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The effects of trivalent chromium on healing of surgical wound in mice
|關鍵字:||鉻離子;chromium;傷口癒合;小鼠;類固醇;類胰島素生長因子;wound healing;mice;dexamethasone;IGF-1||出版社:||獸醫學系暨研究所||引用:||1. Anderson RA. Stress effects on chromium nutrition of humans and farm animals, in Lyons TP, ed. Biotechnology in the Feed Industry. Proceedings of Alltech’s Tenth symposium. Nottingham, UK, University Press, 267-274, 1994. 2. Anderson RA. Nutritional factors influencing the glucose/insulin system : Chromium. J Am Col Nutr 16: 404-410, 1997. 3. Anderson RA. Recent advances in the clinical and biochemical manifestations of chromium deficiency in human and animal nutrition. J Trace Elem Med Biol 11: 241-250, 1998. 4. Anderson RA. Chromium in the prevention and control of diabetes. Diabetes & Metabolism 26: 22-27, 2000. 5. Anderson RA. Chromium in human nutrition. Nutr Rev 46(7): 271-272, 1988. 6. Anderson RA, Bryden NA, Polansky MM, Deuster PA. Exercise effects on chromium excretion of trained and untrained men comsuming a constant diet. J Appl Physiol 64: 249-252, 1988. 7. 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本實驗為研究三價鉻離子對於小鼠傷口癒合的影響。傷口癒合為一個複雜進行的連續過程，期間有許多細胞及生長因子參與其中。最近的研究發現三價鉻離子對於傷口癒合過程中增殖期的重要生長素─類胰島素生長因子 (Insulin-likie growth factor 1, IGF-1)有增強的作用。根據研究，我們假設鉻離子在體內荷爾蒙上可增加IGF-1的表現及減低類固醇 (Cortisol)的濃度，進而幫助傷口癒合的過程。本實驗使用96隻雄性C57BL/6J小鼠，分成4組，每組24隻並分別給予不同的藥物。第一組灌食生理食鹽水作為控制組，第二及第三組分別灌食三氯化鉻(CrCl3)及類固醇 (Dexamethasone)；第四組則同時給予相同劑量之三氯化鉻及類固醇。經過一週的藥物給予後，所有動物皆於背上切創一道傷口，術後每天持續灌食口服藥物直到犧牲日。於術後第7、14、21及28日每組犧牲6隻小鼠。測量傷口大小並採集皮膚傷口做組織學判讀，臟器組織收集做鉻離子濃度分析，血清檢體做IGF-1濃度分析。傷口測量的結果發現類固醇顯著延遲了傷口癒合，而鉻離子卻加速了傷口癒合。組織切片結果中發現類固醇顯著延遲了炎症反應、再上皮化、肉芽組織生成、血管新生以及膠原纖維沉積；然而鉻離子則有反轉類固醇所造成之不良影響的現象。血清分析結果發現類固醇顯著減少IGF-1的濃度，但可由添加鉻離子而反轉。綜合以上結果，鉻離子可經由調節體內內分泌如IGF-1的表現而加速傷口癒合，當動物面臨緊迫或給予類固醇治療時，此效果可更加顯著。
This study was conducted to investigate the effects of trivalent chromium (Cr3+) on wound healing in mice. Wound healing is a complex event involving many cells and growth factors. Recent study revealed that expression of insulin-like growth factor 1 (IGF-1), one of the key growth factors in proliferative cascade can be enhanced by Cr3+. Based on studies, we postulated that Cr could be of helpful on wound healing by increasing the level of IGF-1 and decreasing the level of serum cortisol on hormone to accelerate the wound healing process. A total of 96 male C57BL6/J mice were randomly assigned to four groups (n=24/group). The first group was treated with normal saline as control, the second and third groups were treated for chromium chloride (CrCl3) and dexamethasone (Dex), respectively. The combination of CrCl3 and Dex were applied to the fourth group. All animals underwent surgical incision on their dorsal region after one week of treatment, the regimen of treatment were continued until the day of sacrifice. Six mice of each group were sacrificed on day 7, 14, 21 and 28 after surgery. Skin wound area were measured and collected for histology evaluation, tissue of organs were collected for Cr concentration analysis, serum concentrations of IGF-I were determined as well. The measurement revealed the delayed healing in Dex-treated group, and an accelerated healing was observed in both CrCl3-treated groups. In histology, the delayed inflammation process, reepithelization, granulation tissue formation as well as collagen deposition were observed in Dex-treated groups. However, reversed detrimental effects of Dex were observed in CrCl3-treated groups. The concentrations of IGF-1 in Dex-treated groups revealed significant decrement compared to other groups, but Cr reversed this effect. Taken up together, Cr accelerated wound healing in surgical wound model in mice through the regulation of hormone concentrations such as IGF-1, the effects could be more prominent when animal is under stress or treated by exogenous glucocorticoids.
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