Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/98523
標題: 乳鐵蛋白、玻尿酸及自體血清眼藥水對兔角膜基質損傷癒合效果之比較
Comparison of lactoferrin, hyaluronic acid, and autologous serum eye drops on corneal stromal wound healing in rabbits
作者: 董盈資
Ying-Tzu Tung
關鍵字: 乳鐵蛋白
玻尿酸
自體血清
角膜潰瘍
lactoferrin
hyaluronic acid
autologous serum
corneal ulceration
引用: Adlerova L, Bartoskova A, and Martin F. 2008. Lactoferrin: A review. Vet Med (Praha) 53(9):457-468. Akyol-Salman I. 2006. Effects of autologous serum eye drops on corneal wound healing after superficial keratectomy in rabbits. Cornea 25(10):1178-1181. Andrew SE, Tou S, and Brooks DE. 2001. Corneoconjunctival transposition for the treatment of feline corneal sequestra: a retrospective study of 17 cases (1990–1998). Vet Ophthalmol 4(2):107-111. Anitua E, Muruzabal F, Tayebba A, Riestra A, Perez VL, Merayo‐Lloves J, and Orive G. 2015. Autologous serum and plasma rich in growth factors in ophthalmology: preclinical and clinical studies. Acta Ophthalmol 93(8):e605-614. Ashby B, Garrett Q, and Willcox M. 2011. Bovine Lactoferrin Structures Promoting Corneal Epithelial Wound Healing In Vitro. Invest Ophthalmol Vis Sci 52(5):2719-2726. Atallah M, and Amescua G. 2014. Advancements in Anterior Lamellar Keratoplasty. In: Jeng BH, editor. Advances in Medical and Surgical Cornea. Berlin, Heidelberg: Springer. p 89-98. Bentley E. 2005. Spontaneous chronic corneal epithelial defects in dogs: a review. J Am Anim Hosp Assoc 41(3):158-165. Boilly B, Vercoutter-Edouart A, Hondermarck H, Nurcombe V, and Le Bourhis X. 2000. FGF signals for cell proliferation and migration through different pathways. Cytokine Growth Factor Rev 11(4):295-302. Bremond-Gignac D, Chiambaretta F, and Milazzo S. 2011. A European perspective on topical ophthalmic antibiotics: Current and evolving options. Ophthalmol Eye Dis 3:29-43. Bukowiecki A, Hos D, Cursiefen C, and Eming SA. 2017. Wound-Healing Studies in Cornea and Skin: Parallels, Differences and Opportunities. Int J Mol Sci 18(6):1257. Chow DW, and Westermeyer HD. 2016. Retrospective evaluation of corneal reconstruction using ACell Vet™ alone in dogs and cats: 82 cases. Vet Ophthalmol 19(5):357-366. DelMonte DW, and Kim T. 2011. Anatomy and physiology of the cornea. J Cataract Refract Surg 37(3):588-598. Di Girolamo N. 2015. Moving epithelia: Tracking the fate of mammalian limbal epithelial stem cells. Prog Retin Eye Res 48:203-225. Dicker KT, Gurski LA, Pradhan-Bhatt S, Witt RL, Farach-Carson MC, and Jia X. 2014. Hyaluronan: a simple polysaccharide with diverse biological functions. Acta biomaterialia 10(4):1558-1570. Dorbandt DM, Moore PA, and Myrna KE. 2015. Outcome of conjunctival flap repair for corneal defects with and without an acellular submucosa implant in 73 canine eyes. Vet Ophthalmol 18(2):116-122. Dua HS, Gomes JA, and Singh A. 1994. Corneal epithelial wound healing. Br J Ophthalmol 78(5):401-408. Dumbleton K, Woods C, and Fonn D. 2009. An investigation of the efficacy of a novel ocular lubricant. Eye & contact lens 35(3):149-155. Eghrari AO, Riazuddin SA, and Gottsch JD. 2015. Overview of the Cornea: Structure, Function, and Development. In: Hejtmancik JF, and Nickerson JM, editors. Prog Mol Biol Transl Sci: Academic Press. p 7-23. Engelmayer J, and Varadhachary A. 2003. Properties and application of recombinant human lactoferrin to enhance healing of diabetic wounds. WOUNDS COMPEND CLIN RES PRACT 15(9):294-301. Eraslan M, and Toker E. 2009. Mechanisms of corneal wound healing and its modulation following