Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/97799
標題: 免標定式電化學阻抗免疫感測技術用於類風溼性關節炎治療藥物之監測平台的研發
Development of Label-Free Electrochemical Impedimetric Immunosensors for The Therapeutic Drug Monitoring of Rheumatoid Arthritis
作者: 蔡沛宇
Pei-Yu Tsai
關鍵字: 治療藥物監測
電化學阻抗頻譜分析法
adalimumab
免疫感測晶片
therapeutic drug monitoring
electrochemical impedance spectrum
adalimumab
immunosensor chips
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摘要: 治療藥物監測(therapeutic drug monitoring, TDM)是一種維持藥物療效且同時降低藥物副作用的臨床監測行為,因此開發一快速且準確的TDM平台具有臨床的重要性。本研究以免標定式電化學阻抗頻譜分析法(electrochemical impedence spectroscopy, EIS)製作一快速檢測adalimumab濃度之免疫感測器,以濺鍍薄膜金電極為基材,探討以Protein A(PA)進行方向性固定anti-adalimumab抗體,評估對adalimumab的感測特性。此外,也以化鎳浸金(electroless nickel immersion gold, ENIG)之印刷電路板(printed circuit board, PCB)電極作為基材,探討此基材製作免疫感測器之特性。結果顯示以濺鍍薄膜金所做之感測器可得到線性範圍為1 ng/mL – 100 ng/mL;而化鎳浸金之PCB電極以單片電極僅量測一次的使用程序上,進行檢測可在0.5 μg /mL – 40 μg /mL取得良好的線性關係。但是兩種電極皆具有再現性不佳之問題。未來透過大量化製作技術的改善,應可提升電極的再現性,利於免標定式TDM檢測電極的商業發展。
Therapeutic drug monitoring (TDM) is a clinical monitoring behavior which requires to maintain drug efficacy and reduce drug side effects. Therefore, it is important to develop a fast and accurate TDM detection for clinical diagnosis. In this research, an immunosensor based on electrochemical impedance spectroscopy (EIS) for the rapid detection of adalimumab concentration was prepared. Gold electrodes fabricated by sputter deposition on a glass substrate were fabricated to explore the directional immobilization of anti-adalimumab antibody on a protein A (PA) -modified layer. Furthermore, copper printed circuit board (PCB) electrodes with the electroless nickel immersion gold (ENIG) deposition were used to develop an adalimumab immunosensor. The results show that the sputtered thin-film gold electrodes had a good linearity in the range of 1 ng / mL - 100 ng / mL. The PCB ENIG electrodes can show a good linearity in the range of 0.5 μg / mL - 40 μg / mL by using one-shot test. However, both electrodes lack good reproducibility. In the future the massive production of electrodes can improve the reproducibility of immunosensors, which is benefit for the commercialization of TDM detector.
URI: http://hdl.handle.net/11455/97799
文章公開時間: 2021-08-03
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