Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/10117
標題: 骨組織微結構與奈米機械性質之研究
Microstructures and Nanomechanical Properties of Skeletal tissues
作者: Hsiao, Hsiang-long
蕭翔隆
關鍵字: nanomechanical;奈米機械性質;casein;collagen fiber;酪蛋白;膠原纖維
出版社: 材料科學與工程學系所
引用: [1] O. Akkus, F. Adar, and M. B. Schaffler, “Age-related changes in physicochemical properties of mineral crystals are related to impaired mechanical function of cortical bone”, Bone, Vol. 34, p. 443- 453 (2004) [2] T. Hoc, L. Henry, M. Verdier, D. Aubry, L. Sedel, and A. Meunier, “Effect of microstructure on the mechanical properties of Haversian cortical bone”, Bone, Vol. 38, p. 466- 474 (2006) [3] J. Ge, F. Z. Cui, X. M. Wang, and H. L. Feng, “Property variations in the prism and the organic sheath within enamel by nanoindentation”, Biomaterials, Vol. 26, p. 3333-3339 (2005) [4] S. Habelitz, S.J. Marshall, G.W. Marshall Jr, and M.Balooch, “Mechanical properties of human dental enamel on the nanometre scale”, Arch. Oral. Biol., Vol. 46, p. 173-183 (2001) [5] L. C. Junqueira, J. Carneiro, and R. O. Kelley, “Basic Histology ninth edition”,合記圖書出版社,(2000) [6] Stavros C. Manolagas, and R. L. Jilka, “Bone Marrow, Cytokines, and Bone Remodeling”, N. Engl. J. Med., Vol. 332, No. 5, p. 305-311 (2006) [7] Turek SL. “Orthopaedics Principles and Their Applications”, J.B. Lippincott Company, Vol. 1, p. 31-100 (1984) [8] J.W. Ager III, G. Balooch, and R.O. Ritchie, “Fracture, aging, and disease in bone”, J. Mater. Res., Vol. 21, No. 8, p. 1878-1892 (2006) [9] H. Peterlik, P. Roschger, K. Klaushofer and P. Fratzl, “From brittle to ductile fracture of bone”, Nat. Mater., Vol. 5, p. 52-55 (2006) [10] D. Taylor, J. G. Hazenberg and T. C. Lee, “Living with cracks: Damage and repair in human bone”, Nat. Mater., Vol. 6, p. 263-268 (2007) [11] E. R.C. Draper, and A. E Goodship, “A novel technique for four-point bending of small bone samples with semi-automatic analysis”, J. Biomech., Vol. 36, p. 1497-1502 (2003) [12] S.P. Kotha, and N. Guzelsu, “Tensile damage and its effects on cortical bone”, J. Biomech., Vol. 36, p. 1683–1689 (2003) [13] X.M. Wang, F.Z. Cui, J. Ge, Y. Zhang, and C. Ma, “Variation of nanomechanical properties of bone by gene mutation in the zebrafish”, Biomaterials, Vol. 23, p. 4557-4563 (2002) [14] J. Y. Rho, P. Zioupos, J. D. Currey, and G. M. Pharr, “Variations in the Individual Thick Lamellar Properties Within Osteons by Nanoindentation”, Bone, Vol. 25, No. 3 (1999) [15] P. K. Zysset, X. E. Guo, C. E. Hoffler, K. E. Moore, and S. A. Goldstein, “Elastic modulus and hardness of cortical and trabecular bone lamellae measured by nanoindentation in the human femur”, J. Biomech., Vol. 32, p. 1005-1012 (1999) [16] M. J. Silva, and S. R. Ulrich, “In vitro sodium fluoride exposure decreases torsional and bending strength and increases ductility of mouse femora”, J. Biomech., Vol. 33, p. 231-234 (2000) [17] S. Hengsberger, A. Kulik, and Ph. Zysset, “Nanoindentation Discriminates the Elastic Properties of Individual Human Bone Lamellae Under Dry and Physiological Conditions”, Bone, Vol. 30, No. 1, p. 178-184 (2002) [18] J. Y. Rho, and G. M. Pharr, “Effects of drying on the mechanical properties