refractive surgery. Marmara Med J 22(2):169-178. Farnaud S, and Evans RW. 2003. Lactoferrin—a multifunctional protein with antimicrobial properties. Mol Immunol 40(7):395-405. Featherstone H, and Sansom J. 2000. Intestinal submucosa repair in two cases of feline ulcerative keratitis. Vet Rec 146(5):136-138. Flanagan JL, and Willcox MD. 2009. Role of lactoferrin in the tear film. Biochimie 91(1):35-43. Freegard TJ. 1997. The physical basis of transparency of the normal cornea. Eye 11(4):465. Freire V, Andollo N, Etxebarria J, Hernáez-Moya R, Durán JA, and Morales M-C. 2014. Corneal wound healing promoted by 3 blood derivatives: an in vitro and in vivo comparative study. Cornea 33(6):614-620. Gabison EE, Huet E, Baudouin C, and Menashi S. 2009. Direct epithelial–stromal interaction in corneal wound healing: Role of EMMPRIN/CD147 in MMPs induction and beyond. Prog Retin Eye Res 28(1):19-33. García-Montoya IA, Cendón TS, Arévalo-Gallegos S, and Rascón-Cruz Q. 2012. Lactoferrin a multiple bioactive protein: An overview. Biochim Biophys Acta 1820(3):226-236. Geerling G, Maclennan S, and Hartwig D. 2004. Autologous serum eye drops for ocular surface disorders. Br J Ophthalmol 88(11):1467-1474. Gelatt KN, and Brooks DE. 2011. Surgery of the cornea and sclera. Veterinary Ophthalmic Surgery. Edinburgh: W.B. Saunders. p 191-236. Gelatt KN, Gilger BC, and Kern TJ. 2013. Veterinary ophthalmology. Chichester: John Wiley & Sons. Giannaccare G, Versura P, Buzzi M, Primavera L, Pellegrini M, and Campos EC. 2017. Blood derived eye drops for the treatment of cornea and ocular surface diseases. Transfus Apher Sci 56(4):595-604. Goa KL, and Benfield P. 1994. Hyaluronic Acid: A Review of its Pharmacology and Use as a Surgical Aid in Ophthalmology, and its Therapeutic Potential in Joint Disease and Wound Healing. Drugs 47(3):536-566. Gomes J, Amankwah R, Powell-Richards A, and Dua H. 2004. Sodium hyaluronate (hyaluronic acid) promotes migration of human corneal epithelial cells in vitro. Br J Ophthalmol 88(6):821-825. González-Chávez SA, Arévalo-Gallegos S, and Rascón-Cruz Q. 2009. Lactoferrin: structure, function and applications. Int J Antimicrob Agents 33(4):301. e301-301. e308. Griguer F, Raymond I, and Regnier A. 2001. Preliminary evaluation of the biocompatibility of the small intestinal submucosa (SIS) biomaterial with the rabbit cornea. Rev Med Vet (Toulouse) 152(8/9):597-604. Gronkiewicz KM, Giuliano EA, Sharma A, and Mohan RR. 2017. Effects of topical hyaluronic acid on corneal wound healing in dogs: a pilot study. Vet Ophthalmol 20(2):123-130. Guideline T, and Guideline O. 2001. OECD Guidelines for the Testing of Chemicals. Oecd/Ocde. Guidera AC, Luchs JI, and Udell IJ. 2001. Keratitis, ulceration, and perforation associated with topical nonsteroidal anti-inflammatory drugs. Ophthalmology 108(5):936-944. Hansen PA, and Guandalini A. 1999. A retrospective study of 30 cases of frozen lamellar corneal graft in dogs and cats. Vet Ophthalmol 2(4):233-241. Hartley C. 2010. Treatment of corneal ulcers: What are the medical options? J Feline Med Surg 12(5):384-397. Hartley C. 2014. The lacrimal system. In: Gould D, and McLellan GJ, editors. BSAVA manual of canine and feline ophthalmology. 