Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13800
標題: Sodium diethyldithiocarbamate、靈芝及interleukin-2對小白鼠脾臟細 胞免疫活性的影響
The influence of sodium diethyldithiocarbamate, Ganoderma and interleukin-2 on splenocytes activity of mice
作者: 葉修如
Yea, Shiou Ru
關鍵字: Ganoderma;靈芝;sodium diethyldithiocarbamate;interleukin-2;MTT;白血球間素-2;MTT呈色法;免疫促進劑
出版社: 獸醫學系
摘要: 
在活體外試驗,靈芝(含葡萄糖量0.012-192 ug/ml)對小白鼠脾臟細胞及
yac-1細胞不具增殖及細胞毒性作用,DTC於0.98 ng/ml至8 ng/ml具毒殺
組織細胞的作用,而0.49 ng/ml以下不具細胞毒性,IL-2於2.93 units/
ml至3000 units/ml細胞的增殖作用隨劑量增加而升高。在活體試驗方面
,DTC (50 mg/kg b.w.)腹腔注射7天後,使脾臟細胞對5% PHA及5 ug/ml
LPS反應增強,各為對照組的302%及116%,注射14天後,仍有增強
PHA(182%)及LPS反應(175%)。靈芝(含葡萄糖量3 mg/隻)注射後一週,
對2 ug/ml Con A、PHA及LPS反應皆未增強。以靈芝飼料 (1.6 g/kg b.
w./day)餵飼22週測定,對Con A反應有增強作用(158%)。以IL-2
(6-9x103 units/kg b.w.)注射7天後,能增強脾臟細胞對PHA (369%)及
LPS (478%)的反應。NBT染色結果, DTC、靈芝(注射及餵食)及IL-2皆
對脾臟細胞吞噬活性有增強作用。

The immunomodulatory potential of sodium diethyl-
dithiocarbamate (DTC), Ganoderma extract and interleukin-2
(IL-2) was assessed in vivo and in vitro study. For in vitro
study, the tetrazolium salt colorimetric assay (MTT) was
performed to evaluated cell proliferation. The result showed
that DTC and Ganoderma extract did not enhance the
proliferation induced by concanavalin A (Con A, 2 ug/ml). In
fact, the DTC (0.98-7.8 ng/ml) was toxic (>80% cell dead) to
splenocytes and yac-1 cells in culture. The presence of
Ganoderma extract did not demonstrate the inhibitory effect on
splenocytes and yac-1 cells. For in vivo study, mice were
treated with DTC (50 mg/kg b.w., i.p.) once and splenocytes
were prepared on 7 or 14-day after treatment. The splenocytes
of DTC-treated animals were showed an increase in mitogen-
induced proliferation in vitro. The values of %proliferation
were 100% for control group, 302% for phytohemagglutinin
(5%)-7-day group, 116% for lipopolysaccharide (5 ug/ml)-7-day
group, 182%for PHA-14-day group and 175% for LPS-14-day group.
Treatment with Ganoderma extract (3 mg glucose content/mouse ,
i.p.) did not enhance the splenocyte proliferation induced by
mitogen (Con A, PHA and LPS). However, mice fed with feed
containing Ganoderma powder (1.6 g/kg b.w./day) for 22 wk were
showed an enhancement of Con A (2 ug/ml)-induced proliferation
(158%). Five-day IL-2 treatment (6-9x103 units/mouse, i.p.)
resulted in enhancement of PHA response (369%) and LPS response
(478%). NK activity of mice treated with DTC, Ganoderma and
IL-2 (i.p.) was measured with LDH release assay. Because of
the high spontaneous release of effector cells (splenocytes),
the result was not suitable to judge the effect of DTC,
Ganoderma on NK activity. However, these substances did
slightly increase the NK activity of treatment group. In
addition, the phagocytic activity assessed with NBT stain was
enhanced by the treatment with DTC, Ganoderma and IL-2 .
URI: http://hdl.handle.net/11455/13800
Appears in Collections:獸醫學系所

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