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標題: 領角鴞糞中醣皮質酮年週期變動之研究
Annual pattern of fecal corticoid excretion in Collared-Scope owls
作者: 鍾岡樺
Chung, Kang-Hua
關鍵字: Collared-Scope owls;領角鴞;fecal corticoid;Non-invasive;corticosterone;醣皮質酮;非侵入性;糞便
出版社: 獸醫學系暨研究所
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領角鴞為台灣特有亞種,屬於珍貴稀有保育類。由於其生活習性和棲地與人類的活動範圍相近,因此領角鴞常因意外或人為干擾而成為救傷中心的常客。許多研究報告指出,緊迫的狀態會造成許多生理性的傷害,但對於評估領角鴞的緊迫狀態的研究則較少。本實驗應用非侵入之分析法檢驗領角鴞糞中醣皮質酮濃度,並建立一個年週期波動資料,以期提供繁殖時參考用。我們在2007年至2008年期間利用收容的10隻領角鴞分為六組實驗,四組配對組、兩組單獨籠飼,共收集糞便樣本576個。樣本萃取後以EIA酵素免疫分析法檢測醣皮質酮濃度。結果顯示所有個體的全年度醣皮質酮濃度變動在11月時濃度最低(353.91 ± 25.90 ng/ml);在3月時濃度最高(537.03 ± 40.40 ng/ml),次高峰濃度在12月時出現(501.74 ± 56.93 mg/ml),最高濃度與全年平均(443.83 ± 13.78 ng/ml)或最低濃度比較皆有顯著和極顯著的差異(p < 0.05;p < 0.01)。此結果與領角鴞行為觀察之產蛋育雛期主要在3至4月或求偶配對期在11至12月等現象一致,顯示糞便中醣皮質酮濃度檢驗法可反映領角鴞全年的繁殖生理與行為之變化。在季節波動分析發現所有個體的醣皮質酮濃度在春季時最高(529.46 ± 39.90 ng/ml),極顯著的較秋季高(366.90 ± 29.34 ng/ml, p < 0.01),由此結果推測領角鴞的繁殖季主要集中在春季;而秋季時可能因為較低的生理需求和環境較為舒適,故醣皮質酮濃度較低。繁殖季時之糞醣皮質酮濃度(486.75 ± 21.51 ng/ml)極顯著較非繁殖季之濃度(413.79 ± 17.18 ng/ml)高(p < 0.01),此證實動物體在繁殖季時需克服競爭而有較高的生理需求和繁殖行為上的緊迫壓力。另外,不同性別個體在醣皮質酮的濃度變動上仍具有相關性(四季,r = 0.9288;全年,r = 0.7604);配對處理的影響則在四季或全年的濃度變化上不具有顯著影響,與未配對的組別仍具有近高度的相關性(配對與未配對r > 0.58),並且仍須要修正研究方法來排除動物隻數和樣品數不足的問題。本研究所建立之全年度糞醣皮質酮濃度波動變化資料可應用在領角鴞的生態調查或復育計畫上之參考。

The collared-scope owls are the endemic subspecies of Taiwan and it was classified as rare valuable species. There living habit and habitat near to human life, so they become the common species of the wildlife rescue center. It''s well-known that stressful conditions would cause several harmful physiological conditions, our study is to evaluate the stress condition of these at-risk species. The purpose of present study is to investigate the fecal corticosterone metabolites (FCM) level of the collared-scope owls by the noninvasive methods, and established a profile of the annual pattern of fecal corticoid excretion. We used ten collared-scope owls to classified into six groups, four groups were couples, two groups were singtons. Totally 576 fecal samples were collected during 2007 to 2008, which were extracted then measured by the enzyme immunoassay (EIAs). The results of the annual pattern of FCM suggested that November had the lowest mean FCM (353.91 ± 25.90 ng/ml) while March had the highest (537.03 ± 40.40 ng/ml), and the second peak occurred around December (501.74 ± 56.93 ng/ml). Furthermore, the corticosterone level of March was significantly higher than the annual mean level(443.83 ± 13.78 ng/ml, p < 0.05) and was vary significantly higher than the month of the lowest level(p < 0.01). These results correlated with the timing of eggs laying occurred predominantly in March to April, while the period of courtship and copulation occurred in December toward January. The FCM also fluctuated with the seasons, spring had the highest FCM (529.46 ± 39.90 ng/ml) and it was vary significantly higher than autumn (the lowest, 366.90 ± 29.34 ng/ml, p < 0.01). We supposed that our collared-scope owls were breeding predominantly during spring, they might also have lower physiological and environmental stress during autumn. Moreover, Collared-scope owls had vary significantly higher level of FCM in breeding season (486.75 ± 21.51 ng/ml), compare to the Non-breeding season(413.79 ± 17.18 ng/ml, p < 0.01). it correspond to that animals have more physiological needs to overcome the challenges of competition and reproduction. Besides, it was correlation between genders (seasonal pattern, r = 0.9288; annual pattern, r = 0.7604) and the results of the couples compared to the singletons were similar on the FCM deviation in males and females in different comparison of pattern (each r > 0.58), and we need to modify the methods of the study to solve the problem of that the sample size is not enough. According to our results, the analysis of FCM is appropriate for assessing the changes of physiology status and reflecting the occurrence of breeding behavior on them. The annual profile of the FCM could be a reference for research and restoration purposes on the collared-scope owls.
其他識別: U0005-2508201110225500
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