Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/97724
標題: 環境鹽度對墨綠凹鼻魨(Dichotomyctere nigroviridis)小腸中寡肽吸收機制相關蛋白表現的影響
Salinity Effects on Expressions of Oligopeptide-Absorbing-Mechanisms Related Proteins in the Intestine of the Spotted Green Puffer (Dichotomyctere nigroviridis)
作者: 鄭元傑
Yuan-Chieh Cheng
關鍵字: 寡肽
環境鹽度
墨綠凹鼻魨
oligopeptide
environmental salinity
spotted green puffer
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摘要: 廣鹽性魚類的腸道同時具有養分吸收和滲透壓調節的功能,藉由鈉鉀幫浦(NKA)或是氫離子幫浦(VHA)所製造的細胞膜內外離子梯度作為動力,調控腸道物質進出,而寡肽在魚類腸道是一種重要的蛋白質養分吸收型態,它的吸收是藉由鈉氫交換蛋白(NHE)和寡肽吸收蛋白(PepT1)共轉運入胞內,為了解腸道細胞膜內外離子梯度面臨鹽度變化時,養分吸收機制的可能改變,本實驗即以探討墨綠凹鼻魨小腸寡肽吸收機制相關蛋白在不同環境鹽度下的變化為目的。結果顯示,在解剖層次,淡水馴養組別的小腸相對長度及微絨毛量都有顯著增加。在分子與蛋白表現層次,淡水組的PepT1, NKA及NHE2表現量亦皆有顯著提升。由本研究的結果推論,不同環境鹽度下調控寡肽吸收機制的離子梯度可能來自位在腸道表皮細胞NKA的參與,而VHA在不同鹽度環境中其活性表現並沒有顯著變化,可能不參與小腸寡肽的吸收。最後,經由小腸的形態特徵與寡肽吸收相關運輸蛋白的表現推論,本研究認為墨綠凹鼻魨在淡水環境中對寡肽吸收有較高的需求。
The intestines of the euryhaline teleosts are capable of both the functions in nutrient absorption and osmoregulation, and the In and out of intestinal substances are regulated by the intracellular and extracellular ion gradients made by Na+-K+ATPase (NKA) or vacuolar-type H+-ATPase (VHA). Oligopeptide is the crucial form of protein absorption in the fish gut, and its absorption is co-transported into the cell by sodium hydrogen exchange protein (NHE) and oligopeptide absorption protein (PepT1). In order to understand the possible changes in the absorption mechanism of nutrients when the intestinal membrane is facing salinity changes. This thesis is aim to investigate the changes of proteins related to the oligopeptides absorption mechanism of small intestine in the spotted green puffer in different environmental salinities. In the anatomical aspects, the relative length and amount of villin of the intestines increased in the FW group. In the molecular and protein aspects, the expression of PepT1, NKA and NHE2 also increased in the FW group. It is inferred from the results that the ion gradients that regulate the absorption mechanism of oligopeptides under different environmental salinities may be derived from the involvement of NKA in the intestinal epithelial cells, while VHA has no significant changes in its activity in different salinity environments and may not participate in the absorption of intestinal oligopeptides. Finally, the upregulation of oligopeptide absorption-related transporters and the plasticity of intestine morphological traits revealed that there is a higher demand of oligopeptide absorption in FW-acclimated spotted green puffer.
URI: http://hdl.handle.net/11455/97724
文章公開時間: 10000-01-01
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