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標題: 鼠緻密骨組織奈米尺度機械性質與變形行為之研究
Nanomechanical Properties and Deformation Behaviors of Cortical Bone Tissue of Mice
作者: 王盈婷
Wang, Ying-Ting
關鍵字: bone;骨骼;mechanical property;deformation;機械性質;變形
出版社: 材料科學與工程學系所
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骨骼為天然層狀結構,釐清其內部各微細結構與變形行為之間的關連性,對於骨骼病理學之研究以及生醫材料之開發皆有相當大的助益。因此本研究採用假手術鼠與切除卵巢鼠,透過高解析 X 光微型電腦斷層掃瞄及掃描式電子顯微鏡,分析鼠脛骨樣貌、骨礦物質密度及微結構;並以場發射穿透式電子顯微鏡觀察緻密骨氫氧基磷灰石晶體結構,比較老化對骨骼晶體結構上的差異。同時以奈米壓痕儀對鼠骨之機械性質與變形行為進行探討,探討緻密骨韌化機制與裂隙擴展行為,嘗試釐清在原子尺度下骨骼結構與變形行為之間的相關性;最後以同步觀測釐清在壓痕測試下裂隙擴展與骨骼結構彼此交互作用之情形。

To clarify the relation between the microstructures and deformation behaviors of hierarchical bone structures will benefit the research of skeletal pathology and the development of biomedical materials. Thus in this study, the morphologies, mineral densities and microstructures as well as the arrangement of hydroxyapatite and collagen fibers of sham-operated and ovariectomized mouse cortical bone were examined by micro-computed tomography and electron microscopy. The mechanical properties and deformation behaviors of the mouse bone were analyzed by nanoindentation to clarify the toughening mechanisms and crack propagation behaviors as well as to realize the nanoscaled deformation of bone. By in-situ nanoindentation / TEM observations, the interactions between bone structures and crack propagations were verified.
The sham-operated mouse bone was dense, composed of aligned collagen fibers and hydroxyapatite crystals, and had a high bone mineral density and good mechanical properties. The toughening mechanisms included crack deflection, ligament bridging and microcracking. At the atomic scale, intergranular fracture, lattice distortion and dislocation formation were observed. On the contrary, due to ostreoporosis caused by decreased estrogen, the ovariectomized mouse bone was loose with a random arrangement of hydroxyapatite crystals and collagen fibers, thus lowering the mechanical properties. Sharp cracks propagated straight with a few collagen fiber bridging. Intergranular fracture, grain sliding and rotation, lattice distortion and dislocation formation were observed.
其他識別: U0005-1706201118364900
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