Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/14041
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dc.contributor.advisor王俊秀zh_TW
dc.contributor.advisorShu-Peng Hoen_US
dc.contributor.advisor何素鵬zh_TW
dc.contributor.author林渝翔zh_TW
dc.contributor.authorLin, Yu-Shyangen_US
dc.date2003zh_TW
dc.date.accessioned2014-06-06T06:51:37Z-
dc.date.available2014-06-06T06:51:37Z-
dc.identifier.urihttp://hdl.handle.net/11455/14041-
dc.description.abstract隱孢子蟲(Cryptosporidium sp.)屬於原蟲性寄生蟲,是在脊椎動物消化道寄生的人畜共通感染性寄生病原,造成宿主下痢症狀為主。本研究針對台灣食蟲性蝙蝠做一系列寄生蟲檢查,發現其中24處棲所洞穴的糞便都可分離出隱孢子蟲卵囊。針對野外採集的台灣葉鼻蝠進行每日糞中隱孢子蟲卵囊數量變化的長期監控,發現糞中可檢出數量較多的隱孢子蟲卵囊,證實台灣葉鼻蝠受隱孢子蟲感染。 於調查過程中,台中縣一處蝙蝠夜間棲所旁有3場水鹿養殖場,每場都有鹿隻受隱孢子蟲感染,其中一場還設有一傳統堆置的小型堆肥場,經抽樣檢查可檢出隱孢子蟲卵囊,與另外12場現代化高溫發酵堆肥場比較,顯示傳統糞便堆置方式無法有效分解隱孢子蟲卵囊。 為瞭解水鹿受隱孢子蟲感染情況,針對中部地區水鹿飼養場進行抽樣,採集17場,76隻水鹿的糞便,66隻水鹿糞便可檢出隱孢子蟲卵囊,每場都可檢出隱孢子蟲卵囊,分析其原因可能是糞便與草料混在地上造成伺機感染。台中縣與南投縣境內牧場所產生的禽畜糞便,大都經私下堆置處理,當糞便中含有隱孢子蟲卵囊時,依文獻報告卵囊可經食糞甲蟲或蒼蠅等昆蟲攜帶,可能藉由食蟲性鳥類與動物食入,經消化排泄因而成為隱孢子蟲發散源,造成隱孢子蟲伺機感染其他動物。zh_TW
dc.description.abstractCryptosporidium spp., the zoonotic infective parasitic protozoa in the digestive dust of vertebrates, is responsible for the clinical symptom of diarrhea. This study aims at a series of examination of parasites in insectivorous bats and Cryptosporidium oocysts were examined in the fecal samples from 24 bat habitats. After long observation of daily feces of the wild caged bats, discovered the existence of Cryptosporidium spp, which indicates these bats were infected. During the survey, the oocysts of Cryptosporidium spp observeed in the feces of 3 deer farms near a nocturnal bat habitat in Taichung county were presented positive for Cryptosporidium infection. The sample from a traditional compost heap in the deer farm was also examined as positive. Compared with the result of those from 12 modem compost heaps, the temperature of traditional compost heap is too low to destroy the Cryptosporidium oocysts. To survey for infection of deer by Cryptosporidium, the feces of deer from 17 daily farms was examined in central Taiwan. Among the 76 deer examined, 66 (86.8%) are observed to be positive for Cryptosporidium infection respectly. The cause may be mixture of fodder with feces. Since the feces of farm animals in central Taiwan have been stocked confidentially, those compost heap containing cryptosporidium oocysts may be carried by the wild filth flies, dung beetles and other insects which will be eaten by insectivorous animals and digested. Therefore, Cryptosporidium oocysts may in this way spread infection.en_US
