Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/99409
標題: 空氣釋放甲基茉莉花酸對於番茄抗蟲機制之影響
Effect of air release methyl jasmonate on tomato in insects'' resistance
作者: 陳冠勳
Kuan-Hsun Chen
關鍵字: 植物防禦反應
甲基茉莉花酸
多酚氧化酵素
胰蛋白酶抑制劑
斜紋夜蛾
相對生長速率
Plant defense response
methyl jasmonate
polyphenol oxidase
trypsin inhibitor
Spodoptera litura
relative growth rate
引用: 高穗生。1995。昆蟲之大量飼育。農業藥物毒物試驗所專題報導37。1-8。 陳思恩。2015。不同寄主植物對於斜紋夜蛾及馬尼拉小繭蜂生長表現和偏好之影響。中興大學昆蟲系碩士論文。55頁。 彭冠華。2014。甲基茉莉花酸處理番茄種子對幼苗及斜紋夜蛾之影響。中興大學昆蟲系碩士論文。52頁。 費雯綺、王喻其。2007。植物保護手冊─蔬菜篇。229。 臺灣省農業試驗所。1987。台灣地區現有作物栽培品種名錄<茄科篇>。臺灣省農業試驗所。153頁。 羅儒哲。2009。甲基茉莉花酸對不同作物誘導之防禦反應及其對斜紋夜蛾生長表現與產卵偏好之影響。55頁。 Arimura, G., Kost, C., Boland, W. 2005. Herbivore-induced, indirect plant defences. BBA, 1734, 91-111. Belhadj, A., Saigne, C., Telef, N., Cluzet, S., Bouscaut, J., Corio-Costet, M. F., Mérillon, J. M. 2006. Methyl jasmonate induces defense responses in grapevine and triggers protection against erysiphe necator. J. Agric. Food Chem., 54, 9119-9125. Bhonwong, A., Stout, M. J., Attajarusit, J., Tantasawat, P. 2009. Defensive role of tomato polyphenol oxidases against cotton bollworm (Helicoverpa armigera) and beet armyworm (Spodoptera exigua). J. Chem. Ecol., 35, 28-38. Bolter, C. J. 1993. Methyl jasmonate induces papain inhibitor(s) in tomato leaves. Plant Physiol., 103(4), 1347-1353. Bradley, D. J., Kjellbom, P., Lamb, C. J. 1992. Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell, 70, 21-30. Cao, S. F., Zheng, Y. H., Yang, Z. F., Tang, S. S., Jin, P., Wang, K. T., Wang , X. M. 2008. Effect of methyl jasmonate on the inhibition of colletotrichum acutatum infection in loquat fruit and the possible mechanisms. Postharvest Biol. Technol., 49, 301-307. Chen, H., Wilkerson, C. G., Kuchar, J. A., Phinney, B. S., Howe, G. A. 2005. Jasmonate-inducible plant enzymes degrade essential amino acids in the herbivore midgut. Proc. Natl. Acad. Sci., 102(52), 19237-19242. Constabel, C. P., Ryan, C. A. 1994. A survey of wound- and methyl jasmonate-induced leaf polyphenol oxidase in crop plants. Phytochem., 47(4), 507-511. Constabel, C. P., Yip, L., Ryan, C. A. 1998. Prosystemin from potato, black nightshade, and bell pepper: primary structure and biological activity of predicted systemin polypeptides. Plant Mol. Biol., 36, 55-62. Ding, C. K., Wang, C. Y., Gross, K. C., Smith, D. L. 2002. Jasmonate and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to chilling injury in tomato fruit. Planta, 214, 895-901. Farmer, E. E., Ryan, C. A. 1990. Interplant communication: Airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc. Natl. Acad. Sci., 87, 7713-7716. Farmer, E. E., Johnson, R. R., Ryan, C. A. 1991. Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic acid. Plant Physiol., 98, 995-1002. Felton, G. W. 2005. Indigestion is a plant’s best defense. Proc. Natl. Acad. Sci., 102(52), 18771-18772. Fortunato, F. da S., Oliveira, M. G. de A., Brumano, M. H. N., Silva, C. H. O., Guedes, R. N. C., Moreira, M. A. 2007. Lipoxygenase-induced defense of soybean varieties to the attack of the velvetbean caterpillar (Anticarsia gemmatalis Hübner). J. Pest Sci., 80, 241-247. Gadino, A. N., Walton, V. M., Lee, J. C. 2012. Evaluation of methyl salicylate lures on populations of Typhlodromus pyri (Acari: Phytoseiidae) and other natural enemies in western Oregon vineyards. Biological Control, 63, 48-55. Heil, M. 2008. Indirect defence via tritrophic interactions. New Phytologist, 178, 41-61. Jang, G., Shim, J. S., Jung, C. K., Song, J. T., Lee, H. Y., Chung P. J., Kim, J. K., Choi, Y. D. 2014. Volatile methyl jasmonate is a transmissible form of jasmonate and its biosynthesis is involved in systemic jasmonate response in wounding. Plant Biotechnol. Rep., 8, 409-419. Jiang, D., Yan, S. 2018. MeJA is more