of bovine femur measured by nanoindentation”, J. Mater. Sci. Mater. Med., Vol. 10, p. 485-488 (1999) [19] H.S. Gupta, U. Stachewicz, and W. Wagermaier, P. Roschger, H.D. Wagner, P. Fratzl, “Mechanical modulation at the lamellar level in osteonal bone”, J. Mater. Res., Vol. 21, No. 8, p. 1913-1921 (2006) [20] M. Vanleene, P. E. Mazeran, and M. C. Ho Ba Tho, “Influence of strain rate on the mechanical behavior of cortical bone interstitial lamellae at the micrometer scale”, J. Mater. Res., Vol. 21, No. 8, p. 2093-2097 (2006) [21] Z. Fan, and J. Y. Rho, “Effects of viscoelasticity and time-dependent plasticity on nanoindentation measurements of human cortical bone”, J. Biomed. Mater. Res., 67A, p. 208–214 (2003) [22] P. Zioupos and J. D. Currey, “Changes in the Stiffness, Strength, and Toughness of Human Cortical Bone With Age”, Bone, Vol. 22, No. 1, p. 57-66 (1998) [23] A. Xiang, M. Kanematsu, M. Mitamura, H. Kikkawa, S. Asano, and M. Kinoshita, “Analysis of Change Patterns of Microcomputed Tomography 3-Dimensional Bone Parameters as a High-Throughput Tool to Evaluate Antiosteoporotic Effects of Agents at an Early Stage of Ovariectomy-Induced Osteoporosis in Mice”, Invest. Radiol., Vol. 41, No. 9, p. 704-712 (2006) [24] Y. Zhang, F. Z. Cui, X. M. Wang, Q. L. Feng, and X. D. Zhu, “Mechanical Properties of Skeletal Bone in Gene-mutated Sto¨pseldtl28d and Wild-type Zebrafish (Danio rerio) Measured by Atomic Force Microscopy-based Nanoindentation”, Bone, Vol. 30, No. 4, p. 541-546 (2002) [25] M. A. Rubin, J. Rubin, and I. Jasiuk, “SEM and TEM study of the hierarchical structure of C57BL/6J and C3H/HeJ mice trabecular bone”, Bone, Vol. 35, p. 11-20 (2004) [26] S. Hengsberger, P. Ammann, B. Legros, R. Rizzoli, and P. Zysset, “Intrinsic bone tissue properties in adult rat vertebrae:modulation by dietary protein”, Bone, Vol. 36, p. 134-141 (2005) [27] G. Pelled, K. Tai, D. Sheyn, Y. Zilberman, S. Kumbar, L. S. Nair, C. T. Laurencin, D. Gazit, and C. Ortiz, “Structural and nanoindentation studies of stem cell-based tissue-engineered bone”, J. Biomech., Vol. 40, p. 399-411 (2007) [28] 郭卿雲,“畜產食品科技 另類的發酵乳 克弗爾”科學發展 [29] H. Hertz, and J. Reine, “On the Contact of Rigid Elastic Solids and on Hardness”, Verh Ver Beforderung Gewerbe Fleisses, Vol. 61, p. 410 (1881) [30] I. N. Sneddon, “The Relation Between Load and Penetration in the Axisymmetric Boussinesq Problem for a Punch of Arbitrary Profile”, Int. J. Eng. Sci., p. 47-56 (1965) [31] M.F. Doerner and W.D. Nix, “A method for interpreting the data from depth-sensing indentation instruments”, J. Matert. Res., Vol. 1, No. 4, p. 601 (1986) [32] W.C. Oliver and G.M. Pharr, “On the Generality of the Relationship Among Contact Stiffness, Contact Area, and Elastic Modulus During Indentation”, J. Mater. Res., Vol. 7, p. 613-617 (1992) [33] A.C. Fishcer-Cripps, Nanoindentation, Springer-Verlag, New York, 2002 [34] D. W. Dempster, R. Birchman, R. Xu, R. Lindsay, and V. Shen, “Temporal changes in cancellous bone structure of rats immediately after ovariectomy”, Bone, Vol. 16, No. 1, p. 157-161 (1995) [35] 陳家全、李家維、楊瑞森,「生物電子顯微鏡學」,行政院國家科學委員會精密儀器發展中心編印,第 23-37 頁。 [36] TriboScope User Manual, Hysitron Inc.