3rd ed. Gloucester, UK: British Small Animal Veterinary Association. p 167-181. Hayashi S, Osawa T, and Tohyama K. 2002. Comparative observations on corneas, with special reference to Bowman's layer and Descemet's membrane in mammals and amphibians. J Morphol 254(3):247-258. Hirai S-I, Kawahara M, Sakamoto K, Kimura A, and Nakamura M. 2005. Effects of various lubricants on corneal surface regularity in rabbits. J Ocul Pharmacol Ther 21(5):376-381. Ho WT, Chiang TH, Chang SW, Chen YH, Hu FR, and Wang IJ. 2013. Enhanced corneal wound healing with hyaluronic acid and high-potassium artificial tears. Clin Exp Optom 96(6):536-541. Imanishi J, Kamiyama K, Iguchi I, Kita M, Sotozono C, and Kinoshita S. 2000. Growth factors: importance in wound healing and maintenance of transparency of the cornea. Prog Retin Eye Res 19(1):113-129. Johnson ME, Murphy PJ, and Boulton M. 2006. Effectiveness of sodium hyaluronate eyedrops in the treatment of dry eye. Graefes Arch Clin Exp Ophthalmol 244(1):109-112. Kern TJ. 1990. Ulcerative keratitis. Vet Clin North Am Small Anim Pract 20(3):643-666. Khodadoust AA, Silverstein AM, Kenyon KR, and Dowling JE. 1968. Adhesion of regenerating corneal epithelium: the role of basement membrane. Am J Ophthalmol 65(3):339-348. Kieckens E, Rybarczyk J, Cox E, and Vanrompay D. 2018. Antibacterial and immunomodulatory activities of bovine lactoferrin against Escherichia coli O157: H7 infections in cattle. Biometals 31(3):321-330. Kim JY, Choi YM, Jeong SW, and Williams DL. 2009a. Effect of bovine freeze‐dried amniotic membrane (Amnisite‐BA™) on uncomplicated canine corneal erosion. Vet Ophthalmol 12(1):36-42. Kim JY, Won H-J, and Jeong S. 2009b. A retrospective study of ulcerative keratitis in 32 dogs. Int J Appl Res Vet Med 7(1/2):27-31. Kim SJ, Flach AJ, and Jampol LM. 2010. Nonsteroidal anti-inflammatory drugs in ophthalmology. Surv Ophthalmol 55(2):108-133. Kivelä T, Messmer EM, and Rymgayłło-Jankowska B. 2015. Cornea. In: Heegaard S, and Grossniklaus H, editors. Eye Pathology: An Illustrated Guide. Berlin, Heidelberg: Springer Berlin Heidelberg. p 79-154. Lekhanont K, Jongkhajornpong P, Choubtum L, and Chuckpaiwong V. 2013. Topical 100% serum eye drops for treating corneal epithelial defect after ocular surgery. BioMed research international 2013:521315. Li DQ, and Tseng SC. 1997. Differential regulation of keratinocyte growth factor and hepatocyte growth factor/scatter factor by different cytokines in human corneal and limbal fibroblasts. J Cell Physiol 172(3):361-372. Liu CY, and Kao WWY. 2015. Corneal epithelial wound healing. In: Hejtmancik JF, and Nickerson JM, editors. Prog Mol Biol Transl Sci: Academic Press. p 61-71. Liu L, Hartwig D, Harloff S, Herminghaus P, Wedel T, and Geerling G. 2005. An optimised protocol for the production of autologous serum eyedrops. Graefes Arch Clin Exp Ophthalmol 243(7):706-714. Liu Z, Huang AJ, and Pflugfelder SC. 1999. Evaluation of corneal thickness and topography in normal eyes using the Orbscan corneal topography system. Br J Ophthalmol 83(7):774-778. Ljubimov AV, and Saghizadeh M. 2015. Progress in corneal wound healing. Prog Retin Eye Res 49:17-45. Luzi C, Brisdelli F, Iorio R, Bozzi A, Carnicelli V, Di Giulio A, and Lizzi AR. 2017. Apoptotic effects of bovine apo‐lactoferrin on HeLa tumor cells. Cell Biochem Funct 35(1):33-41. Maggs DJ, Miller P, and Ofri R. 2012. Slatter's fundamentals of veterinary ophthalmology: Elsevier Health Sciences. Martin de Bustamante MG, Good KL, Leonard BC, Hollingsworth SR, Edwards SG, Knickelbein KE, Cooper AE, Thomasy SM, and Maggs DJ. 2018. Medical management of deep ulcerative keratitis in cats: 13 cases. J Feline Med Surg:1098612X18770514. Meek KM, and Boote C. 2004. The organization of collagen in the corneal stroma. Exp Eye Res 78(3):503-512. Meek KM, and Knupp C. 2015. Corneal structure and transparency. Prog Retin Eye Res 49:1-16. Merindano MD, Costa J, Canals M, Potau J, and Ruano D. 2003. A comparative study of Bowman's layer in some mammals: relationships with other constituent corneal structures. European Journal of anatomy 6(3):133-140. Mitchell N. 2014. The sclera, episclera and limbus. In: Gould D, and McLellan GJ, editors. BSAVA manual of canine and feline ophthalmology. 