effective than JA in inducing defense responses. Arthropod-Plant Interactions, 12(1), 49-56. Kant, M. R., Ament, K., Sabelis, M. W., Haring, M. A., Schuurink, R. C. 2004. Differential timing of spider mite-induced direct and indirect defenses in tomato plants. Plant Physiology, 135, 483-495. Karban, R., Agrawal, A. A., Thaler, J. S., Adler, Lynn S. 1999. Induced plant responses and information content about risk of herbivory. Tree, 14(11), 443-447. Koiwa, H., Bressan, R. A., Hasegawa, P. M. 1997. Regulation of protease inhibitors and plant defense. Trend in plant science, 2(10), 379-384. Krisa, S., Larronde, F., Budzinski, H., Decendit, A., Deffieux, G., Mérillon, J. M. 1999. Stilbene production by Vitis vinifera cell suspension cultures: methyl jasmonate induction and 13C biolabeling. J. Nat. Prod., 62, 1688-1690. Ku, K. M., Juvik, J. A. 2013. Environmental stress and methyl jasmonate-mediated changes in flavonoid concentrations and antioxidant activity in broccoli florets and kale leaf tissues. Hort. Sience, 48(8), 996-1002. Larronde, F., Gaudillère, J. P., Krisa, S., Decendit, A., Deffieux, G., Mérillon, J. M. 2003. Airborne methyl jasmonate induces stilbene accumulation in leaves and berries of grapevine plants. Am. J. Enol. Vitic., 1(54), 63-66. Li, C., Wang, P., Menzies, N. W., Lombi, E., Kopittke, P. M. 2018. Effects of methyl jasmonate on plant growth and leaf properties. J. Plant Nutr. Soil Sci., 181, 409-418 Omer, A. D., Granett, J., Karban, R., Villa, E. M. 2001. Chemically-induced resistance against multiple pests in cotton. International J. Pest Manage., 47(1), 49-54. Papadopoulou, G. V., van Dam, N. M. 2017. Mechanisms and ecological implications of plant-mediated interactions between belowground and aboveground insect herbivores. Ecol. Res., 32, 13-26. Prestona, C. A., Lauea, G., Baldwina, I. T. 2001. Methyl jasmonate is blowing in the wind, but can it act as a plant–plant airborne signal? Biochem. System. and Ecol., 29, 1007-1023. Rodriguez-Saona, C., Kaplan, I., Braasch, J., Chinnasamy, D., Williams, L. 2011. Field responses of predaceous arthropods to methyl salicylate: A meta-analysis and case study in cranberries. Biological Control, 59, 294-303. Senaratna, T., Touchell, D., Bunn, E., Dixon, K. 2000. Acetyl salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regul., 30, 157-161. Stout, M. J., Workman, J., Duffey, S. S. 1994. Differential induction of tomato foliar proteins by arthropod herbivores. J. Chem. Ecol., 20(10), 2575-2594. Tan, C. W., Chiang, S. Y., Ravuiwasa, K. T., Yadav, J., Hwang, S. Y. 2012. Jasmonate-induced defenses in tomato against Helicoverpa armigera depend in part on nutrient availability, but artificial induction via methyl jasmonate does not. Arthropod-Plant Interactions, 6, 531-541. Tan, C. W., Lo, J. C., Yadav, J., Ravuiwasa, K. T., Hwang, S. Y. 2011. Methyl jasmonate induced responses in four plant species and its effect on Spodoptera litura Fab. performance. J. Asia Pacific Entomol., 14, 263-269. Thaler, J. S., Farag, M. A., Paré, P. W., Dicke, M. 2002. Jasmonate-deficient plants have reduced direct and indirect defences against herbivores. Ecology Letters, 5, 764–774. Thaler, J. S. 1999. Induced resistance in agricultural crops: effects of jasmonic acid on herbivory and yield in tomato plants. Environ. Entomol., 28(1), 30-37. van Dam, N., Witjes, M. L., Svatoš, A. 2004. Interactions between aboveground and belowground induction of glucosinolates in two wild Brassica species. New Phytol., 161, 801-810. Woods, J. L., James, D. G., Lee, J. C., Gent, D. H. 2011. Evaluation of airborne methyl salicylate for improved conservation biological control of two-spotted spider mite and hop aphid in Oregon hop yards. Exp. Appl. Acarol., 55, 401-416.
