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The Activity of Endometrial Matrix Metalloproteinase (MMP) -2 and MMP-9 and the Level of Plasma Endotoxin and Vasopressin in Dogs with Pyometra
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子宮積膿症是中老齡未結紮母犬常見的疾病之ㄧ，本病常顯現出許多與細菌性感染以及炎症反應有關的系統性症狀，這些系統性症狀分別與炎症反應、血中內毒素與血管加壓素濃度有著一定的相關性。其中炎症反應可能使患犬子宮內膜上的基質金屬蛋白酶（matrix metalloproteinase, MMP）2（MMP-2）與9（MMP-9）上升。MMP是一種可以分解細胞外基質的酵素，是參與子宮內膜更新與炎症反應進行的重要因子。為了解MMPs、內毒素濃度、血管加壓素以及感染菌株間之關連性，本研究分別測定犬隻血漿與子宮內膜上的MMP-2與MMP-9表現量、血漿中內毒素與血管加壓素之濃度，並配合細菌學的檢查比較各因子之間之差異。結果發現子宮積膿患犬於血漿與子宮內膜上之MMP-2與MMP-9活性，顯著的高於子宮內膜囊腫性增生犬隻與對照組犬隻；子宮積膿患犬血漿中血管加壓素之濃度亦顯著的高於對照組犬隻，但血漿中內毒素濃度則與對照組犬隻無明顯差異，而致病菌的種類差異不會使血漿和子宮內膜上之MMP-2與MMP-9以及內毒素濃度產生顯著的差異。由本研究結果可知應有其他因子影響著子宮積膿患犬血漿與子宮內膜上MMP-2和MMP-9之表現，未來必須進行進一步的研究以釐清其他影響MMP-2與MMP-9在子宮積膿患犬之因子
Pyometra is one of the common diseases in middle-aged intact bitches. This disease is often manifested as systemic illness related to the bacterial infection and inflammatory response. Recent study indicate the higher activities of MMP-2 and MMP-9 in bitches with pyometra may cause by the inflammation response. It is known that endotoxin level in plasma is significantly increased in bitches with pyometra and may be responsible for the systemic symptoms associated to the inflammation in affected dogs. However, the relationship between MMPs and endotoxin level were still unknown. In this study, we examined the MMPs activity in endometrium from 78 bitches and plasma of 47 bitches by zymography. The results of this study indicated that the activities of MMP-2 and MMP-9 of endometrium and plasma in bitches with pyometra were significantly higher than those of normal bitches (P<0.01). The vasopressin concentration in dogs with pyometra were significantly higher than those of normal bitches (P<0.01). However, there was no significant difference of plasma endotoxin level between dogs with pyometra and normal dogs. The MMP-2 and MMP-9 activity and endotoxin didn't show significant difference between bitches affected by Gram-negative and Gram-positive bacteria. In conclusion, the high MMP-2 and MMP-9 activity may indicate the serious inflammation response in bitches with pyometra. However the endotoxin level and bacteria strain didn't influence the MMP-2 and MMP-9 level in dogs with pyometra. The results presented the high MMP-2 and MMP-9 activity in dogs with pyometra which may influenced by many factors. Thus, further investigation concerning the influence factor of MMP-2 and MMP-9 activity in dogs with pyometra is warranted.
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