3rd ed. Gloucester, UK: British Small Animal Veterinary Association. p 232-240. Moreno-Expósito L, Illescas-Montes R, Melguizo-Rodríguez L, Ruiz C, Ramos-Torrecillas J, and de Luna-Bertos E. 2018. Multifunctional capacity and therapeutic potential of lactoferrin. Life Sci 195:61-64. Munger RJ. 2002. Veterinary ophthalmology in laboratory animal studies. Vet Ophthalmol 5(3):167-175. Murphy CJ, Marfurt CF, McDermott A, Bentley E, Abrams GA, Reid TW, and Campbell S. 2001. Spontaneous chronic corneal epithelial defects (SCCED) in dogs: clinical features, innervation, and effect of topical SP, with or without IGF-1. Invest Ophthalmol Vis Sci 42(10):2252-2261. Nakamura S, Okada S, Umeda Y, and Saito F. 2004. Development of a rabbit model of tear film instability and evaluation of viscosity of artificial tear preparations. Cornea 23(4):390-397. Netto MV, Mohan RR, Ambrósio Jr R, Hutcheon AE, Zieske JD, and Wilson SE. 2005. Wound healing in the cornea: a review of refractive surgery complications and new prospects for therapy. Cornea 24(5):509-522. Ollivier F, Gilger B, Barrie K, Kallberg M, Plummer C, O'Reilly S, Gelatt K, and Brooks D. 2007. Proteinases of the cornea and preocular tear film. Vet Ophthalmol 10(4):199-206. Ollivier FJ. 2003. Bacterial corneal diseases in dogs and cats. Top Companion Anim Med 18(3):193-198. Öztafl YE, and Özgünefl N. 2005. Lactoferrin: a multifunctional protein. Adv Mol Med 1(4):149-154. Parry N, and Maggio F. 2007. Uveitis in cats. UK Vet Companion Animal 12(3):70-78. Pattamatta U, Willcox M, Stapleton F, Cole N, and Garrett Q. 2009. Bovine lactoferrin stimulates human corneal epithelial alkali wound healing in vitro. Invest Ophthalmol Vis Sci 50(4):1636-1643. Pattamatta U, Willcox M, Stapleton F, and Garrett Q. 2013. Bovine lactoferrin promotes corneal wound healing and suppresses IL-1 expression in alkali wounded mouse cornea. Curr Eye Res 38(11):1110-1117. Rah MJ. 2011. A review of hyaluronan and its ophthalmic applications. Optometry 82(1):38-43. Reinach P, and Pokorny K. 2008. The corneal epithelium: clinical relevance of cytokine-mediated responses to maintenance of corneal health. Arq Bras Oftalmol 71(6):80-86. Robert Z, Rafael J, Michael J, Erwin S, Andreas S, Jörg W, and Reinhold E. 2001. Different preparation methods to obtain platelet components as a source of growth factors for local application. Transfusion (Paris) 41(10):1217-1224. Sanchez RF. 2014. The cornea. In: Gould D, and McLellan GJ, editors. BSAVA manual of canine and feline ophthalmology. 3rd ed. Gloucester, UK: British Small Animal Veterinary Association. p 200-231. Schoster J, Wickman L, and Stuhr C. 1995. The use of ultrasonic pachymetry and computer enhancement to illustrate the collective corneal thickness profile of 25 cats. Veterinary and comparative ophthalmology. Semeraro F, Forbice E, Braga O, Bova A, Di Salvatore A, and Azzolini C. 2014. Evaluation of the efficacy of 50% autologous serum eye drops in different ocular surface pathologies. Biomed Res Int 2014:826970. Soontornvipart K, Tuntivanich N, Kecova H, and Raušer P. 