Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/99271
標題: Mannosyl electrochemical impedance cytosensor for label-free MDA-MB-231 cancer cell detection
作者: Tang, Yi-Hsuan
Lin, Han-Chen
Lai, Chiao-Ling
Chen, Po-Yu
Lai, Chian-Hui
關鍵字: Cancer cell detection
Carbohydrate-cytosensor
Con A
Label-free
MDA-MB-231 cell
Mannose receptor
Benzimidazoles
Breast Neoplasms
Carbon
Cell Line, Tumor
Concanavalin A
Electrodes
Female
HEK293 Cells
Humans
Lectins, C-Type
Limit of Detection
Mannose
Mannose-Binding Lectins
Oxidation-Reduction
Polyethylene Glycols
Receptors, Cell Surface
Biosensing Techniques
Electric Impedance
摘要: A label-free and ultrasensitive electrochemical impedance cytosensor was developed to specifically detect the breast cancer cells MDA-MB-231 via the interaction between the mannosyl glassy carbon electrode (GCE) and the overexpressed mannose receptors on the target cell surface. The mannosyl GCE was prepared through electrografting of the amino-functionalized mannose derivatives on GCE surface in which a covalent bond was formed between carbon of the electrode and the amino group of the mannose derivative. The fluorescent microscopy indicated that the electrode is specific for MDA-MB-231 cells, with good biocompatibility for viable captured cells. The derivative with a shorter alkyl linker, mannose-C2NH2, showed a better sensitivity than that with a longer linker, mannose-C6NH2. GCE modified with amino-functionalized galactose derivative, galactose-C2NH2, shows no function to the detection of MDA-MB-231 cells. The specific interaction between the mannosyl GCE and Con A (a mannose-binding lectin) or MDA-MB-231 breast cancer cells with overexpressed mannose receptors was determined through the change of peak separation in the cyclic voltammogram or the change of charge transfer resistance in the electrochemical impedance spectra (Nyquist plot) in the electrolytes containing a reversible redox couple [Fe(CN)6]3-/[Fe(CN)6]4-. The charge transfer resistance in the Nyquist plots linearly depended on the concentration of MDA-MB-231 cells (1.0 × 10-1.0 × 105 cells mL-1, with 10 cells mL-1 being the lower detection limit). Introducing 0.1% polyethylene glycol-200 (PEG-200) was able to prevent the interference caused by 1.0 × 103 HEK-293T cells mL-1, a non-cancer cell line (control).
URI: http://hdl.handle.net/11455/99271
Appears in Collections:生醫工程研究所

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