Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13264
標題: 應用間接酵素連結免疫吸附法(ELISA)檢測家禽沙氏桿菌症
Development and evaluation of indirect Enzyme-Linked Immunosorbent Assay(ELISA) for detecting avian salmonellosis
作者: 盧文鴻
Hong, Lu Wen
關鍵字: Avian Salmonellosis;間接酵素連結免疫吸附法;Lipopolysaccharide Antigen;Flagella Antigen;家禽沙氏桿菌症;脂多醣抗原;鞭毛抗原
出版社: 獸醫學系
摘要: 
以三種 LPS 抗原檢測 900 個種雞血清樣本, S.pullorum LPS 抗原單
獨檢出陽性比例為 7 ﹪ (63/900) , S.enteritidis LPS 抗原單獨檢
出陽性比例為 5.2 ﹪ (47/900) , S.typhimurium LPS 抗原單獨檢出
陽性比例為 0.22 ﹪ (2/900) , 其中 S.pullorum LPS 抗原與 S.
enteritidis LPS 抗原的重複檢出陽性比例高達 75 ﹪ (675/900) , S.
pullorum LPS 抗原與 S.typhimurium LPS 抗原重複檢出陽性比例為
31.67 ﹪ (285/900) , S.enteritidis LPS 抗原與 S.typhimurium
LPS 抗原重複檢出陽性比例為 31.56 ﹪ (284/900) 。 由此可見,同屬
於 D1 血清群的 S.pullorum 和 S.enteritidis LPS 抗原彼此間有較明
顯的交叉反應產生, 而與屬於 B 血清群的 S.typhimurium LPS 抗原之
間的交叉反應則較不明顯。 三種 LPS 抗原重複檢出陽性的 282 個血清
樣本, 以兩種鞭毛抗原篩檢結果, S.enteritidis 和 S.typhimurium
鞭毛抗原檢出陽性率分別為 95.39 ﹪ (269/282) 、 94.33 ﹪
(266/282) ,重複檢出率為 91.84 ﹪ (259/ 282) ,顯示鞭毛抗原之間
的交叉反應十分明顯。

When the estabished ELISA antigen plates were used to
detect 900 field serum samples ,675 out of 900(75 ﹪ )were
positive in both S.pullorum and S.enteritidis
lipopolysaccharide antigens (LPS Ag) , 285 out of 900(31.67 ﹪
) were positive in both S. pullorum and S. typhimurum LPS Ag
and 31.56 ﹪ (284/900) in both S.enteritidis and S.typhimurium
LPS Ag, whereas 63 out of 900 (7 ﹪ ) were positive in
S.pullorum LPS Ag only, 47 out of 900 (5.2 ﹪ ) in
S.enteritidis LPS Ag only.It was noted that the rate of cross
reaction within the same group. There were 282 serum samples
which were ELISA positive in all three kinds of
lipopolysaccharide antigen. Those sera were subjected to the
ELISA tests using flagella antigen from S. enteritidis or
S.typhimurium. the positive rate was 95.39 ﹪ (269/ 282) in
S.enteritidis flagella antigen only, 94.33 ﹪ (266/282) in
S.typhimurium flagella antigen only, and 91.84 ﹪ (259/282) in
both flagella antigen. It is evident that there are strong
cross reaction in ELISA tests using flagella antigens.
URI: http://hdl.handle.net/11455/13264
Appears in Collections:獸醫學系所

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