2003. Conjunctival pedicle graft in dogs and cats: a retrospective study of 88 cases. Acta Veterinaria Brno 72(1):63-69. Sridhar MS. 2018. Anatomy of cornea and ocular surface. Indian J Ophthalmol 66(2):190. Startup F. 1984. Corneal ulceration in the dog. J Small Anim Pract 25(12):737-752. Stramer BM, Zieske JD, Jung J-C, Austin JS, and Fini ME. 2003. Molecular mechanisms controlling the fibrotic repair phenotype in cornea: implications for surgical outcomes. Invest Ophthalmol Vis Sci 44(10):4237-4246. Torricelli AA, and Wilson SE. 2014. Cellular and extracellular matrix modulation of corneal stromal opacity. Exp Eye Res 129:151-160. Tsubota K, Goto E, Fujita H, Ono M, Inoue H, Saito I, and Shimmura S. 1999. Treatment of dry eye by autologous serum application in Sjögren's syndrome. Br J Ophthalmol 83(4):390-395. Vanore M, Chahory S, Payen G, and Clerc B. 2007. Surgical repair of deep melting ulcers with porcine small intestinal submucosa (SIS) graft in dogs and cats. Vet Ophthalmol 10(2):93-99. Wakabayashi H, Oda H, Yamauchi K, and Abe F. 2014. Lactoferrin for prevention of common viral infections. J Infect Chemother 20(11):666-671. Wang SB, Hu KM, Seamon KJ, Mani V, Chen Y, and Gronert K. 2012. Estrogen negatively regulates epithelial wound healing and protective lipid mediator circuits in the cornea. The FASEB Journal 26(4):1506-1516. Wang X, and Wu Q. 2013. Normal corneal thickness measurements in pigmented rabbits using spectral‐domain anterior segment optical coherence tomography. Vet Ophthalmol 16(2):130-134. West-Mays JA, and Dwivedi DJ. 2006. The keratocyte: corneal stromal cell with variable repair phenotypes. Int J Biochem Cell Biol 38(10):1625-1631. Williams DL, and Mann BK. 2013. A Crosslinked HA-Based Hydrogel Ameliorates Dry Eye Symptoms in Dogs. Int J Biomater 2013:8. Williams DL, Wirostko BM, Gum G, and Mann BK. 2017. Topical Cross-Linked HA-Based Hydrogel Accelerates Closure of Corneal Epithelial Defects and Repair of Stromal Ulceration in Companion Animals. Invest Ophthalmol Vis Sci 58(11):4616-4622. Wilson SA, and Last A. 2004. Management of corneal abrasions. Am Fam Physician 70:123-132. Wilson SE, Mohan RR, Mohan RR, Ambrosio Jr R, Hong J, and Lee J. 2001. The corneal wound healing response: cytokine-mediated Interaction of the epithelium, stroma, and inflammatory cells. Prog Retin Eye Res 20(5):625-637. Wong AC, Wong C, Yuen NS, and Hui S. 2002. Correlational study of central corneal thickness measurements on Hong Kong Chinese using optical coherence tomography, Orbscan and ultrasound pachymetry. Eye 16(6):715. Yamada C, King KE, and Ness PM. 2008. Autologous serum eyedrops: literature review and implications for transfusion medicine specialists. Transfusion (Paris) 48(6):1245-1255. Yanai R, Yamada N, Inui M, and Nishida T. 2006. Correlation of proliferative and anti-apoptotic effects of HGF, insulin, IGF-1, IGF-2, and EGF in SV40-transformed human corneal epithelial cells. Exp Eye Res 83(1):76-83. Yang G, Espandar L, Mamalis N, and Prestwich GD. 2010. A cross-linked hyaluronan gel accelerates healing of corneal epithelial abrasion and alkali burn injuries in rabbits. Vet Ophthalmol 13(3):144-150. Yu F-SX, Yin J, Xu K, and Huang J. 2010. Growth factors and corneal epithelial wound healing. Brain Res Bull 81(2):229-235. Ziakas NG, Boboridis KG, Terzidou C, Naoumidi TL, Mikropoulos D, Georgiadou EN, and Georgiadis NS. 2010. Long‐term follow up of autologous serum treatment for recurrent corneal erosions. Clin Experiment Ophthalmol 38(7):683-687.
