Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/14376
標題: 假性狂犬病毒誘發巨噬細胞發炎反應之研究
Study of the cellular inflammatory response of macrophage induced by pseudorabies virus infection
作者: 洪子涵
Hung, Zih-Hang
關鍵字: 假性狂犬病毒;pseudorabies virus;巨噬細胞;氧化壓力;抗氧化;macrophage;oxidative stress;anti-oxidant
出版社: 獸醫學系暨研究所
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摘要: 
假性狂犬病毒(Pseudorabies virus, PRV)屬於皰疹病毒科的α-皰疹病毒亞科,具有封套的雙股DNA病毒,是造成假性狂犬病(pseudorabies, PR)的致病原。假性狂犬病毒可感染的宿主範圍很廣,大多數的哺乳類動物皆可受到感染,但並不感染人類。假性狂犬病會因豬隻年齡大小而出現不同的症狀,包括:兩週齡以下的小豬感染後死亡率可接近100%、懷孕母豬的流產、成豬感染後造成呼吸道及生殖系統上的疾病,因而造成養豬場重大的經濟損失。先前本實驗室研究發現假性狂犬病毒感染RAW264.7小鼠巨噬細胞後給予抗氧化物質可降低發炎反應的發生,是否病毒感染後誘發細胞內ROS上升而使病毒感染受到影響,目前仍不清楚。因此,本研究將探討假性狂犬病毒(PRV)引起細胞發炎反應及氧化壓力變化之研究。
本實驗首先觀察PRV感染後ROS(reactive oxygen species)變化,使用0.1MOI之PRV感染劑量在感染6小時、12小時及24小時後,不論利用Griess reagent測定一氧化氮(NO)濃度,還是以西方點墨法觀察iNOS蛋白質表現量,結果皆顯示感染24小時有大量NO產生,同時亦可觀察到細胞內有過氧化脂質(MDA)堆積的現象(p<0.05)。以ELISA分析結果發現PRV感染時會產生大量IL-6、MCP-1、TNF-α (p<0.05),並影響信息傳遞路徑:NF-κB、mitogen-activated protein kinase(MAPK)路徑及JAK/STAT路徑的蛋白質表現。此外,為了瞭解ROS產生的途徑,利用西方墨點法觀察NADPH oxidase、heme oxygenase-1(HO-1)、AMPK及Nrf-2磷酸化表現情形,結果顯示PRV感染後皆可增加其表現量。另一方面,為了證實PRV感染會調控細胞內抗氧化系統,測定抗氧化酵素SOD、catalase及GPx的酵素活性,並以西方墨點法觀察其蛋白質表現量變化,結果發現PRV感染可增加抗氧化酵素SOD、catalase及GPx的表現。本研究結果顯示假性狂犬病毒感染對於巨噬細胞的影響可能與ROS及若干轉錄因子的活化有關,有助於未來了解假性狂犬病毒感染造成發炎反應機轉的研究。

Pseudorabies virus (PRV), a member of the alpha-herpesvirinae subfamily in the family Herpesviridae, is the causative agent of Aujeszky’s disease (AD, pseudorabies). PRV has a broad host range infecting most mammals except humans. The severity of clinical signs depends on the age of the pig. Young piglets are highly susceptible with mortality rates reaching 100% during the first 2 weeks of age. Infected adult pig shows primarily respiratory and reproductive diseases. In previous study, antioxidants can regulate PRV-induced inflammatory response in RAW264.7 cells. The relationship between virus infection and ROS (reactive oxygen species) production is still unclear. In this study, we investigated the inflammatory response and the status of oxidant/antioxidant system in PRV-infected RAW264.7 cells. To investigate intracellular ROS production induced by PRV, RAW264.7 cells were infected with PRV at 0.1 multiplicity of infection for 6 hours, 12 hours and 24 hours. Results indicated a significant increase of NO, TNF-α, IL-6, and MCP-1(p<0.05), and an increased level of NF-κB, ERK, p38 and JNK MAPKs in PRV-infected RAW264.7 cells. Moreover, malondialdehyde (MDA), an oxidative stress marker, is also increased(p<0.05). Western blotting showed that NADPH oxidase, heme oxygenase-1, AMPK and Nrf2 level were up-regulated. Our data also showed that PRV infection induced antioxidant enzyme response, such as SOD, catalase and GPx. In conclusion, ROS production and several transcription factor obviously play important roles on the inflammatory responses in PRV-infected RWA264.7 cells.
URI: http://hdl.handle.net/11455/14376
其他識別: U0005-2207201314304900
Appears in Collections:獸醫學系所

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