Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/96532
標題: 探討添加Lactobacillus formosensis S215T 對半乾盤固草與尼羅草青貯料品質與乳羊泌乳性能之影響
The effect of Lactobacillus formosensis S215T inoculants on the quality of pangolagrass and nilegrass haylage and lactation performance of lactating goats
作者: 楊倩如
Chein-Ru Yang
關鍵字: Lactobacillus formosensis S215T
盤固草
尼羅草
半乾青貯料
Lactobacillus formosensis S215T
Pangolagrass
Nilegrass
Haylage
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摘要: 本試驗為評估具產酸與抑菌能力之同質型發酵乳酸菌 (Lactobacillus formosensis S215T) 接種至國產牧草調製半乾青貯料探討對青貯品質、體外瘤胃發酵特性與乳羊泌乳性能之影響。試驗一進行半乾青貯料品質試驗,以盤固草 (A254,41.49% DM) 與尼羅草 (Acroceras macrum,37.40% DM) 半乾青貯料為處理對象,分別接種109 CFU/kg L. formosensis S215T 菌液 (一公噸牧草添加10 L菌液),製成膠膜捆包 (300 - 400 kg/bale),處理組分別為: 盤固草未接菌 (PS)、盤固草+L. formosensis S215T (PS+L) 與尼羅草未接菌 (NS)、尼羅草+L. formosensis S215T (NS+L)。經青貯五個月後顯示,接菌處理之兩種半乾青貯料pH值、醋酸、丁酸以及梭狀桿菌菌數顯著低於未接菌組,並提升乳酸與Flieg氏評分 (P < 0.05)。PS+L組之酵母菌與大腸桿菌群菌菌數顯著低於PS組 (P < 0.05) ; 有氧穩定性於開封後第二、三與五天進行青貯料溫度、pH值、酵母菌與梭狀桿菌之檢測,結果顯示,於開封後第二天,PS、PS+L與NS、NS+L組之酵母菌菌數皆大於5 Log10 CFU/g FM (8.58 vs.7.72與7.13 vs. 8.36 Log10 CFU/g FM) 以及pH值大於4.40 (5.85 vs. 5.27與5.08 vs. 4.98) 之現象,且NS+L組青貯料核心溫度比NS組提早14小時高於室溫2℃,顯示接菌處理組對於改善青貯料有氧穩定性之效果並不明顯。試驗二進行體外瘤胃消化試驗,將青貯料調製成完全混合日糧並使用兩頭瘤胃開窗荷蘭乾乳牛提供瘤胃微生物,評估試驗飼糧營養價值,結果顯示,體外發酵之瘤胃液揮發性脂肪酸、pH值以及體外真消化率未受接菌處理所影響 (P > 0.05),48小時之累積淨產氣量以NS+L日糧組顯著低於NS日糧組 (161.78 vs. 170.83 mL/g DM) (P < 0.05),顯示接菌處理對於提升飼糧營養價值並不明顯。試驗三進行泌乳羊飼養試驗,試驗選取20頭平均泌乳量2.82 kg/d之阿爾拜因及薩能二品種乳羊,PS、PS+L與NS、NS+L組之飼糧採任食與羊隻個別分欄餵飼,試驗共28天 (14天適應期與14天採樣期),結果顯示,PS+L飼糧組其乳脂校正乳泌乳效率較PS飼糧組高之趨勢 (P < 0.1) 與降低乳蛋白率之現象 (P < 0.05),而乾物質採食量、產乳量、其他乳成分與血液生化值則無顯著差異 (P > 0.05)。綜觀上述試驗結果,可能由於添加L. formosensis S215T未能有效改善半乾青貯料之有氧穩定性,造成開封後半乾青貯料腐敗之問題,導致乳羊泌乳性能無明顯改善之效果,但具有提升盤固草與尼羅草半乾青貯料品質與Flieg氏評分,達到保存牧草營養價值之效果。
The objective of this study was to evaluate the effects of nilegrass and pangolagrass inoculated by Lactobacillus formosensis S215T under field condition on haylage quality, in vitro rumen fernentatuin and milk characteristics of dairy goats. In trial 1, pangolagrass (A254, 41.49% DM) and nilegrass (Acroceras macrum, 37.40% DM) were ensiled with or without inoculation of 109 CFU/kg L. formosensis S215T. All forage packaged in large-round bales (300-400 kg/bale). Treatments were: Pangolagrass haylage (PS) vs. Pangolagrass haylage+L. formosensis S215T (PS+L) and Nilegrass haylage (NS) vs. Nilegrass haylage+L. formosensis S215T (NS+L). After five months of fermentation, both silage inoculated L. formosensis S215T could decreased silage pH, acetic acid, butyric acid content and inhibited the clostridia, and increase lactic acid and Flieg's score compared with not inoculated treatments (P < 0.05). PS+L treatment underwent an inhibited the yeasts and coliform bacteria (P < 0.05) compared with PS treatment. Under aerobic conditions, the silage was sampled after 2, 3 and 5 day of aerobic exposure to detect silage temperature, pH, yeasts and clostridia. After silo opening for 2 day, PS, PS+L and NS, NS+L treatments which concentrations of yeasts higher than 5 Log10 CFU/g (8.58 vs. 7.72 and 7.13 vs. 8.36 Log10 CFU/g) and pH values higher than 4.40 (5.85 vs. 5.27 and 5.08 vs. 4.98), whereas NS+L treatment reduced aerobic stability for 14 h as compared with the NS treatment, the results indicating that inoculated L. formosensis S215T were not improved aerobic stability. As regarding in vitro rumen incubation in trial 2, silage treatments combined with various feeds as a total mixed ration to evaluate the nutritive value of experiment dietary on rumen cannulated cows. The results showed that in vitro rumen fermentation VFA, pH values of rumen fluid and in vitro true digestibility had no significant differences among the groups, but NS+L treatment decreased the 48 h net gas production as compared with the NS treatment (161.78 vs. 170.83 mL/g DM, respectively), the results indicating that inoculated L. formosensis S215T were not improved nutritive value of dietary. In trial 3, a total of 20 head Alpine and Saanen dairy goats were selected which milk production an average of 2.82 kg/d. Goats were raised in individual pens and assigned into PS, PS+L and NS, NS+L groups randomly. The trials with 28 days, the first 14 day were used for adjustment to diets followed by 14 day of sample collection. The results showed that PS+L treatment tended to increase FCM efficiency (P < 0.1) and decreased milk protein content compared with PS treatment (P < 0.05). All groups had no significant effect on daily dry matter intake, milk production, blood parameters and milk compositions except milk protein content (P > 0.05). In conclusion, maybe because of the inoculation of L. formosensis S215T can't improve the aerobic stability of silage, lead to lack of significant of lactating goats on milk performance. However, there is still potential of adding L. formosensis S215T on pangolagrass haylage and nilegrass haylage to improve the fermentation quality and Flieg's score.
URI: http://hdl.handle.net/11455/96532
文章公開時間: 2020-08-22
Appears in Collections:動物科學系

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