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dc.contributor.authorMing-Jie Linzh_TW
dc.contributor.authorYi-Ming Chenzh_TW
dc.contributor.authorChen-zhong Lizh_TW
dc.contributor.authorChing-Chou Wuzh_TW
dc.description.abstractAdalimumab (Ada) is widely used to treat autoimmune diseases, and a correct dose of Ada can maintain therapeutic effectiveness and relieve symptoms. This study develops a rapid quantifying method of Ada using magnetic nanoparticle (MNP)-based immunoreaction and disposable screen-printed carbon electrodes (SPCEs). Anti-Ada Fab fragment and mouse anti-human antibody is respectively used as primary antibody (Ab1) and secondary antibody (Ab2). Glucose oxidase (GOD) and the Ab2 were co-immobilized on silica nanoparticles (SiNPs) with the concentration ratio of 3:1 to promise good immune affinity and the more amount of immobilized GOD. After sequentially immunoreacting Ada with Ab1@MNPs and GOD-Ab2@SiNPs, the separated GOD-Ab2@SiNPs/Ada/Ab1@MNPs complex was dripped on the glucose/ferricyanide-coated SPCEs for GOD catalysis, and then the anodic current of reduced ferrocyanide was used for the quantification of Ada. The MNP-based immunoassays have good linearity from 0.1 μg/mL to 2 μg/mL and a limit of detection of 15.5 ng/mL. Moreover, the immunoassays can practically measure the Ada concentrations in 10-fold diluted patient serum samples with good recovery. The operation time of MNP-based immunoassays takes only about 0.5 h. The disposable MNP-based immunoassays have great promise to develop a rapid quantification platform for therapeutic drug monitoring.zh_TW
dc.subjectMagnetic nanoparticleszh_TW
dc.subjectGlucose oxidasezh_TW
dc.subjectSandwich immunoreactionzh_TW
dc.subjectDisposable electrodezh_TW
dc.subjectTherapeutic drug monitoringzh_TW
dc.titleElectrochemical sandwich immunoassay for quantification of therapeutic drugs based on the use of magnetic nanoparticles and silica nanoparticleszh_TW
dc.typejournal articlezh_TW
item.openairetypejournal article-
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Appears in Collections:生物產業機電工程學系
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