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The activity of MMP-2 and MMP-9 in plasma and endometrium of dogs during estrous cycle
|關鍵字:||canine;狗;MMP-2;MMP-9;estrous cycle;plasma;endometrium;基質金屬蛋白酵素2;基質金屬蛋白酵素9;動情週期;血漿;子宮內膜||出版社:||獸醫學系暨研究所||引用:||夏偉堯。犬子宮積膿之荷爾蒙、臨床病理學、子宮菌相與血漿中基質金屬蛋白酵素2與9的變化。碩士論文。中華民國國立中興大學。台中市。台灣。2004。 吳冠倫。子宮內膜基質金屬蛋白酵素2與9之活性與血漿中內毒素及血管加壓素濃度於子宮積膿犬隻之變化。碩士論文。中華民國國立中興大學。台中市。台灣。2007。 Agah A, Kyriakides TR, Letrondo N, Bjorkblom B and Bornstein P. Thrombospondin 2 levels are increased in aged mice: consequences for cutaneous wound healing and angiogenesis. Matrix Biol. 22, 2004. Ancelin M, Buteau-Lozano H, Meduri G, Osborne-Pellegrin M, Sordello S, Plouët J, Perrot-Applanat M.A . Dynamic shift of VEGF isoforms with a transient and selective progesterone-induced expression of VEGF189 regulates angiogenesis and vascular permeability in human uterus. Proc Natl Acad Sci .30;99(9):6023-8.2002 Basbaum CB, Werb Z. Focalized proteolysis: spatial and temporal regulation of extracellular matrix degradation at the cell surface. Curr Opin Cell Biol. 8:731–8, 1999. 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基質金屬蛋白酵素（matrix metalloproteinases, MMPs）為一功能性蛋白酵素，不同的基質金屬蛋白酵素能降解細胞外基質，改變組織型態。人類女性月經週期變化中，子宮內膜組織有顯著地增加或減少，同時觀察到也會在不同時期顯著地增加或是消失。這些特殊的MMPs在月經週期裡調控子宮內模組織的斷裂和重塑。在產後的母犬子宮組織中，MMP-9擔負起子宮復舊的重要角色。本研究主題在於自然環境下犬之子宮內膜及血漿中MMPs變化和血液荷爾蒙的相關性。我們採取被施行子宮卵巢摘除手術之健康母犬術前及術後的血液和子宮卵巢組織，經過萃取和離心樣本後，利用sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE)偵測子宮內膜及血漿中MMP-2及MMP-9活性，並偵測血漿中雌二醇和黃體酮的濃度。結果顯示術前血漿proMMP-2和MMP-9活性變化會隨著雌二醇濃度不同而改變，且術前血漿proMMP-2和MMP-9和雌二醇濃度有顯著的正相關(p<0.05)。同時，發情前期術前血漿MMP-2的活性表現顯著高於無發情期和晚期發情間期(p<0.05)。而在早期發情間期，子宮內膜MMP-和MMP-2的活性表現顯著高於發情前期和發情期(p<0.05)。儘管子宮內膜MMP-2和MMP-9的活性和雌二醇、黃體酮濃度呈現負相關，但彼此的相關程度十分微弱。最後，應用zymography能偵測犬在發情周期中，血漿以及子宮內膜MMP-9和MMP-2的活性表現，並探討其變化。本研究結果顯示雌二醇和黃體酮間接透過其他路徑影響子宮內膜基質細胞表現MMP-9和MMP-2，而血漿MMP-2和MMP-9與雌二醇分泌的相關性能反映雌二醇影響細胞分泌MMP-2和MMP-9，影響血漿中MMP-2和MMP-9的含量。
Esrus cycle of human menstruation, the endometrium shows a significantly increment of the expression of a matrix metalloproteinases (MMPs). Several MMPs are involved in the regulating the breakdown and the remodeling of the endometrium throughout the menstrual cycle. Despite, in the postpartum dog, MMPs are involved with uterine remodeling and MMP-9 activities during placentation extend in the endometrium. The aim of this study was to observe the endometrium and plasma activity of matrix metalloproteinases and hormones during different estrus phase. Blood samples and uterine were collected from healthy bitches while before and after ovariohysterectomy. After extraction and centrifuge, the endometrium and plasma activity of MMP-2 and MMP-9 determined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE) and the concentrations of estradiol and progesterone were detected, too. The results of this study indicated plasma proMMP-2 and totalMMP-9 activity before surgery change with the estrodiol change. The activity of proMMP-2 and MMP-9 were positive correlation with the estrodiol (p<0.05). Likewise, plasma MMP-2 in proestrous period before surgery was significantly higher than anestrous and late-metestrous (p<0.05). Otherwise, the activity of MMP-9 and MMP-2 in endometrium are higher in early metestrous than in proestrous and estrous (p<0.05). The correlation between endometrial MMP-2, 9 and estrodiol is negative. And the correlation between endometrial MMP-2, 9 and progestrone is negative, too. Nevertheless, the correlation strength of them is weak. In conclusion, the activity of plasma and endometrial MMP-2, 9 can be detected by zymography in estrous cycle. The result indicated E2 and P4 may not affect endometrial stoma cell directly, but plasma MMP-2 and MMP-9 were associated with estrogen secretion which can reflect the signal transferring expression of MMPs.
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