合適的注射壓力下,以無針式注射進行蛋內接種,不會影響孵化率,而且能將疫苗注射送入胚雞體內,使雛雞在出雛後自體產生抗體。
Vaccination for broilers mainly bases on workers in Taiwan. There are automated machines for broilers-vaccination, but the cost is too high. Injecting each new born chicken with vaccines spends too much times, it could lead to cross-infection diseases among chicken. Injecting each new born chicken with vaccines also makes chicken stress so that loss appetite. Differ from other technology, this study focused on in ovo neddle-free injection. First, we carried out the machine test and found the most suitable injection force. Then the eggs of embryos were prepared into two groups . One is injecting in ovo hatching day-18.5 eggs with water, and the other is injecting in ovo with IB MA5. Chicken broke shells at day-21. The results show that the hatching rate did not too much of a difference from two groups. After incubation, blood tests were performed once a week for 3 times. At week 3, most chickens can produce antibodies. Therefore, needle-free injection will not affect the hatching rate and chickens can only produce antibodies themselves.
URI: http://hdl.handle.net/11455/96459
文章公開時間: 2020-07-20
Appears in Collections:生物產業機電工程學系

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