摘要: 角膜潰瘍為犬貓常見疾病,是一種有影響視覺、需要緊急處理的疾病。部分角膜潰瘍可以內科療法控制,然而部分病例會建議以外科手術治療,如結膜皮瓣與填補生物醫學材料。然而外科治療在臨床上會有一些使用限制,可能因為病患麻醉風險較高及費用相對昂貴等因素常不被飼主接受。目前多篇研究顯示在角膜淺層潰瘍使用乳鐵蛋白、玻尿酸或者是自體血清眼藥水能夠幫助角膜上皮癒合,然而對角膜基質的作用仍舊未知。本研究的目的為使用乳鐵蛋白、玻尿酸及自體血清眼藥水對兔子角膜基質潰瘍進行治療,比較對角膜損傷的治療效果。 實驗對象為2-3公斤雄性紐西蘭白兔,首先會將35隻兔子依照眼藥水隨機均分成5組,分別為乳鐵蛋白組、乳鐵蛋白陰性對照組、玻尿酸組、自體血清組及磷酸鹽緩衝溶液組,也就是空白對照組。實驗兔會先依照經濟合作暨發展組織的規範進行急性眼部刺激試驗,無異常再開始試驗。在單側角膜中央製造直徑8 mm、深度為375µm的傷口後,依照組別給予眼藥水,每天4次、每次一滴,持續4周。於誘導潰瘍後1、2、3、4、5、6、7、10、13、16、19、22、25、28天進行眼部臨床症狀及角膜潰瘍範圍的觀察,試驗終止時間為誘導潰瘍後第28天,實驗兔安樂死後取下眼球製作切片進行組織病理學檢查,測量角膜中央上皮與基質厚度、依照基質膠原排列與炎症細胞浸潤程度進行分級。結果,於急性眼部刺激試驗中,在給予眼藥水後1、24、48、72小時各組皆無發現角膜不透明度、虹彩病變、結膜充血及水腫。在誘導基質潰瘍後臨床症狀與潰瘍面積於各檢查時間點各組間無統計學上的顯著差異,組織學相關數值在各組間無統計學上的顯著差異。以整個試驗期間為基準,角膜潰瘍的有無與使用玻尿酸還是空白對照組有顯著相關,另外眼前房閃光的有無與使用玻尿酸還是空白對照組同樣有顯著相關,且在自體血清組還是空白對照組之間亦有相關。綜合以上之結果,乳鐵蛋白、玻尿酸及自體血清眼藥水對於改善眼表臨床症狀、加速角膜上皮癒合及幫助角膜基質膠原纖維重建這三方面無顯著之療效。玻尿酸眼藥水能夠避免嚴重前葡萄膜炎的發生以及讓角膜維持在完整非潰瘍的狀態。自體血清同樣能夠避免嚴重前葡萄膜炎的發生。
Serious corneal ulceration is a potentially vision-threatening condition in small animals. Although serious corneal ulceration may respond to conventional medical managements, a number of patients still require surgical interventions, such as conjunctival graft and biomaterial grafts. However, the surgery may be unavailable in some patients owing to high costs or unsuitable body conditions. Several studies have explored the potential of lactoferrin, hyaluronic acid, and autologous serum eye drops to promote wound healing. The aim of this study was to evaluate and compare the efficacy of the three kinds of eye drops for the treatment of experimentally induced corneal stromal wound in rabbits. A total of 35 New Zealand white rabbits were used as experimental animals and divided into five equal groups corresponded to five different eye drops, including lactoferrin (LF), lactoferrin negative control (LF-N), hyaluronic acid (HA), autologous serum (AS), and phosphate buffered saline (blank control, BC). Initially, three rabbits from each group underwent the acute eye irritation test, and no irritation was found. Next, a unilateral 8-mm diameter and 375-µm depth corneal stromal wound was made in each rabbit, and the eye drops of each group was applied topically four times per day for 28 days. Clinical signs and corneal defect area were recorded at 1, 2, 3, 4, 5, 6, 7, 10, 13, 16, 19, 22, 25, 28 days after wounding, and the corneas were excised for histological examination in the end of the experiment. The result showed no significant difference in the clinical ophthalmic examination scores, the corneal epithelial defect area, and the histologic results among the groups at each point of time. From the perspective of the whole experimental period, there was a significant difference in the absence of corneal ulcer between HA and BC group. Besides, there was also a significant difference in the absence of aqueous flare between HA and BC group, and so was AS and BC group. In summary, the application of lactoferrin, hyaluronic acid, and autologous serum eye drops did not accelerate corneal wound healing and improved the clinical signs, but hyaluronic acid is advantageous to maintain the corneal integrity and avoid anterior uveitis compared to phosphate buffered saline. The use of autologous serum eye drops is also advantageous to avoid anterior uveitis compared to phosphate buffered saline.
URI: http://hdl.handle.net/11455/98523
文章公開時間: 2019-08-20
Appears in Collections:獸醫學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.