Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/99278
標題: Silencing Stem Cell Factor Gene in Fibroblasts to Regulate Paracrine Factor Productions and Enhance c-Kit Expression in Melanocytes on Melanogenesis
作者: Li, Pin-Hui
Liu, Li-Heng
Chang, Cheng-Chung
Gao, Rong
Leung, Chung-Hang
Ma, Dik-Lung
David Wang, Hui-Min
王惠民
關鍵字: fibroblasts
melanin
melanocytes
paracrine factors
stem cell factor
Cell Proliferation
Fibroblasts
Gene Knockdown Techniques
Humans
Melanins
Melanocytes
Proto-Oncogene Proteins c-kit
RNA Interference
Stem Cell Factor
Ultraviolet Rays
Gene Silencing
Paracrine Communication
摘要: Melanogenesis is a complex physiological mechanism involving various paracrine factors. Skin cells such as keratinocytes, fibroblasts, and melanocytes communicate with one another through secreted regulators, thereby regulating the melanocytes' bio-functions. The stem cell factor (SCF) is a paracrine factor produced by fibroblasts, and its receptor, c-kit, is expressed on melanocytes. Binding of SCF to c-kit activates autophosphorylation and tyrosine kinase to switch on its signal transmission. SCF inhibition does not suppress fibroblast proliferation in MTT assay, and SCF silencing induced mRNA expressions of paracrine factor genes, HGF, NRG-1, and CRH in qPCR results. Following UVB stimulation, gene expressions of HGF, NRG, and CRH were higher than homeostasis; in particular, HGF exhibited the highest correlation with SCF variations. We detected fibroblasts regulated SCF in an autocrine-dependent manner, and the conditioned medium obtained from fibroblast culture was applied to treat melanocytes. Melanogenesis-related genes, tyrosinase and pmel17, were upregulated under conditioned mediums with SCF silencing and exposed to UVB treatments. Melanin quantities in the melanocytes had clearly increased in the pigment content assay. In conclusion, SCF silencing causes variations in both fibroblast paracrine factors and melanocyte melanogenesis, and the differences in gene expressions were observed following UVB exposure.
URI: http://hdl.handle.net/11455/99278
Appears in Collections:生醫工程研究所

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