Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/12992
標題: 安默西林在日本鰻之殘留檢測與藥物動力學研究
Amoxicillin residue detection and its pharmacokinetics study in the Japanese eel, Anguilla japonica
作者: 詹鈞陽
Chan, Chun-Yang
關鍵字: Amoxicillin;安默西林;pharmacokinetic;residue detection;藥物動力學;殘留檢測
出版社: 獸醫學系暨研究所
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摘要: 
本研究之目的在於建立一套精準的amoxicillin檢測方法,並用於測定口服投予40和80 mg/kg B.W./day amoxicillin於鰻魚後限定時間內,在血液中藥物動力學研究與組織殘留量。本研究選用磷酸溶液萃取組織中的amoxicillin,以固相萃取匣 (SPE) 萃取、淨化及濃縮,再添加trichloroacetic acid (TCA) 及甲醛 (formaldehyde) 後,以100 ℃水浴30 分鐘進行衍生化。選用的分析條件為:Cosmosil 5C18-MS之逆相層析管柱(5 µm, 4.6 X 150 mm i.d.; Nacalai, Kyoto, Japan);移動相為25 mM K2HPO4 (pH 8.5) : ACN (85 : 15, v/v);螢光檢測器的激發波長為358 nm,散發波長為440 nm。研究結果顯示,amoxicillin滯留時間約為4分鐘,檢測極限(LOD)為0.01 ppm,組織萃取平均回收率為82~106%之間。單一劑量40 mg/kg B.W./day 投予amoxicillin於鰻魚後,血液藥物動力學參數結果如下:T1/2為5.93 ± 0.06 hr,Tmax為3 ± 0 hr,Cmax為7.03 ± 0.12 mg/kg,MRT為9.4 ± 0.13 hr,Cl為884 ± 13 mL/hr-kg,AUCinf為45 ± 0.67 hr × μg/mL。此外藥物殘留試驗實驗結果顯示肌肉及肝臟低劑量組(40 mg/ kg-bw)第 1天時可測得平均濃度為180 與260 ppb,2天時可測得平均濃度下降為70與110 ppb,給藥後第三天皆低於檢測極限30 ppb。高劑量組(80 mg/ kg-bw) 第 1天時可測得平均濃度為930 與1,070 ppb,3天時可測得平均濃度下降為35 ppb 與 95 ppb,給藥後第五天無論是肌肉或是肝臟組織均低於檢測極限。

The aims of this study are to develop an accurate method for determination of tissue residual levels of eel fed with amoxicillin (AMX). AMX was extracted from various tissues of eel with phosphate buffer solution by solid-phase extraction. Extracted samples were subjected to derivatization by trichloroacetic acid (TCA) and formaldehyde in boiling water for 30 mins. HPLC separation of AMX was carried out on a Cosmosil 5C18-MS column with mobile phase of 25 mM K2HPO4 (pH 8.5) and acetonitrile at a ratio of 85 : 15 (v/v). The excitation and emission wavelength are 358 nm and 440 nm, respectively. The results revealed that the recoveries of muscle, liver, and serum from eel were 84.7-105.1%, 81.8-104.9%, and 88.6-106.3%, respectively. The tested eels were separated as two groups treated 40 and 80 mg/kg bw/day of amoxicilin following oral administration, respectively. A peak with retention time around 4 min was observed. After given single dose 40 mg/kg bw/day of amoxicillin, via oral administration the pharmacokinetic parameters T1/2, Tmax, Cmax, MRT, Cl and AUCinf were 5.93 ± 0.06 hr, 3 ± 0 hr, 7.03 ± 0.12 ppm, 9.4 ± 0.13 hr, 884 ± 13 mL/hr-kg and 45 ± 0.67 hr × μg/mL, respectively. In addition, our results also showed that the average drug residue concentration in muscle and liver of the low dose group (40 mg / kg-bw) in the first and second day after administration was (180 and 260 ppb) and (70 and 110 ppb), respectively. However, both residue concentrations in muscle and liver of the group in the third day after administrations were under were below the detection limit (lower 30 ppb). Furthermore, our results indicated that the average drug residue amount in muscle and liver of the high dose group (80 mg / kg-bw) in the first and third day after administration was (930 and 1,070 ppb) and (35 and 95 ppb), respectively. Besides, both residue amounts in muscle and liver of the group in the fifth day after administration were under the detection limit.
URI: http://hdl.handle.net/11455/12992
其他識別: U0005-2206201120580600
Appears in Collections:獸醫學系所

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