摘要: 
生物體硬組織 (如骨頭、牙齒等) 是由多層次奈米結構複合而成,釐清其機械性質對於仿生材料開發及相關醫學研究皆有相當大之助益。而骨頭結構細微且複雜,傳統巨觀下的機械分析技術已不再適用;因此,本研究以掃描式電子顯微鏡及奈米壓痕儀,進行鼠骨之微結構與機械性質之分析,釐清成鼠與年輕鼠、手術鼠以及骨質疏鬆經治療後之鼠股骨微結構與機械性質之差異。實驗結果發現,成鼠與年輕鼠緻密骨之微結構並無顯著差異,因磷酸鈣鹽沈積時間長短不同而致使成鼠之鈣磷含量略高於年輕鼠,因此成鼠之機械性質高於年輕鼠。手術鼠 (包括年輕鼠、假手術鼠、切除卵巢鼠) 組成成分大致相同,由骨組織之微結構照片可看到氫氧基磷灰石顆粒母相中有許多絲狀膠原纖維分佈其中。機械性質方面,假手術鼠緻密骨整體之機械性質高於切除卵巢鼠,由內側至外側之機械性質約保持定值,而切除卵巢處理則導致緻密骨內側發生骨質流失情形,使機械性質弱化。經治療 (發酵乳) 之小鼠,其膠原纖維之間排列緊密且無裂隙;在無治療的情況下,其膠原纖維的排列則顯得較為鬆散。另經發酵乳治療之鼠緻密骨內側無弱化情形,推論此發酵乳對於骨質疏鬆的治療較酪蛋白有效。

Biological hard tissues, such as bones and teeth, are composed of hierarchical nanostructures. To realize their mechanical properties will be helpful to development of biomimic materials and related medical research. However, since the structures of bones are fine and complicated, conventional macro-mechanical analyses are no longer suitable. Therefore in this study, the microstructures of mouse bones before and after medical treatments were investigated by scanning electron microscopy, and the nanomechanical properties were measured by nanoindentation tests. The cortical bones of mature mice were porous structures which were the same as those of young mice. The calcium/phosphorous contents of mature mice were higher than those of young mice due to the deposition time of hydroxyapatite. Thus the mechanical properties of the mature mice were higher than those of young mice. The compositions of operated mice (inclusing young mice, sham mice and ovariectomy) were similar. From the microstructures of bone tissues, many collagen fibers were observed to disperse in the hydroxyapatite matrix. Besides, the mechanical properties of the cortical bones of sham mice were higher than ovariectomy mice. The mechanical properties from inside to outside of the cortical bone were consistent for young mice and sham mice. However, they decreased for ovariectomy mice due to osteoporosis. Moreover, the mice with Kefir nutriment treatment were observed that the collagen fibers arranged into a compact structure and no crack was observed. However, under an untreated condition, the structures revealed loose and disordered. The mechanical properties of cortical bones after Kefir treatment, were the same as those of normal mice. In addition, Kefir is more effective than Casein to osteoporosis treatment.
URI: http://hdl.handle.net/11455/10117
其他識別: U0005-0308200621545800
Appears in Collections:材料科學與工程學系

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