Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13968
標題: 比較脂肪幹細胞與骨髓基質幹細胞對於兔子椎間盤髓核細胞生長與分泌之影響
Comparing the Effects of Adipose Tissue-derived Stem Cells (ADSCs) and Bone Marrow-derived Mesenchymal Stem Cells (BMSCs) on the Growth and Secretory Activity of Rabbit Nucleus Pulposus Cells
作者: 郭衡
Kuo, Herng
關鍵字: 脂肪幹細胞;Adipose Tissue-derived Stem Cells;骨髓基質幹細胞;髓核細胞;共同培養;Bone Marrow-derived Mesenchymal Stem Cells;Nucleus Pulposus Cells;Coculture system
出版社: 獸醫學系暨研究所
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摘要: 
椎間盤的退化係由於椎間盤中央髓核組織內細胞合成分泌的proteoglycan減少,使得組織鎖水能力下降,連帶降低椎間盤所能承受的身體壓力,而造成椎間盤的傷害所致。有研究顯示將兔子的骨髓基質幹細胞與其椎間盤髓核細胞共同培養,能提升髓核細胞的活性,包括其細胞增殖能力,以及合成分泌胞外基質的能力,而脂肪組織與骨髓同樣源自於中胚層,在體內含量豐富且較骨髓容易取得。近來有研究證實存在於脂肪中的脂肪幹細胞與骨髓基質幹細胞具有相似的細胞特性及分化能力。因此,本實驗想利用組織化學染色及酵素免疫分析法(ELISA),比較骨髓基質幹細胞與脂肪幹細胞對於椎間盤髓核細胞在細胞增殖、胞外基質分泌等功能上的影響。實驗結果顯示,骨髓基質幹細胞與脂肪幹細胞均能提升髓核細胞的細胞增殖能力;然而在合成分泌胞外基質功能的表現方面,則僅有骨髓細胞具有明顯的刺激作用。故我們推測對於椎間盤退化的修復治療方面,骨髓基質幹細胞應該仍是較理想的選擇。

Intervertebral disc degeneration is due to a decrease of proteoglycan which impairs the ability of nucleus pulposus to imbibe water and reduces the normal load bearing ability of the disc. The studies of disc degeneration treatment had proved that coculture with bone marrow-derived mesenchymal stem cells (BMSCs) raised efficacy in activating nucleus pulposus cells (NPCs), including upregulation of cell proliferation and extracellular matrix production. Adipose tissue-derived stem cells (ADSCs) arise from mesenchyma, unlike BMSCs, are easier to obtain. Many researchers demonstrated that the BMSCs and ADSCs had an equal potential to differentiate into cells and tissues of mesodermal origin. In this study, histochemistry and ELISA were used to compare the effects of cell proliferation, and extracellular matrix production on NPCs when cocultured with BMSCs and ADSCs. Results indicated that both BMSCs and ADSCs raised efficacy in cell proliferation of NPCs. Furthermore, BMSCs could promote extracellular matrix production of NPCs, but the effect was not conspicuous when NPCs were cocultured with ADSCs. In conclusion, we suggest that BMSCs are still more effective on increasing the proliferative and matrix synthesis capacity of NPCs.
URI: http://hdl.handle.net/11455/13968
其他識別: U0005-2607200918491300
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