dc.description.tableofcontents目次……………………………………………………………………………Ⅰ 表次……………………………………………………………………………Ⅴ 圖次……………………………………………………………………………Ⅵ 中文摘要………………………………………………………………………Ⅷ 英文摘要………………………………………………………………………Ⅸ 第一章前言…………………………………………………………………… 1 第二章 文獻探討………………………………………………………………3 (一)隱孢子蟲種類由來……………………………………………………… 3 (二)隱孢子蟲之分類………………………………………………………… 4 (三)隱孢子蟲之生活史……………………………………………………… 5 (四)宿主種類與範圍………………………………………………………… 7 (五)診斷………………………………………………………………………13 1.活體標本(Biopsy) ……………………………………………………… 13 2.卵囊的偵測…………………………………………………………………13 3.免疫方面的偵測法…………………………………………………………14 4.Molecular techniques……………………………………………………14 (六)隱孢子蟲活性分析………………………………………………………17 1.實驗動物接種………………………………………………………………17 2.卵囊脫囊比率………………………………………………………………17 3.FISH法………………………………………………………………………18 4.螢光染劑……………………………………………………………………18 (七)治療與控制………………………………………………………………18 (八)蝙蝠習性介紹……………………………………………………………21 第三章 材料與方法………………………………………………………… 25 一﹑蝙蝠之隱孢子蟲調查……………………………………………………25 (一)蝙蝠棲所洞內地上糞便…………………………………………………25 1.調查對象與糞材採集………………………………………………………25 2.糞材濃縮及飽和蔗糖溶液浮游法…………………………………………25 3.Modified Ziehl-Neelsen Karbofuchsin Stain Method染色檢查……26 4.螢光抗體染色檢查法之免疫學診斷………………………………………26 (二)野外採集之蝙蝠個體觀察………………………………………………26 1.試驗蝙蝠來源與管理………………………………………………………26 2.野外採集之個體……………………………………………………………27 (1)墾丁採集之個體………………………………………………………… 27 (2)南投中寮 3洞採集之個體……………………………………………… 27 (3)南投水里採集之個體…………………………………………………… 28 (4)採集過程死亡之蝙蝠個體……………………………………………… 28 二﹑水鹿個體與其飼養場之隱孢子蟲調查…………………………………28 1.調查對象與糞材採集………………………………………………………28 (1)習慣性下痢之水鹿個體………………………………………………… 28 (2)水鹿飼養場之隱孢子蟲調查…………………………………………… 28 2.糞材濃縮及飽和蔗糖溶液浮游法…………………………………………29 3.Modified Ziehl-Neelsen Karbofuchsin Stain Method染色檢查……29 三﹑堆肥場之隱孢子蟲調查…………………………………………………29 1.調查對象與糞材採集………………………………………………………29 2.糞材濃縮及飽和蔗糖溶液浮游法…………………………………………29 3.Modified Ziehl-Neelsen Karbofuchsin Stain Method染色檢查……29 第四章 結果………………………………………………………………… 30 一﹑蝙蝠之隱孢子蟲調查……………………………………………………30 (一)蝙蝠棲所洞穴地上糞便調查……………………………………………30 1.蝙蝠棲所調查………………………………………………………………30 2.隱孢子蟲卵囊之檢查………………………………………………………31 3.隱孢子蟲卵囊之影像………………………………………………………34 4.螢光抗體染色檢查法之免疫學診斷………………………………………38 (二)野外採集之蝙蝠個體觀察………………………………………………40 1.來自墾丁之個體……………………………………………………………40 2.南投中寮 3洞採集之個體…………………………………………………40 3.南投水里採集之個體………………………………………………………40 4.採集過程死亡之蝙蝠個體…………………………………………………41 二﹑水鹿之隱孢子蟲調查……………………………………………………44 (一)水鹿之隱孢子蟲調查……………………………………………………44 1.水鹿之隱孢子蟲症…………………………………………………………44 2.好發隱孢子蟲症之水鹿飼養場訪查………………………………………45 3.南投縣境內水鹿養殖場調查………………………………………………47 (二)水鹿養殖場週邊環境調查………………………………………………51 1.鹿場周邊堆肥場……………………………………………………………51 2.水鹿養殖場 P旁之蝙蝠活動棲所調查……………………………………53 三﹑堆肥場之隱孢子蟲調查…………………………………………………55 (一)各禽畜糞來源之堆肥場調查……………………………………………55 1. 堆肥場之檢查結果……………………………………………………… 55 2.不同畜產動物之隱孢子蟲卵囊影像………………………………………57 (二)中部家畜糞便堆肥現況…………………………………………………59 1.中部禽畜飼養場糞便之處理方式…………………………………………59 2.中部地區畜產飼養場、堆肥場、蝙蝠棲所之相對位置…………………59 第五章 討論………………………………………………………………… 61 一﹑蝙蝠之隱孢子蟲調查……………………………………………………61 二﹑水鹿之隱孢子蟲調查……………………………………………………64 三﹑堆肥場之隱孢子蟲調查…………………………………………………65 參考文獻………………………………………………………………………69zh_TW
dc.language.isoen_USzh_TW
dc.publisher獸醫學系zh_TW
dc.subject堆肥zh_TW
dc.subjectCryptosporidiumen_US
dc.subject隱孢子蟲zh_TW
dc.title台灣食蟲性蝙蝠之隱孢子蟲調查與堆肥處理對卵囊之影響zh_TW
dc.titleSurvey of Cryptosporidium oocysts in insectivorous bats in Taiwan and the influence of compost heaps treatment on Cryptosporidium oocysts.en_US
dc.typeThesis and Dissertationzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeThesis and Dissertation-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
Appears in Collections:獸醫學系所
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