Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/98503
標題: 以果蠅動物模式檢測生物毒性—以DEHP為例
Detection of Biological Toxicity in Drosophila Animal Models:Take DEHP as an Example
作者: 陳梅瑩
Mei-Ying Chen
關鍵字: 毒性
鄰苯二甲酸二(2-乙基己基)酯
壽命
生殖
運動功能
求偶行為
網膜膜電位
電壓鉗
果蠅
DEHP
toxicity
lifespan
reproduction
motor function
ERG
voltage clamp
courtship behavior
Drosophila
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摘要: 現今環境污染的危害日趨嚴重,對動植物、棲息地以及生態嚴造成重破壞,亦對人類產生嚴重的負面影響。而人類製造出來的非天然的化學物質,常因誤認為是安全的,因而無形中成為隠形的殺手。當今全球塑料污染是重要的課題之一,而塑料中的鄰苯二甲酸二(2-乙基己基)酯(Di(2-ethylhexyl)phthalate, DEHP)是用量最大塑化劑之一,對環境和人類的傷害尚未完全瞭解,因此,希望以DEHP為例,透過環境中常見的生物——果蠅,運用及發展更廣泛性的各種生理指標,以評估化學物質的生物毒性。本論文分析8項生物性指標及潛在危害性風險評估:壽命、體重、生殖能力、求偶行為、運動功能、學習與記憶能力、視網膜電位圖及神經肌肉接頭電生理。 結論:果蠅攝食DEHP之不良影響為(1)野生型果蠅壽命減弱 (2)運動功能減弱(3)子代體重改變(4)子代數量減少(5)求偶行為改變(6)視網膜膜電位改變(7)自發及誘發性神經傳遞特性改變(8)學習與記憶能力無顯著差異。體重、求偶行為、視網膜電位圖有性別差異性;其中壽命及求偶行為之DEHP潛在危害性風險,雄性高於雌性;運動功能、體重及生殖能力之DEHP潛在危害性風險,雄性低於雌性;此外,實驗結果也顯示DEHP可以延長阿茲海默症(AD)果蠅的壽命。本論文首次發表,DEHP會影響視網膜和神經肌肉突觸的功能,以及可以延長AD果蠅的壽命。DEHP影響視網膜可能因損害視網膜的感光細胞和層狀神經元之間的突觸信號而影響視網膜功能;DEHP也改變了神經肌肉突觸的自發性和誘發性神經傳遞特性。希望藉此以果蠅動物模式檢測和評估更多的化學物質之未知生物毒性,警示人類避免濫用化學物質造成生物和人類的浩劫。
Nowadays, the harm caused by environmental pollution has become increasingly severe. Environmental pollution causes severe damage to animals, plants, habitats, and ecology. It has also had a serious negative impact on humankind. The non-natural chemical substances produced by humans are often mistaken for safety, and they are virtually a killer. Today, global plastic pollution is one of the most important issues. However, Di(2-ethylhexyl)phthalate(DEHP)is one of the most abundant plasticizers in plastics. The environmental and human injuries caused by DEHP have not yet been fully understood. Therefore, this thesis hopes to use DEHP as an example to apply and develop a wide range of physiological indicators to evaluate the biological toxicity of chemical substances through the common organism, Drosophila, in the environment. In this study, were analyzed eight biological indicators and potentially hazardous of risk assessment: lifespan, weight, reproductive performance, courtship behavior, motor function, learning and memory ability, electroretinogram(ERG) and NMJ electrophysiology. In conclusion, the adverse effects of Drosophila, treated with DEHP, were (1) attenuating survival of wild type flies (2) attenuating motor function (3) altering offspring weight (4) decreasing number of progeny (5) altering courtship behavior (6) altering electroretinogram (ERG) (7) altered both spontaneous and evoked neurotransmission properties of NMJ, but no obviously affecting learning and memory ability. In the three biological indicators, weight, courtship behavior and ERG, there is gender difference. In the two biological indicators, lifespan and courtship behavior, the potentially hazardous risks were higher in male than in female. In the three biological indicators, motor function, weight, reproductive performance, the potentially hazardous risks were higher in male than in female. This was the first study to find that DEHP can affect the function of retina and NMJ synapses, and extend the lifespan of the flies with AD. DEHP exposure may harm vision by affecting the synaptic signaling between the photoreceptor and the laminar neurons. Besides, DEHP can altered both spontaneous and evoked neurotransmission properties of NMJ. In the future, it is hoped to use the Drosophila animal model to detect and estimate the unknown biological toxicity of more chemicals to warn humans that the abuse of the chemicals can cause biological and human catastrophes.
URI: http://hdl.handle.net/11455/98503
文章公開時間: 2022-01-03
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