摘要: 當昆蟲攻擊植物時,植物體內會累積大量甲基茉莉花酸(methyl jasmonate, MeJA),進而誘導植物的防禦反應,在植物體內產生大量的抗蟲蛋白,降低昆蟲的生長表現。而前人研究發現,對植物噴灑MeJA會直接誘導植物產生抗蟲反應,處理後的葉片餵食斜紋夜蛾幼蟲(Spodoptera litura)也可以降低其生長速率。因為目前農藥管理法的限制,直接噴灑化學物質為農藥的範疇,而過去又鮮少對於空氣釋放誘導植物產生抗性的研究。所以本實驗研究的目的為瞭解番茄在空氣釋放MeJA處理(M)和噴灑MeJA(S)處理之下,植物抗蟲防禦蛋白的改變;另外也探討昆蟲幼蟲餵食不同處理番茄葉片之生長表現之影響。結果顯示,番茄葉片在經過空氣釋放MeJA後多酚氧化酵素(polyphenol oxidases, PPO)活性沒有顯著提升,而經過MeJA噴灑處理的葉片,PPO活性隨著處理後時間逐漸上升。而在經過M處理後番茄葉片的胰蛋白酶抑制劑(trypsin inhibitor, TI)的活性也沒有顯著上升,經過S處理的葉片活性在不同時間點,都有1.5倍以上的活性。處理過的番茄葉片餵食斜紋夜蛾幼蟲,幼蟲的相對生長速率(relative growth rate, RGR)在M處理後的24小時速率最低,而隨著時間經過之後生長速率會上升。在S處理在下,不同時間點RGR都有所下降,也隨著時間逐漸上升。總結本研究發現,空氣釋放處理未能提升PPO和TI之活性,然而在短時間內噴灑和空氣釋放皆可以降低昆蟲的生長速率。
When the plant was attacked by the insect, a large amount of MeJA accumulates in the plant. Then, the plant''s defense response, producing a lot of insect-resistant protein in the plant, to reducing the growth performance of the insect. Previous studies have found that spraying MeJA on plants directly induces insect-resistant responses to plants, and the treated leaves fed to Spodoptera litura can also reduce their growth rate. Because of current laws, direct spraying of chemicals is a category of pesticides. In addition, there are few experiments about the air release induce plants resistance. The purpose of this experiment is to understand the variations of plant defense-resistant proteins in tomato under air-release MeJA treatment (M) and spray MeJA(S) treatment; in addition, S. litura were fed with different treatments for the growth performance of tomato leaves. Results indicate that the activity of polyphenol oxidases (PPO) in tomato leaves after treating with air release MeJA is not significantly increased, while the leaves treated with spray MeJA are gradually increased with the treatment time. Then, the activity of trypsin inhibitor (TI) in tomato leaves did not increase significantly after M treatment, while the leaf TI activity of S-treated leaves had 1.5 times activity at different after treated time. The treated tomato leaves are fed with the larvae of S. litura. The relative growth rate (RGR) of the larvae was the lowest at 24 hours after M treatment and increases as the time goes. The RGR decreased at different timing during S treatment, and also increases as the time goes. In conclusion, the study found that air release MeJA cannot increase the activity of PPO and TI, however, both air released and sprayed MeJA can decrease the insect larvae growth rate.
URI: http://hdl.handle.net/11455/99409
文章公開時間: 2020-02-13
Appears in Collections:昆蟲學系

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