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Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/14372
標題: 促性腺激素釋放素對卵巢子宮摘除之大鼠腦下垂體荷爾蒙分泌的影響
The response of pituitary hormones secretion after gonadotropin releasing hormone stimulation test in ovariohysterectomized rats
作者: 徐莉雪
Shiu, Li-Shiue
關鍵字: 促性腺激素釋放素;GnRH;卵巢子宮摘除;腦下垂體荷爾蒙;ovariohysterectomized;pituitary hormones
出版社: 獸醫學系暨研究所
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摘要: 
節育已經被證實為導致肥胖的主要風險因子之一。而肥胖與多種內分泌及體內循環賀爾蒙濃度的變化都有很大的相關。促性腺激素釋放素不僅為一主要調節性腺功能的下視丘釋放激素，也被認為可能具有影響促性腺激素分泌細胞以外的其他腦下垂體前葉分泌細胞的功能。但關於促性腺激素釋放素對其他腦下垂體賀爾蒙的影響仍然不清楚。本研究欲探討節育前後腦下垂體賀爾蒙產生的變化，及此變化是否會因額外給予促性腺激素釋放素有所影響，最後節育後的肥胖是否與此腦下垂體賀爾蒙的變化相關。本實驗中將雌性大鼠於30週齡時進行卵巢子宮摘除術，之後依照術後時間的不同再細分成三組，分別為術後20週、24週及30週，另外未手術的母鼠作為對照組。最後進行促性腺激素釋放素刺激試驗，採集給藥前後的血液檢測生長激素、類胰島素生長因子、促腎上腺皮質激素及促甲狀腺激素的濃度。結果，首先比較給予促性腺激素釋放素前基礎腦下垂體賀爾蒙於手術前後的變化。生長激素有隨著子宮卵巢摘除的時間較久而下降的趨勢，且對照組的生長激素濃度皆高於各實驗組，但未達顯著差異。實驗組基礎促腎上腺皮質激素濃度皆顯著高於對照組，程度上可達到三至五倍。接著比較給予促性腺激素釋放素後各組腦下垂體賀爾蒙的變化。各組給藥後30分鐘生長激素濃度高於給藥前，但未達統計上意義。各組給藥後30分鐘類胰島素生長因子濃度皆顯著低於對照組。而各組促腎上腺皮質激素及促甲狀腺激素的濃度於給藥後30分鐘顯著高於給藥前。體重與基礎生長激素濃度呈現顯著負相關 (r = -0.322, P < 0.05)，但與基礎類胰島素生長因子 (r = 0.346, P < 0.05) 及促腎上腺皮質激素 (r = 0.372, P < 0.05) 呈現顯著正相關。結論，給予促性腺激素釋放素不論在性腺摘除前後都會增加其他腦下垂體賀爾蒙的分泌，其中又以促腎上腺皮質激素差異最為顯著。最後由於與體重的相關性推論促腎上腺皮質激素與類胰島素生長因子濃度的上升可能有利於節育後肥胖的形成。

Neutralization has been supposed as one of the main risk factors of obesity. Obesity associated with multiple endocrine alterations in the concentration of circulating hormones. Gonadotropin-releasing hormone (GnRH) is not only a primary regulator of gonadal function but also a possible factor which affects other pituitary cells out of gonadotrophs. Recently, it has been reported that GnRH might induce GH releasing, but the effects on GH and other pituitary hormones of GnRH still remained controversial. In this study, ovariohysterectomy (OHE) was performed in the 30 week-old female Sprague Dawley (SD) rats as experimental groups. Intact female rats were taken as control group. All rats were examined by GnRH stimulation test at different weeks (20, 24, and 30) after OHE. Evaluate the circulating GH, IGF-I, ACTH, TSH concentrations at different time points with commercial ELISA kits. In results, the levels of GH after stimulation in all OHE groups tended to lower than control but without significantly difference. In all groups, the levels of IGF-I at 30 min after stimulation were significantly lower than the basal IGF-I levels. The levels of ACTH at 30 min after GnRH administrating were significantly higher than the basal ACTH levels in all groups. Furthermore, both the basal and stimulated ACTH levels in all OHE groups were significantly higher than the control ones. The levels of TSH after stimulation in all OHE groups tended to higher than control ones but without significant difference. According to the results, GnRH administration could induce GH, ACTH and TSH secretion but reduce IGF-I levels in ovariohysterectomized rats. Whereas the effects of GnRH on GH secretion was not significant as we expected. The obesity after neutralization in female rats is probably accomplished with the elevation of IGF-I and ACTH.
URI: http://hdl.handle.net/11455/14372
其他識別: U0005-0507201217411900
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