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標題: 2009-2011年間台灣鸚鵡披衣菌之盛行率調查及鸚鵡披衣菌之主要外膜蛋白基因序列分析
The prevalence of Chlamydophila psittaci and Alignment Analysis of Major Outer Membrane Protein Gene in Taiwan between 2009 and 2011
作者: 劉思妤
Liu, Szu-Yu
關鍵字: Chlamydophila psittaci;鸚鵡披衣菌;waterfowl;paittacine bird;wild bird;水禽類;鸚鵡鳥類;野生鳥類
出版社: 獸醫學系暨研究所
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鸚鵡披衣菌為絕對細胞寄生性之格蘭氏陰性細菌,目前已知可以感染來自於30個目的467個鳥種,依據其主要外膜蛋白(Major outer membrane protein;MOMP)的ompA(Outer membrane protein A)基因序列分析,可以將鸚鵡披衣菌分成9個基因型,分別為基因型A - F、E/B、M56及WC。而近年來在各國間分別出現人類接觸鸚鵡鳥類、鴿子及鴨隻而感染鸚鵡披衣菌的病例出現,顯示在公共衛生方面有必要瞭解台灣各個鳥種間的鸚鵡披衣菌盛行率。本調查研究以水禽類、鸚鵡鳥類、鳩鴿科鳥類及野生鳥類為主,進行鸚鵡披衣菌的盛行率調查以及其基因型的分析,結果在2010年間水禽類的盛行率為12.7%(146/1148),而18株鸚鵡披衣菌經基因型分析結果發現序列分為兩個部分,且皆為非典型基因型的鸚鵡披衣菌;而2010年1月至2011年6月間鸚鵡鳥類的盛行率為3.1%(16/514),其中9株鸚鵡披衣菌的基因型皆為genotype A;而2009年1月至2011年6月間鳩鴿科鳥類的盛行率為10.1%(8/79),而野生鳥類的盛行率為2.2%(14/650),當中4株鳩鴿科鳥類的鸚鵡披衣菌基因型皆為genotype B,然而在野生鳥類中1株黑冠麻鷺及1株五色鳥的樣本無法進行鸚鵡披衣菌的基因型分析,因此僅分析ompA之部分序列VDI(Variable domain I),結果發現兩株序列皆為非典型披衣菌種別。本調查研究顯示台灣的水禽類、鸚鵡鳥類、鳩鴿科鳥類及野生鳥類都有鸚鵡披衣菌的病原存在,其中鸚鵡鳥類及鳩鴿科鳥類的鸚鵡披衣菌基因型在國外皆曾出現人被感染的病例,因此在公共衛生上是值得重視的議題;此外在水禽類、黑冠麻鷺及五色鳥出現非典型的披衣菌,皆需進一步探討其病原性以評估這類非典型的披衣菌在各種鳥種族群中扮演的角色,然而在尚未確立前仍然不容忽視非典型的披衣菌在公共衛生上的威脅性。

Chlamydophila psittaci (C. psittaci), an obligate intracellular bacterium which causes avian chlamydosis in 467 avian species and 30 orders, can be divided into nine genotypes (A to F, E/B, M56, and WC) according to its outer membrane protein A (ompA) gene. A number of reports have demonstrated that humans can be infected C. psittaci via contacting infected psittacine pet birds, pigeons, and domestic waterfowls. Therefore, the current prevalence status of C. psittaci in birds in Taiwan is critical for public health. In the current study, we investigated the prevalence of C. psittaci in domestic waterfowls, psittacine birds, columbidae birds and non-psittacine birds in Taiwan. The positive rate of C. psittaci in domestic waterfowls was 12.7% (146/1148) in 2010. In psittacine birds, 3.1% (16/514) of samples were positive for C. psittaci between January 2010 and June 2011. In columbidae birds and non-psittacine birds, the positive rates of C. psittaci were 10.1% (8/79) and 2.2% (14/650), respectively. Genotype A was detected in 9 psittacine birds, and genotype B were detected in 4 columbidae birds. However, the phylogenetic analysis of partial ompA gene from 18 domestic waterfowl strains revealed that there were two atypical genotype clusters outside the currently recognized genotypes of C. psittaci. Furthermore, two atypical strains of Chlamydiaceae were detected by ompA variable domain I (VDI) sequence analysis from one Gorsachius melanolophus and one Megalaima nuchalis. To sum up, the results indicated that C. psittaci-infected psittacine birds and columbidae birds may be a potential risk factor for transmitting the disease from pet birds and pigeons to humans. Further studies need to be done for the pathogenesis of these atypical Chlamydiaceae in different avian species and the investigation of their capability to cause public health issue.
其他識別: U0005-1101201215251900
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