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Study the Antihepatoma Effect of Liposomal Berberine in a Murine Xenograft Model
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Biomed Chromatogr 13: 442-444, 1999. Zuo F, Nakamura N, Akao T, et al. Pharmacokinetics of berberine and its main metabolites in conventional and pseudo germ-free rats determined by liquid chromatography/ion trap mass spectrometry. Drug Metab Dispos 34: 2064-2072, 2006.||摘要:||
Liposomes, a spherical vesicle composed of a phosopholipid bilayer membranes, are ideal drug carriers capable of increasing the local concentration of drug in tumors. Liposomes can reduce the amount of chemotherapeutic agents needed, extend the circulation time within the patient while reduce the side effects to normal tissues. To date, the antitumor effect of berberine, the main component of Coptis chinesnsis, has been proved. Since an antitumor drug with limited side effects is worth developing, my work focuses on assessing the therapeutic effects of liposome encapsulated berberine (liposomal berberine) on human hepatic carcinoma in a murine xenograft model. HepG2 hepatoma cells were injected subcutaneously into nude mice before following a regimented treatment of one dose every three days treatment. The results of hematology and serum biochemistry showed no remarkable side effects caused by liposomal berberine and berberine, whereas traditional chemotherapeutic drug doxorubicin induced both an inflammatory response and hepatocellular injury. Furthermore, there was no abnormal result from the histopathology examination after the liposomal berberine and berberine treatments, whereas testicular atrophy caused by doxorubicin was observed. At the end of this experiment, both the liposomal berberine and berine treatment groups demonstrated slight decrease in both tumor size and mass when compared with the negative control group (PBS). Only the tumor size and mass of the positive control (doxorubicin) differed significantly from the PBS group. Immunohistopathology examination of CD31 was not influenced by the treatments, whereas berberine significantly decreased the CPI of PNCA, while liposomal berberine significantly decreased the secretion of COX-2. Additionally, liposome could delay the elimination rate in plasma and in different tissues. Since the antihepatoma effects of liposomal berberine were insignificant, further studies are needed to investigate the factors which influence the antihepatoma effects of liposomal berberine, in order to enhance its therapeutic effect.
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