Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/12896
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dc.contributor.advisor謝快樂zh_TW
dc.contributor.advisorXie, Kuai-Yueen_US
dc.contributor.author夏懷安zh_TW
dc.contributor.authorXia, Huai-Anen_US
dc.date1988zh_TW
dc.date.accessioned2014-06-06T06:49:58Z-
dc.date.available2014-06-06T06:49:58Z-
dc.identifier.urihttp://hdl.handle.net/11455/12896-
dc.description.abstract傳染性華氏囊炎病毒D -78株,分別以雞胚胎和組織培養法增殖,再經10%PEG 6000和20∼50%連續蔗糖梯度純化,在35%處有病毒層,用陰染法在電子 顯微鏡下觀察,病毒大小約50∼60um,六角形。 以純化病毒,君上佐劑,免疫BALB/C 鼷鼠腹腔和皮下補強兩次,由眼窩或尾靜脈採 血,作瓊脂免疫擴散沈澱法(AGP )測定抗體力價,若有正反應,則靜脈補強抗原0 .1ml,3∼4天後製備融合瘤。 融合時,用SP-2和老鼠脾臟細胞,在40%PEG 1000的輔助下融合,以HAT 培 養基篩選有脾臟細胞和SP-2細胞雙重特性的融合瘤,10∼14天後,以酵素結合 免疫吸附法(ELISA )篩選有抗體分泌能力的融合瘤,再經極限稀釋法(limiting d -ilution)單株化,一共得到11株分泌單源抗體的融合瘤。 取其中三株生長快速和穩定的融合瘤,分別命名A -1、A -2、A -3大量增殖, 且以腹水法取得大量單源抗體,以供定性。 以間接螢光染色法(IFA )測定,均與D -78株,有特異性反應,而中和試驗,均 呈陰性反應,顯示此三株抗體均無中和能力。zh_TW
dc.language.isoen_USzh_TW
dc.publisher獸醫研究所zh_TW
dc.subjectAGPen_US
dc.subject華氏囊炎zh_TW
dc.subjectELISAen_US
dc.subjectVETERINARYen_US
dc.subjectANIMAL-SCIENCEen_US
dc.subject病毒zh_TW
dc.subject抗體zh_TW
dc.subject單源抗體zh_TW
dc.subject雞胚胎zh_TW
dc.subject組織培養法zh_TW
dc.subject瓊脂免疫擴散沈澱zh_TW
dc.subject酵素結合免疫吸附zh_TW
dc.subject獸醫zh_TW
dc.subject動物學zh_TW
dc.title雞傳染性華氏囊病單源抗體生產細胞之建立及被動免疫之研究zh_TW
dc.titleProduction of Monoclonal Antibodies Against Infectious Bursal Disease Virus and Studies on Passive Immunity of The Virnsen_US
dc.typeThesis and Dissertationzh_TW
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
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